BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,298,720	C→T	G35E (GGA→GAA)	yraQ ←	permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,298,720	0	C	T	98.3%	1242.6 / NA	349	G35E (GGA→GAA)	yraQ	permease
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (138/204); minor base G (5/1); total (143/206)
Fisher's exact test for biased strand distribution p-value = 4.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 350 total aligned reads displayed.

CCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAG  >  NZ_CP025268/3298583‑3298856

  gcTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCa                                                                                                                                        <  2:1052105/140‑1 (MQ=255)

   cTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAt                                                                                                                                       >  1:1300527/1‑140 (MQ=255)

    tttCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAtt                                                                                                                                      <  1:4818250/140‑1 (MQ=255)

     ttCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGGTATACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCAttt                                                                                                                                     >  1:4238340/1‑140 (MQ=255)

         aTACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTcaca                                                                                                                                 <  2:4204098/140‑1 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                            <  2:67274/140‑1 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                            >  2:3098764/1‑140 (MQ=255)

              gcgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                                                                                                                            <  2:4251963/140‑1 (MQ=255)

              gcgAGGAAATAGAGCATCGCGTAATCCAACGCCGCCTGCCATGCGTTGGCCTCCGCCTGCGCAATGAGAGATTTACCGATACTGTGGGTTCCGGCAGCAGTGATCGCTTAGCAGTAGTACGTATCCCATTTCACATACCa                                                                                                                            >  2:1259003/1‑140 (MQ=255)

               cgAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGGTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                                                                                                                           >  2:2128944/1‑140 (MQ=255)

                  ggAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAgaga                                                                                                                        <  1:723489/140‑1 (MQ=255)

                      aTAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCgg                                                                                                                    >  2:691730/1‑140 (MQ=255)

                          atcatcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGCTAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGCCAAt                                                                                                                <  2:2421561/140‑1 (MQ=255)

                              tctagTAATCGAACGCCGCCTGCCATGGGTGAGCATCCGGGTGCGCAATGATAGGTTTACTGATACTGTGAGTTTCGGGAACAGTGAACGCGTTGACTTAATAAGGTTGGCAGTTGACAAACCAGAGAACAGCAATGata                                                                                                            >  2:213829/5‑138 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                            >  1:2677186/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                            >  1:3940742/1‑140 (MQ=255)

                              tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACa                                                                                                            >  2:4969315/1‑140 (MQ=255)

                               cgcgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTACCATTTCACATACCAGAGACCGGCAATGACaa                                                                                                           <  2:431110/140‑1 (MQ=255)

                                    aaTCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACgaga                                                                                                      <  2:3372121/140‑1 (MQ=255)

                                         aaCGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGagaaagaa                                                                                                 >  2:3873391/1‑140 (MQ=255)

                                              cgccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                                            <  2:3301033/140‑1 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                          >  2:154060/1‑140 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                          >  1:1211328/1‑140 (MQ=255)

                                                ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGcgcg                                                                                          >  1:88241/1‑140 (MQ=255)

                                                      aTGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTc                                                                                    <  1:845194/140‑1 (MQ=255)

                                                           ttAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacca                                                                               <  2:2210713/140‑1 (MQ=255)

                                                             aGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACt                                                                             >  1:4293811/1‑140 (MQ=255)

                                                             aGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACt                                                                             >  1:4105921/1‑140 (MQ=255)

                                                             aGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACt                                                                             >  1:5067677/1‑140 (MQ=255)

                                                              gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTg                                                                            >  1:4616262/1‑140 (MQ=255)

                                                                   cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATgg                                                                       >  1:3542040/1‑140 (MQ=255)

                                                                   cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATgg                                                                       >  1:3196556/1‑140 (MQ=255)

                                                                    gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCTGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                      >  2:85949/1‑140 (MQ=255)

                                                                    gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                      >  1:3612013/1‑140 (MQ=255)

                                                                    gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                      >  1:1802206/1‑140 (MQ=255)

                                                                         cgcAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTg                                                                 <  2:2797592/140‑1 (MQ=255)

                                                                         cgcAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTGCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGTAAAGCGCGGGACTCCACCACTGAATGGGCGTTg                                                                 >  2:4452982/1‑140 (MQ=255)

                                                                             aGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTgccgc                                                             <  1:241185/140‑1 (MQ=255)

                                                                               gatatgtttACCGATACTGTGGGCTTCGGCAGCAGTAAGCGCTTTGCCGTAGTAAGGTTCCCAGTTCACATTCCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCTCTTAATGGGCGTTGCCGCCt                                                           <  2:3072232/134‑1 (MQ=255)

                                                                               gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCCTTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                           <  1:3064758/140‑1 (MQ=255)

                                                                               gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                           <  1:3323752/140‑1 (MQ=255)

                                                                               gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                           <  1:4913007/140‑1 (MQ=255)

                                                                               gATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                                           <  2:3542040/140‑1 (MQ=255)

                                                                                    aTTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGAt                                                    <  2:3413691/140‑1 (MQ=255)

                                                                                    aTTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGAt                                                    <  2:3716346/140‑1 (MQ=255)

                                                                                    aTTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGAt                                                    <  1:2605251/140‑1 (MQ=255)

                                                                                        aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCg                                                                    >  2:1657163/1‑122 (MQ=255)

                                                                                        aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAg                                                >  2:3207285/1‑140 (MQ=255)

                                                                                         ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGa                                               >  1:1119820/1‑140 (MQ=255)

                                                                                          cGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGAc                                              >  2:5106938/1‑140 (MQ=255)

                                                                                            aTACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTg                                            <  1:1019742/140‑1 (MQ=255)

                                                                                             tACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGa                                           <  1:2745058/140‑1 (MQ=255)

                                                                                             gaCTGTGGGAGTCGGCAGCTGTAAACGCTGTGCCGTAGTACGGTTCCCATTTAACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGa                                           <  2:1930895/139‑1 (MQ=255)

                                                                                              ccTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGCAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          <  2:1338693/139‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          <  1:3470756/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          <  2:793172/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          <  2:537829/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          <  2:4379679/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          >  1:4993373/1‑140 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          >  1:476117/1‑140 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          <  2:1588112/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          <  2:1170134/140‑1 (MQ=255)

                                                                                              aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGAAAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAc                                          <  1:3907365/140‑1 (MQ=255)

                                                                                               cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACCACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAcc                                         <  1:4048921/140‑1 (MQ=255)

                                                                                               cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAcc                                         <  1:55235/140‑1 (MQ=255)

                                                                                               cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAcc                                         <  2:4000583/140‑1 (MQ=255)

                                                                                               cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAcc                                         <  1:948581/140‑1 (MQ=255)

                                                                                               cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGAcc                                         <  2:2055309/140‑1 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGAGATGAAGACTGAc                                          <  1:2169398/138‑1 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGt                                      >  1:3788196/1‑140 (MQ=255)

                                                                                                  tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGt                                      <  2:3117547/140‑1 (MQ=255)

                                                                                                  tgGGTTGCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGt                                      <  1:3811219/140‑1 (MQ=255)

                                                                                                       ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAg                                 >  2:611309/1‑140 (MQ=255)

                                                                                                       tgcGGCAGCAGTAAAGGCTTTGGCGTAGTAAGGTTGGCATTTCACATACCAGAGACCGGCAATGCCAACGAGAAAGAAAAGCGCGGGTTTCCACCGCTGAATGGGCGTTGCGGTCT‑‑GAGATGAGGACTGAGCAGTCATAg                                 >  2:2683206/3‑140 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCa                               <  1:1365719/140‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCa                               <  1:4358381/140‑1 (MQ=255)

                                                                                                         cGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCa                               <  1:1763949/140‑1 (MQ=255)

                                                                                                           gcagaaGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCAtt                             <  1:2926876/140‑1 (MQ=255)

                                                                                                              gcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTccc                          <  1:157351/140‑1 (MQ=255)

                                                                                                              gcagTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTccc                          >  1:1652853/1‑140 (MQ=255)

                                                                                                                    aaCGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGaa                    <  2:4667254/140‑1 (MQ=255)

                                                                                                                    aaCGCTTTGCAGTAGTAAGGTACCCATTTCACATAAAAAAGACCGGCCATGACAACGAGAAAGAAAAGCGAGGGTTTCCACACCTCAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTACCCAGGaa                    <  2:10964/140‑1 (MQ=255)

                                                                                                                     aCGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAAt                   <  1:3098822/140‑1 (MQ=255)

                                                                                                                     aCGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAAt                   <  1:4681494/140‑1 (MQ=255)

                                                                                                                     aCGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCCGCAATGACAACGAGAAAGAAAAGCGCGGTTTGCCACCACTGACTGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCGGTCATAGCATTCCCCAGGAAt                   <  2:2837843/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAATGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAACGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:3811622/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCTTTCCCCAGGAATAAGt               >  1:2838677/1‑140 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:5066969/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:3669655/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  1:2279134/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  1:2647243/140‑1 (MQ=255)

                                                                                                                         tttGCCGTAGTAAGGTTCCCAGTTCAGATAGCAGAGACCAGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGAGCGTTGCCGGCT‑‑GAAATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGt               <  2:2582132/140‑1 (MQ=255)

                                                                                                                          gtGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAAAGAGAAAGAAAAGCGCGGGGTTCCAACACTGAATGGGCGTTGCCGAAT‑‑GAGATGAAGACAGACGAGTAATAGCATGCCACAGGAAttattg              >  2:1736179/2‑135 (MQ=255)

                                                                                                                           tGCCGTAGTAAGGTTCCCATTTCACATACTAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTGTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAAGAATAAGTTa             >  2:1228827/1‑140 (MQ=255)

                                                                                                                           tGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa             >  1:2218380/1‑140 (MQ=255)

                                                                                                                           tGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa             <  1:1408263/140‑1 (MQ=255)

                                                                                                                           tGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa             >  1:3980540/1‑140 (MQ=255)

                                                                                                                                     aGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGa   <  2:4941874/140‑1 (MQ=255)

                                                                                                                                     aGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGGTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGa   >  2:2910100/1‑140 (MQ=255)

                                                                                                                                      ggTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAg  <  2:1775471/140‑1 (MQ=255)

CCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCT‑‑GAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTGAG  >  NZ_CP025268/3298583‑3298856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`