BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,308,725	T→C	295 (TAA→TAG)	nlpI ←	lipoprotein NlpI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,308,725	0	T	C	99.4%	1133.6 / NA	342	295 (TAA→TAG)	nlpI	lipoprotein NlpI
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (167/173); total (167/175)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

CTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTC  >  NZ_CP025268/3308589‑3308859

cTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGGTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAGAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTAt                                                                                                                                     >  2:4058526/1‑140 (MQ=255)

 tcatcaTGAAATTGATCAGCAATTTGCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTAtt                                                                                                                                    >  2:4597469/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGc                                                                                                                                  >  2:3854918/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGc                                                                                                                                  >  2:1166150/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGc                                                                                                                                  >  2:295723/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGc                                                                                                                                  >  2:1978247/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGc                                                                                                                                  >  2:2662232/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGc                                                                                                                                  >  1:2939388/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGCGTA‑‑‑CGTCAGCTATTGc                                                                                                                                  >  1:2842351/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGAAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGc                                                                                                                                  >  2:866571/1‑140 (MQ=255)

     catGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTg                                                                                                                                >  1:2972216/1‑140 (MQ=255)

     catGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTg                                                                                                                                >  1:606112/1‑140 (MQ=255)

     catGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTg                                                                                                                                >  1:4433543/1‑140 (MQ=255)

       tGAAATTGCTCAGCAATTCTCATTGCAAAGTGTGAACCGGCTCAAAGTAGGTGTACTAACGAATAACCACGCCCAGCCCCGTTAAGGTGATTGAAATCAATATGGATTACGGGCTGATGTGTA‑‑‑CGTTATTTATTGCTGGt                                                                                                                              <  2:2591594/140‑1 (MQ=255)

           aTTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGa                                                                                                                          <  1:4267022/140‑1 (MQ=255)

             tGATTAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTGACGAACAACAACGCCCTCACCAGTTAAGGTGATGGCAATC‑‑AAAAGATTACGGGCTGATGTTTATGTCGTCAGCTATTGCTGGTCCGAt                                                                                                                         >  2:5076068/1‑140 (MQ=255)

             tGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGTTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGAtt                                                                                                                        >  2:2882673/1‑140 (MQ=255)

             tGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGAtt                                                                                                                        <  2:4349047/140‑1 (MQ=255)

             tGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGAtt                                                                                                                        >  2:205849/1‑140 (MQ=255)

               aTCAGCAATTTTCATTGCAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTTTA‑‑‑CGTCAGCTATTGCTGGTTCGATTCt                                                                                                                      >  2:4208995/1‑140 (MQ=255)

               aTCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACACGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCt                                                                                                                      >  2:4084250/1‑140 (MQ=255)

                 cAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGc                                                                                                                    <  2:4491344/140‑1 (MQ=255)

                  aGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGcc                                                                                                                   <  2:372959/140‑1 (MQ=255)

                    cAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CTTCAGCTATTGCTGGTCCGATTCTGCCAg                                                                                                                 >  2:2789250/1‑140 (MQ=255)

                     aaTTTTCATTGAAAAGGGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAgg                                                                                                                <  2:3441260/140‑1 (MQ=255)

                      aTTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGt                                                                                                               <  1:134726/140‑1 (MQ=255)

                         ttCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCAt                                                                                                            >  2:710148/1‑140 (MQ=255)

                           cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                          <  1:923006/140‑1 (MQ=255)

                           cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                          <  1:1468891/140‑1 (MQ=255)

                           cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                          <  2:4379985/140‑1 (MQ=255)

                              tGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTACGATTCTGCCAGGTCATCTTgg                                                                                                       >  2:2833313/1‑140 (MQ=255)

                                aaaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTc                                                                                                     <  2:3680526/140‑1 (MQ=255)

                                 aaaGTGTGAACCGGCTCAACGTAGGTGTATTAACGACCCACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAACAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                    <  2:2574476/140‑1 (MQ=255)

                                 aaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                    <  1:3262613/140‑1 (MQ=255)

                                 aaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                    <  1:2339235/140‑1 (MQ=255)

                                 aaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                    <  2:238086/140‑1 (MQ=255)

                                 aaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                    <  1:3284228/140‑1 (MQ=255)

                                 aaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                    <  1:1688149/140‑1 (MQ=255)

                                      gtgAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGcc                                                                                               >  2:1360929/1‑140 (MQ=255)

                                          aCCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAgg                                                                                           <  2:103417/140‑1 (MQ=255)

                                              gCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                       <  2:1764844/140‑1 (MQ=255)

                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGa                                                                                      >  1:5105278/1‑140 (MQ=255)

                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGa                                                                                      >  1:4110490/1‑140 (MQ=255)

                                                tCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                                                     <  2:49006/140‑1 (MQ=255)

                                                  ataGTAGGTGTATTAACGATCAACAACGCCCTCCCCCGTTAAGGTGATGGCAATCAAACAAGATTACGGGCTGATGTGTA‑‑‑CGGCAGCTATGGCTGGTACGATTCTGCCACGTCATCCTGGTCCTGGCCCAGGAGCGAtcc                                                                                   <  2:2506126/138‑3 (MQ=255)

                                                   aaGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAAt                                                                                  <  1:1135606/140‑1 (MQ=255)

                                                       aGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCca                                                                              <  1:3904947/140‑1 (MQ=255)

                                                         gtgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaac                                                                            >  2:790424/1‑140 (MQ=255)

                                                         gtgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaac                                                                            >  2:2692996/1‑140 (MQ=255)

                                                         gtgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAACGATAATTCcaac                                                                            >  2:2298886/1‑140 (MQ=255)

                                                          tgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaaca                                                                           <  2:3380538/140‑1 (MQ=255)

                                                            tATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAAt                                                                         >  1:2259698/1‑140 (MQ=255)

                                                              ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGc                                                                       >  1:707250/1‑140 (MQ=255)

                                                              ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGGCCTCGCCCAGGAGCGATAATTCCAACAATGc                                                                       >  1:3608294/1‑140 (MQ=255)

                                                              ttAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCAGCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGc                                                                       >  1:1635254/1‑140 (MQ=255)

                                                                  cGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                                   >  1:1733235/1‑140 (MQ=255)

                                                                  cGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTAt                                                                   <  1:3944491/140‑1 (MQ=255)

                                                                  cGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAACGCGTAt                                                                   <  2:4530577/140‑1 (MQ=255)

                                                                    aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCg                                                                 >  2:1717789/1‑140 (MQ=255)

                                                                    aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGGCCTGGCCGAGGAGCGGTAATTCCAGCAATGCGTATCg                                                                 >  2:3912809/1‑140 (MQ=255)

                                                                      caacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGt                                                               >  2:899019/1‑140 (MQ=255)

                                                                       cacatcccccTCACCCGTTAAGGTGATGGTAATCAAAAAAGATTCCGGGCTGTTGTGTC‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                              <  2:3169462/133‑1 (MQ=255)

                                                                       aacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                              <  1:867459/140‑1 (MQ=255)

                                                                              cccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaaca                                                       <  1:4973596/140‑1 (MQ=255)

                                                                                 tCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGTCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAg                                                    >  1:2780083/1‑140 (MQ=255)

                                                                                   aCCCGTTAAGGTGATGGCAATCAAAAAAGATTCCGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGtt                                                  >  1:4335055/1‑140 (MQ=255)

                                                                                        ttAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                             <  1:2643930/140‑1 (MQ=255)

                                                                                         tAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAAc                                            >  1:751921/1‑140 (MQ=255)

                                                                                             gTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                    >  1:3795024/1‑80 (MQ=255)

                                                                                             gTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACgttg                                        >  1:259957/1‑140 (MQ=255)

                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGCTGTGGA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAAGACCAACAACGCGTATCGCTGCTCAACGAAGTTATGAACggtgct                                      >  2:2115406/1‑138 (MQ=255)

                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATTAACgttgtt                                      >  2:44229/1‑140 (MQ=255)

                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACgttgtt                                      >  2:1442301/1‑140 (MQ=255)

                                                                                                aTGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGttgttg                                     >  1:4984541/1‑140 (MQ=255)

                                                                                                  ggCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGc                                   >  1:4731037/1‑140 (MQ=255)

                                                                                                  ggCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGc                                   >  1:4616656/1‑140 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTTATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                              <  2:1536304/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGTGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                              <  2:4711495/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                              <  1:3544566/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                              <  1:1975906/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATCCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                              <  1:3076911/140‑1 (MQ=255)

                                                                                                       gcAAAAAAGATTACGGGCCGATTTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCAGCTTGGTCCTGGCCCAGGAGCTATTATGCCAACAATGGGGATCAGTGCTCAACAGAGTGATGAAGGATGTCGTCAAccc                              >  2:3075428/2‑139 (MQ=255)

                                                                                                            aaaGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGt                         <  1:1487672/140‑1 (MQ=255)

                                                                                                            aaaGATTACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGt                         <  2:4663629/140‑1 (MQ=255)

                                                                                                                 ttACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGCGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                    <  2:47670/140‑1 (MQ=255)

                                                                                                                 ttACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                    <  1:2006882/140‑1 (MQ=255)

                                                                                                                 ttACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                    <  1:3873454/140‑1 (MQ=255)

                                                                                                                 ttACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                    <  1:4533581/140‑1 (MQ=255)

                                                                                                                 ttACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                    <  2:3310759/140‑1 (MQ=255)

                                                                                                                 ttACGGGCTGATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTCCCAGGTCATCTTGGTCCTGCCCCAGGAGTCATAATTCCAACGATGCGTGTCGGTGCTCGACGAAGTTATGAACGTTGGTGGGAACCGCCAGTTTGaa                    <  2:1237226/140‑1 (MQ=255)

                                                                                                                          gATGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGt           >  1:3722350/1‑140 (MQ=255)

                                                                                                                           aTGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTg          >  2:1862512/1‑140 (MQ=255)

                                                                                                                           aTGTGTA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTg          >  2:807974/1‑140 (MQ=255)

                                                                                                                              tgtA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcg       <  1:487425/140‑1 (MQ=255)

                                                                                                                              tgtA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcg       <  1:3976871/140‑1 (MQ=255)

                                                                                                                               gtA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcgc      <  1:4208995/140‑1 (MQ=255)

                                                                                                                               gtA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGAGTCTCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGACGTGgcgc      <  1:10218/140‑1 (MQ=255)

                                                                                                                               gtA‑‑‑CGTCAACTCTTGCTGCTCCGTTTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATACTTCCCACAATGCGTATCGGTGCTCAACAAAGCTATTACCGTTGTTGGCAACCCCCAGTTTGAACAGTGCCGTGgcgc      <  2:1577942/140‑1 (MQ=255)

                                                                                                                                tA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCt     >  1:2506580/1‑140 (MQ=255)

                                                                                                                                tA‑‑‑CGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCt     >  2:2934830/1‑140 (MQ=255)

CTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTA‑‑‑CGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTC  >  NZ_CP025268/3308589‑3308859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`