BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	345,338	C→T	L17L (TTG→TTA)	yahN ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	345,338	0	C	T	99.4%	600.3 / NA	161	L17L (TTG→TTA)	yahN	LysE family transporter
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (76/83); total (77/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACT  >  NZ_CP025268/345201‑345471

cccAGCGCCACGCCCATCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTCTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAATGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                     <  1:2828093/140‑1 (MQ=255)

cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                     <  2:5021932/140‑1 (MQ=255)

cccAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                     <  1:4105873/140‑1 (MQ=255)

  cAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAg                                                                                                                                   <  1:3790650/140‑1 (MQ=255)

  cAGCGACAAGCCCACGCCGGTCAGCACCCCTGCGCGTCGACCGGAAGACAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCCCGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAg                                                                                                                                   <  2:334132/140‑1 (MQ=255)

     cgcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGAt                                                                                                                                >  1:3120896/1‑140 (MQ=255)

        cACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCa                                                                                                                             <  2:5110604/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGa                                                                                                                                 >  2:2636481/1‑134 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGa                                                                                                                                 <  1:2636481/134‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                           >  1:3048026/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                           >  1:4841811/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                           <  2:1995942/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                           >  1:256050/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                           <  1:2360132/140‑1 (MQ=255)

            cccAGTCCGGTGAGCTCACCTGCCCGTCCATCGGACCCCAGGCTGGTTTGTACTACCCCCTAGTGATTGGATCCCGGATTTAAAAACGTCATCACTAAAAGTCCTACGGTCAGGTAAACGGCATGTAAATGATCCATAGt                                                                                                                         <  2:3617935/140‑1 (MQ=255)

            ccaaGCCCGGTCAGCCCCCCTGCGCGTCGACCGCAAGCCAGGCTGGTTTATACTACCACCAAGGGATTGGGCCCCGGATTAAAAAAAGTAATCACGAACCGTCCTACGGTCAGGTAAACGGCCTGTAAAGGCTCCATAGt                                                                                                                         <  2:3618381/137‑1 (MQ=255)

               aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGTATTAAAAAAAGTAATCACGAACAGGCCTACGGTCAGGTAAACGGCATGTACAGGATCCATTGTGAt                                                                                                                      >  2:215425/1‑140 (MQ=255)

               aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAt                                                                                                                      >  1:2465185/1‑140 (MQ=255)

               aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAt                                                                                                                      >  2:307353/1‑140 (MQ=255)

                gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAtt                                                                                                                     <  1:415643/140‑1 (MQ=255)

                gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAtt                                                                                                                     <  1:416547/140‑1 (MQ=255)

                gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAtt                                                                                                                     <  1:1026465/140‑1 (MQ=255)

                    ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCAt                                                                                                                 <  2:1541501/140‑1 (MQ=255)

                    ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCAt                                                                                                                 <  2:4460785/140‑1 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                >  1:2671627/1‑140 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                >  1:2888313/1‑140 (MQ=255)

                      tCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATcc                                                                                                               >  2:892761/1‑140 (MQ=255)

                         gCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCata                                                                                                            <  1:648773/140‑1 (MQ=255)

                         gCACCCATGCGCGTCGACCGGAACCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTACTCACGAACAGTCCTACGGTCAGGTCAACGGCATGTAAAGGATCCATAGTGATTTCATCCata                                                                                                            <  2:4661856/140‑1 (MQ=255)

                           aCCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaa                                                                                                          >  1:3570158/1‑140 (MQ=255)

                           aCCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaa                                                                                                          >  1:3570173/1‑140 (MQ=255)

                             cccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGCGATTGGATCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGCTCCATAGTGATTTCATCCataaata                                                                                                        <  1:184311/140‑1 (MQ=255)

                              ccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaataa                                                                                                       <  1:2722540/140‑1 (MQ=255)

                              ccTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaataa                                                                                                       <  1:2161001/140‑1 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                      <  1:4734782/140‑1 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                      <  1:637848/140‑1 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGACCTACGGTCAGGTAAACGGCATGAAAAGGATCCATAGAGATTTCATCCATAAATAAg                                                                                                      >  1:223121/1‑140 (MQ=255)

                                   gcgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaa                                                                                                  <  1:5019107/140‑1 (MQ=255)

                                    cgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaac                                                                                                 >  2:4191857/1‑140 (MQ=255)

                                    cgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaac                                                                                                 >  2:91523/1‑140 (MQ=255)

                                       cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                                                              <  1:886878/140‑1 (MQ=255)

                                       cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                                                              <  2:2437596/140‑1 (MQ=255)

                                       cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                                                              >  1:621503/1‑140 (MQ=255)

                                         aCCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaataa                                                                                                       <  1:793476/129‑1 (MQ=255)

                                         aCCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                      >  1:4487383/1‑130 (MQ=255)

                                         aCCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                      <  2:4487383/130‑1 (MQ=255)

                                             gAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcat                                                                                        <  2:3839582/140‑1 (MQ=255)

                                                    ggCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATACGTGAACTAACTGCATCATAttt                                                                                 <  2:4940894/140‑1 (MQ=255)

                                                         gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAcc                                                                            <  1:1835675/140‑1 (MQ=255)

                                                         gTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTAcc                                                                            >  2:4318490/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                  <  1:1257542/140‑1 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                  >  2:4384443/1‑140 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                               >  1:1108472/1‑140 (MQ=255)

                                                                      caAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCggg                                                               >  2:821383/1‑140 (MQ=255)

                                                                        aaGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTg                                                             <  1:2330652/140‑1 (MQ=255)

                                                                          gagaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCg                                                           <  1:4305252/140‑1 (MQ=255)

                                                                           agaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATCAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCga                                                          <  2:888151/140‑1 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataaata                                                                                                        <  2:2684495/92‑1 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                        >  2:1367843/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                        >  2:3811970/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                        >  2:2962308/1‑140 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAGTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                        >  2:1873940/1‑140 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTGAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATAGTTCTACCAAAAATAATCGGGTGCgagag                                                       <  2:4747954/140‑1 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                       <  2:4646103/140‑1 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                       <  1:647643/140‑1 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                       >  2:2907452/1‑140 (MQ=255)

                                                                                  cTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGAt                                                   >  1:1620677/1‑140 (MQ=255)

                                                                                     ccGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcac                                                >  1:2232240/1‑140 (MQ=255)

                                                                                      cGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATCAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                               <  2:4546701/140‑1 (MQ=255)

                                                                                      cGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                               <  1:587260/140‑1 (MQ=255)

                                                                                      cGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                               <  1:3439228/140‑1 (MQ=255)

                                                                                       ggATTAAACAAAGTAATCACGAACACTCCTACCGTCAGGTAAACGGCATGCCAAGGACCCATAGTGATCCCATCCCTAAATCAGCCACCTAGCTCCCTCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaa                                              <  2:1839286/140‑1 (MQ=255)

                                                                                             aaaaaaGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATGAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTc                                        >  2:4708157/1‑140 (MQ=255)

                                                                                                      aTCACGACCAGTCCTACGGGCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAACATAATCGGGTGCGAGAGAGATCACACAGTGTCTTATTTCCg                               >  2:2189949/1‑140 (MQ=255)

                                                                                                              cAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTgg                       <  1:1388477/140‑1 (MQ=255)

                                                                                                              cAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTgg                       <  2:3351892/140‑1 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                    >  1:3389500/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTATACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTGCCGGTTACTGGCGt                    >  2:3912677/1‑140 (MQ=255)

                                                                                                                  ccTACGGTCAGGTAAACGGCATGTAAAGGATCCATGGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                   <  2:3829033/140‑1 (MQ=255)

                                                                                                                        gTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGcc             <  1:4865811/140‑1 (MQ=255)

                                                                                                                        gTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGcc             <  1:2760997/140‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAg                                            <  2:558012/106‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          >  1:4972633/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          <  1:821383/140‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          >  2:779327/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          >  2:4957175/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          >  1:96916/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          >  1:1552033/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          >  1:977882/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          >  2:4385447/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg          >  1:2953793/1‑140 (MQ=255)

                                                                                                                            ggTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctga         <  2:1634287/140‑1 (MQ=255)

                                                                                                                             gTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATGAATAAGTGAACTAACTGCCTAATATTTCTACCAAAAGTAATAGGGTGAGAGAGAGATCACGAAGTGTCTTATTTCGGGTTACTGGCGTTTATGCActgag        >  2:3653023/1‑139 (MQ=255)

                                                                                                                                 aCGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGaa    <  2:755467/140‑1 (MQ=255)

                                                                                                                                 aCGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGaa    <  1:4878887/140‑1 (MQ=255)

                                                                                                                                 aCGGCATGTAAAGGATCCATACTGATTTCATCCATAAATAAGTGACCTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGaa    <  1:1286085/140‑1 (MQ=255)

                                                                                                                                  cGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGGGTTTATGCCCTGACTGAAc   >  1:3302202/1‑140 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt  <  1:3921266/140‑1 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt  >  1:2293043/1‑140 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt  <  1:2582236/140‑1 (MQ=255)

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACT  >  NZ_CP025268/345201‑345471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`