Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,508,628 G→A pseudogene (648/878 nt) php ← phosphotriesterase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,508,6280GA98.4% 1380.4 / NA 378pseudogene (648/878 nt)phpphosphotriesterase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (254/118);  minor base C (2/4);  total (256/122)
Fisher's exact test for biased strand distribution p-value = 8.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 382 total aligned reads displayed.

TAGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGTA  >  NZ_CP025268/3508491‑3508762
                                                                                                                                         |                                                                                                                                      
  gTAAATAGTCATAGCCCTACCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTaa                                                                                                                                    <  2:2678138/140‑1 (MQ=255)
       tAGTCATCGCCATAACCACCGTTGGCTCTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGATTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCCTTTCGTCCTGGTAGTAACtatc                                                                                                                               <  2:2698421/140‑4 (MQ=255)
        aGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTc                                                                                                                              >  1:4786793/1‑140 (MQ=255)
         gTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCGACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCGGGGTAGTAACTGTTCt                                                                                                                             >  1:2838089/1‑140 (MQ=255)
            aTAGCCCTAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGAGATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAACATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCCTGc                                                                                                                          >  2:4858213/1‑140 (MQ=255)
             tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTTcc                                                                                                                         >  2:2037099/1‑140 (MQ=255)
             tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                         >  1:5088829/1‑140 (MQ=255)
             tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                         >  2:2990196/1‑140 (MQ=255)
             tAGCCATAACCACCGTAGGCTTTTAAATCGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGCAGCATCGCACTACGCCTTTCCTCCCGGTAGTAACTGTTCTTccc                                                                                                                         >  2:969248/1‑140 (MQ=255)
               gCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGATGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGa                                                                                                                       <  2:454926/140‑1 (MQ=255)
               gCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGa                                                                                                                       <  1:3896555/140‑1 (MQ=255)
               gCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGa                                                                                                                       <  2:852410/140‑1 (MQ=255)
               gCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGa                                                                                                                       <  2:3263455/140‑1 (MQ=255)
                 cATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATg                                                                                                                     <  1:4169689/140‑1 (MQ=255)
                   tAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgt                                                                                                                   >  2:2429837/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  <  2:836576/140‑1 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  1:1276744/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  1:2926119/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  1:1845879/1‑140 (MQ=255)
                    aaCCACCCTTGGCTTTTAAATGGGAGCGGTGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTGACGGTTCTTGCCGATGgtg                                                                                                                  <  2:3581111/140‑1 (MQ=255)
                       caccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCg                                                                                                               <  2:2641830/140‑1 (MQ=255)
                        accGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGa                                                                                                              <  1:312350/140‑1 (MQ=255)
                         ccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGaa                                                                                                             <  1:4077214/140‑1 (MQ=255)
                         ccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGaa                                                                                                             <  1:4298244/140‑1 (MQ=255)
                         ccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGaa                                                                                                             >  1:2246625/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGAGAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATAGCAATACGCTTTTCGTCCGGGGCGTAACCGTTCTTGCCGATGGTGGCGGAc                                                                                                            >  2:3712643/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACTCGGTTCAGCAACCCACGGTCGCGTAGCGCCTGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                            >  1:1532988/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                            >  2:4193856/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                            >  2:1321053/1‑140 (MQ=255)
                               gCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCAc                                                                                                       >  1:1799378/1‑140 (MQ=255)
                               gCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCAc                                                                                                       >  2:5021730/1‑140 (MQ=255)
                                cTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACg                                                                                                      <  1:3042061/140‑1 (MQ=255)
                                  tttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                    <  2:1646672/140‑1 (MQ=255)
                                  tttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                    <  2:2772051/140‑1 (MQ=255)
                                    tAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTAcg                                                                                                  >  1:2158694/1‑140 (MQ=255)
                                    tAAATGCGAGCGGCGCGTAATATCCATCGACAGCATGCCGCGGTTCAGCAACCCACGGCCGCGGAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTAcg                                                                                                  >  2:3375428/1‑140 (MQ=255)
                                       aTGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACgcgc                                                                                               <  1:1645082/140‑1 (MQ=255)
                                          ggAGCGGCGCGCAATATCCATCGACAGCATAAAGCGGTTCAGCAACCCACGGTCGCTTAGGGCATGAAGCAGCGCAATACGCTTTTCGTCCCGGCAGTAACTGTTCTTGCCGCTGGAGTCGAACTGCACGTACGCGCCga                                                                                            >  2:1005054/1‑140 (MQ=255)
                                           gAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtgt                                                                                                                 <  1:1431055/118‑1 (MQ=255)
                                           gAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCgag                                                                                           >  2:600792/1‑140 (MQ=255)
                                              cggcgCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatc                                                                                        >  2:2192570/1‑140 (MQ=255)
                                                    gTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGatcat                                                                                  <  1:3297604/140‑1 (MQ=255)
                                                         atCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCa                                                                             <  1:3566028/140‑1 (MQ=255)
                                                           ccATCGACAGCATGACGCGGTTCAGCAACCCACGGACGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaa                                                                           <  2:3485152/140‑1 (MQ=255)
                                                            cATAGACGGCATGACGCGGTTCAGCAACCCACGGTGGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTCGTAACTGTTCGTGCCGATTGTGTCGAACTGAACGTACGCGCCGAGATCGATCATCTTCaaaa                                                                          <  2:950596/140‑1 (MQ=255)
                                                             aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                                         >  1:787310/1‑140 (MQ=255)
                                                             aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTGCAAAAt                                                                         >  2:3155423/1‑140 (MQ=255)
                                                              tCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATTAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAtg                                                                        >  1:414092/1‑140 (MQ=255)
                                                              tCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAtg                                                                        <  2:4363164/140‑1 (MQ=255)
                                                               cGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAtgt                                                                       >  1:1083166/1‑140 (MQ=255)
                                                                  cAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAtgttgt                                                                    >  2:5099383/1‑140 (MQ=255)
                                                                     cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCgg                                                                 >  1:1383030/1‑139 (MQ=255)
                                                                     cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGa                                                                 >  1:1984869/1‑140 (MQ=255)
                                                                     cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGa                                                                 >  1:3689793/1‑140 (MQ=255)
                                                                     cATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGa                                                                 >  1:3795729/1‑140 (MQ=255)
                                                                       tGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAgg                                                               >  1:2542068/1‑140 (MQ=255)
                                                                         aCGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                             >  1:1738573/1‑140 (MQ=255)
                                                                         aCGCGGTTCAGCAACCCACGGGCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTACCTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGtt                                                             <  2:4709024/140‑1 (MQ=255)
                                                                               ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCttt                                                       >  1:3816692/1‑140 (MQ=255)
                                                                               ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCttt                                                       >  2:4141867/1‑140 (MQ=255)
                                                                               ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCttt                                                       >  1:1230275/1‑140 (MQ=255)
                                                                               ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGCTGTCttt                                                       >  1:630857/1‑140 (MQ=255)
                                                                                        ccACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTTCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                              <  1:4663572/140‑1 (MQ=255)
                                                                                        ccACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                              <  1:4420387/140‑1 (MQ=255)
                                                                                         cACGGTCGCGTAGCGCATGAAGCATTTGAAGACGTTTTTCGTCCGGGTAGAAACTGTTTTTTCTGATGGTGTGGCGCTGCAGACGAGCGTTGAGATGGAGAATCTTCAGAGTGCTGTCTAGGTTGTGTTTCAGATCGGAg                                             >  2:3319214/1‑140 (MQ=255)
                                                                                         cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGcgg                                             >  2:1975048/1‑138 (MQ=255)
                                                                                         cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAg                                             >  2:1848625/1‑140 (MQ=255)
                                                                                         cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAg                                             >  2:2324375/1‑140 (MQ=255)
                                                                                         cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAg                                             >  1:246209/1‑140 (MQ=255)
                                                                                           cGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTg                                           <  2:4940100/140‑1 (MQ=255)
                                                                                            ggTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCtt                                                        >  1:2435779/1‑126 (MQ=255)
                                                                                             gTCGCGGGGCGCATCAAGCATCGCAATGCGGTTTTAGGCCGGGTAGGATCTGGTCGGGCGGGTGGTGTCGAGCTGGCGGTGCGCGCCGAGATCGATCATCTTCAGAATGTTGTCGAGGTTGTCGTTCAGATCGCAGTGAc                                         <  2:2576876/140‑1 (MQ=255)
                                                                                                 cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAAc                                     >  2:1934491/1‑140 (MQ=255)
                                                                                                  gTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACg                                    >  2:669210/1‑140 (MQ=255)
                                                                                                   tAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACgg                                   >  2:3525798/1‑140 (MQ=255)
                                                                                                      cgcATGTAGCATGGCACTACGCTTTTCGTCCGTGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGAACGTACGCGCCGAGATCGATCATCTTCAAAATGTGGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGa                                <  2:920608/140‑1 (MQ=255)
                                                                                                      cgcATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGa                                <  1:4221008/140‑1 (MQ=255)
                                                                                                      cgcATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGa                                <  1:2708475/140‑1 (MQ=255)
                                                                                                        cATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGGCGAGATCGATCATCTTCAAAATGTTCTCTAGGATGTCTTTCAGATCGCAGTGACCGACGGTGAcg                              >  1:1915342/1‑140 (MQ=255)
                                                                                                        cATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcg                              >  1:884326/1‑140 (MQ=255)
                                                                                                        cATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGAGCGATCATCTTCAAAATGTTGTGGAGGTTGGCTTTCAGAGCGCAGTGACGAACGGTGGcg                              >  1:2619670/1‑140 (MQ=255)
                                                                                                        cATGAAGCATCGCAATACGCTTTTCGTCCGGATAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcg                              >  2:3890802/1‑140 (MQ=255)
                                                                                                             aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                         <  2:1490034/140‑1 (MQ=255)
                                                                                                                aTCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAg                      >  1:2335449/1‑140 (MQ=255)
                                                                                                                aTCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAg                      >  1:1498271/1‑140 (MQ=255)
                                                                                                                 tCGCAATCCGCGTTTCGTCCGGGCAGTAACTGTTCTTGCCGATGGTGTGTAATTGCACGTACGAGCCGGGAGCGATCGTCTTCAAAATGGTGGCCCGGTTGGCTTTCAGGGCGCATTGACCAACGGTGGCGCGCGataaa                     >  2:801502/1‑136 (MQ=255)
                                                                                                                  cGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGAt                    >  1:3213980/1‑140 (MQ=255)
                                                                                                                      aTACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAAc                <  2:3642844/140‑1 (MQ=255)
                                                                                                                       tACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAAcc               >  2:1200904/1‑140 (MQ=255)
                                                                                                                       tACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAAcc               >  1:1027636/1‑140 (MQ=255)
                                                                                                                         cGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAAcccc             >  1:2973017/1‑140 (MQ=255)
                                                                                                                          gCTTTTCGTCCTGGTCGTAACTGTTCTTGCCGATCGCGTCGAACCGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTCGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCg            <  2:2539359/140‑1 (MQ=255)
                                                                                                                          gCTTTTCGTCCGGGTAGTAACTATTCGTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGCCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCg            <  2:4020262/140‑1 (MQ=255)
                                                                                                                            ttttCGTCCAGGTAGTAACTGTTCTTGCCGATGGGGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAGGTTGTCGAGGTTGGCTTTCAGATCGCAGTGACCAACGGTGACGTGCGAAAGATCAACCCCGTg          >  2:4299218/1‑140 (MQ=255)
                                                                                                                              ttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGGTCGATCATCTTCAAAATGTTGTCGAGGTTGTCTGTCAGATCGCAGTGACCCACGGTGACGCGCGAAAGATCAACCCCGTggg        >  2:2140621/1‑140 (MQ=255)
                                                                                                                              ttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTggg        >  1:2134079/1‑140 (MQ=255)
                                                                                                                               tCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGc       >  1:5025083/1‑140 (MQ=255)
                                                                                                                               tCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGAGCGCAGTGACTAACGGTGGCGCGCGAAAGATCAACCCCGTGGGc       >  2:181941/1‑140 (MQ=255)
                                                                                                                               tCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGATTGTCTTTCAGATCGCAGTGACCGACGGTGACGCGCGAAAGATCAACCCCGTGGGc       >  1:3647472/1‑140 (MQ=255)
                                                                                                                                    cGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTGGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGTa  >  1:575674/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TAGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGTA  >  NZ_CP025268/3508491‑3508762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑