Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,516,790 C→T K401K (AAG→AAA)  damX ← cell division protein DamX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,516,7900CT99.0% 1152.5 / NA 312K401K (AAG→AAA) damXcell division protein DamX
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (158/149);  minor base G (0/3);  total (160/152)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

GCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTAG  >  NZ_CP025268/3516655‑3516926
                                                                                                                                       |                                                                                                                                        
      cAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCct                                                                                                                                >  2:1028801/1‑140 (MQ=255)
       aGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCctc                                                                                                                               <  1:2242565/140‑1 (MQ=255)
           aaCTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCtcttct                                                                                                                           >  2:1121825/1‑140 (MQ=255)
              tCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTc                                                                                                                        >  1:4737071/1‑140 (MQ=255)
                      cGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTa                                                                                                                <  1:2696202/140‑1 (MQ=255)
                      cGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTa                                                                                                                <  2:1556617/140‑1 (MQ=255)
                        aTAAACCATTGCGGGAGATAACCTTGATTACTTCGGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTaca                                                                                                              >  1:4709703/1‑140 (MQ=255)
                        aTAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGGCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCTAAGCGTaca                                                                                                              >  1:497687/1‑140 (MQ=255)
                        aTAAACCATTGCGGGAGATAACCTTGAGTACTTCAGAGCGGCCTGTACCTGCCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACGTCTGCTGGCCACGTAGATACCGCCTTTTTCGCCTCTTCCTTCGCAGCGTaca                                                                                                              >  1:3652044/1‑140 (MQ=255)
                             ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  1:2921974/140‑1 (MQ=255)
                             ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  1:486898/140‑1 (MQ=255)
                                ttGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCTCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCCTACACTCCACaa                                                                                                      >  2:905766/1‑140 (MQ=255)
                                ttGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaa                                                                                                      >  2:581951/1‑140 (MQ=255)
                                ttGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAAGGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaa                                                                                                      >  2:2798798/1‑140 (MQ=255)
                                 tGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaaa                                                                                                     >  1:2068846/1‑140 (MQ=255)
                                 tGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaaa                                                                                                     <  1:3825762/140‑1 (MQ=255)
                                  gCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGGTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                                    >  2:3770810/1‑140 (MQ=255)
                                   cGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTATCGCCCACGGGTTTTTGGCCTGGACGTCTGCTGGCTATGTAGATCGCGCTGTTTTTGCCTCTTCTTTCGAACCGTACACGCCAgtgacc                                                                                                   >  2:3957248/1‑135 (MQ=255)
                                    gggAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGGTTTTGGCCTGGACCTCTGCTGGCAATGTAGATACCGCTTGTGTCGCCTCTTCTTTCGAAGCGGACACGCCAGAAACCa                                                                                                  <  1:2277813/140‑1 (MQ=255)
                                       agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  2:1415532/140‑1 (MQ=255)
                                       agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  2:1901599/140‑1 (MQ=255)
                                       agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  1:4093823/140‑1 (MQ=255)
                                       agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  1:655291/140‑1 (MQ=255)
                                       agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  1:3235011/140‑1 (MQ=255)
                                       agaTAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               <  1:2624693/140‑1 (MQ=255)
                                           aaCCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATa                                                                                           >  1:1286442/1‑140 (MQ=255)
                                            aCCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATAc                                                                                          <  1:4826583/140‑1 (MQ=255)
                                            aCCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATAc                                                                                          <  1:1920332/140‑1 (MQ=255)
                                              cTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                                        <  2:4480931/140‑1 (MQ=255)
                                              cTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                                        <  1:1069535/140‑1 (MQ=255)
                                                tGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACAACg                                                                                      >  2:4959111/1‑140 (MQ=255)
                                                  aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGGAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGc                                                                                    >  2:4938448/1‑140 (MQ=255)
                                                  aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGc                                                                                    >  2:1663483/1‑140 (MQ=255)
                                                  aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCCCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGc                                                                                    >  2:345162/1‑140 (MQ=255)
                                                   ttACTTCAGATCGGCCTGTCCCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  1:2530366/140‑1 (MQ=255)
                                                   ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  2:3811305/140‑1 (MQ=255)
                                                   ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  1:1624074/140‑1 (MQ=255)
                                                   ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  2:1194848/140‑1 (MQ=255)
                                                     aCTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGa                                                                                 <  1:751286/140‑1 (MQ=255)
                                                       ttCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGTGTTTTTGTCCTGGACCTCTGCTGGCAATGTAGATACCGCTTTTTTCGACTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGTCATACCACGGCTGAcc                                                                               >  2:4070837/1‑140 (MQ=255)
                                                       ttCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGAcc                                                                               >  1:3039816/1‑140 (MQ=255)
                                                           gATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTa                                                                           <  2:2169281/140‑1 (MQ=255)
                                                            aTCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCCATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCAttcc                                                                          >  2:2947370/1‑138 (MQ=255)
                                                            aTCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAc                                                                          >  1:329776/1‑140 (MQ=255)
                                                               ggCCTGTACCTGACGTAGCGGTTTAGTCAACGTGTTTTGTGACTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGCAGCGTCCACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                       <  2:4030013/140‑1 (MQ=255)
                                                               ggCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                       <  1:2757969/140‑1 (MQ=255)
                                                               ggCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                       <  2:244690/140‑1 (MQ=255)
                                                               ggCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgcg                                                                       <  1:4328404/140‑1 (MQ=255)
                                                                gCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgt                                                                      >  1:684048/1‑140 (MQ=255)
                                                                 ccTGTACCTGACGCAGCGGTTTCGCCCACTGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  2:3118223/140‑1 (MQ=255)
                                                                 ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCg                                                                                                                       <  1:2718063/90‑1 (MQ=255)
                                                                 ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCg                                                                                                                       >  2:2718176/1‑90 (MQ=255)
                                                                 ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:3132764/140‑1 (MQ=255)
                                                                 ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:3389063/140‑1 (MQ=255)
                                                                 ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:1684495/140‑1 (MQ=255)
                                                                 ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:790971/140‑1 (MQ=255)
                                                                 ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:4343488/140‑1 (MQ=255)
                                                                     tACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCCttt                                                                 >  2:4529142/1‑140 (MQ=255)
                                                                          gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAg                                                            >  1:3975925/1‑140 (MQ=255)
                                                                          gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGATTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGGTTCATAg                                                            >  2:70756/1‑140 (MQ=255)
                                                                           aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGa                                                                                               >  1:4932664/1‑104 (MQ=255)
                                                                           aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  2:3496408/130‑1 (MQ=255)
                                                                           aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           <  1:4664763/140‑1 (MQ=255)
                                                                           aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  2:4855621/1‑140 (MQ=255)
                                                                           aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:3874425/1‑140 (MQ=255)
                                                                           aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:4344232/1‑140 (MQ=255)
                                                                           aCGCAGCGGTTTCGCCCACGGCTTTTTGGCCTGCACATCTGCTGGCAATGTATATACCGCTTTTTTCGCTTCTTCTTTCGAAGGGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCCTAGa                                                           >  2:163713/1‑140 (MQ=255)
                                                                           aCGCAGCGGCTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTGTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           <  1:2326973/140‑1 (MQ=255)
                                                                               aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAAc                                                       >  2:2692364/1‑140 (MQ=255)
                                                                               aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAAc                                                       >  2:1727812/1‑140 (MQ=255)
                                                                               aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAAc                                                       >  2:4646737/1‑140 (MQ=255)
                                                                               aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAAc                                                       >  1:1569697/1‑140 (MQ=255)
                                                                               aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAAc                                                       >  1:3698202/1‑140 (MQ=255)
                                                                               aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTGCATAGACAAc                                                       >  2:686833/1‑140 (MQ=255)
                                                                               aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTGTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAAc                                                       >  2:2890894/1‑140 (MQ=255)
                                                                                gCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACg                                                      <  1:1417723/140‑1 (MQ=255)
                                                                                 cGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTAGACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGt                                                     >  1:4224691/1‑140 (MQ=255)
                                                                                 cGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGAAACCGCTTTTTTCGCCTCTTCTTGCGAAGCGTACAAGCCAGAAACCAGGAAATACCACGGCTGACCATTCCGCGTCGATTAATAGACAACGt                                                     >  2:4713294/1‑140 (MQ=255)
                                                                                    tttCGCACACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGt                                                  <  1:3753150/140‑1 (MQ=255)
                                                                                            aCGGGTTTTTGGCCTGTACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTCTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCCGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGa                                          <  1:2829256/140‑1 (MQ=255)
                                                                                                      ggCCTGGCCATCTCCTGGACATGTAGCTACCGCTTCTTTCGCCTCTTCTTTCGAACACTACACGCCAGCAACCACGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGACTCtctttct                                <  2:4014512/140‑1 (MQ=255)
                                                                                                         cTGGACATCTGCAGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCg                             <  2:3599432/140‑1 (MQ=255)
                                                                                                          tcgACATCTGCTGCCAATGTAGATACCGAATTTTTGGTCTCGTCGTTCGAACCGTACGCGGCAGAAACCAGGACATACCACGGCTGGACATTACGCGTCGCTTCATAGGAAACGGGCTTTTTCAGATTCGCTTTCTTCGc                            >  2:2037314/3‑140 (MQ=255)
                                                                                                          tGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGc                            >  1:4539388/1‑140 (MQ=255)
                                                                                                            gACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCGGGACATACCACGGCTGACCATTACGCGTCGTTTTATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGccc                          >  1:544160/1‑140 (MQ=255)
                                                                                                            gACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGccc                          >  1:3008997/1‑140 (MQ=255)
                                                                                                              cATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCaa                        <  2:2108419/140‑1 (MQ=255)
                                                                                                              cATCTGCTGGCAATGTAGACACCGCTCTTTTCGCCTCCTCTTTCGAAGCGTACACGCCACAACCCAGGACATACCACGGCTGACCATTACCCGTCGTTTCATAGACCACGTAGTTTTTCAGATTCGCTTTCTTCGCCCaa                        <  2:533484/140‑1 (MQ=255)
                                                                                                                      ggCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAg                >  1:1781646/1‑140 (MQ=255)
                                                                                                                       gCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg               <  1:3499315/140‑1 (MQ=255)
                                                                                                                       gCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg               <  1:889106/140‑1 (MQ=255)
                                                                                                                            gTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTc          <  2:3691973/140‑1 (MQ=255)
                                                                                                                              aGATACCGGTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCATCCGTTCAGGTTGTctt        >  2:3618714/1‑138 (MQ=255)
                                                                                                                              aGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGt        >  1:343523/1‑140 (MQ=255)
                                                                                                                               gATTCCGCGTTTTTCGCCTCTTCTTTCCAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  2:4199387/140‑1 (MQ=255)
                                                                                                                               gATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  2:4657619/140‑1 (MQ=255)
                                                                                                                               caTACCGCTTTTTTCGCCTCTTCTTTCCAAGCGTACACGCCAGCAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  2:2586546/139‑1 (MQ=255)
                                                                                                                                aTACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAg      >  1:2388548/1‑140 (MQ=255)
                                                                                                                                 tACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGt     >  1:3552590/1‑140 (MQ=255)
                                                                                                                                  aCCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGtt    >  1:1371640/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                        
GCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTAG  >  NZ_CP025268/3516655‑3516926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑