BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	353,643	A→G	629 (TAA→TAG)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	353,643	0	A	G	100.0%	1029.7 / NA	303	629 (TAA→TAG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (101/202); total (101/202)

Only 100 of 303 total aligned reads displayed.

CAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGC  >  NZ_CP025268/353506‑353778

cAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGt                                                                                                                                       >  2:1358224/1‑140 (MQ=255)

         aaaaCGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCgg                                                                                                                              <  1:1459373/140‑1 (MQ=255)

         aaaaCGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCgg                                                                                                                              <  1:3776352/140‑1 (MQ=255)

           aaCGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGAt                                                                                                                            <  1:4764674/140‑1 (MQ=255)

                   ccGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcgcg                                                                                                                    <  1:1163067/140‑1 (MQ=255)

                   ccGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcgcg                                                                                                                    <  2:4320166/140‑1 (MQ=255)

                   ccGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTcgcg                                                                                                                    <  1:4952799/140‑1 (MQ=255)

                       agcaaTGCTGCGTCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTCCTGATCCTGCGTCGTTGGATCCGATCCGCCAGGCGATGGAAGCGTAGGTTCTTGTCGGATGCGTCGCGCGGt                                                                                                                <  2:4141229/137‑1 (MQ=255)

                       aaaaaTGCTGCGTCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCGGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGCCGCGCGGt                                                                                                                <  2:3614333/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGATCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                <  2:4517614/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGTCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                <  2:4681061/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                <  2:3522102/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                <  1:696454/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                <  1:1016977/140‑1 (MQ=255)

                       aaaaaTGCTGCGCCGCACGATCCAGACGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                <  2:4636619/140‑1 (MQ=255)

                       aaaaaGGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGt                                                                                                                <  2:1985922/140‑1 (MQ=255)

                         aaaTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGc                                                                                                              <  1:2213869/140‑1 (MQ=255)

                           aTGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTA‑TGTCGGATGCACcgcgcgacgcatc                                                                                                           <  2:321496/140‑8 (MQ=255)

                            tgctgcGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCACCGCGCGACGCATc                                                                                                           <  2:4808258/140‑1 (MQ=255)

                              ctgcGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCg                                                                                                         <  2:5116388/140‑1 (MQ=255)

                                 ggccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  2:816622/139‑1 (MQ=255)

                                 gcccgcACGATCCAGGCGCTTTGCGAAGGACGAGATCCTGGAGATCTGACGCCCATTGATGATCCTTCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  2:2550160/138‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  1:5008027/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  1:1509667/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  1:2254313/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  1:1941744/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  1:25533/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  1:4172395/140‑1 (MQ=255)

                                 cgccgcACGATCCAGGCGATTTGCGAAGGACGCGATACTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                      <  1:2854326/140‑1 (MQ=255)

                                   ccgcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACt                                                                                                    <  2:1506911/140‑1 (MQ=255)

                                     gcACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACtgt                                                                                                  <  1:2492704/140‑1 (MQ=255)

                                          ctCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGcc                                                                                             <  2:2718914/139‑1 (MQ=255)

                                           tcaaGGCGATTTGCGAAGGACGCGATCCTGGAGAACGGACGACCATTGATGATCCTGCGTCGTTGGATCAGATACGCGAGGCGAAGGAAGAGTAGATTATTGTCGGGTGCGTCGCGCCGTGCAGCCGGAATTGTGGGctg                                                                                            >  2:4853903/4‑138 (MQ=255)

                                           tCCAGGCGTTTTGATAAGCACTGGGTCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGATGAGTCGGTTCTTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCg                                                                                            <  2:2221665/140‑1 (MQ=255)

                                              aGGCGATTTGCGACGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATg                                                                                         >  1:3848790/1‑140 (MQ=255)

                                              aGGCGATTTGCGAAGGACGCGATTCTGGAGATCTGACGACCATTGAGGATCCTGCGTCGATGGATCAGATCAGCCAGGCGATTGAAGAGTAGGTTATTGTAGGATGCGGCGCGCGGTGCATCCGGCAGTATGTGcgtaag                                                                                         >  2:2387039/1‑135 (MQ=255)

                                                gCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGcc                                                                                       >  1:2419415/1‑140 (MQ=255)

                                                    tttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                   >  2:3340236/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCt                                                                                      >  1:3042210/1‑136 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  2:188732/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  1:2687153/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  2:3463021/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  2:676525/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  2:3894255/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  1:662362/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  1:634065/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  2:3000305/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  1:3217607/1‑140 (MQ=255)

                                                     ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  >  1:4214714/1‑140 (MQ=255)

                                                     ttGCGAAGACCGCGCTCCCGGAGAGCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATg                                                                                  <  2:1563281/140‑1 (MQ=255)

                                                                        ggAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTAt                                                               <  2:2653624/140‑1 (MQ=255)

                                                                           gaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                            <  1:4042814/140‑1 (MQ=255)

                                                                           gaTCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCAt                                                            <  1:4088538/140‑1 (MQ=255)

                                                                              cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGGCGCTGACGCGTTTTATCATGcc                                                         >  1:1578209/1‑140 (MQ=255)

                                                                              cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                         >  2:3680248/1‑140 (MQ=255)

                                                                              cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                         >  1:2722760/1‑140 (MQ=255)

                                                                              cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                         >  1:2594194/1‑140 (MQ=255)

                                                                              cTGACGACCATTGATGATACTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGcc                                                         >  1:4444327/1‑140 (MQ=255)

                                                                                gacgacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTa                                                       <  1:305825/140‑1 (MQ=255)

                                                                                gacgacCATTGACGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCTGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTACCATGCCTa                                                       <  2:1412201/140‑1 (MQ=255)

                                                                                   gacCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACgg                                                    <  2:73896/140‑1 (MQ=255)

                                                                                       aTTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGTCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCt                                                <  2:4443750/140‑1 (MQ=255)

                                                                                        ttGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTg                                               <  1:1626707/140‑1 (MQ=255)

                                                                                        ttGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTg                                               >  1:3544300/1‑140 (MQ=255)

                                                                                               tCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTAt                                                               >  2:1998404/1‑117 (MQ=255)

                                                                                               tCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTa                                        >  1:3996634/1‑140 (MQ=255)

                                                                                                ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAg                                       >  2:964722/1‑140 (MQ=255)

                                                                                                ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAg                                       <  2:2965390/140‑1 (MQ=255)

                                                                                                ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAg                                       <  1:758320/140‑1 (MQ=255)

                                                                                                ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAg                                       <  1:549179/140‑1 (MQ=255)

                                                                                                ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCGACGGACCGGAACCGTAg                                       >  2:500134/1‑140 (MQ=255)

                                                                                                ccTGCGTCGATGGATCAGATCCGCCAGGCGATGCAAGAGTTGGGAATTGTCGGATTCGTAGAGCGGTGCATCCGGCACAGCGTGCACATGCCTGATGCGACGCTGAGGGGCTTGATCATGAGTAAGAACCCGAACCGTAg                                       >  2:1211010/1‑140 (MQ=255)

                                                                                                 cTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAgg                                      <  1:4899213/140‑1 (MQ=255)

                                                                                                  tGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGt                                     <  2:3068658/140‑1 (MQ=255)

                                                                                                   gCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATTCCTACGGACCTGAACCGTAGGTc                                    >  2:2129717/1‑140 (MQ=255)

                                                                                                    cgtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCg                                   >  2:1714130/1‑140 (MQ=255)

                                                                                                      tcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTGGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGGCGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGa                                 <  1:4340244/140‑1 (MQ=255)

                                                                                                         ttGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGTACTGTGTGCCGATGTCTGATGCGACGCTGACGCGTTTTATCATGCGTACGGACCTGAACCGTAGGTCGGATaa                              >  1:876559/1‑140 (MQ=255)

                                                                                                         ttGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATaa                              >  2:3030059/1‑140 (MQ=255)

                                                                                                         ttGGATCAGATCCGCCAGGCGATGGAAGAGTAGGGTATTGTAGGATGCGTCGCGGGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGGGTGTTATCATGCCTACGGACCTGAACCGGAGGTCGTATaa                              >  2:3055418/1‑140 (MQ=38)

                                                                                                           ggATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAgg                            <  2:903526/140‑1 (MQ=255)

                                                                                                              tCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGgcgc                         >  2:4898581/1‑140 (MQ=255)

                                                                                                               cAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCt                        <  1:4596029/140‑1 (MQ=255)

                                                                                                                   tCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTcgcg                    <  2:3848694/140‑1 (MQ=255)

                                                                                                                    ccGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGt                   <  1:3454228/140‑1 (MQ=255)

                                                                                                                     cGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTc                  <  1:3704365/140‑1 (MQ=255)

                                                                                                                           gCGATGGACGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATcc            >  2:880672/1‑140 (MQ=255)

                                                                                                                           gCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGAATAAGGCGCTCGCGTCGCATcc            >  2:2834073/1‑140 (MQ=255)

                                                                                                                            cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCg           >  1:3298115/1‑140 (MQ=255)

                                                                                                                            cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCg           <  1:5028697/140‑1 (MQ=255)

                                                                                                                            cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCg           <  1:2081470/140‑1 (MQ=255)

                                                                                                                            cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCg           <  1:1904793/140‑1 (MQ=255)

                                                                                                                            cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCg           <  2:406189/140‑1 (MQ=255)

                                                                                                                            cGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCg           <  2:1042984/140‑1 (MQ=255)

                                                                                                                             gATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATcagg          <  1:970735/140‑4 (MQ=255)

                                                                                                                               tGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca        >  1:2312990/1‑140 (MQ=255)

                                                                                                                               tGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca        >  2:3554546/1‑140 (MQ=255)

                                                                                                                                 gAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACAcc      <  1:4834824/140‑1 (MQ=255)

                                                                                                                                   agagTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGCCGCGTTTTATCATGCCTACGGACCTGACCCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCAt    <  2:2349871/140‑1 (MQ=255)

                                                                                                                                     agTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGc  >  1:2767186/1‑140 (MQ=255)

CAATTGCCAAAAACGCGATCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATGC  >  NZ_CP025268/353506‑353778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`