BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,644,586	A→G	G428G (GGT→GGC)	prlC ←	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,644,586	0	A	G	99.0%	943.8 / NA	290	G428G (GGT→GGC)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (126/160); minor base C (1/2); total (128/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

CGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAT  >  NZ_CP025268/3644450‑3644722

  tGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTAc                                                                                                                                     >  2:4620116/1‑140 (MQ=255)

  tGACCGAACTCGTGGAAGAGGGTGATCACTTCGGCGTGAGTGAACAGCGCCGGTTTAACATTTACCGGGCGGTTGAAGGGACAAGTCAAATACGCGACCGGATTTTGCAAAGAACCATGAGCTTTACGCCTCTGGCCTAc                                                                                                                                     >  2:706925/1‑140 (MQ=255)

   gACCGAACTCGTGGGAGAGGGAGAGCACTGCGTCGTGAGTGAGCAGCGCCGGTTTACCATTTCCCGGGCGGGTGAAGGTACAAGTCAAATACGCGACCGGGTTTTGCAAAGAACCATCAGCGTGACGCATCTGGCCAACg                                                                                                                                    >  2:189641/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTGCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:2938228/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:3120095/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  2:3347469/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    <  1:1429097/140‑1 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    <  1:1590656/140‑1 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  2:3721041/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:2853965/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:720205/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  2:4848560/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:399369/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTGTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGATTTACGCATCTGGCCTACg                                                                                                                                    >  2:730882/1‑140 (MQ=255)

   gACCGAAATCGTGGAAGAGGGGGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATGTACCGGGCGGTTGAAGTTACGAGTCAAATACGCGACCGGTTTTTGCAAAAAACCATCAGCGTTACGCATCTGGCCTACg                                                                                                                                    >  2:134091/1‑140 (MQ=255)

      ccaaCTCGTGCAACATGGTGATCACTTCGTCGTGAGTTACCACCGCCCGATTACCCCTTACCTCGCGGTTGCTATTACATGTCAACTACGCGACCGGCTTTTGCAAATCCCCATCAGCCTTACCTATCCGGCCTAAGCAg                                                                                                                                 <  2:3265645/138‑1 (MQ=255)

       gAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGt                                                                                                                                <  1:4730891/140‑1 (MQ=255)

        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACAGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTc                                                                                                                               >  1:2418893/1‑140 (MQ=255)

          cTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTcat                                                                                                                             <  2:3928025/140‑1 (MQ=255)

                      ggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGccc                                                                                                                 >  1:280632/1‑140 (MQ=255)

                       gTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGcccc                                                                                                                >  1:261036/1‑140 (MQ=255)

                       gTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGcccc                                                                                                                >  2:1610405/1‑140 (MQ=255)

                        tGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccg                                                                                                               >  2:1182907/1‑140 (MQ=255)

                           tCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCctgccg                                                                                                            >  2:4227299/1‑140 (MQ=255)

                               ttCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCt                                                                                                        >  1:4133937/1‑140 (MQ=255)

                               ttCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCt                                                                                                        >  2:600727/1‑140 (MQ=255)

                                tcgtcgTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCtt                                                                                                       >  1:3936745/1‑140 (MQ=255)

                                    cgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGttt                                                                                                   >  1:1816544/1‑140 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATCCGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  1:3658501/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  1:3347469/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  1:3438652/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  1:4927695/140‑1 (MQ=255)

                                       gagtgaCCAGCGCCGGTTTACCATGTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTCCGCATCTGGCGTACGCAGTCATCCGTCCTCGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:5035444/140‑1 (MQ=255)

                                       gagtgaCCAGCGCCGGTTTACCACTTACCGGGCGGTTGAAGTTACACGTCAAATACGCGACCGGTTCTTGCAAAGAACCCTCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:4765956/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTCCCATTTACCGGGCGGTTGAAGTTACAAGTCAAATCCGCGACCCGTTTTTGCAAAGACCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCGCGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:2149320/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGACGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATATGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  1:2051143/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:3539129/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:1556610/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTCACCGGGCGGTTGAAGTTTCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  1:4661081/140‑1 (MQ=255)

                                       gagtacaCAGCGACGGATTACCAATAAACGGGAGATTGAAGTTAAAAGACAAATACGCGCACGGTATTTGCAAAGACCCATCAGCTTCACGCATCTGGCCTCCGCAGTAAACCACCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:4394330/134‑1 (MQ=255)

                                        agtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCAc                                                                                               >  2:180779/1‑140 (MQ=255)

                                        agtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCAc                                                                                               >  1:1379079/1‑140 (MQ=255)

                                        agtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGGTACAAGTCAAATACTCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCAc                                                                                               >  1:3032335/1‑140 (MQ=255)

                                        agtgaACAGCGCCGGTTCACCATTTACCGGGCGGGTCAAGTTACAAGTCAGACACGCGCCCGGTTTTTGCAAAGAACCATCAACTGTACGGATCTGGCCTACGCAGTCATCCACCCACGCCCCTCCGCGCTTGTTTGCAc                                                                                               >  2:934490/1‑140 (MQ=255)

                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  2:4192978/140‑1 (MQ=255)

                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  1:2965789/140‑1 (MQ=255)

                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              >  2:655135/1‑140 (MQ=255)

                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  1:1194249/140‑1 (MQ=255)

                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  1:3721055/140‑1 (MQ=255)

                                         gtgaACAGCGCCGATTGACCATTTACCGGGCGATAAAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTAACGCATCAGGCCTACGCAGTCATGCATCCACTCCCCGCCGCGCGAGTTTTccgg                                                                                              >  2:2774696/1‑137 (MQ=255)

                                          tgaACCGCGCCGGTTTACCAGTGACCGGGCGGTCGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTTCCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACgg                                                                                             <  2:4502049/140‑1 (MQ=255)

                                          tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACgg                                                                                             <  2:3318651/140‑1 (MQ=255)

                                          tgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACgg                                                                                             <  2:1129426/140‑1 (MQ=255)

                                            aaCAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGc                                                                                           >  1:4282851/1‑140 (MQ=255)

                                             aCAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCa                                                                                          <  2:2420629/140‑1 (MQ=255)

                                               aGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCTTCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATa                                                                                        <  2:5019394/140‑1 (MQ=255)

                                               aGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATa                                                                                        <  2:3003597/140‑1 (MQ=255)

                                               aGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATa                                                                                        <  1:2396181/140‑1 (MQ=255)

                                                 cgcCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACa                                                                                      >  2:3991636/1‑140 (MQ=255)

                                                 cgcCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACa                                                                                      >  2:1414500/1‑140 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   >  1:553893/1‑140 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:348698/140‑1 (MQ=255)

                                                      gTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCg                                                                                 >  1:1700665/1‑140 (MQ=255)

                                                             aTTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGTTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGa                                                                          <  2:2076685/140‑1 (MQ=255)

                                                             aTTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGa                                                                          <  2:1060019/140‑1 (MQ=255)

                                                                tACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGACACGCCGCGCTTGTTTTCACGGGCATACAGCTCGAGGTAGAAGc                                                                       >  1:648801/1‑140 (MQ=255)

                                                                 aCCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCt                                                                      >  1:2536889/1‑140 (MQ=255)

                                                                  ccGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTa                                                                     <  1:2496764/140‑1 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  1:868813/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  1:1270960/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  1:1527898/1‑140 (MQ=255)

                                                                       ggcttGAAGTTACAAGTCACATACTCGACCGGTGTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCTCGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                <  2:790843/137‑1 (MQ=255)

                                                                       cGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                <  1:1039481/140‑1 (MQ=255)

                                                                       acgTTGAAGTCACCAGTCCACGGCGCGCCCGGTTTTTGCAAAGACCCAGCAGATTTACGCATCTGGCCTACGCAGTCATCCGGCCACGCCCCGCCGCGCTTGTTTTCCCGGGCATACAGATCGAGGTACAATCAACcgcg                                                                <  2:3640195/138‑1 (MQ=255)

                                                                        ggTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCgcgc                                                               <  1:2666957/140‑1 (MQ=255)

                                                                         gTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCa                                                              >  1:2177908/1‑140 (MQ=255)

                                                                         gTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCa                                                              >  1:4895487/1‑140 (MQ=255)

                                                                          ttGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAg                                                             >  2:3096482/1‑140 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:360842/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:1745392/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:3334823/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:436118/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCAGCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAGCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:3831298/140‑1 (MQ=255)

                                                                                    aaGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAttt                                                   >  2:4880427/1‑140 (MQ=255)

                                                                                    aaGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAttt                                                   <  2:3228064/140‑1 (MQ=255)

                                                                                            tACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                                           <  1:4491932/140‑1 (MQ=255)

                                                                                            tACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                                           <  1:100961/140‑1 (MQ=255)

                                                                                                      gTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaag                                 >  2:2257095/1‑140 (MQ=255)

                                                                                                      gTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaag                                 <  1:2073017/140‑1 (MQ=255)

                                                                                                                  aaCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATcc                     <  2:892337/140‑1 (MQ=255)

                                                                                                                   aCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCg                    <  1:3255154/140‑1 (MQ=255)

                                                                                                                        cAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGc               <  1:2269153/140‑1 (MQ=255)

                                                                                                                          gCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCa             <  1:1182907/140‑1 (MQ=255)

                                                                                                                          gCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACACTTCGAAGAAACGTACATCCGGATGCCa             <  2:3758982/140‑1 (MQ=255)

                                                                                                                            tttACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                <  1:918086/87‑1 (MQ=255)

                                                                                                                               aCGCATCTCGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACAt        <  2:2942530/140‑1 (MQ=255)

                                                                                                                                cGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATc       >  1:1858679/1‑140 (MQ=255)

                                                                                                                                cGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATc       >  1:57828/1‑140 (MQ=255)

                                                                                                                                cGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGGAGAAGCTACCGCGCAGTTCGTTATTTTCGTCACACAGTTCGAAGAAACGTACATACGGATGCCAGACATc       >  1:1478099/1‑140 (MQ=255)

                                                                                                                                  cATCTGGCCTCCGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCCGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCaa     <  2:2647401/140‑1 (MQ=255)

CGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAT  >  NZ_CP025268/3644450‑3644722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`