BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,752,014	A→G	287 (TAA→TAG) K3E (AAG→GAG)	sgbU → araD →	L‑ribulose‑5‑phosphate 3‑epimerase L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,752,014	0	A	G	99.2%	866.2 / NA	264	287 (TAA→TAG) K3E (AAG→GAG)	sgbU araD	L‑ribulose‑5‑phosphate 3‑epimerase L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (162/99); minor base T (2/0); total (165/99)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

CTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTA  >  NZ_CP025268/3751880‑3752150

cTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCaa                                                                                                                                     <  1:4710915/140‑1 (MQ=255)

 tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  1:3460763/1‑140 (MQ=255)

 tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  2:4146883/1‑140 (MQ=255)

 tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  2:4112270/1‑140 (MQ=255)

 tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAAc                                                                                                                                    >  2:3848964/1‑140 (MQ=255)

 tGCATAAACTGAACTATCGCGGGTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGATGGATTGAAGCGCGTATGCAGGAGGCTGGATTTAAATGTTAGAGCaaa                                                                                                                                    >  2:4263791/1‑139 (MQ=255)

  gCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACt                                                                                                                                   <  1:412555/140‑1 (MQ=255)

   cATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTg                                                                                                                                  >  2:691223/1‑140 (MQ=255)

   cATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTg                                                                                                                                  >  2:3899446/1‑140 (MQ=255)

   cATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTg                                                                                                                                  >  2:1200413/1‑140 (MQ=255)

      aaaCTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCG‑AAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAg                                                                                                                              <  2:1840123/140‑1 (MQ=255)

            aaCTAGCGCGGTTCTTTCCTGAGTGAGATGTGGACCGAAAAAGCCAAAGGGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGAc                                                                                                                         <  2:3626894/140‑1 (MQ=255)

             aCTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACg                                                                                                                        >  1:1091238/1‑140 (MQ=255)

                 tCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCt                                                                                                                    >  2:1141997/1‑140 (MQ=255)

                  cgcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTg                                                                                                                   >  2:1482980/1‑140 (MQ=255)

                      gttgttTCCTGATTGAGATGTGGACCG‑AAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTggcggc                                                                                                              <  2:2813428/140‑1 (MQ=255)

                      gTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTggcgg                                                                                                               >  1:1895790/1‑140 (MQ=255)

                           ttCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAAt                                                                                                          >  1:4899890/1‑140 (MQ=255)

                                 aTTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGgcg                                                                                                    >  2:4535877/1‑140 (MQ=255)

                                   tGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCt                                                                                                  >  1:4522663/1‑140 (MQ=255)

                                     agaTGTGGACCGAAAAAGCCACAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                                >  1:2602702/1‑140 (MQ=255)

                                     agaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                                >  2:879818/1‑140 (MQ=255)

                                      gaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTcc                                                                                               <  1:559077/140‑1 (MQ=255)

                                         gtgGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGc                                                                                            <  2:3419062/140‑1 (MQ=255)

                                          tgGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGATCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCt                                                                                           <  1:4357004/140‑1 (MQ=255)

                                          tgGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCt                                                                                           <  1:3004916/140‑1 (MQ=255)

                                          tgGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCt                                                                                           >  2:1572439/1‑140 (MQ=255)

                                            gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         <  2:4854794/140‑1 (MQ=255)

                                            gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         >  2:4738406/1‑140 (MQ=255)

                                              ccGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCAcc                                                                                       <  1:3251473/140‑1 (MQ=255)

                                                 aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    >  1:869123/1‑140 (MQ=255)

                                                 aaaaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATc                                                                                    >  1:2799297/1‑140 (MQ=255)

                                                   aaaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGCCGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTg                                                                                  >  1:2751985/1‑140 (MQ=255)

                                                    aaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTgg                                                                                 <  1:2277280/140‑1 (MQ=255)

                                                    aaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTgg                                                                                 <  1:4603918/140‑1 (MQ=255)

                                                     aGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGt                                                                                <  1:2690187/140‑1 (MQ=255)

                                                          aaGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGt                                                                           <  1:4975037/140‑1 (MQ=255)

                                                           agagCCGTTGCTGGAGATTATTCAGGCGCGGCGTTGGCTTGGACCGCGTCTGCAGGAGGCTGGCGTTATATGTTATAGCCACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCGCGCTCCCCATCTTGTTACGtg                                                                          >  2:4048138/1‑139 (MQ=255)

                                                           agagCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGtt                                                                          >  2:254823/1‑140 (MQ=255)

                                                            gagCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTc                                                                         <  1:3422450/140‑1 (MQ=255)

                                                              gCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCAc                                                                       <  1:3982309/140‑1 (MQ=255)

                                                               ccGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCAcc                                                                      <  1:691223/140‑1 (MQ=255)

                                                                cGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                     >  2:419473/1‑140 (MQ=255)

                                                                       ggAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCaa                                                              >  2:3292930/1‑140 (MQ=255)

                                                                        gagaTTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAAt                                                             >  1:4828064/1‑140 (MQ=255)

                                                                        gagaTTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAAt                                                             >  2:4299088/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAGTGTCAg                                                        >  2:3994089/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  1:3801372/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  2:2832794/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  2:3062235/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  1:238116/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  2:3315523/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  1:4205139/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                        >  1:2022107/1‑140 (MQ=255)

                                                                             tattCAGGCGCGGCGTTGGATGGAAGCGCGTATGCAGGAGCCTGGATGCAGATGTTAGAGCAGCTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCGCGCTCACCATCTGGTGACGTTCACCTGGGGCACTGTCAg                                                        >  2:2033464/1‑140 (MQ=255)

                                                                               ttCAGGCGCTTCGTGGGAGGGAAGGGCGTATGCAGGAGGCTGGGTTTATATGTTAGGGCAACTGAAAGCCGACGTGCGGGCGGCGAATCTGGCGCTTCCCGCTCACCGTCTGGTGACGTTCGCCTGGGGCAATGTCAgcg                                                      >  2:3157924/1‑140 (MQ=255)

                                                                               ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcg                                                      <  1:2614255/140‑1 (MQ=255)

                                                                                tCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcgc                                                     <  2:338822/140‑1 (MQ=255)

                                                                                tCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGCCGCTTCCAGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcgc                                                     <  2:449180/140‑1 (MQ=255)

                                                                                    gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTa                                                 >  2:2493765/1‑140 (MQ=255)

                                                                                    gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTa                                                 >  2:2746134/1‑140 (MQ=255)

                                                                                    gcgcGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTa                                                 >  2:4073689/1‑140 (MQ=255)

                                                                                     cgcgGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAg                                                >  1:1177774/1‑140 (MQ=255)

                                                                                           gTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGACAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGaaa                                          <  2:1489268/140‑1 (MQ=255)

                                                                                             tGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAAcg                                        <  2:512134/140‑1 (MQ=255)

                                                                                             gggATTGCAGCGCGTGTGACGGAGGCTGGAGAGATATATAAGAGAAACTGAAAGGCGAGGGGCTGGCGGCGATTCTAGCGAGCTCCGCGAACCATCTGGTGCCGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAAcg                                        <  2:655537/139‑1 (MQ=255)

                                                                                                  tGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCa                                   <  1:3901856/140‑1 (MQ=255)

                                                                                                    aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 <  1:1775530/140‑1 (MQ=255)

                                                                                                    aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 >  1:3188492/1‑140 (MQ=255)

                                                                                                    aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 >  1:790701/1‑140 (MQ=255)

                                                                                                    aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 >  1:2182589/1‑140 (MQ=255)

                                                                                                    aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 >  2:1119420/1‑140 (MQ=255)

                                                                                                    aaGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 <  1:89554/140‑1 (MQ=255)

                                                                                                    aaGCGCGTATGCAGAAGGCTGGATTTATATGTTAGAGCAACTGAGAGCCGACGTGCTGGCGGCGACTCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAat                                 <  1:3600793/140‑1 (MQ=255)

                                                                                                      gcgcGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                         >  1:13252/1‑82 (MQ=255)

                                                                                                        gcgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGGCGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                             <  1:933567/140‑1 (MQ=255)

                                                                                                        gcgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                             <  2:3458539/140‑1 (MQ=255)

                                                                                                         cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGa                                                                                                                                 >  1:148540/1‑39 (MQ=255)

                                                                                                         cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  1:4372326/1‑140 (MQ=255)

                                                                                                         cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  2:1856357/1‑140 (MQ=255)

                                                                                                         cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  1:69736/1‑140 (MQ=255)

                                                                                                         cgTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggatg                            >  1:2251407/1‑140 (MQ=255)

                                                                                                         cgTATGCAGGAGGCTGGATTTATATGTGAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACGCTCTGGTGACGTTCACCTGGGGCACTGTCAGCGCGGTAGACGAAGCTCGGCAatggatg                            >  2:2547409/1‑140 (MQ=255)

                                                                                                              gCAGGAGGCTGGATGTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTCGCTCTTCCCGCTCACCACCTGGTTACGTTCACCCGGGGCAACGTCAGCGCGGTACACGAGACGCGGCAATGGATCGTAAt                       >  2:3054463/1‑140 (MQ=255)

                                                                                                                  gaggCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCaaa                   <  2:737461/140‑1 (MQ=255)

                                                                                                                  gaggCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCaaa                   >  1:1366654/1‑140 (MQ=255)

                                                                                                                    ggCCGGAATTATATGTTCCAGCAACTGAAAGCCGACGTGCTGGCGGCGAAGCTGGCGCTTCCCGCTCACCATCTGGTGAGGTTAACCTGGGGCAATGTCAGCGCGGTAGACGACACGCGGCAATGCATGGTCAGCAAAcc                 >  2:421745/1‑140 (MQ=255)

                                                                                                                     gCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAAcat                >  2:1635610/1‑138 (MQ=255)

                                                                                                                     gCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCt                >  2:2099202/1‑140 (MQ=255)

                                                                                                                     gCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCGGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCt                >  2:2913746/1‑140 (MQ=255)

                                                                                                                         gATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCg            <  1:1987380/140‑1 (MQ=255)

                                                                                                                         gATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCg            <  2:581905/140‑1 (MQ=255)

                                                                                                                            ttATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCg         <  2:2137150/140‑1 (MQ=255)

                                                                                                                            ttATATGTGAGAGCAACTGAAAGCCGACGTGCGGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCg         <  2:2315400/140‑1 (MQ=255)

                                                                                                                              atatGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTc       >  1:3658014/1‑140 (MQ=255)

                                                                                                                              atatGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTc       >  1:4983221/1‑140 (MQ=255)

                                                                                                                              atatGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTAAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTc       >  2:4078629/1‑140 (MQ=255)

                                                                                                                              atatGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGCCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTc       <  2:4810398/140‑1 (MQ=255)

                                                                                                                                   ttAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTa  >  1:1695089/1‑140 (MQ=255)

                                                                                                                                   ttAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTa  >  1:4596223/1‑140 (MQ=255)

CTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGTA  >  NZ_CP025268/3751880‑3752150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`