BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,794,748	T→C	286 (TAA→TAG)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,794,748	0	T	C	98.4%	398.8 / ‑6.1	125	286 (TAA→TAG)	yibB	protein YibB
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (43/79); minor base A (1/1); total (45/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

AAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAG  >  NZ_CP025268/3794613‑3794883

aaaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAtt                                                                                                                                     >  2:2300085/1‑140 (MQ=255)

 aaaaCTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTAttt                                                                                                                                    >  2:1688987/1‑140 (MQ=255)

   aaCTATACAGTTTTGGCAGGGCATAAGGATGATTGATGTTACTGCAGTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTGTTTCGTATCTCAGTTGGTATCAAAACTCTACCCACTAATGGGGGGAGACTGAttgc                                                                                                                                   >  2:2545375/1‑138 (MQ=255)

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGTAAACCTATTTTCTTGa                                                                                                                              >  2:3910334/1‑140 (MQ=255)

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                                                              >  1:1793436/1‑140 (MQ=255)

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                                                              <  1:732594/140‑1 (MQ=255)

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                                                              <  1:3395743/140‑1 (MQ=255)

                tGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGGAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAAt                                                                                                                     >  2:4447590/1‑140 (MQ=255)

                       gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAgc                                                                                                              <  1:587726/140‑1 (MQ=255)

                       gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAgc                                                                                                              >  2:4124796/1‑140 (MQ=255)

                           aaTGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATGAGAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCt                                                                                                          >  1:4238783/1‑140 (MQ=255)

                                 gattgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAttt                                                                                                    >  2:819310/1‑140 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   <  1:4887338/140‑1 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   >  1:730825/1‑140 (MQ=255)

                                  attgatgTGACTGCGTGGACATAAGAAGCAAGAATGATGCTAAAGGATCAGT‑TTTCCTATCTCATTTGGTAGCAAGACGCTCCCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   <  2:4980968/140‑1 (MQ=255)

                                    tgattGTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCCGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTg                                                                                                 <  1:1160804/140‑1 (MQ=255)

                                           aCTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaa                                                                                          <  1:2944808/140‑1 (MQ=255)

                                           aCTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaa                                                                                          <  1:1560032/140‑1 (MQ=255)

                                            cTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAg                                                                                                               >  2:3659883/1‑118 (MQ=255)

                                            cTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAg                                                                                                               <  1:3659883/118‑1 (MQ=255)

                                                aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGACAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                     <  1:3007095/140‑1 (MQ=255)

                                                aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                     <  1:1683438/140‑1 (MQ=255)

                                                aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                     <  1:4876960/140‑1 (MQ=255)

                                                aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTAGCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                     <  2:51093/140‑1 (MQ=255)

                                                aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TATCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                     >  1:4403320/1‑140 (MQ=255)

                                                 tttatatAAAAAGAAAAAATGATTCCAAAGCATAATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTTATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGtt                                                                                    <  2:4959723/137‑1 (MQ=255)

                                                 ttGATATAAAAAGAAAAAATGATGCCAAATCACAATT‑TTTCATATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCTTAAAGTGtt                                                                                    <  2:294584/140‑1 (MQ=255)

                                                 ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTCGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGtt                                                                                    <  1:3653234/140‑1 (MQ=255)

                                                    atatAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACt                                                                                 >  1:2020062/1‑140 (MQ=255)

                                                    atatAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACt                                                                                 >  1:5057164/1‑140 (MQ=255)

                                                         aaaaGACAAAATGATGCCACAGCATCATT‑ATTCCTATCTCTGTTTGTATCGAAACGCTCCCCACTAATGGAGGGAAACCTATTGTCTTGCAAGGCAAATTCTCAGCGCCTGCATTTTTGCCCCTAACGTGTTACTCCTGa                                                                            <  2:116677/140‑1 (MQ=255)

                                                         aaaaGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGa                                                                            >  1:3487696/1‑140 (MQ=255)

                                                              aaaaaaTGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAagcag                                                                       >  1:4589540/1‑140 (MQ=255)

                                                                   atgatgCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGaaa                                                                  <  2:3365508/140‑1 (MQ=255)

                                                                     gatgCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACa                                                                >  1:4950363/1‑140 (MQ=255)

                                                                        gCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt                                                             >  2:220444/1‑140 (MQ=255)

                                                                        gCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAAt                                                             >  2:4456532/1‑140 (MQ=255)

                                                                                    tt‑tttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTc                                                 <  1:3258998/140‑1 (MQ=255)

                                                                                    tt‑tttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTc                                                 <  1:819310/140‑1 (MQ=255)

                                                                                     t‑tttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTcc                                                <  1:4185788/140‑1 (MQ=255)

                                                                                     t‑tttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTcc                                                <  1:463406/140‑1 (MQ=255)

                                                                                      tttctcTATCTCAGTTGGTATTCAAACGCTACCCACTAATCGAGGGAATCCTATTTTCTTGAAATGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTCAAGCAGCGAAACAACTCGCACCATTTTcc                                                <  2:2242569/135‑1 (MQ=255)

                                                                                       tttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCct                                               <  2:1377704/140‑1 (MQ=255)

                                                                                        ttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctc                                              <  1:5116001/140‑1 (MQ=255)

                                                                                         tCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctct                                             <  1:3044623/140‑1 (MQ=255)

                                                                                         tCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctct                                             <  1:3158199/140‑1 (MQ=255)

                                                                                         tCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctct                                             <  1:3158175/140‑1 (MQ=255)

                                                                                         tCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctct                                             <  2:1068403/140‑1 (MQ=255)

                                                                                           cTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTcc                                           <  1:378970/140‑1 (MQ=255)

                                                                                           cTATCTCAGTGCGTATCAAAACGGTACCCACTAAGTGAGGGAAACCGATTTTCTTGAAAGGAAAATGCTCAGCGCCTTAATTTTTGCCCCTAAAGTGTTACTCCTGAAGCGGCGAAACAAATCGAACCATTTTCCTCTcc                                           <  2:3231644/140‑1 (MQ=255)

                                                                                              tctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCa                                        <  2:1081871/140‑1 (MQ=255)

                                                                                               ctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                       <  1:418742/140‑1 (MQ=255)

                                                                                               ctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                       <  1:226196/140‑1 (MQ=255)

                                                                                                tcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGa                                      <  1:3480239/140‑1 (MQ=255)

                                                                                                tcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGa                                      <  2:3956037/140‑1 (MQ=255)

                                                                                                tcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGa                                      <  2:2969836/140‑1 (MQ=255)

                                                                                                tcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGa                                      >  1:1178701/1‑140 (MQ=255)

                                                                                                    ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  1:3450827/140‑1 (MQ=255)

                                                                                                    ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  2:410498/140‑1 (MQ=255)

                                                                                                    ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  1:2545375/140‑1 (MQ=255)

                                                                                                    ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  2:3693087/140‑1 (MQ=255)

                                                                                                    ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  >  2:2591049/1‑140 (MQ=255)

                                                                                                    ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  1:220730/140‑1 (MQ=255)

                                                                                                    ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  1:1724411/140‑1 (MQ=255)

                                                                                                    ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCGCCCCAGATAGt                                  <  1:766799/140‑1 (MQ=255)

                                                                                                    ttGGTAACAAAACGCTGCCCCCCAAGGGAGTGAAACCTATTTTCTTGAAAGGAAAATGCTCAGCGGCCGCGTTGTTGCCCCTAAAGTCTTACTCCTGCAGCAGCGAAAAAAATCACACCCTTATCCTCTCCCCAGATCGt                                  <  2:3568824/140‑1 (MQ=255)

                                                                                                      ggTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGttt                                <  2:411976/140‑1 (MQ=255)

                                                                                                        tATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAg                              >  1:3518645/1‑140 (MQ=255)

                                                                                                        tATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAg                              >  2:3270476/1‑140 (MQ=255)

                                                                                                         aTCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAgg                             >  2:907101/1‑140 (MQ=255)

                                                                                                              aaCGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGaa                        >  2:4215976/1‑140 (MQ=255)

                                                                                                               aCGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAag                       <  2:2844183/140‑1 (MQ=255)

                                                                                                               aCGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAag                       <  1:293287/140‑1 (MQ=255)

                                                                                                               aCGCTACCCACTAATGGAGGGAAACCCGGTTTCTTCAAAGGAAAATTCCCAGCGCCTGCATTCTTGCCCCTAAAGTGTTAATCCTAAAGCGGCGACAAAAAGCGAACCGTTTGCCTCTGCCCAGATAGTTTAGGTTGagg                       <  2:2301265/140‑3 (MQ=255)

                                                                                                                   tACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGAt                   <  1:2594293/140‑1 (MQ=255)

                                                                                                                       cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCCTTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  2:3518645/140‑1 (MQ=255)

                                                                                                                       cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  2:4939637/140‑1 (MQ=255)

                                                                                                                       cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  2:4368757/140‑1 (MQ=255)

                                                                                                                       cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  2:2196872/140‑1 (MQ=255)

                                                                                                                       cACTAATGGAGGGAAACATATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTAATCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  2:152268/140‑1 (MQ=255)

                                                                                                                          tAATGGAGGGAAACCTCTTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACATATCGAACCATTTTCCTCTCCACAGATAGTTTAGGTTGAAGAGATCGGGGCg            <  2:915385/140‑1 (MQ=255)

                                                                                                                          tAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCg            <  2:915483/140‑1 (MQ=255)

                                                                                                                            aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  2:3373904/140‑1 (MQ=255)

                                                                                                                            aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  2:2191184/140‑1 (MQ=255)

                                                                                                                            aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  2:1215961/140‑1 (MQ=255)

                                                                                                                            aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  2:4146564/140‑1 (MQ=255)

                                                                                                                            aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  1:3482425/140‑1 (MQ=255)

                                                                                                                            aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  1:3973755/140‑1 (MQ=255)

                                                                                                                            aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTCCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  2:3479297/140‑1 (MQ=255)

                                                                                                                             tGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGttt         >  1:2718803/1‑140 (MQ=255)

                                                                                                                               gAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGa                                      >  1:3364359/1‑109 (MQ=255)

                                                                                                                               gAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGa                                      <  2:3364359/109‑1 (MQ=255)

                                                                                                                               gAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGt       >  1:5035140/1‑140 (MQ=255)

                                                                                                                                aGGGAAACCTATTTTCTTGAGAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTa      >  1:2230507/1‑140 (MQ=255)

                                                                                                                                 gggAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTaa     <  2:3554669/140‑1 (MQ=255)

                                                                                                                                 gggAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTaa     <  1:61653/140‑1 (MQ=255)

                                                                                                                                  ggAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAAt    >  1:213427/1‑140 (MQ=255)

                                                                                                                                  ggAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAAt    >  1:510586/1‑140 (MQ=255)

                                                                                                                                  ggAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAAt    >  2:1872233/1‑140 (MQ=255)

                                                                                                                                    aaaCCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAg  >  1:544041/1‑140 (MQ=255)

AAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATT‑TTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAG  >  NZ_CP025268/3794613‑3794883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`