BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,915,245	A→G	C115R (TGT→CGT)	glmS ←	glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,915,245	0	A	G	100.0%	1565.5 / NA	468	C115R (TGT→CGT)	glmS	glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (269/199); total (269/199)

Only 100 of 469 total aligned reads displayed.

ACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAT  >  NZ_CP025268/3915109‑3915380

aCTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACgcgc                                                                                                                                      >  1:1002711/1‑140 (MQ=255)

 cTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCt                                                                                                                                     >  2:1942971/1‑140 (MQ=255)

 cTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCt                                                                                                                                     >  2:3168297/1‑140 (MQ=255)

  tGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCtt                                                                                                                                    >  2:695612/1‑140 (MQ=255)

   gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                   >  2:2322836/1‑140 (MQ=255)

   gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTAGAGCCACGCGCttt                                                                                                                                   >  2:4433669/1‑140 (MQ=255)

    tACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCtttt                                                                                                                                  >  2:1204184/1‑140 (MQ=255)

    tACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCtttt                                                                                                                                  >  1:2690662/1‑140 (MQ=255)

     aCCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTa                                                                                                                                 <  1:665833/140‑1 (MQ=255)

     aCCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTa                                                                                                                                 <  1:2554407/140‑1 (MQ=255)

     aCCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTa                                                                                                                                 <  1:565525/140‑1 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCTGTTTCAGAAAGGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:2893841/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATCGCCACGCGCTTTTAGctc                                                                                                                             >  2:3873837/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:3161071/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:4322292/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:4154641/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:3973383/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:1895815/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:2207874/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:1345494/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:3406654/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:1388807/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:281235/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:899909/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:633928/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:4592996/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:374682/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:97912/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCCCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:5982/1‑140 (MQ=255)

         tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCACGTTGATTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCAGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:3060455/1‑140 (MQ=255)

          aCGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctct                                                                                                                            >  1:453423/1‑140 (MQ=255)

            gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                          >  1:4809796/1‑140 (MQ=255)

            gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                          >  1:3229765/1‑140 (MQ=255)

            gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGGTCACCAGATGGGCAATCACTTGGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                          >  2:4396552/1‑140 (MQ=255)

              accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                        >  1:4394381/1‑140 (MQ=255)

              accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                        >  2:4182974/1‑140 (MQ=255)

              accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                        >  2:4853744/1‑140 (MQ=255)

                cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                      >  1:4210878/1‑140 (MQ=255)

                 acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCCGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCGGTTAGCTCTTCACGCa                                                                                                                     >  1:2940469/1‑140 (MQ=255)

                 acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                     <  2:1235928/140‑1 (MQ=255)

                  cGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAg                                                                                                                    <  2:2559982/140‑1 (MQ=255)

                     aGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCgg                                                                                                                 >  1:4892939/1‑140 (MQ=255)

                       cTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGtt                                                                                                               >  1:1912476/1‑140 (MQ=255)

                         gccggATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACGTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCa                                                                                                             <  1:1022038/137‑1 (MQ=255)

                         gCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCa                                                                                                             <  2:3277822/140‑1 (MQ=255)

                          cGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCAt                                                                                                            >  2:866/1‑140 (MQ=255)

                          cGGGATAGCACGCAGAACGACGACACGCAGAGTCCCGCCTTGTTTCAGCTCCAAGTTCACCAGAAGAGCAATCACAACGGTGTCGGTGTCAGAAACGGAGGTAGAACCACGAGCTTATAGCTATGCACGcggcggatact                                                                                                            >  2:1739219/1‑135 (MQ=255)

                                    cGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgat                                                                                                  >  1:3555432/1‑140 (MQ=255)

                                      cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                <  2:2463765/140‑1 (MQ=255)

                                      cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                <  1:1734047/140‑1 (MQ=255)

                                       aGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatgat                                                                                               >  2:3667010/1‑140 (MQ=255)

                                        gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              <  2:1681009/140‑1 (MQ=255)

                                        gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              <  1:2315826/140‑1 (MQ=255)

                                        gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              <  1:3206324/140‑1 (MQ=255)

                                              ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                        <  1:5096609/140‑1 (MQ=255)

                                              ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                        >  2:5052131/1‑140 (MQ=255)

                                              ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTAGAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                        >  2:1973028/1‑140 (MQ=255)

                                              ccTCACGCACAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACCAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTAATGGTTTTCGATGATGCCGTTa                                                                                        <  2:2061118/140‑1 (MQ=255)

                                                ccACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATg                                                                                      <  1:2423433/139‑1 (MQ=255)

                                                  aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                    <  2:2782457/140‑1 (MQ=255)

                                                  aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                    <  1:1970570/140‑1 (MQ=255)

                                                   cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGCTATGcac                                                                                   >  1:3647611/1‑140 (MQ=255)

                                                   cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGGTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcac                                                                                   >  2:2647446/1‑140 (MQ=255)

                                                      agagTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccac                                                                                >  2:526702/1‑140 (MQ=255)

                                                        agTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacca                                                                              >  1:1116077/1‑140 (MQ=255)

                                                        agTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcaccacca                                                                              >  1:4732536/1‑140 (MQ=255)

                                                          tCCCGCCTTGTTTCCGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            <  2:1440246/140‑1 (MQ=255)

                                                            ccGCCTTGTTTCAGCTCCCAGTTCCCCAGATGGGCAAGCACTTCGGTGTCGGTTTCAGAAGCGAAGGTATAGCCACGCGCTTTTAGGTCTTGACGCAGCGGTTCATGGTTTTCGATGATGCCGTTAGGCACCACCACAAt                                                                          <  2:473517/140‑1 (MQ=255)

                                                            ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGc                                                                                             <  2:293304/121‑1 (MQ=255)

                                                            ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                          <  1:2352848/140‑1 (MQ=255)

                                                            ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                          >  2:1526816/1‑140 (MQ=255)

                                                                    tttCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAg                                                                  >  1:4611922/1‑140 (MQ=255)

                                                                         gCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACa                                                             >  1:3720300/1‑140 (MQ=255)

                                                                               aGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGa                                                       <  2:4263458/140‑1 (MQ=255)

                                                                                      cAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAt                                                >  2:1608935/1‑140 (MQ=255)

                                                                                       aGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAtt                                               <  1:3042027/140‑1 (MQ=255)

                                                                                        gATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTc                                              <  2:4824773/140‑1 (MQ=255)

                                                                                        gATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTc                                              <  2:2577484/140‑1 (MQ=255)

                                                                                         aTGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCa                                             >  1:1529321/1‑140 (MQ=255)

                                                                                           gggCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACt                                           >  1:4846639/1‑140 (MQ=255)

                                                                                              cAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGAGGCGCATTCACTTCt                                        >  2:1108752/1‑140 (MQ=255)

                                                                                               aaTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTg                                       <  2:4316463/140‑1 (MQ=255)

                                                                                               aaTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTg                                       <  1:768142/140‑1 (MQ=255)

                                                                                                aTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGa                                      >  2:3935300/1‑140 (MQ=255)

                                                                                                aTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGa                                      <  1:3077263/140‑1 (MQ=255)

                                                                                                aTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGa                                      <  1:5070703/140‑1 (MQ=255)

                                                                                                    cTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGt                                  <  1:1222418/140‑1 (MQ=255)

                                                                                                       cGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCg                                                                                           <  1:3951791/80‑1 (MQ=255)

                                                                                                       cGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCa                               >  2:1056477/1‑140 (MQ=255)

                                                                                                           gtCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGAATGCGCATTCACTTCTGAAGGTTCACCGt                           >  2:1812177/1‑140 (MQ=255)

                                                                                                             cGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTgg                         <  1:281542/140‑1 (MQ=255)

                                                                                                              ggTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACGTCTGAAGGTTCACCTTggg                        >  1:2214249/1‑140 (MQ=255)

                                                                                                               gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGt                       >  1:2064908/1‑140 (MQ=255)

                                                                                                               gTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGt                       <  2:4695672/140‑1 (MQ=255)

                                                                                                                      aaaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGCTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAg                <  2:4094874/140‑1 (MQ=255)

                                                                                                                           aaGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACGGTGGGTCGCCCAGCGAgt           <  2:4464957/140‑1 (MQ=255)

                                                                                                                           aaGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgt           <  2:3341196/140‑1 (MQ=255)

                                                                                                                                ataGCCAAGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGAGTCGCCCAGCGAGTGTGAg      <  2:3584555/140‑1 (MQ=255)

                                                                                                                                 taGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGc     <  1:3895533/140‑1 (MQ=255)

                                                                                                                                    ccACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAt  <  2:3973383/140‑1 (MQ=255)

ACTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAT  >  NZ_CP025268/3915109‑3915380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`