BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,943,931	A→G	noncoding (205/1542 nt)	CXP41_RS20515 →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,943,931	0	A	G	97.9%	741.9 / 1.4	332	noncoding (205/1542 nt)	CXP41_RS20515	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (167/157); total (169/163)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

TAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGA‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTC  >  NZ_CP025268/3943796‑3944067

tAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCtt                                                                                                                                      <  1:377382/140‑1 (MQ=14)

        aaCAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCctc                                                                                                                              >  1:4637212/1‑140 (MQ=18)

        aaCAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCctc                                                                                                                              >  1:1879879/1‑140 (MQ=18)

        aaCAGCTTGCTGTTTCGCTGACGAGTCGCGGACGGGTGAGTAATGTCTAGGAACCTGCCTCATGGAGGGGGATACCTACTGGAAACGGTAGCTGATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCctc                                                                                                                              <  2:1418106/140‑1 (MQ=11)

          cAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCtt                                                                                                                            <  2:1350384/140‑1 (MQ=18)

          cAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCtt                                                                                                                            <  2:2044480/140‑1 (MQ=18)

          cAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCtt                                                                                                                            <  2:2715992/140‑1 (MQ=18)

              ttGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCa                                                                                                                        >  1:1428164/1‑140 (MQ=18)

                    tttCGCTGACGAGTGTCCGACGGGTGAGTAATCTCTGTGACACTGCCTGATGGAGGGCGATAACTTCTGGCAACGGTAGATAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGac                                                                                                                  <  2:4245564/140‑2 (MQ=11)

                    tttCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGAt                                                                                                                  <  2:4637212/140‑1 (MQ=37)

                       cGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGAtgtg                                                                                                               >  2:850209/1‑140 (MQ=18)

                       cGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGAAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGAtgtg                                                                                                               <  1:1787465/140‑1 (MQ=16)

                       cGCTGACGAGTGGCGGACGGGTGAGGAATGTCTGGGAAACTGCCTGATGGCGGGGGATAACTACGGGAAACGGTAGCTAATACCGCATAACGTCTCAAGACCAAAGAGGGGGG‑ACTTGGGGCGTCTTGCCATCAGAtggg                                                                                                               >  2:300613/1‑138 (MQ=11)

                         cTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGTCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGcc                                                                                                             <  2:3044351/140‑1 (MQ=16)

                         cTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGcc                                                                                                             <  2:3863796/140‑1 (MQ=18)

                             cGAGTGGCGGACGGGTGAGTAATGTATGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGa                                                                                                         <  1:3150883/140‑1 (MQ=18)

                                  ggCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGa                                                                                                    <  1:3712390/140‑1 (MQ=18)

                                   gCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAt                                                                                                   >  2:3267573/1‑140 (MQ=18)

                                   gCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAt                                                                                                   <  1:1980304/140‑1 (MQ=18)

                                   gCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAt                                                                                                   <  1:1299784/140‑1 (MQ=18)

                                      gACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAg                                                                                                >  1:2161023/1‑140 (MQ=18)

                                         gggTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCta                                                                                             >  2:1868096/1‑140 (MQ=18)

                                          ggTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtag                                                                                            <  2:2184557/140‑1 (MQ=18)

                                           gTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtagt                                                                                           >  2:1961409/1‑140 (MQ=18)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAgg                                                                                        <  2:3690107/140‑1 (MQ=18)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAgg                                                                                        >  1:3672488/1‑140 (MQ=18)

                                               gTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGt                                                                                       <  1:4185932/140‑1 (MQ=17)

                                               gTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCGTCGGGCCTCTTGCGATCAGATGTGCCCAGATGGGATTAGCTAGTAGGt                                                                                       <  2:4381207/140‑1 (MQ=16)

                                                    gTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGt                                                                                  <  1:1392739/140‑1 (MQ=18)

                                                    gTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGt                                                                                  <  2:4942078/140‑1 (MQ=18)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 >  1:48423/1‑140 (MQ=18)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 >  1:663545/1‑140 (MQ=18)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 <  1:1360378/140‑1 (MQ=18)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 >  2:1692358/1‑140 (MQ=17)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 >  1:2934402/1‑140 (MQ=17)

                                                         ggAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGAGGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACgg                                                                             >  2:2281109/1‑140 (MQ=12)

                                                          gAAACTGCCTGAGGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGc                                                                            >  2:1179209/1‑140 (MQ=17)

                                                          gAAACTGCATTATGGAGGGGGATAACTACTGGAAACGGTAGCTAACACCGCATAACATCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATAGGATTAGCTAGTAGGTGGGGTAACGGc                                                                            <  2:3753113/140‑1 (MQ=11)

                                                              cTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAc                                                                        >  2:2591863/1‑140 (MQ=18)

                                                               tGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAcc                                                                       >  1:819896/1‑140 (MQ=18)

                                                               tGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAcc                                                                       >  1:4414104/1‑140 (MQ=18)

                                                               tGCCTGACGGAGGGGGATAACCACTCGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAGGAGGGGGG‑CCTTCGGTCCTCTTGCCACCAGATATGCCCAGATGGGATCAGCTAGCAGGTGGGGTCACGGCTCAcc                                                                       >  2:5113641/1‑140 (MQ=11)

                                                                      tGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGA‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcga                                                                <  1:4158171/140‑1 (MQ=33)

                                                                       ggAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcgac                                                               <  2:5019386/140‑1 (MQ=18)

                                                                           ggggATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATc                                                           >  2:2311092/1‑140 (MQ=18)

                                                                           ggggATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATc                                                           <  1:3328042/140‑1 (MQ=18)

                                                                           ggggATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGAGGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATc                                                           >  2:1285098/1‑140 (MQ=18)

                                                                               aTAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTa                                                       >  2:619653/1‑140 (MQ=18)

                                                                               aTAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTa                                                       >  1:1177394/1‑140 (MQ=17)

                                                                                tAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAg                                                      <  1:3172038/140‑1 (MQ=18)

                                                                                   ctactGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTg                                                   >  2:4812749/1‑140 (MQ=18)

                                                                                     actGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAt                                                                                                   >  1:3661423/1‑90 (MQ=25)

                                                                                       tGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCt                                               >  1:3886245/1‑140 (MQ=18)

                                                                                         gAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACCGCTCACCTAGGCGACGATCCCTAGCTGGACTgc                                             >  1:2453251/1‑139 (MQ=12)

                                                                                            aCGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGagag                                          <  1:1114819/140‑1 (MQ=18)

                                                                                            aCGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGagag                                          <  1:4085326/140‑1 (MQ=18)

                                                                                            aCGGTAGATAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTGCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGagag                                          >  2:2818867/1‑140 (MQ=12)

                                                                                              ggtcccTAATACCCCCGCACGCCGCAAGACCAAACAGGGGGA‑CCCTCTGGCTTCTGGGCATCAGATGTGCCCAGATGGGATTAGCAAGTAGGTGGGGTAAGGTCTCCCCTACGCGACGATCCCTAGCTAGTCTGACAGGa                                        <  2:2382985/135‑1 (MQ=2)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        >  2:4445021/1‑140 (MQ=18)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        >  1:1035879/1‑140 (MQ=18)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGGGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        >  1:1537473/1‑140 (MQ=18)

                                                                                                  gCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGAc                                    <  1:1271380/140‑1 (MQ=17)

                                                                                                      aTACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGc                                <  1:265196/140‑1 (MQ=18)

                                                                                                      aTACCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGc                                <  1:5116679/140‑1 (MQ=18)

                                                                                                        aCCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCca                              <  1:2712906/140‑1 (MQ=18)

                                                                                                        aCCGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCca                              >  1:4529523/1‑140 (MQ=18)

                                                                                                          cGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCcaca                            >  1:5041977/1‑140 (MQ=18)

                                                                                                          cGCATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCcaca                            >  1:3726813/1‑140 (MQ=18)

                                                                                                          cGCAGAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCcaca                            >  2:4723309/1‑140 (MQ=16)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  1:1016958/1‑140 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  1:3043349/1‑140 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  1:2056640/1‑140 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  1:2310696/1‑140 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGGAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  2:4187181/1‑140 (MQ=12)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATAGGATTAGCGAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCGGCCACACt                          >  2:2445866/1‑140 (MQ=255)

                                                                                                             aTAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTg                         >  1:164552/1‑140 (MQ=18)

                                                                                                              tAACGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTgg                        <  1:1669594/140‑1 (MQ=18)

                                                                                                                 cGTCGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAAc                     >  1:84376/1‑140 (MQ=18)

                                                                                                                    cGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTga                  >  1:58984/1‑140 (MQ=18)

                                                                                                                    cGCAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGGGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTga                  >  1:4372321/1‑140 (MQ=16)

                                                                                                                      cAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCGAGCCGGTCTGCGAGGATGACCAGCCACACTGGAACTgaga                >  2:4614885/1‑140 (MQ=255)

                                                                                                                      cAAGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGCGGGGTAACGGCTCACCTAGGCGACGCTCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTgaga                >  2:530512/1‑140 (MQ=12)

                                                                                                                       aaGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGac               <  2:3963965/140‑1 (MQ=18)

                                                                                                                       aaGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGac               <  2:752082/140‑1 (MQ=18)

                                                                                                                       aaGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGac               <  1:3850589/140‑1 (MQ=18)

                                                                                                                        aGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  2:2084359/140‑1 (MQ=17)

                                                                                                                        aGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  1:2591863/140‑1 (MQ=18)

                                                                                                                        aGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  2:1578756/140‑1 (MQ=18)

                                                                                                                        aGACCAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  1:2788863/140‑1 (MQ=18)

                                                                                                                        aGACCAAAGAGGGGGG‑CCATCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAACTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  2:3649220/140‑1 (MQ=16)

                                                                                                                          aCCAAAGGGGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGacac             <  2:1443396/140‑1 (MQ=255)

                                                                                                                            cAAAGAGGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGt          <  1:4979783/140‑1 (MQ=18)

                                                                                                                             acagagGGGGG‑CCTGCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACCCTGGAACTGAGACACGGTc         <  2:3135522/138‑1 (MQ=255)

                                                                                                                               agagGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCa       <  1:3512413/140‑1 (MQ=18)

                                                                                                                               agagGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCa       <  2:41219/140‑1 (MQ=17)

                                                                                                                               agagGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCa       <  2:3615611/140‑1 (MQ=18)

                                                                                                                                gagGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAg      <  1:948476/140‑1 (MQ=17)

                                                                                                                                 agGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGa     <  2:342804/140‑1 (MQ=18)

                                                                                                                                 agGGGGG‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGa     <  1:322053/140‑1 (MQ=17)

                                                                                                                                  gggggg‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGAc    <  2:1016958/140‑1 (MQ=17)

TAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGA‑CCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTC  >  NZ_CP025268/3943796‑3944067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`