BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,119,045	A→G	C34R (TGC→CGC)	glpK ←	glycerol kinase GlpK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,119,045	0	A	G	99.7%	1234.8 / NA	369	C34R (TGC→CGC)	glpK	glycerol kinase GlpK
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (154/213); total (154/215)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 369 total aligned reads displayed.

CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGC  >  NZ_CP025268/4118909‑4119178

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGa                                                                                                                                 <  1:1033424/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGa                                                                                                                                 <  1:3532060/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGa                                                                                                                                 >  2:4309708/1‑140 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGa                                                                                                                                 <  1:3855304/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGa                                                                                                                                 <  2:3126380/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGa                                                                                                                                 <  2:116871/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGa                                                                                                                                 <  1:2327324/140‑1 (MQ=255)

   aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTATACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCCTTGGGTCGTGTTCTACGCAACCGGGGTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGa                                                                                                                                 >  2:1083719/1‑140 (MQ=255)

     aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGaca                                                                                                                               <  1:3351441/140‑1 (MQ=255)

     aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGaca                                                                                                                               <  2:3864230/140‑1 (MQ=255)

     aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGaca                                                                                                                               <  1:3923025/140‑1 (MQ=255)

     aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGaca                                                                                                                               <  2:3670558/140‑1 (MQ=255)

     aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGaca                                                                                                                               <  2:1249961/140‑1 (MQ=255)

      gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGacac                                                                                                                              <  2:1601109/140‑1 (MQ=255)

       cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACg                                                                                                                             <  1:926600/140‑1 (MQ=255)

          cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTa                                                                                                                          >  1:178399/1‑140 (MQ=255)

          cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTa                                                                                                                          <  1:1668833/140‑1 (MQ=255)

          cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTa                                                                                                                          >  1:3453466/1‑140 (MQ=255)

            aTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAAt                                                                                                                        <  2:2520987/140‑1 (MQ=255)

                 aTCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGatat                                                                                                                   <  1:3435370/140‑1 (MQ=255)

                           atatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTa                                                                                                                          >  1:4695277/1‑123 (MQ=255)

                           atatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATATCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTg                                                                                                         >  2:348965/1‑140 (MQ=255)

                            tatCGGCTTTCGCCAGCCCTTCTACCAGCGTGGAGCTTTGGGTCGCCCAGATTTCCATTGGGTCGTATTCTGCCCATCCAGGTTTTGGGTAGACTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  2:1005496/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  1:1127736/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  2:2039697/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  1:555971/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  2:4692559/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  2:4994883/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  1:3593908/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  1:2379883/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGACATCGTGa                                                                                                        <  1:4724640/140‑1 (MQ=255)

                            tatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATATCCGTTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  1:3641042/140‑1 (MQ=255)

                                ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCa                                                                                                    >  2:2610331/1‑140 (MQ=255)

                                ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGGCGTGTTCTACCCAACGTGGTTGTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCa                                                                                                    >  2:3643173/1‑140 (MQ=255)

                                     tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        <  2:4864259/131‑1 (MQ=255)

                                     tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGa                                                                                                        >  1:4864259/1‑131 (MQ=255)

                                     tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACg                                                                                               >  1:2230582/1‑140 (MQ=255)

                                       gCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGAc                                                                                             <  2:3696002/140‑1 (MQ=255)

                                       gCCAGCACTTCTACCAGCGTCGAGCTTGCGGTGCCCCATATTCCCATTCGGATGTGTTCTCCCCAACCAGGTTTTGAGGAGAATTGCGC‑AAAGTCGAGCGGCGACTAGCTACGCATATTGCCAGGGTGATCCCTTACCAc                                                                                             <  2:2997583/140‑1 (MQ=255)

                                           gCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcg                                                                                         <  2:2792593/140‑1 (MQ=255)

                                             aCTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACACAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                                                                                       <  2:1353439/140‑1 (MQ=255)

                                                  tACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                  <  1:3129733/140‑1 (MQ=255)

                                                  tACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                  <  2:4151905/140‑1 (MQ=255)

                                                  tACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                  <  1:181250/140‑1 (MQ=255)

                                                  tACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                  <  1:1581968/140‑1 (MQ=255)

                                                  tACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGc                                                                                  <  1:132416/140‑1 (MQ=255)

                                                      aGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggt                                                                              <  1:2463133/140‑1 (MQ=255)

                                                       gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                             <  1:3793105/140‑1 (MQ=255)

                                                         gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAggtggtggt                                                                           >  1:119876/1‑140 (MQ=255)

                                                         gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTggtggt                                                                           >  1:2088439/1‑140 (MQ=255)

                                                          tGGAGCTTTGGGTGGCCCAGATTACCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACCCGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGgtggtg                                                                          <  2:1473910/140‑1 (MQ=255)

                                                            gAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGcc                                                                        <  1:3299262/140‑1 (MQ=255)

                                                            gAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGACCCATTACGACCGCGCGGGAGCTGGTGGTGcc                                                                        <  2:2661983/140‑1 (MQ=255)

                                                             aGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACACAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGccc                                                                       <  1:657042/140‑1 (MQ=255)

                                                                tttGGGTGGCCCAGTTTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTgg                                                                    <  1:3868502/140‑1 (MQ=255)

                                                                       ggCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg                                                             <  2:646771/140‑1 (MQ=255)

                                                                       ggCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg                                                             <  2:3188867/140‑1 (MQ=255)

                                                                         cccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                           >  1:3270072/1‑140 (MQ=255)

                                                                         cccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                           >  1:5035153/1‑140 (MQ=255)

                                                                                   cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtt                                                 >  1:800987/1‑140 (MQ=255)

                                                                                        ggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttttttt                                            >  1:1388529/1‑140 (MQ=255)

                                                                                          tCGTGTTCTACCCAACCTGGTTTTGGGTAGCTTGGCTC‑AAATTCGCGCTGCTACACGCTAATGATATTGGCATCGTGATCCATAACGACCGCACGGGAGCTGGTGGTGCACTGGTCGAGCGCACCGATATAATTTATTTc                                          >  2:2328173/1‑140 (MQ=255)

                                                                                          tCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTCTTTTTc                                          >  2:4949055/1‑140 (MQ=255)

                                                                                            gtgtTCTACCCAACCTGGTTTTGCGTAGATTTGCTC‑CAACTCGCACTGCGACACGCTAATGATATTGGCATCGTAATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCACCGATATATTTTTTTTCAg                                        <  2:3075446/140‑1 (MQ=255)

                                                                                             tgtTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGAGATATTTTTTTTCAGt                                       >  2:774535/1‑140 (MQ=255)

                                                                                               ttCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCa                                     >  1:4649037/1‑140 (MQ=255)

                                                                                                 gtACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGTCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATg                                   <  1:4514017/139‑1 (MQ=255)

                                                                                                    cccAACCTGGTTTTGGGTGGATTTGCTA‑AAATTCGCGCTGCGACACGCTAAGGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCAGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTAAGGCATGttt                                >  1:2668581/1‑140 (MQ=255)

                                                                                                    cccAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGttt                                >  2:1897408/1‑140 (MQ=255)

                                                                                                    cccAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTATTTTTCAGTCATGttt                                >  1:2836854/1‑140 (MQ=255)

                                                                                                      cAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                              <  2:4078556/140‑1 (MQ=255)

                                                                                                       aaCCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAt                             >  2:1681655/1‑140 (MQ=255)

                                                                                                        aCCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                            >  2:3626287/1‑140 (MQ=255)

                                                                                                        aCCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                            <  2:4768172/140‑1 (MQ=255)

                                                                                                        aCCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAAtt                            >  1:4820758/1‑140 (MQ=255)

                                                                                                         ccTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTg                           >  2:2071853/1‑140 (MQ=255)

                                                                                                         ccTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTg                           >  1:189639/1‑140 (MQ=255)

                                                                                                         ccTGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTg                           >  1:1813284/1‑140 (MQ=255)

                                                                                                           tGGTTTTGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTc                         <  2:2163930/140‑1 (MQ=255)

                                                                                                              ttttGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGTCATCGTGATCCATTACGACGGCGCGGGAGCTGGTGGTGCCCTGGGCGGGCGCAACTGTATATTTTTTTTCAGTCATGGGTAATTGTcctg                      >  2:1747054/1‑138 (MQ=255)

                                                                                                              ttttGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCg                      >  2:2753064/1‑140 (MQ=255)

                                                                                                               tttGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                     <  2:890224/140‑1 (MQ=255)

                                                                                                               tttGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                     <  2:2198703/140‑1 (MQ=255)

                                                                                                               tttGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                     <  1:3100823/140‑1 (MQ=255)

                                                                                                               tttGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATAATGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                     <  2:1430582/140‑1 (MQ=255)

                                                                                                               tttGGGTAGATTTGCTC‑AAATTCACGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTAGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCACTCATGTTTAATTGTCCCGt                     <  2:3817347/140‑1 (MQ=255)

                                                                                                               tttGGGTAGATGT‑CTCTAAATTCGCGCAGCGACACGCGCATGCAATTGCCCAAGTGATCCATTCCGACCGCGCGGCAGCTGGTGGTGCCCTGGTCGAGCGCAACGAGATATCTTTTTTCAGTCATGTTTAATTGCCCCGt                     <  2:3315772/140‑1 (MQ=255)

                                                                                                               tttGGGTACGGTTGCTC‑ACATTCGCGCTGCGACACGATAATGATATTGGCATCGTGATCCATTACGACCGCGTGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt                     <  2:4565375/140‑1 (MQ=255)

                                                                                                                 tGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                   <  2:2882257/140‑1 (MQ=255)

                                                                                                                 tGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                   <  2:246235/140‑1 (MQ=255)

                                                                                                                 tGGGTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg                   >  1:2612681/1‑140 (MQ=255)

                                                                                                                   ggTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTc                 >  1:3576330/1‑140 (MQ=255)

                                                                                                                   ggTAGATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTc                 >  1:406965/1‑140 (MQ=255)

                                                                                                                       gATTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTACCGTAGTGatat             >  1:4133684/1‑140 (MQ=255)

                                                                                                                        aTTTGCTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGGCGAGCGCAACGATAGATTTTTGTTCAGTCATGTTTAATTGTCCCGTAGTCATAtt            >  2:4278247/1‑140 (MQ=255)

                                                                                                                             cTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGc       >  1:4438664/1‑140 (MQ=255)

                                                                                                                             cTC‑AAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGc       >  1:2912447/1‑140 (MQ=255)

                                                                                                                                   aTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc  >  1:4252497/1‑140 (MQ=255)

                                                                                                                                   aTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc  >  2:1992927/1‑140 (MQ=255)

                                                                                                                                   aTTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc  >  2:3592665/1‑140 (MQ=255)

CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTC‑AAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGC  >  NZ_CP025268/4118909‑4119178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`