Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,270,732	T→C	*68* (TAA→TAG)	CXP41_RS22090 ←	protein YjbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,270,732	0	T	C	100.0%	167.7 / NA	52	*68* (TAA→TAG)	CXP41_RS22090	protein YjbS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/20);  total (32/20)

GTAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGAT  >  NZ_CP025268/4270596‑4270860                                                                                                                                         |                                                                                                                                 gtAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGttaactaa                                                                                                                               <  1:2094806/140‑1 (MQ=255)           ccTTATATTAATTACCAATAACAAATAAAAAGGTCCTGTTATGAGAATAATCACCTTTAACCTCATCGGCAAAACAGCAAAGCGTGCACATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAAt                                                                                                                     <  2:50635/140‑1 (MQ=255)               atatTGATTACCAATATCAAATAAAACGGTTCTGTAATGAGAATAATCAGCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAAAATACATAAAGTCTCACTTACCCCTGATTTTGTTAACTAAAACAATAAAtaggt                                                                                                                 >  2:3007178/1‑136 (MQ=255)                     aTTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGaaaaa                                                                                                           >  1:1911117/1‑140 (MQ=255)                     aTTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGaaaaa                                                                                                           >  1:1912216/1‑140 (MQ=255)                     aTTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGaaaaa                                                                                                           >  2:4819206/1‑140 (MQ=255)                     aTTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGaaaaa                                                                                                           >  2:4819580/1‑140 (MQ=255)                     aTTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGaaaaa                                                                                                           >  2:4819679/1‑140 (MQ=255)                      ttACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTATTAACTAAATCAATAAATGCGTGAAAAAc                                                                                                          >  2:3152227/1‑140 (MQ=255)                       tACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACt                                                                                                         <  1:743242/140‑1 (MQ=255)                       tACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACt                                                                                                         >  2:4631031/1‑140 (MQ=255)                            atatCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACt                                                                                                    <  1:1481975/140‑1 (MQ=255)                               tCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGc                                                                                                 >  2:4336422/1‑140 (MQ=255)                                   aTAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATa                                                                                             <  1:778383/140‑1 (MQ=255)                                    tAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAg                                                                                            >  1:1651089/1‑140 (MQ=255)                                    tAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAg                                                                                            >  1:1651664/1‑140 (MQ=255)                                            tCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGAt                                                                                    <  2:1651664/140‑1 (MQ=255)                                            tCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGAt                                                                                    <  2:1651089/140‑1 (MQ=255)                                                     gagaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTtcttct                                                                           >  1:175298/1‑140 (MQ=255)                                                      agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAGTCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTtctc                                                                          >  1:3654240/1‑140 (MQ=255)                                                      agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGAt                                                                                    >  1:3502213/1‑130 (MQ=255)                                                      agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGAt                                                                                    <  2:3502213/130‑1 (MQ=255)                                                      agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTtctc                                                                          >  1:3715966/1‑140 (MQ=255)                                                      agaATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAAGAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTtctc                                                                          >  2:3033126/1‑140 (MQ=255)                                                        aataatCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCa                                                                        >  1:4189397/1‑140 (MQ=255)                                                           aatCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGt                                                                     >  2:2527277/1‑140 (MQ=255)                                                             tCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCt                                                                   >  2:3875535/1‑140 (MQ=255)                                                                  tttAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTCGTTAACTAAATCAAGAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTaaaaa                                                              >  1:2333532/1‑140 (MQ=255)                                                                          cctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTg                                                      <  1:3118211/140‑1 (MQ=255)                                                                          cctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTg                                                      <  2:2084396/140‑1 (MQ=255)                                                                           gtcGGCAAAACAGCAAATCGTGGAAATATACATAAGGGCTTTCTTATCCCTGGTTTTGGGAACTAAATCAATAAATGCGTGAAAAACTTTACTTGGAAGAGAACTTGATACTTCTTCTCCAGGTCTAAAAGATCtgtttc                                                     >  2:1190004/2‑138 (MQ=255)                                                                           ctcGGCAAAACAGCAAAGCGTGCAAATATACATCAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAACCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGc                                                     >  2:769986/1‑140 (MQ=255)                                                                           ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTtctc                                                                          >  1:1578190/1‑119 (MQ=255)                                                                           ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTtctc                                                                          <  2:1578190/119‑1 (MQ=255)                                                                           ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAACTCTTTTg                                                      >  1:379496/1‑139 (MQ=255)                                                                           ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTg                                                      <  2:379496/139‑1 (MQ=255)                                                                           ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGc                                                     >  2:769741/1‑140 (MQ=255)                                                                           ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGc                                                     >  1:4343337/1‑140 (MQ=255)                                                                           ctcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGGTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGc                                                     >  1:2987372/1‑140 (MQ=255)                                                                            tcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCa                                                    <  2:1912216/140‑1 (MQ=255)                                                                            tcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCa                                                    <  2:1911117/140‑1 (MQ=255)                                                                            tcGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCa                                                    <  2:2348240/140‑1 (MQ=255)                                                                                    aCAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTaa                                            <  1:2910749/140‑1 (MQ=255)                                                                                      aGCAAAGCGAACAAATATACATAAAGTCTTTCTTACAACTGATTTTGTTAACTAAATACATAAATGCGTGCAAAACTTCACTTGCAAAAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGAAATTTTTAACt                                          <  2:1325406/140‑1 (MQ=255)                                                                                               tGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGttaactaa                                                                                                                               >  1:2003713/1‑46 (MQ=255)                                                                                               tGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGttaactaa                                                                                                                               <  2:2003713/46‑1 (MQ=255)                                                                                                         cATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAAGGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATaaaaa                       >  1:4375147/1‑140 (MQ=255)                                                                                                         cATAAAGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAAAGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATaaaaa                       >  1:4375476/1‑140 (MQ=255)                                                                                                              aGTCTTTCTTACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAAt                  >  1:916327/1‑140 (MQ=255)                                                                                                                 ctttcttACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCtt               <  1:37720/140‑1 (MQ=255)                                                                                                                    tcttACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTgg            >  2:1830138/1‑140 (MQ=255)                                                                                                                      tgaCCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTGCTCCAGGTCTAACAAATCTTTTGCAATTGTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCa          <  1:3875535/138‑1 (MQ=255)                                                                                                                       tACCCCTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCAt         <  2:2029307/140‑1 (MQ=255)                                                                                                                          cccTGATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAag      >  2:1257901/1‑140 (MQ=255)                                                                                                                              gATTTTGTTAACTAAATCAATAAATGCGTGAAAAACTTTACTTGCAAGAGAACTTGATACTTCTTCTCCAGCTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGat  <  2:3392753/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 GTAAGTTTAACCTTATATTGATTACCAATATCAAATAAAAAGGTCCTGTAATGAGAATAATCACCTTTAACCTCCTCGGCAAAACAGCAAAGCGTGCAAATATACATAAAGTCTTTCTTACCCCTGATTTTGTTAATTAAATCAATAAATGCGTGAAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGAT  >  NZ_CP025268/4270596‑4270860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑