Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,354,806	A→G	*99* (TAA→TAG)	ghoS →	type V toxin‑antitoxin system endoribonuclease antitoxin GhoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,354,806	0	A	G	100.0%	275.3 / NA	83	*99* (TAA→TAG)	ghoS	type V toxin‑antitoxin system endoribonuclease antitoxin GhoS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/58);  total (25/58)

CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACT  >  NZ_CP025268/4354670‑4354937                                                                                                                                         |                                                                                                                                    ccTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCa                                                                                                                                  <  2:1672027/140‑1 (MQ=255) ccTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCa                                                                                                                                  <  2:1672201/140‑1 (MQ=255)   ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagg                                                                                                                                <  2:997429/140‑1 (MQ=255)   ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagg                                                                                                                                <  1:2952382/140‑1 (MQ=255)    tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                               <  1:945549/140‑1 (MQ=255)    tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                               <  1:2062733/140‑1 (MQ=255)    tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                               <  1:2062392/140‑1 (MQ=255)    tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                               <  2:2554032/140‑1 (MQ=255)    tCGCAATAACGACATCGCTAGCCGCTAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                               <  2:4627031/140‑1 (MQ=255)              gACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaagaa                                                                                                                     <  1:3862712/140‑1 (MQ=255)              gACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaagaa                                                                                                                     <  1:2170041/140‑1 (MQ=255)               agaTCGTTAGCAGCTAGTGAAATCGAAGATTTAATCAGGCGCAAATGCTTAGTCTTACCGGATATTGATTTTGACCTCCACATGATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaagaag                                                                                                                    <  2:1992166/138‑1 (MQ=255)               aCATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaagaag                                                                                                                    <  2:1607109/140‑1 (MQ=255)               aCATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaagaag                                                                                                                    <  1:4257222/140‑1 (MQ=255)               aCATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTGCCGTCAGTTTTACAAGTAGCCAGGAAGGATaagaag                                                                                                                    <  1:1115596/140‑1 (MQ=255)               aCATCGTTAGCCGCAAGTGAAATCGAAGAATTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATaagaag                                                                                                                    <  2:3587406/140‑1 (MQ=255)                     ttAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATa                                                                                                              <  1:2509654/140‑1 (MQ=255)                     ttAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATa                                                                                                              <  1:2509819/140‑1 (MQ=255)                     ttAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATa                                                                                                              <  1:2509950/140‑1 (MQ=255)                          cGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                         <  2:530464/140‑1 (MQ=255)                            cAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                               >  2:1741640/1‑116 (MQ=255)                            cAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCagga                                                                                                                               <  1:1741640/116‑1 (MQ=255)                             aaGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATgg                                                                                                      <  1:1756415/140‑1 (MQ=255)                               gTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                    <  2:2777327/140‑1 (MQ=255)                               gTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                    <  2:4938108/140‑1 (MQ=255)                               gTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATCACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                    <  1:692676/140‑1 (MQ=255)                                  aaaTCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTa                                                                                                 <  2:2609413/140‑1 (MQ=255)                                                aTCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATAtt                                                                                   <  1:3256775/140‑1 (MQ=255)                                                              cTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                     <  2:1642209/140‑1 (MQ=255)                                                               ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                                    <  1:847812/140‑1 (MQ=255)                                                                tAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAtt                                                                   <  1:4672873/140‑1 (MQ=255)                                                                    cTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                               <  2:481088/140‑1 (MQ=255)                                                                    cTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                               <  1:465591/140‑1 (MQ=255)                                                                    cTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                               <  2:1513631/140‑1 (MQ=255)                                                                    cTTACCGGATATTGATTTTGACCCAAACATTATGACAGTTGATGACTATTTCCGTCAATTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                               <  1:4629111/140‑1 (MQ=255)                                                                      tACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGtgtg                                                             <  2:1342534/140‑1 (MQ=255)                                                                      tACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGtgtg                                                             <  1:4733288/140‑1 (MQ=255)                                                                       aCCGGATGTTGATTTTGACCTCAACATTATGACAGGTGACGACTATTTCGGTCAGCTTTAAAAATAGGTAGGGAGGATAAGAAGAAAATAAAAAATGACACTATTCTCTAAAATAGTAATGTTTTAAGTGATTGgtgtta                                                            >  2:4107170/1‑138 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTGGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                            >  1:4049705/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                            >  1:1991117/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                            >  2:2073329/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                            >  2:2073311/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                            >  1:3929874/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                            >  2:4697514/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                            >  2:4115957/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGa                                                            >  1:3930142/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGGGATTGGTGTGa                                                            >  1:3879466/1‑140 (MQ=255)                                                                       aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTGTTATGTGATTGGTGTGa                                                            >  2:3155435/1‑140 (MQ=255)                                                                        ccGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGaa                                                           >  2:2134891/1‑140 (MQ=255)                                                                        ccGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGaa                                                           <  1:2302682/140‑1 (MQ=255)                                                                           gATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACat                                                        <  1:1827119/140‑1 (MQ=255)                                                                                tGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCt                                                   <  2:3274165/140‑1 (MQ=255)                                                                                   ttttGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTg                                                <  1:595826/140‑1 (MQ=255)                                                                                           tCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTAAAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATc                                        <  2:1358748/140‑1 (MQ=255)                                                                                            cAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCt                                       >  1:1272131/1‑140 (MQ=255)                                                                                             aaCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTg                                      <  2:5063982/140‑1 (MQ=255)                                                                                              aCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTgg                                     <  1:4715972/140‑1 (MQ=255)                                                                                                    tGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTGTTTAGGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTAtc                               >  2:659140/1‑140 (MQ=255)                                                                                                    tGACAGTTGATGACTATTTCCGTCAGTCTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAGTATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTAACTGGTTTAta                               >  2:3750448/1‑139 (MQ=255)                                                                                                         gTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                     <  2:2565322/97‑1 (MQ=255)                                                                                                         gTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                     >  1:2565322/1‑97 (MQ=255)                                                                                                          ttGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACAt                         >  1:1327359/1‑140 (MQ=255)                                                                                                          ttGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACAt                         >  2:3398244/1‑140 (MQ=255)                                                                                                          ttGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATGTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACAt                         >  1:3240497/1‑140 (MQ=255)                                                                                                            gatgaCTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATga                       >  1:4472706/1‑140 (MQ=255)                                                                                                            gatgaCTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATga                       >  2:104168/1‑140 (MQ=255)                                                                                                                    tttCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgt                                                                <  2:4227249/91‑1 (MQ=255)                                                                                                                    tttCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAacac               >  2:4342305/1‑140 (MQ=255)                                                                                                                    tttCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTACGTGATTGgt                                                                >  1:4227249/1‑91 (MQ=255)                                                                                                                       ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  2:1153825/140‑1 (MQ=255)                                                                                                                       ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  1:982366/140‑1 (MQ=255)                                                                                                                       ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  1:104168/140‑1 (MQ=255)                                                                                                                       ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  1:2134891/140‑1 (MQ=255)                                                                                                                       ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  2:4635743/140‑1 (MQ=255)                                                                                                                       ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  1:407826/140‑1 (MQ=255)                                                                                                                       ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  1:108346/140‑1 (MQ=255)                                                                                                                       ccGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATGGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACata            <  1:108787/140‑1 (MQ=255)                                                                                                                            aGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGt       <  2:350505/140‑1 (MQ=255)                                                                                                                            aGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGt       <  1:3270657/140‑1 (MQ=255)                                                                                                                            aGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGt       <  2:1214242/140‑1 (MQ=255)                                                                                                                            aGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGt       >  1:1961836/1‑140 (MQ=255)                                                                                                                            aGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGt       <  1:3270763/140‑1 (MQ=255)                                                                                                                                 tACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACt  <  1:4672106/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTACT  >  NZ_CP025268/4354670‑4354937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑