BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,424,719	T→C	V203A (GTC→GCC)	ulaD →	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,424,719	0	T	C	100.0%	850.9 / NA	256	V203A (GTC→GCC)	ulaD	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (142/114); total (142/114)

Only 100 of 256 total aligned reads displayed.

TTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTG  >  NZ_CP025268/4424587‑4424852

tttCCGCTAGGGGCTTCGAGGTCCCCGTCGGCGGACGCCTGGCGCTGGAAGTTCTGCCGCGGTTCACGGGCATTCGGATTCACGTATTTAACGCGGGCCGTAGTTTCCGGGATGCCGCTTCTCCGGTGGAAGCCGCGCGt                                                                                                                                <  2:542041/140‑1 (MQ=255)

tttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGt                                                                                                                                <  1:4049430/140‑1 (MQ=255)

 ttCCGATATGGGCTTCAAAGTCACGGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCGTTATCGCGGGCCGGAGTATCCGTGATGCCGCTTCTCCGGGGGAAGCCGCACGTc                                                                                                                               >  1:1325581/1‑140 (MQ=255)

 ttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTc                                                                                                                               >  1:2136165/1‑140 (MQ=255)

      atatGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTc                                                                                                                          >  1:3354630/1‑140 (MQ=255)

       tatGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCa                                                                                                                         >  1:1142927/1‑140 (MQ=255)

         tGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCaaa                                                                                                                       >  2:2560298/1‑140 (MQ=255)

           ggCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACg                                                                                                                     >  1:3419925/1‑140 (MQ=255)

            gCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGt                                                                                                                    >  1:2618055/1‑140 (MQ=255)

            gCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGt                                                                                                                    >  1:4683553/1‑140 (MQ=255)

              ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                                  <  1:4901694/140‑1 (MQ=255)

              ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                                  <  1:3683110/140‑1 (MQ=255)

              ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                                  <  1:4872502/140‑1 (MQ=255)

              gtCAAAGTCGCCGTCACCGGAGGACTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTACTCCGTTTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                                  <  2:294949/139‑1 (MQ=255)

                cAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCa                                                                                                                <  1:2080997/140‑1 (MQ=255)

                cAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCa                                                                                                                >  2:4287561/1‑140 (MQ=255)

                   ggtcgccgtcggcGGAGGCCGGGCGCTGGAAGATCTGCCGCTTTTCAGGGGTGTTCCGATTCGCGTCTTTCTCGGGGGCCGTAGTACCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCg                                                                                                             <  2:3591165/139‑1 (MQ=255)

                   gcggaccgtgaccgGAGGCCGGCCGCTGCGAGATCAACCGCCGTTCAGGGGTATGCTGCATCGCCCCTGTCTCCCGGGCCGTGGCATCCGTGGTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCg                                                                                                             <  2:2846476/136‑1 (MQ=255)

                   aGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCg                                                                                                             <  1:2521488/140‑1 (MQ=255)

                      caccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTg                                                                                                          >  1:227803/1‑140 (MQ=255)

                       accgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGa                                                                                                         >  2:271685/1‑140 (MQ=255)

                        ccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAGACGTTTCATCGCTgca                                                                                                        >  2:1671673/1‑138 (MQ=255)

                           tcgccgGAGGGCTGGCGCTGGAAGATCGGCCGCTGTTCAGGGGTAGTCCGATTCACGTCTTTATCGCGGGCCGTAGTAGCCGTGAGGCCGCTTCTCCGGTGGAAGCCGCGCGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  2:884054/137‑1 (MQ=255)

                           tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGGGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  1:1227974/140‑1 (MQ=255)

                           tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  1:3887655/140‑1 (MQ=255)

                           tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  1:375655/140‑1 (MQ=255)

                           tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  1:703429/140‑1 (MQ=255)

                           tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  1:302354/140‑1 (MQ=255)

                           tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  1:4169208/140‑1 (MQ=255)

                           tcaccgGAGGACTGGCGGTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  1:3146922/140‑1 (MQ=255)

                           gcgccgGAGGCGTGGCGGTGGAAGACCTGCCGCTTAACAGGCGTATCCCGATTCGCTTCTTTATCGCGGGCCGTAGTATCCGTGAGGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                     <  2:1544552/137‑1 (MQ=255)

                              ccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGCTTCAGGTCATTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTgg                                                                                                  <  1:3650952/140‑1 (MQ=255)

                                  aGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCt                                                                                              <  1:1798653/140‑1 (MQ=255)

                                   ggCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTa                                                                                             <  1:1220644/140‑1 (MQ=255)

                                      cTGGGGCTGGGAGATCTGCCGCTCTTCCAGCGTCGTCCGGTTCTCCTGTTTGTCGCGTGCCGTGGGATCCGTGGTGCCGCTTCTCCGGGGGCAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                          <  2:3468301/140‑1 (MQ=255)

                                      cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                          <  1:2240009/140‑1 (MQ=255)

                                      cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                          <  1:4608227/140‑1 (MQ=255)

                                       tGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGa                                                                                         >  1:3507955/1‑140 (MQ=255)

                                        ggCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCGTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAg                                                                                        <  2:2148270/140‑1 (MQ=255)

                                         gcgcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGc                                                                                       >  1:4909729/1‑140 (MQ=255)

                                           gcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCgg                                                                                     <  1:1517879/140‑1 (MQ=255)

                                               gAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTACGGAGCGGatat                                                                                 >  2:2039608/1‑140 (MQ=255)

                                                    tCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGGAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtgttga                                                                            >  1:4515687/1‑139 (MQ=255)

                                                    tCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtgttgt                                                                            >  1:4985561/1‑140 (MQ=255)

                                                     cTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc                                                                           <  1:4378180/140‑1 (MQ=255)

                                                     atGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTG‑TGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTcc                                                                          <  1:4525955/139‑1 (MQ=255)

                                                       gCCGCTGTACAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCCTGCTGCCGCTTCTCCGTTGGAAGCCGCACGTCAGTCCAAACGTTCCATCTCTGTACTGTGGGGATACGGGGCGGAGATGTTGTCca                                                                         >  2:3053463/1‑140 (MQ=255)

                                                              ttCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAAt                                                                  <  1:140885/140‑1 (MQ=255)

                                                                        ttCCGATTTACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGAGGTGTTGTCCAAACAAATCCAGCtttgt                                                        >  2:1471117/1‑137 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        >  2:1023946/1‑140 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        <  1:1022539/140‑1 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        >  1:4880477/1‑140 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        >  2:3758943/1‑140 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        >  2:4205673/1‑140 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        <  1:4302811/140‑1 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCGAAGGAGCTGATATTTTGTCCAAACAAATCCCGCTAGGc                                                        >  1:4134245/1‑140 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCGAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        >  2:4853582/1‑140 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACCGTGGGGCTCAGGAGCGGTTATGTTGTCCAAACAAATCCCGCTTGGc                                                        >  2:1394401/1‑140 (MQ=255)

                                                                        ttCCGATTCACGTCTTTATCGCGGGCCGTACCTTCTGTAGTGCCGCTGCTCCTGTGTCATCCACACGTCTGACGCAGCGTTCGATCTCTCGCGTGTGGGGCTCGGGAGCGGCTATGTCATCAAAACAAATACCGCATGGc                                                        >  2:1218879/1‑140 (MQ=255)

                                                                          ccGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCAt                                                      <  1:2449347/140‑1 (MQ=255)

                                                                              ttCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTAt                                                  >  1:2406589/1‑140 (MQ=255)

                                                                                  cGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCATGGCATCTATGaaa                                              >  2:3723504/1‑140 (MQ=255)

                                                                                       ttATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAgcg                                         <  1:1165069/140‑1 (MQ=255)

                                                                                        tATCGCGGGCCGTAGTATCCGTGATGCCGCTGCTCCTGTGGAAGCCGCACGTCAGTTCAAAGGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGGCCACACAAATCCCGCTTCGCATCTATGGAAAAgggc                                        >  2:3971450/1‑140 (MQ=255)

                                                                                         aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTACGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                       <  2:2852365/140‑1 (MQ=255)

                                                                                         aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                       <  1:1473891/140‑1 (MQ=255)

                                                                                           cgcgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCGGCTTGGCATCTATGAAAAAGCGCTcc                                     >  1:5109357/1‑140 (MQ=255)

                                                                                             cgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        >  2:1309695/1‑119 (MQ=255)

                                                                                             cgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                        <  1:1309695/119‑1 (MQ=255)

                                                                                             cgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTcccc                                   >  1:285435/1‑140 (MQ=255)

                                                                                             cgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACGGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCGcccc                                   >  1:591492/1‑140 (MQ=255)

                                                                                              gggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccg                                  <  1:1639591/140‑1 (MQ=255)

                                                                                               ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgc                                 >  2:4300103/1‑140 (MQ=255)

                                                                                               ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgc                                 >  1:2017342/1‑140 (MQ=255)

                                                                                                   gtggtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCggg                             <  2:3531198/137‑1 (MQ=255)

                                                                                                   gtagtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCggg                             <  1:95350/140‑1 (MQ=255)

                                                                                                    tagtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCgggg                            >  1:3952313/1‑140 (MQ=255)

                                                                                                          ccGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTCGCATCTATGAAAAAGCGCCCCCCGCCGGGGAGTgct                      >  2:2078658/1‑140 (MQ=255)

                                                                                                           cGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctg                     >  2:1182262/1‑140 (MQ=255)

                                                                                                             gggtGCCGCGGCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggc                   <  2:475773/137‑1 (MQ=255)

                                                                                                              gATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGTTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGggctggct                  >  1:4669200/1‑140 (MQ=255)

                                                                                                              gATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct                  >  1:2565422/1‑140 (MQ=255)

                                                                                                              gATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct                  >  2:5066936/1‑140 (MQ=255)

                                                                                                              gATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct                  >  2:2895512/1‑140 (MQ=255)

                                                                                                              gATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGATGGGCTAAGGAGCGGATATGTTGTACAAACAAATCCCGTTTTGAATCTATGAAAAAGCACTCCCCGCCGGGGAGggctggct                  >  2:3566828/1‑140 (MQ=255)

                                                                                                               aTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCACACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggctg                 >  2:2088151/1‑140 (MQ=255)

                                                                                                               aTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggctg                 >  1:2371153/1‑140 (MQ=255)

                                                                                                               aTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggctg                 >  1:3581065/1‑140 (MQ=255)

                                                                                                               aTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATATATGAAAAAGCGCTCCCCGCCGGGGAGTgctggctg                 >  1:5037782/1‑140 (MQ=255)

                                                                                                                tGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctttttca                <  1:174600/140‑7 (MQ=255)

                                                                                                                tGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg                <  1:1573892/140‑1 (MQ=255)

                                                                                                                 gCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa               >  1:2718488/1‑140 (MQ=255)

                                                                                                                 gCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa               >  2:2333588/1‑140 (MQ=255)

                                                                                                                 gCCGCTTCTCCGGTGGAAGCCGCACATCAGTTCAGACGTTCCATCGCTGTACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCTCCGGGGAGTACTGGCTGGa               >  2:2988087/1‑140 (MQ=255)

                                                                                                                     cTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGAAGAACTGTGGGGCTAAGGAGCGGATATGTAGTCCAAACAAATCACGCTTGGCATCTATGAAAAAGCACTCTACGCCGGGGAGTGcgggctgggaagg           >  2:4095675/1‑135 (MQ=255)

                                                                                                                      gtctcCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAGCAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGcc          <  2:1951549/139‑1 (MQ=255)

                                                                                                                           gggTGGAAGCCGCACGTGAGTTCAACCGTTCCATCGCTGGACTGTGGGGCTAAGGAGCGGATATGTTGTCCACACAAATCCCGCTGGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTCGAACGCCTGCaa     <  2:3111755/139‑1 (MQ=255)

                                                                                                                           cGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCaa     >  1:2811884/1‑140 (MQ=255)

                                                                                                                            ggTGGAAGCCGCACGGCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAAc    >  1:106112/1‑140 (MQ=255)

                                                                                                                              gggAAGCCGCACGTCAGTTTAAACGTTCCATCGCAGTACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATACCGCTTGGCATCTATGAAAACGCGCTCCGCGCCGGGGAGTGCTGGCTGGAACGCCGGCAAccg  >  2:1247973/2‑138 (MQ=255)

TTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTG  >  NZ_CP025268/4424587‑4424852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`