Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,476,316 A→G pseudogene (259/585 nt) bdcR → TetR/AcrR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,476,3160AG99.7% 1124.0 / NA 331pseudogene (259/585 nt)bdcRTetR/AcrR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (149/181);  minor base T (0/1);  total (149/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 331 total aligned reads displayed.

TTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCA  >  NZ_CP025268/4476180‑4476452
                                                                                                                                        |                                                                                                                                        
 tATCTTGGTATTAGCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGGACGGGAGCTATTCCGCTTGCCGATATTTGTCGTGATGATCGTCCGGTAGGCGa                                                                                                                                      <  2:1957787/140‑1 (MQ=255)
 tATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGa                                                                                                                                      <  1:5084435/140‑1 (MQ=255)
 tATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGa                                                                                                                                      <  1:4558496/140‑1 (MQ=255)
 tATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGa                                                                                                                                      <  1:4511119/140‑1 (MQ=255)
  aTCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAg                                                                                                                                     >  1:297415/1‑140 (MQ=255)
     ttGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGc                                                                                                                                  >  2:1216512/1‑140 (MQ=255)
       ggTATTAACCCCCCGAGCCTCTACGCAGCTCTTGGCAGTAAAGCGGGGTGGTTTATCCGTGTACTCAATGAATACGGCGGAATGGAAGCTATTCCGCTTGCCGATATGCTGCGTGATGATCGTCCGCGAGGCGAGTGcag                                                                                                                                >  2:479807/1‑138 (MQ=255)
         tATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTgg                                                                                                                              >  1:2811389/1‑140 (MQ=255)
           ttAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGtt                                                                                                                            <  1:1281045/140‑1 (MQ=255)
           ttAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGtt                                                                                                                            >  2:1863233/1‑140 (MQ=255)
            tAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTg                                                                                                                           <  2:2068842/140‑1 (MQ=255)
            tAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTg                                                                                                                           <  1:2613653/140‑1 (MQ=255)
             aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGa                                                                                                                          <  1:384492/140‑1 (MQ=255)
             aaCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGa                                                                                                                          <  2:1722757/140‑1 (MQ=255)
              aCCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAg                                                                                                                         >  2:4448578/1‑140 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  2:2648732/1‑140 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  2:4044239/1‑140 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  1:495968/1‑140 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  1:3107813/1‑140 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  1:1493677/1‑140 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  1:1459240/1‑140 (MQ=255)
                   ccGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTAtt                                                                                                                    >  1:2189227/1‑140 (MQ=255)
                        ccTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAaag                                                                                                               >  2:2127952/1‑140 (MQ=255)
                          tctACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAaagaa                                                                                                             >  2:4650329/1‑140 (MQ=255)
                            tACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCGGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAgc                                                                                                           >  1:5050389/1‑140 (MQ=255)
                             aCGCGGCTTTTGGCAGTAAAGGTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcg                                                                                                          <  2:1798615/140‑1 (MQ=255)
                             aCGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcg                                                                                                          <  1:1107058/140‑1 (MQ=255)
                              cgcgGCTGTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAgcgg                                                                                                         <  1:3454999/140‑1 (MQ=255)
                                 ggCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgc                                                                                                      <  1:4177887/140‑1 (MQ=255)
                                 ggCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgc                                                                                                      <  2:3565102/140‑1 (MQ=255)
                                 ggCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgc                                                                                                      >  2:1035468/1‑140 (MQ=255)
                                 ggCTTTTGGCAGTAAAGCTGGGTTATGTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATGCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgc                                                                                                      >  2:219849/1‑140 (MQ=255)
                                  gCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                                     <  2:3280473/140‑1 (MQ=255)
                                  gCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcg                                                                                                     <  2:4907357/140‑1 (MQ=255)
                                   cTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcgc                                                                                                    >  2:1667518/1‑140 (MQ=255)
                                   cTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGgcgcgc                                                                                                    >  1:3152123/1‑140 (MQ=255)
                                     tttGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCCATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAg                                                                                                  >  1:4287886/1‑140 (MQ=255)
                                      ttGGCAGAAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGa                                                                                                 >  2:4965140/1‑140 (MQ=255)
                                        ggCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGata                                                                                               >  2:2542436/1‑140 (MQ=255)
                                             tAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGcc                                                                                          >  1:4771336/1‑140 (MQ=255)
                                                     ggTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAAcggc                                                                                  >  1:3207985/1‑140 (MQ=255)
                                                        tATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACggcggc                                                                               <  2:4726447/140‑1 (MQ=255)
                                                        tATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACggcggc                                                                               <  2:5071554/140‑1 (MQ=255)
                                                          tttAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTg                                                                             >  2:3456374/1‑140 (MQ=255)
                                                            tAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTGTTAAAAGAAGCGGCGCGGAGCTATAGCCAAAACGGCGGCTgcg                                                                           >  1:4658526/1‑140 (MQ=255)
                                                            tAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcg                                                                           <  2:2291078/140‑1 (MQ=255)
                                                            tAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcg                                                                           <  1:182950/140‑1 (MQ=255)
                                                            tAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcg                                                                           <  2:2266311/140‑1 (MQ=255)
                                                             aGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTGGCCGATATTCTTCGTGGTGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                          <  2:2991/140‑1 (MQ=255)
                                                             aGCCGTGTACTCAATGAATACGTCGGTACGGAAGATATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                          <  1:175971/140‑1 (MQ=255)
                                                               ccGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctg                                                                        >  1:2007864/1‑140 (MQ=255)
                                                                 gtgtACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCgctggc                                                                      <  2:521137/140‑1 (MQ=255)
                                                                    tACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGt                                                                   >  1:2919951/1‑140 (MQ=255)
                                                                    tACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGAGATAGCCAAAACGGCGGCTGCGCTGGCTGt                                                                   >  1:2106610/1‑140 (MQ=255)
                                                                     aCTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                                  <  2:918286/140‑1 (MQ=255)
                                                                     aCTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTa                                                                  <  1:3721445/140‑1 (MQ=255)
                                                                       tCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAACCGGCGGCTGCGCTGGCTGTATg                                                                <  1:817876/140‑1 (MQ=255)
                                                                       tCAATGAATACGTAGGTACGGAAGCTACTCCGCTCGCCGATATTCTTCGTGATGCTCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAAATATAGCCAAAACGGCGGCTGCGCTGGCTGTATg                                                                <  2:3035222/140‑1 (MQ=255)
                                                                        cAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATgg                                                               >  1:1699843/1‑140 (MQ=255)
                                                                         aaTGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGACGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGt                                                              >  1:4116891/1‑140 (MQ=255)
                                                                              aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCTGCTGCGCTGGCTGTATGGTTCTTg                                                         >  1:4804396/1‑140 (MQ=255)
                                                                              aTACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTg                                                         <  1:4044239/140‑1 (MQ=255)
                                                                                aCGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGaa                                                       >  1:588625/1‑140 (MQ=255)
                                                                                 cGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAg                                                      <  1:3644975/140‑1 (MQ=255)
                                                                                  gTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAgg                                                     <  1:3595928/140‑1 (MQ=255)
                                                                                   tCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGt                                                    <  2:3364862/140‑1 (MQ=255)
                                                                                     ggTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGGAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTAt                                                  >  1:1896764/1‑140 (MQ=255)
                                                                                     ggTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTAt                                                  >  1:3960625/1‑140 (MQ=255)
                                                                                       tACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTc                                                <  1:4085787/140‑1 (MQ=255)
                                                                                        aCGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCa                                               <  2:4287886/140‑1 (MQ=255)
                                                                                            aaGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGt                                           >  2:572311/1‑140 (MQ=255)
                                                                                              ggtATTCCGCTTGCAGATATTCTTCGAGATGATCGTCGGGTAGGCGAGTGACTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGTCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  2:4211690/138‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:355641/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:1594162/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  2:4341228/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:3397052/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:2662739/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:2541216/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:2297511/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:1636753/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  2:703290/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:3936914/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:4434053/140‑1 (MQ=255)
                                                                                              gCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCa                                         <  1:4402086/140‑1 (MQ=255)
                                                                                                 aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGa                                      >  2:4559366/1‑140 (MQ=255)
                                                                                                 aTTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGa                                      >  2:2739009/1‑140 (MQ=255)
                                                                                                       cTTGCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCcaca                                >  1:697060/1‑140 (MQ=255)
                                                                                                          gCCGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAgc                             >  2:1095692/1‑140 (MQ=255)
                                                                                                           ccGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAgcg                            <  2:1412143/140‑1 (MQ=255)
                                                                                                            cGATATTCTTCGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTgcgc                                                                          >  2:3859344/1‑93 (MQ=255)
                                                                                                                      cGTGATGATCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGc                 >  2:375221/1‑140 (MQ=255)
                                                                                                                        tgatgaTCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCg               <  1:3297491/140‑1 (MQ=255)
                                                                                                                         gatgctCGTCCGGTAGGCGAGTGCCTGGTTGAGGTCTTCAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTGTTGAAGGTATTCATGGTCATGATCCACAAGCGCGTGATCTTGCCgc              >  2:3989537/1‑139 (MQ=255)
                                                                                                                         gatgatCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGt              >  2:1578420/1‑140 (MQ=255)
                                                                                                                          atgatCGTCCGGTAGGCGAGTTCCTGGTTGAGGTATTTAAAGAAGCGGCGCGCACATATAGCCGAAACGGCGGCTGCGCTGGCTTTATGGTTCTTGAATGTATTCATAGTCATTATCCACAAGCTCGTGATATTGCCGtt             <  2:4263046/140‑1 (MQ=255)
                                                                                                                          atgatCGGCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAATGAAGCGGCGCGCATATATAGCCAAAGCGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGtt             <  2:2346917/140‑1 (MQ=255)
                                                                                                                           tgatCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTc            <  2:3152123/140‑1 (MQ=255)
                                                                                                                             atCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCaa          <  1:4902085/140‑1 (MQ=255)
                                                                                                                             atCGTCCGGTAGGCGAGTGCCTGGTGGAGGTATTAAAAGAAGCGGCGCGCAGACATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGTAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTGCaa          <  2:4521183/140‑1 (MQ=255)
                                                                                                                              tCGTCCGGTAGGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCTGCTGCGCTGGCGGTATGGTTCGTGAAGGTATTCATATTCATGATCCACAAGCGCGTGATATTTCCGGTCaag         >  2:3907597/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTATCTTGGTATTAACCCCCCGAGCCTCTACGCGGCTTTTGGCAGTAAAGCTGGGTTATTTAGCCGTGTACTCAATGAATACGTCGGTACGGAAGCTATTCCGCTTGCCGATATTCTTCGTGATGATCGTCCGGTAAGCGAGTGCCTGGTTGAGGTATTAAAAGAAGCGGCGCGCAGATATAGCCAAAACGGCGGCTGCGCTGGCTGTATGGTTCTTGAAGGTATTCATAGTCATGATCCACAAGCGCGTGATATTGCCGTTCAATATTATCA  >  NZ_CP025268/4476180‑4476452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑