BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,608,154	G→A	noncoding (48/87 nt)	CXP41_RS23870 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,608,154	0	G	A	98.7%	732.7 / ‑5.4	229	noncoding (48/87 nt)	CXP41_RS23870	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (142/83); total (145/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 229 total aligned reads displayed.

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608020‑4608290

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTTGTTGATATGGCATCGCGTAGGCCGAAGGGGGAGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                      >  2:4131428/1‑140 (MQ=18)

  aCGTCCGTAAGTACACTAAAACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGAACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                    <  2:2284088/140‑1 (MQ=37)

  aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                    <  1:2975716/140‑1 (MQ=255)

  aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                    <  2:35474/140‑1 (MQ=255)

  aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                    >  1:2104075/1‑140 (MQ=255)

     tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAAAACTAacac                                                                                                                                 >  1:22341/1‑140 (MQ=255)

     tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGATATCGCATCGTTTGGTGCGAGGGGGGGGTCTTGAACCCCTACGTCCGTAGGAACACTAacac                                                                                                                                 >  2:2824980/1‑140 (MQ=37)

         tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                                                                                             >  1:320052/1‑140 (MQ=255)

         tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCCCCTCGTTTGGGGCGAGGCGGGGGGCTTTCACCCCCACGTCAGTAGGAACACTAACACCtag                                                                                                                             >  2:2759550/1‑138 (MQ=25)

             gACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt                                                                                                                         >  1:3417911/1‑140 (MQ=255)

              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTAATCTTTCTTATTTTGATATCCCCCCGCTTGGTGGCAGGCGGGTGGCTTTAACCTCCACGTCCGTAATTACACTACCACATGAAGCTa                                                                                                                        >  2:1572652/1‑140 (MQ=18)

               cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                                                                                       >  1:1592790/1‑140 (MQ=255)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAATACCTGAAGCTAgc                                                                                                                      >  1:183408/1‑140 (MQ=255)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                                                                                                      >  1:4174891/1‑140 (MQ=255)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCGCGTTTGGTGCGAGGGGGGGGACTTGAAGCCCCACGTCCGTAAGAACACTAACACCGGACGCTAgc                                                                                                                      >  2:2638794/1‑140 (MQ=37)

                acTAACACCGGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAATCATCTTACTTATATTGATATCCTCCCGTGTGGGGCGAGTGAGGGGGCTTTAACCCCCCCGTCCCTAAGAACACATACACCGCAAGCTAgc                                                                                                                      >  2:348288/1‑140 (MQ=16)

                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGCTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcgc                                                                                                                    >  1:4292908/1‑140 (MQ=255)

                  tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCCTGCTTACTTTATTTGATATCGCCTCGCCGCGTGCGAGGCGGGGGACTTGAACCCCCACGTCCGTAAGAACACAAACACCTACAGCTAgctc                                                                                                                    >  2:1641741/1‑138 (MQ=25)

                      acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGGGCGAGGGGGGTGACTTGAACCCCCACGTCCGTAACAACACTAACACCTAAAGCTAGTGCGTCt                                                                                                                >  2:1220667/1‑140 (MQ=37)

                       ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTAATCTTACTTTTTTTGATATCGCCACGTGTGGTGCCAGGGAGGGGACTTGCACCCTCACGTCCGTATGAACACTAAGACCTGAGGCTAGCGCGTcga                                                                                                               >  2:2015361/1‑138 (MQ=25)

                        cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGATGGGGGGGACTTGAACCTCCACGTCAGTAATAACACTAACACCTGAAGCTAGCGCGTCTat                                                                                                              >  2:1754802/1‑139 (MQ=37)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                            >  1:3556258/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                            >  1:4354622/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                            >  2:575526/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                            >  1:3220836/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                      >  1:4748785/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                      >  2:3456540/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                      >  1:2444190/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTACCGCGTCTACCAATTCCg                                                                                                      >  1:1399942/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGCTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                      >  2:2135941/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGCTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                      >  2:4809830/1‑140 (MQ=255)

                                aGCGCGTCGACCAATTCCGCCACCTTCGCACAGTCATCGTACTATAACATCGATCTCGCCGTTTGGTTGGAGGGTGGTGACTTGAAGCTCCACGTCCGTACGAACACGAACAAGTGAAGTTACCGCGTCTACCAAtacag                                                                                                      >  2:695097/1‑136 (MQ=12)

                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                     <  1:2599646/140‑1 (MQ=255)

                                 gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                     <  1:2165506/140‑1 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                    >  1:4016420/1‑140 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                    >  1:33476/1‑140 (MQ=255)

                                  cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATACCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                                                    >  1:3055305/1‑140 (MQ=255)

                                     gTCTTCCAATTCCGGCACCTTCGATCAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                                 <  2:2166496/140‑1 (MQ=21)

                                      tCTACCAATTCCGCCACCTTCGCACAGCAATATTACTTTAATTGATATGCTCCCGCTTAGGGGCAGGCTGGTGGCTTGCACCTTTTCGTCCGTAGTAACACTAACACATAAGGGTAGCGCGTCTACCATTTCCGCCACCt                                                                                                >  2:303640/1‑140 (MQ=255)

                                      tCTAACAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                                                                <  2:4211461/140‑1 (MQ=255)

                                       cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                                                               >  2:4169855/1‑140 (MQ=255)

                                           cAATTCGGCCACCTAGACACAGTCATCTTACTTGTTGTGATCTCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATGCCGCCACCTTCGcc                                                                                           <  2:4660704/140‑1 (MQ=17)

                                           cAATTCCGGCAACTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  2:3171298/140‑1 (MQ=255)

                                           cAATTCCGCCATGTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  1:1987947/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  2:1319354/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  1:4442447/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  1:3902632/140‑1 (MQ=255)

                                           cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCGCCATGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  2:1056954/140‑1 (MQ=255)

                                              ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                                                                                        >  1:3377420/1‑140 (MQ=255)

                                               tCCGCCACCTTCGAACAGTGATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                                                       <  2:290178/140‑1 (MQ=255)

                                                  gCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATGCCGCCACCTTCGCCCAGTGCg                                                                                    <  2:4601654/140‑1 (MQ=255)

                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                  <  1:3034812/140‑1 (MQ=255)

                                                         tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                                                             >  1:4952202/1‑140 (MQ=255)

                                                          cGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAatat                                                                            <  2:3677207/140‑1 (MQ=255)

                                                              caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                        <  1:370170/140‑1 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTg                                                                     >  1:4572827/1‑140 (MQ=255)

                                                                      ttACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                                                                <  1:3623909/140‑1 (MQ=255)

                                                                         cTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCC‑CCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                                                            >  1:805162/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGATTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCCAGCAATATCAACGTGCTGTTTggcg                                                           >  2:3793840/1‑138 (MQ=18)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCGGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTg                                                           >  2:1309199/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                           >  1:252230/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                           >  2:3080177/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                           >  1:3989252/1‑140 (MQ=255)

                                                                           ttttttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg                                                           >  2:2125821/1‑140 (MQ=255)

                                                                               ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑g                                                      >  1:5097777/1‑140 (MQ=255)

                                                                               ttGATATCGCCTCGATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGGAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCTCAGTGTGAGCAATATCAACGTGGTTTTTGGttggag                                                       >  2:2243194/1‑135 (MQ=18)

                                                                                   tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑ggggg                                                  <  2:4901028/140‑1 (MQ=255)

                                                                                      cGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gggggggg                                               >  2:1393040/1‑140 (MQ=255)

                                                                                            gTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGa                                         <  1:1424810/140‑1 (MQ=255)

                                                                                             tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGaa                                        >  1:2732513/1‑140 (MQ=255)

                                                                                             tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTACGA‑GGGGGGGGACTTgga                                        >  2:1931098/1‑138 (MQ=255)

                                                                                              ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAGCTGAGGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGGACTTGaa                                        <  1:64760/140‑1 (MQ=21)

                                                                                                 gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAAcccc                                    <  2:2077510/140‑1 (MQ=255)

                                                                                                  tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAAccccc                                   >  1:1779674/1‑140 (MQ=255)

                                                                                                  tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTACCAGCTATATCAAAGAGGTGTTACGTCCGA‑GGGGGGGGCCTTGAAccccc                                   >  2:674319/1‑140 (MQ=17)

                                                                                                   gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           >  2:1074517/1‑84 (MQ=31)

                                                                                                   gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTGGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                  <  2:2466382/140‑1 (MQ=255)

                                                                                                   gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                           <  1:1074517/84‑1 (MQ=14)

                                                                                                   gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                  <  1:5064342/140‑1 (MQ=255)

                                                                                                   gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCa                                  <  1:3307408/140‑1 (MQ=255)

                                                                                                    agAGGGGGGGGACTTGAACCACCACGTCCGTAAGAACCCTCACACCCGAAGATAGCGCGTCTACCACTGCCGCCACCTTGGCCCAGTGCGTGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCAc                                 <  2:4572827/139‑1 (MQ=25)

                                                                                                     ggggggggggACTTGAACCCCCACGTCCGTAAGAACACAAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACg                                <  2:692048/138‑1 (MQ=255)

                                                                                                     agggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGCGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACg                                >  1:2789659/3‑140 (MQ=255)

                                                                                                       ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGAGCGA‑GGGGGGGGACTTGAACCCCCACGTc                              >  2:5065714/1‑140 (MQ=255)

                                                                                                            gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTaa                         >  1:3684255/1‑140 (MQ=255)

                                                                                                            gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAGCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAGCCCCCACGTCCGTat                         >  1:2343838/1‑139 (MQ=255)

                                                                                                             ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  1:4694477/140‑1 (MQ=255)

                                                                                                                  tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGACTACCAATTCCGCCACCTTCGCCTAGTGCAATCACTAAAAACGTGTTTTTTCGTCCGA‑GGGGGGGGACTTTAACCGCCACGTACATACGTacac                   >  2:3418176/1‑140 (MQ=17)

                                                                                                                   gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  >  1:1938380/1‑140 (MQ=255)

                                                                                                                   gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  >  1:274372/1‑140 (MQ=255)

                                                                                                                   gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  >  1:2901062/1‑140 (MQ=255)

                                                                                                                    aaCCCACACGTCCGTCAGAACACTAACTCCTGAAGCTAGCGCGTATACCTAAACCACAAACCTAGCCCAGTGCGAGCAATATCAACGTGTTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                 <  1:82135/140‑1 (MQ=21)

                                                                                                                     aCCCCCACGTCCGTAAGAACACTAACCCCTGAAGCTAGCGCGTCTTCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                <  1:1543835/140‑1 (MQ=255)

                                                                                                                     aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                >  2:1663670/1‑140 (MQ=255)

                                                                                                                         ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc            >  1:1110796/1‑140 (MQ=255)

                                                                                                                         ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTATGGACACTAACAcc            >  1:1315658/1‑140 (MQ=255)

                                                                                                                          cACGTCCGTAAGAACACTAACACGTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTACGAACAATAGCAACGGGGGTTTTGGTCCGA‑GGGGGGGTGCTTGAACCCGCACCTCCTTAAGGACACTAACACCt           >  2:124102/1‑140 (MQ=21)

                                                                                                                          cACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGCACCCCCACGTCCGTAAGGACACTAACACCt           >  1:4814502/1‑140 (MQ=255)

                                                                                                                           aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTGTTTGCTGCGA‑GGGGGGGGACGTGAACCCCCACGTCCGTGAGGACAGTAACACCTg          >  2:3442351/1‑140 (MQ=25)

                                                                                                                                   aaGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTCGCTCCGA‑GGGGGGGGCCTTCAACCCCCACCTCCGCGAGGACACTAACACCTGAAGCTacc  >  2:3587459/1‑138 (MQ=25)

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608020‑4608290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`