Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 469,693 A→G *591* (TAA→TAG) 
N3S (AAT→AGT) 		 mdlA →
mdlB →		 SmdA family multidrug ABC transporter permease/ATP‑binding protein
SmdB family multidrug efflux ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268469,6930AG99.7% 1006.2 / ‑5.1 301*591* (TAA→TAG) 
N3S (AAT→AGT) 		mdlA
mdlB		SmdA family multidrug ABC transporter permease/ATP‑binding protein
SmdB family multidrug efflux ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (163/135);  total (164/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 301 total aligned reads displayed.

GGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAGCTA  >  NZ_CP025268/469556‑469828
                                                                                                                                         |                                                                                                                                       
  aCATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGtttt                                                                                                                                     >  1:1728726/1‑140 (MQ=255)
  aCATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGtttt                                                                                                                                     >  2:2089202/1‑140 (MQ=255)
      ttCGCCCAGCGTGGCAAGCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGCATCGCGATCAACAACTGGAGGCGGCGCGCGACGACGCTGCGGAAAATCGCAAGGAGGCCGTCGATGCGTAGATTTAGcc                                                                                                                                 >  2:4232018/2‑140 (MQ=255)
      atCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGcc                                                                                                                                 >  1:75319/1‑140 (MQ=255)
      atCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGcc                                                                                                                                 <  1:4727503/140‑1 (MQ=255)
       tCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCa                                                                                                                                >  1:1371516/1‑140 (MQ=255)
       tCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCa                                                                                                                                >  2:2225082/1‑140 (MQ=255)
        cGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCaa                                                                                                                               <  1:3440853/140‑1 (MQ=255)
          cccAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACt                                                                                                                             >  1:656790/1‑140 (MQ=255)
          cccAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACt                                                                                                                             >  1:3093742/1‑140 (MQ=255)
            cAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACtgt                                                                                                                           >  2:1796717/1‑140 (MQ=255)
            cAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACtgt                                                                                                                           >  1:200411/1‑140 (MQ=255)
            cAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACtgt                                                                                                                           >  2:802401/1‑140 (MQ=255)
            cAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGACGCCGTCGATGCGTAGTTTTAGCCAACtgt                                                                                                                           >  2:3586731/1‑140 (MQ=255)
            cAGCGGGGCAAGCATGATGTGCGGGCACAACAAAGCGGCTGGTATGGGGGTATGTATCGCTATCAAAAACTGGAGGGGGCGGTTGACGACGCAACGGAAAATGGCGAGGAGGCCGTCGAAGCGGAGTTGTAGCGAACtgg                                                                                                                           >  2:4311899/1‑139 (MQ=255)
              gCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTgg                                                                                                                         >  1:4298996/1‑140 (MQ=255)
              gCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTgg                                                                                                                         >  2:905677/1‑140 (MQ=255)
               cGTGGCACTCATGATGTGTTGGACCAACACAGCGGCTGGTATCGCGATATGTATCGCTATCAAGACCTGGCGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGc                                                                                                                        <  2:954059/140‑1 (MQ=255)
               cGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGc                                                                                                                        <  2:1123019/140‑1 (MQ=255)
               cGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGc                                                                                                                        >  2:471132/1‑140 (MQ=255)
               cGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGc                                                                                                                        >  2:448259/1‑140 (MQ=255)
               cGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGc                                                                                                                        <  2:426371/140‑1 (MQ=255)
                gTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGcc                                                                                                                       <  1:2764568/140‑1 (MQ=255)
                   gCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGAc                                                                                                                    <  1:1575201/140‑1 (MQ=255)
                    cAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACGACGGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGGTGCGTAGTTTTAGCCAACTGTGGCCGAct                                                                                                                   >  2:2996582/1‑140 (MQ=255)
                         atgatgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGATGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCACaa                                                                                                              >  2:3026067/1‑140 (MQ=255)
                         atgatgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCaa                                                                                                              >  2:3222405/1‑140 (MQ=255)
                         atgatgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCaa                                                                                                              >  2:1395421/1‑140 (MQ=255)
                         atgatgTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCaa                                                                                                              <  2:2640443/140‑1 (MQ=255)
                              gtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGcc                                                                                                         >  1:1263348/1‑140 (MQ=255)
                              gtgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGcc                                                                                                         <  1:3989266/140‑1 (MQ=255)
                              gtgCTGGCACAACAAACCGGCTTGTATCGCCATATGTATCGCTATCAACAACTGACGGCGGCGCCCGACGACGCTCCAGAAAAGCGCGAGGACGGCGTCCCTGCGTAGGTTTAGCCAACTGTCGCCGACTCTCAAGCGcc                                                                                                         >  2:2478015/1‑140 (MQ=255)
                              gtgCTGGAACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCAGGAAAATCGCGAGGAGGCAGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGcc                                                                                                         <  2:1102801/140‑1 (MQ=255)
                               tgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTCGTTTTAGCCAACGGTGGCCGACTCGCAAGCGCCt                                                                                                        >  2:1222241/1‑140 (MQ=255)
                               tgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCt                                                                                                        >  2:2688233/1‑140 (MQ=255)
                               tgCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCt                                                                                                        >  2:3720134/1‑140 (MQ=255)
                                gCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTg                                                                                                       <  1:3269697/140‑1 (MQ=255)
                                gCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTg                                                                                                       <  1:4559192/140‑1 (MQ=255)
                                 cTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGt                                                                                                      >  2:6034/1‑140 (MQ=255)
                                 cTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGt                                                                                                      <  1:4569535/140‑1 (MQ=255)
                                 cTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGt                                                                                                      >  2:3959792/1‑140 (MQ=255)
                                    gCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAg                                                                                                   >  1:3695559/1‑140 (MQ=255)
                                    gCACAACAAAGCGGCTGGGATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGcccgggag                                                                                                   >  1:2906978/1‑134 (MQ=255)
                                        aacaaAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTa                                                                                               <  2:1032478/140‑1 (MQ=255)
                                         acaaAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTAc                                                                                              >  1:249906/1‑140 (MQ=255)
                                         acaaAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTAc                                                                                              >  1:2737316/1‑140 (MQ=255)
                                         acaaAGCGGCTGGTATCGCGATATGTAGCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCGCCGGGAAATCGCGAGGAGGCCGTCGATGCGT‑AGTTTAGCGAACTGTGGACGACTCGCAAGCGCCGGTTACCGTACg                                                                                             >  2:1937434/1‑140 (MQ=255)
                                                 gCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGcc                                                                                      <  2:2431427/140‑1 (MQ=255)
                                                  cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCg                                                                                     >  1:989773/1‑140 (MQ=255)
                                                  cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCg                                                                                     <  1:11680/140‑1 (MQ=255)
                                                  cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCg                                                                                     <  1:351498/140‑1 (MQ=255)
                                                  cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCg                                                                                     >  1:2200202/1‑140 (MQ=255)
                                                  cTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCGCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCg                                                                                     >  1:4944433/1‑140 (MQ=255)
                                                      tATCGCGATCTGAATCGCTATCATCAGCTGGAGGCGGCGCTCGAGTACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGGCGACTCTCAAGCGCCGGTTAGCGTACGGTTCGCCGTGGc                                                                                 <  2:2906877/140‑1 (MQ=255)
                                                           cgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaaa                                                                            >  2:1066394/1‑140 (MQ=255)
                                                           cgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaaa                                                                            <  1:1951993/140‑1 (MQ=255)
                                                           cgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaaa                                                                            >  2:1593419/1‑140 (MQ=255)
                                                           cgATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTaaa                                                                            <  1:3134928/140‑1 (MQ=255)
                                                                tGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCt                                                                       <  2:4970222/140‑1 (MQ=255)
                                                                     cGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGa                                                                  >  1:3251149/1‑140 (MQ=255)
                                                                      gCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGAt                                                                 >  2:3460580/1‑140 (MQ=255)
                                                                       cTATCAACAACTGGGGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGACGATGCGTAGTTTTAGCCAACTGTGGCCGACTCGCAAGCGCTTGTTAGCGTACGGTTCCCCGTGGCGAAAACAGCTGGCGAtt                                                                >  2:2275535/1‑140 (MQ=255)
                                                                        taccaacacCTCGACGCGGCGCTATACGACGCACCTGAAAATCGCGAGGATTTCTTCGATGCGTATTTTTAGCCAACTGTGGCCGTCTCTCAAGCGCCTGTTAGCGTACGTTTCGCCTTTGCGTAACCCGCTTGGGATTg                                                               <  2:1553987/137‑1 (MQ=255)
                                                                        tATGAACAACTGGGGGGGGCGCTCGAAGACGCTCCGGAAAAGCGCGAGGAGGCCGTCGAGGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCGGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTg                                                               <  2:341619/140‑1 (MQ=255)
                                                                        tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAGACCGCTGGGGATTg                                                               >  1:2616934/1‑140 (MQ=255)
                                                                        tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTg                                                               >  2:4109368/1‑140 (MQ=255)
                                                                        tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTg                                                               <  1:1010480/140‑1 (MQ=255)
                                                                        tATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTg                                                               <  1:5118276/140‑1 (MQ=255)
                                                                          tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGCGGCCGGCGATGCGTAGTTGTCGCCAACTGTGGCCGACTCTCAAGCGCCTGGTAGCGTACGGTTCGCCGAGGCGTAAACCGCTGGGGGTTGCg                                                             >  2:727925/1‑140 (MQ=255)
                                                                          tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCTGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCg                                                             >  1:4247334/1‑140 (MQ=255)
                                                                          tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCg                                                             >  1:1833067/1‑140 (MQ=255)
                                                                          tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCg                                                             >  1:1902820/1‑140 (MQ=255)
                                                                          tCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGCGCGCCGTGGCGTAAACCGCTGGGGATTGCg                                                             >  1:2227703/1‑140 (MQ=255)
                                                                           caacaaCTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCgg                                                            <  2:4953898/140‑1 (MQ=255)
                                                                             acaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTc                                                          <  1:448259/140‑1 (MQ=255)
                                                                             acaacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTc                                                          <  1:1876748/140‑1 (MQ=255)
                                                                               aacTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCt                                                        <  1:2043951/140‑1 (MQ=255)
                                                                                 cTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGGCGATGCGTAGTTTTCGCCAACAGTGGCCGAGTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGGCCtga                                                      >  2:4507947/1‑140 (MQ=255)
                                                                                    gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCtgatga                                                   >  1:1700527/1‑140 (MQ=255)
                                                                                    gAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCAGGGGATTGCGGTCCtgatga                                                   >  2:4827374/1‑140 (MQ=255)
                                                                                      ggcggcGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGatgatg                                                 >  1:2145879/1‑140 (MQ=255)
                                                                                           cgcTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGAGTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGCTTGCGGTCCTGATGATGTGGGt                                            >  2:4543784/1‑140 (MQ=255)
                                                                                           cgcTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGt                                            >  1:4153458/1‑140 (MQ=255)
                                                                                                   gacgCACCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTgcggcgg                                    <  2:3904069/140‑1 (MQ=255)
                                                                                                       cTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCggcggcggc                                >  1:1496426/1‑140 (MQ=255)
                                                                                                        tCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCa                               >  1:3708945/1‑140 (MQ=255)
                                                                                                           ggAAAATCGCGAGCAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGaa                            <  2:3035470/140‑1 (MQ=255)
                                                                                                                    cgAGGAGGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTggg                   >  1:5001088/1‑140 (MQ=255)
                                                                                                                     gaggagGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGc                  <  1:835903/140‑1 (MQ=255)
                                                                                                                     gaggagGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGc                  <  1:3314854/140‑1 (MQ=255)
                                                                                                                     gaggagGCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGc                  <  1:441300/140‑1 (MQ=255)
                                                                                                                        gaggCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCgc               <  2:1308965/140‑1 (MQ=255)
                                                                                                                        gaggCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCgc               <  2:656790/140‑1 (MQ=255)
                                                                                                                        gaggCCGTCGATGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCgc               <  1:2374211/140‑1 (MQ=255)
                                                                                                                                  aTGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAg     <  2:843398/140‑1 (MQ=255)
                                                                                                                                  aTGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAg     <  1:2819264/140‑1 (MQ=255)
                                                                                                                                  aTGCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAg     <  1:3551107/140‑1 (MQ=255)
                                                                                                                                    gCGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAGCt   <  2:1700461/140‑1 (MQ=255)
                                                                                                                                    gCGTACTTTTAGCGAACTGTGGCCGACTCTCAGGCGCCTGTTAGCGTACGGTTCGCCGTTGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAGCt   <  2:465497/140‑1 (MQ=255)
                                                                                                                                     cGTAGTTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAGCTa  >  1:1793478/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GGACATATCGCCCAGCGTGGCAATCATGATGTGCTGGCACAACAAAGCGGCTGGTATCGCGATATGTATCGCTATCAACAACTGGAGGCGGCGCTCGACGACGCTCCGGAAAATCGCGAGGAGGCCGTCGATGCGTAATTTTAGCCAACTGTGGCCGACTCTCAAGCGCCTGTTAGCGTACGGTTCGCCGTGGCGTAAACCGCTGGGGATTGCGGTCCTGATGATGTGGGTTGCGGCGGCGGCAGAAGTCAGTGGGCCGCTGCTTATCAGCTA  >  NZ_CP025268/469556‑469828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑