BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	476,075	A→G	118 (TAA→TAG)	ybaA →	DUF1428 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	476,075	0	A	G	100.0%	1047.7 / NA	311	118 (TAA→TAG)	ybaA	DUF1428 domain‑containing protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (133/178); total (133/178)

Only 100 of 311 total aligned reads displayed.

ACCCTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTA  >  NZ_CP025268/475939‑476211

aCCCTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGGCCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTa                                                                                                                                       <  1:261805/140‑1 (MQ=255)

aCCCTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTa                                                                                                                                       <  2:1034277/140‑1 (MQ=255)

aCCCTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGGTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTa                                                                                                                                       <  2:3827254/140‑1 (MQ=255)

  ccTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGc                                                                                                                                     >  1:2515366/1‑140 (MQ=255)

  ccTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGc                                                                                                                                     >  1:2485274/1‑140 (MQ=255)

   cTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCTAGTCAATCATCGACGAATAGGTAGCg                                                                                                                                    >  1:1945371/1‑140 (MQ=255)

    ttCAAAAGAGGTCCGCAACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGCCGGCAAGCGAAGGATCTATAGCGGATGCGATTCAATCATCGACGAATAGGTAGCgg                                                                                                                                   >  2:3737162/1‑139 (MQ=255)

     tCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTg                                                                                                                                  <  2:1361466/140‑1 (MQ=255)

       aaaaGTGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGAc                                                                                                                                <  1:3920751/140‑1 (MQ=255)

       aaaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGAc                                                                                                                                >  2:2395700/1‑140 (MQ=255)

       aaaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGAc                                                                                                                                <  2:749590/140‑1 (MQ=255)

       aaaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGAc                                                                                                                                >  1:4858383/1‑140 (MQ=255)

       aaaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGAc                                                                                                                                >  2:4307335/1‑140 (MQ=255)

       aaaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGGCGGACCCACGGATGAAAGAGTGCGGCGAGTCAATGCCGTTTGACGGCAAGCGAAGGATCTATGGCGGATTCGAGTCAATCGTCGACGAATAGGTAGCGTGAc                                                                                                                                <  2:2201556/140‑1 (MQ=255)

          agagGTCCGCGACGCTTCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACggg                                                                                                                             <  1:2880563/140‑1 (MQ=255)

              gTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCtgt                                                                                                                         >  1:2227052/1‑140 (MQ=255)

              gTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCtgt                                                                                                                         >  2:3148145/1‑140 (MQ=255)

                ccGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTgc                                                                                                                       <  2:2588586/140‑1 (MQ=255)

                ccGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTgc                                                                                                                       <  2:2693199/140‑1 (MQ=255)

                                cAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGccc                                                                                                       >  2:4540525/1‑140 (MQ=255)

                                cAAAAGATGAGGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATTATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGccc                                                                                                       >  2:116860/1‑140 (MQ=255)

                                   aaGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTgc                                                                                                                       >  1:5122785/1‑121 (MQ=255)

                                   aaGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaa                                                                                                    >  1:3226181/1‑140 (MQ=255)

                                     gatgatGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaaa                                                                                                  >  1:2425944/1‑140 (MQ=255)

                                     gatgatGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaaa                                                                                                  <  2:292559/140‑1 (MQ=255)

                                        gatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTc                                                                                               >  2:4855750/1‑140 (MQ=255)

                                          tgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTcac                                                                                             <  1:860897/140‑1 (MQ=255)

                                               gACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATg                                                                                        <  2:3395801/140‑1 (MQ=255)

                                               gACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATg                                                                                        >  2:2505113/1‑140 (MQ=255)

                                                   cACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGc                                                                                    >  2:3914841/1‑140 (MQ=255)

                                                    aCGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                                   >  1:873815/1‑140 (MQ=255)

                                                    aCGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                                   <  1:3170646/140‑1 (MQ=255)

                                                    aCGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                                   <  2:1243481/140‑1 (MQ=255)

                                                         tGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                              <  2:4183785/140‑1 (MQ=255)

                                                               agTTCGGCGAGTCTATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGt                                                                        <  2:4443246/140‑1 (MQ=255)

                                                                  tCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATg                                                                                        >  1:1323492/1‑121 (MQ=255)

                                                                  tCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAg                                                                     >  1:1028376/1‑140 (MQ=255)

                                                                  tCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAg                                                                     >  1:387348/1‑140 (MQ=255)

                                                                  tCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAg                                                                     >  2:5121509/1‑140 (MQ=255)

                                                                      cGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAAt                                                                 <  1:173540/140‑1 (MQ=255)

                                                                           ccATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAAtcttct                                                            <  2:1071131/140‑1 (MQ=255)

                                                                             aTGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCttt                                                          <  2:3961524/140‑1 (MQ=255)

                                                                                ccGTTTGACGGCAAGCGACTGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCgg                                                       <  2:4391750/140‑1 (MQ=255)

                                                                                ccGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCgg                                                       <  2:4647709/140‑1 (MQ=255)

                                                                                ccGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCgg                                                       <  1:1288229/140‑1 (MQ=255)

                                                                                  gTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTa                                                     <  2:3279642/140‑1 (MQ=255)

                                                                                    ttGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGAAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAAt                                                   >  1:4985939/1‑140 (MQ=255)

                                                                                     tGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATg                                                  <  1:2702025/140‑1 (MQ=255)

                                                                                       aCGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGcc                                                <  1:2360489/140‑1 (MQ=255)

                                                                                       aCGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGcc                                                <  1:3752408/140‑1 (MQ=255)

                                                                                        cGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCTTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCt                                               <  1:2429121/140‑1 (MQ=255)

                                                                                        cGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCt                                               <  1:1808133/140‑1 (MQ=255)

                                                                                        cGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCt                                               <  1:1871684/140‑1 (MQ=255)

                                                                                        cGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCt                                               <  2:3009838/140‑1 (MQ=255)

                                                                                        cGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCt                                               <  1:3993720/140‑1 (MQ=255)

                                                                                        cGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTCTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCt                                               <  2:1870979/140‑1 (MQ=255)

                                                                                         ggCAAGCGAATGATCTATGGCGGATTCGAGTTAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTAAGCCCAAAAATTCACAAGTGTTGCTAGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCtt                                              <  2:2126787/140‑1 (MQ=255)

                                                                                           cAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAACGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGc                                            >  2:2785097/1‑140 (MQ=255)

                                                                                             aGCGAATGATCTATGGCGGATTCGAGTCAATCATCGATGAATAGGTAGCGTTACGGGCTGAGCGGCGTGCGGTTTGCCATATAATTGACGAATGTTGCTCGGCCCAGCTTTAGAAATCTTCTTTCGGTAATGCCttgttg                                          >  2:1601504/1‑140 (MQ=255)

                                                                                             aGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTg                                          >  2:1431885/1‑140 (MQ=255)

                                                                                             aGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTg                                          >  2:4958835/1‑140 (MQ=255)

                                                                                              gCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGt                                         <  1:3530958/140‑1 (MQ=255)

                                                                                              gCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGt                                         <  1:752892/140‑1 (MQ=255)

                                                                                              gCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGt                                         <  1:2179066/140‑1 (MQ=255)

                                                                                                   tGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagc                                    <  2:1544104/140‑1 (MQ=255)

                                                                                                   tGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagc                                    <  2:3095731/140‑1 (MQ=255)

                                                                                                   tGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagc                                    <  1:2783822/140‑1 (MQ=255)

                                                                                                   tGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGagc                                    <  1:3295501/140‑1 (MQ=255)

                                                                                                          tGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGGGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGGAATGCCTCGCTGTAGAGCCAGCCCt                             <  2:3626830/140‑1 (MQ=255)

                                                                                                            gCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGa                           <  2:1694034/140‑1 (MQ=255)

                                                                                                            gCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGa                           <  2:3719581/140‑1 (MQ=255)

                                                                                                               gATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                        <  2:2308552/140‑1 (MQ=255)

                                                                                                               gATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                        <  1:988268/140‑1 (MQ=255)

                                                                                                               gATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                        >  1:3409242/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATTGACGAATAGGTAGCGTGACGGGCTATGCGGCGTGGCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCGGCGTGAGAAAGCGTATTTCGGGAATGCCTTGCTGTAGAGCGAGCCCGGaggat                       >  2:1498350/1‑136 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGcc                                                >  2:699318/1‑115 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGcc                                                <  1:699318/115‑1 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  2:579673/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  1:1166413/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  2:3468285/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  1:1655864/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  1:2314959/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  1:4712641/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  1:4933867/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  1:928090/1‑140 (MQ=255)

                                                                                                                aTTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGt                       >  2:3002795/1‑140 (MQ=255)

                                                                                                                 ttCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCGGACCGTa                      >  2:803677/1‑140 (MQ=255)

                                                                                                                    gAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCATCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTg                   >  1:3647925/1‑140 (MQ=255)

                                                                                                                    gAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTg                   <  1:590415/140‑1 (MQ=255)

                                                                                                                    gAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTg                   >  1:4318097/1‑140 (MQ=255)

                                                                                                                      gTCAATCACCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGTCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCa                 <  2:4814409/140‑1 (MQ=255)

                                                                                                                        cAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACg               <  2:4725661/140‑1 (MQ=255)

                                                                                                                        cAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACg               <  1:3341078/140‑1 (MQ=255)

                                                                                                                          atcatcGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGcc             <  1:1431885/140‑1 (MQ=255)

                                                                                                                             atcGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCACAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATg          <  1:4958835/140‑1 (MQ=255)

                                                                                                                             atcGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATg          <  1:5112241/140‑1 (MQ=255)

                                                                                                                             atcGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATg          <  1:4229769/140‑1 (MQ=255)

                                                                                                                                  cgaATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGc     <  2:3185824/140‑1 (MQ=255)

                                                                                                                                    aaTAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGt   <  1:4476224/140‑1 (MQ=255)

                                                                                                                                     aTAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATTTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTACTGCCTTCCTTTAGAGCCAGCCCTGACTGTAGTGCACGCAATGCTGGCGTa  >  1:4652699/1‑140 (MQ=255)

ACCCTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTA  >  NZ_CP025268/475939‑476211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`