BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,476,063	(A)_7→6	pseudogene (6/585 nt)	bdcR →	TetR/AcrR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,476,057	0	A	.	100.0%	1503.8 / NA	310	intergenic (‑80/‑1)	bdcA/bdcR	SDR family oxidoreductase/TetR/AcrR family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (198/112); total (198/112)

Only 100 of 320 total aligned reads displayed.

GGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATT  >  NZ_CP025268/4475918‑4476192

ggCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAATGACTCCTTGATTCATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTa                                                                                                                                         <  2:4925588/140‑1 (MQ=255)

   aCCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTaaaa                                                                                                                                      <  2:5252756/140‑1 (MQ=255)

          ccACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAAtc                                                                                                                              >  1:3514591/1‑140 (MQ=255)

            aCGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAAtctc                                                                                                                            <  1:5065996/140‑1 (MQ=255)

            aCGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAAtctc                                                                                                                            <  2:5442116/140‑1 (MQ=255)

            aCGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAAtctc                                                                                                                            >  1:4823776/1‑140 (MQ=255)

            aCGACTACCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAAtctc                                                                                                                            <  2:2778542/140‑1 (MQ=255)

             ggACTGCCAGCGAGGATGAGGACTGGCTTACCTGTAAAGGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTcg                                                                                                                           <  2:5368140/139‑1 (MQ=255)

             cGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTcg                                                                                                                           <  2:3972713/140‑1 (MQ=255)

             cGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTcg                                                                                                                           >  1:186301/1‑140 (MQ=255)

                 tGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGtt                                                                                                                       >  1:3769684/1‑140 (MQ=255)

                      ccaaGGAAGAGAACTGTCTGACCTGTAAACGAGGCCATAAGGACTCGTTGATTTATTAGGTAACATGCATTACAAAACAGTTCTAACTTTCTGTCAACAGGTTTTGTAATGGCCACT‑AAAAAACCATCTCGCGTTCCAgg                                                                                                                  <  2:3101343/137‑1 (MQ=255)

                        gAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGgtc                                                                                                                >  2:2908408/1‑140 (MQ=255)

                        gAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGgtc                                                                                                                >  2:753365/1‑140 (MQ=255)

                             tGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTccc                                                                                                           >  1:2304514/1‑140 (MQ=255)

                             tGAGAACTGTCTTACCTGTAAAAGCGACCATAAGGACTCCTTGATTTATTATGTGACATGCATTACGAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTccc                                                                                                           >  2:4636525/1‑140 (MQ=255)

                                gaACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGa                                                                                                        <  1:1309078/140‑1 (MQ=255)

                                    tGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGtt                                                                                                    >  1:4408842/1‑140 (MQ=255)

                                     gTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGttt                                                                                                   >  1:2524053/1‑140 (MQ=255)

                                      tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTc                                                                                                  >  1:5575877/1‑140 (MQ=255)

                                      tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTc                                                                                                  >  1:330448/1‑140 (MQ=255)

                                      tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTc                                                                                                  >  1:833718/1‑140 (MQ=255)

                                      tCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTc                                                                                                  >  1:1811404/1‑140 (MQ=255)

                                          aCCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc                                                                                              <  1:799347/140‑1 (MQ=255)

                                          aCCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTc                                                                                              <  2:3033755/140‑1 (MQ=255)

                                           ccTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTcc                                                                                             >  1:2489343/1‑140 (MQ=255)

                                           ccTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTcc                                                                                             >  2:4295297/1‑140 (MQ=255)

                                               tAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTAAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCTAGACGTTTCGCTCCTGAg                                                                                         >  2:3990840/1‑140 (MQ=255)

                                                aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGc                                                                                        >  2:109202/1‑140 (MQ=255)

                                                aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGc                                                                                        >  1:1143735/1‑140 (MQ=255)

                                                aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGc                                                                                        >  1:3331153/1‑140 (MQ=255)

                                                aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGc                                                                                        >  2:3433584/1‑140 (MQ=255)

                                                aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGc                                                                                        >  1:2635148/1‑140 (MQ=255)

                                                aaaaGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGc                                                                                        >  2:5100144/1‑140 (MQ=255)

                                                     cgcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCa                                                                                   >  2:3832936/1‑140 (MQ=255)

                                                     cgcCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCACGCGTTCCAGGTCGTCCAAGACGTTTCGCTCCTGAGCAGGCa                                                                                   >  2:2010132/1‑140 (MQ=255)

                                                                  tCCTTGATTTATTATGTAACATGCATTACAAGACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                      >  2:2975601/1‑140 (MQ=255)

                                                                  tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                      >  1:4649577/1‑140 (MQ=255)

                                                                  tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                      >  2:4493146/1‑140 (MQ=255)

                                                                  tCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCCGCGGCaa                                                                      >  2:4142793/1‑140 (MQ=255)

                                                                  tCCTTGATTTATTAGGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaa                                                                      >  1:694991/1‑140 (MQ=255)

                                                                   ccTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaaa                                                                     <  1:1163200/140‑1 (MQ=255)

                                                                   ccTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaaa                                                                     <  1:2967512/140‑1 (MQ=255)

                                                                    cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaaaa                                                                    >  2:2626083/1‑140 (MQ=255)

                                                                    cTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCaaaa                                                                    >  1:4311606/1‑140 (MQ=255)

                                                                      tGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGt                                                                  >  1:1466989/1‑140 (MQ=255)

                                                                            attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtttt                                                            >  1:1240640/1‑140 (MQ=255)

                                                                            attatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCtttt                                                            >  2:1219138/1‑140 (MQ=255)

                                                                             ttatGTAACGTGCATTACAAAACTGTTTTAACTTTCTGTCCACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCttttt                                                           <  2:895829/140‑1 (MQ=255)

                                                                              tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCCCT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTc                                                          >  2:5322382/1‑140 (MQ=255)

                                                                              tatGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTc                                                          >  2:1184756/1‑140 (MQ=255)

                                                                                tGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCAc                                                        >  2:4221797/1‑140 (MQ=255)

                                                                                   aaCATGCATTACAAGCCTGTTTTAAATTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGCCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCaa                                                     <  2:2723606/140‑1 (MQ=255)

                                                                                       tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAg                                                 >  1:3061477/1‑140 (MQ=255)

                                                                                       tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAg                                                 >  1:2409016/1‑140 (MQ=255)

                                                                                       tGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAg                                                 >  1:3680635/1‑140 (MQ=255)

                                                                                       gacATTACAAAAGTGTTTTAACTATCTTTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCGCGCGTTCCAGGGCGTTCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAg                                                 >  2:3346549/3‑140 (MQ=255)

                                                                                          aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCACAAACGtt                                              >  2:293924/1‑140 (MQ=255)

                                                                                          aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt                                              >  1:139672/1‑140 (MQ=255)

                                                                                          aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt                                              >  1:5544768/1‑140 (MQ=255)

                                                                                          aTTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGtt                                              >  2:4732036/1‑140 (MQ=255)

                                                                                           ttACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGttt                                             <  1:2112314/140‑1 (MQ=255)

                                                                                            tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTc                                            >  2:5478397/1‑140 (MQ=255)

                                                                                            tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTc                                            >  1:1576817/1‑140 (MQ=255)

                                                                                            tACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGATCACT‑AAAATACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTGTTTCACCAAAAAGGTTTc                                            >  1:3368669/1‑140 (MQ=255)

                                                                                               aaaaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGAt                                         <  2:4467207/140‑1 (MQ=255)

                                                                                                 aaCTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCCCT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAATCGGTTTCGATGc                                       >  1:2163272/1‑140 (MQ=255)

                                                                                                 aaCTGTTTTAACTGTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGc                                       >  2:588007/1‑140 (MQ=255)

                                                                                                    tGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGt                                    <  1:4698676/140‑1 (MQ=255)

                                                                                                      ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCa                                  >  1:408583/1‑140 (MQ=255)

                                                                                                      ttttAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCa                                  >  1:4585574/1‑140 (MQ=255)

                                                                                                      ttttAACTTTCTGTCAACAGGAATTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTcg                                  >  2:3429056/1‑139 (MQ=255)

                                                                                                       tttAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGCTGCTGTCAg                                 <  2:2142100/140‑1 (MQ=255)

                                                                                                       tttAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAg                                 <  2:617173/140‑1 (MQ=255)

                                                                                                        ttAACTTTCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAga                                >  1:4944878/1‑139 (MQ=255)

                                                                                                        ttAACTTTCTGGCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCGGGGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAgt                                >  1:4636599/1‑140 (MQ=255)

                                                                                                              ttCTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCt                          >  2:1790325/1‑140 (MQ=255)

                                                                                                                cTGTCAACAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGa                        >  1:1349846/1‑140 (MQ=255)

                                                                                                                     aaCAGGTTTTGCAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAACGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTa                   <  2:2565165/140‑1 (MQ=255)

                                                                                                                       cAGGTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACt                 <  2:2325901/140‑1 (MQ=255)

                                                                                                                          gTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt              >  2:5124418/1‑140 (MQ=255)

                                                                                                                          gTTTTGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGAt              <  2:1576817/140‑1 (MQ=255)

                                                                                                                             ttGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTAt           >  2:4577127/1‑140 (MQ=255)

                                                                                                                             ttGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTAt           >  1:4764968/1‑140 (MQ=255)

                                                                                                                              tGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc          <  1:4187618/140‑1 (MQ=255)

                                                                                                                              tGTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATc          <  1:3200858/140‑1 (MQ=255)

                                                                                                                               gTAATGGTCACT‑AAACAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAGAAGGTTTCGATGATGTCAGTGTTGCTGAAGTTACTGATTATCt         <  1:2018930/140‑1 (MQ=255)

                                                                                                                               gTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt         <  2:2482391/140‑1 (MQ=255)

                                                                                                                               gTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt         <  1:5263433/140‑1 (MQ=255)

                                                                                                                               gTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt         <  2:1860961/140‑1 (MQ=255)

                                                                                                                               gTAATGGTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCt         <  1:4469118/140‑1 (MQ=255)

                                                                                                                                    ggTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTa    >  1:1695748/1‑140 (MQ=255)

                                                                                                                                    ggTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTa    >  1:3600725/1‑140 (MQ=255)

                                                                                                                                    ggTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTa    >  2:1485179/1‑140 (MQ=255)

                                                                                                                                    ggTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATGATCTTGGTa    >  1:5045948/1‑140 (MQ=255)

                                                                                                                                    ggTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGACCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTa    >  2:4999940/1‑140 (MQ=255)

                                                                                                                                     gTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAACGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTAt   >  2:4067167/1‑140 (MQ=255)

                                                                                                                                     gTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTAt   >  2:363198/1‑140 (MQ=255)

                                                                                                                                     gTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTAt   >  1:1603745/1‑140 (MQ=255)

                                                                                                                                     gTCACT‑AAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTAt   >  2:939351/1‑140 (MQ=255)

GGCACCGATACCACGACTGCCACCGAGGATGAGAACTGTCTTACCTGTAAAAGCGCCCATAAGGACTCCTTGATTTATTATGTAACATGCATTACAAAACTGTTTTAACTTTCTGTCAACAGGTTTTGTAATGGTCACTAAAAAAACAATCTCGCGTTCCAGGTCGTCCCAGACGTTTCGCTCCTGAGCAGGCAATCTCTGCGGCAAAAGTGCTTTTTCACCAAAAAGGTTTCGATGCTGTCAGTGTTGCTGAAGTTACTGATTATCTTGGTATT  >  NZ_CP025268/4475918‑4476192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`