BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,013,520	+G	pseudogene (463/542 nt)	zapC →	cell division protein ZapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,013,518	1	.	G	97.1%	977.7 / 19.7	306	pseudogene (461/542 nt)	zapC	cell division protein ZapC
Reads supporting (aligned to +/- strand): ref base . (8/1); new base G (162/135); total (170/136)
Fisher's exact test for biased strand distribution p-value = 4.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 316 total aligned reads displayed.

TGCCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TT‑G‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGC  >  NZ_CP025268/1013380‑1013651

  ccGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TT‑g‑gg                                                                                                                                    <  1:3639652/140‑2 (MQ=255)

     ggCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TT‑g‑ggcga                                                                                                                                 <  2:3943744/140‑5 (MQ=255)

     ggCGATGCGGCCTGCGTTTGGTTAAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGACGCACTATGCCTGCTGGCACAACCCTGCGCTGTTATTGCGGGCCGCGCGATGCA‑C‑TT‑g‑ggcga                                                                                                                                 >  2:3548555/1‑136 (MQ=255)

      gCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TT‑g‑ggcgac                                                                                                                                >  2:3331824/1‑135 (MQ=255)

      gCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TT‑g‑ggcgac                                                                                                                                >  2:3766291/1‑135 (MQ=255)

                 tGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAAt                                                                                                                      >  1:1483147/1‑140 (MQ=255)

                    gTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCAt                                                                                                                   <  2:274132/140‑1 (MQ=255)

                    gTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCAt                                                                                                                   <  1:1224963/140‑1 (MQ=255)

                    gTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCAt                                                                                                                   <  1:770883/140‑1 (MQ=255)

                          ttGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGa                                                                                                             <  2:3437559/140‑1 (MQ=255)

                             aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAGAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGGCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAg                                                                                                          >  2:4405969/1‑140 (MQ=255)

                             aGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAg                                                                                                          >  2:2296979/1‑140 (MQ=255)

                                       aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  2:5597364/140‑1 (MQ=255)

                                       aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  2:3213407/140‑1 (MQ=255)

                                       aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  1:624599/140‑1 (MQ=255)

                                       aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  1:1664635/140‑1 (MQ=255)

                                       aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  2:5047112/140‑1 (MQ=255)

                                       aCGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  1:1346962/140‑1 (MQ=255)

                                       aCGAGCAGGTCAATTTGCGGGTCGTTGAATCGGGCGAAAATGGCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                                                                                <  2:3915844/140‑1 (MQ=255)

                                         gAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCa                                                                                              >  1:5049685/1‑140 (MQ=255)

                                         gAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCa                                                                                              >  2:2605605/1‑140 (MQ=255)

                                           gCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                                                                            <  2:2681742/140‑1 (MQ=255)

                                           gCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAgg                                                                                            <  2:4505602/140‑1 (MQ=255)

                                            cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:4936011/1‑140 (MQ=255)

                                            cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  2:2546121/1‑140 (MQ=255)

                                            cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:3134386/1‑140 (MQ=255)

                                            cAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:980324/1‑140 (MQ=255)

                                            cAGGTCAATTTGCTGGTCGTCGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGt                                                                                           >  1:355319/1‑140 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGG‑TG‑G‑GCGACGCCATTAAAATGATGGACGACAGGCTGAAACCGTAgggtgg                                                                                        >  2:3175139/1‑136 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                        >  2:1549601/1‑140 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                        >  1:536587/1‑140 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                        >  1:578189/1‑140 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                        >  1:705624/1‑140 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                        >  1:1952457/1‑140 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                        >  1:4300016/1‑140 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                                                                        >  1:3090725/1‑140 (MQ=255)

                                               gTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATCGCGGGTCGCGCGATCCA‑GCTC‑G‑GCGACGCCATTAACATCATGCACCACACGCCGCAACCGCAGGGTaa                                                                                        >  2:2381072/1‑140 (MQ=255)

                                                      tGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  1:2687421/140‑1 (MQ=255)

                                                      tGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCGGCTGGCACAACCCTCCGTTGTTATTGCGGGTCGCGCGATTCA‑G‑TTGG‑GCGACGCCATTAAAACCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGAc                                                                                 <  2:2138002/140‑1 (MQ=255)

                                                         tGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGc                                                                              >  1:1136258/1‑140 (MQ=255)

                                                         tGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGc                                                                              >  1:2739999/1‑140 (MQ=255)

                                                                 gAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCt                                                                      <  2:5001767/140‑1 (MQ=255)

                                                                     cTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TT‑GCGCGACGCCATTAAAATCATGAACGACAGGCCGAAACCCCAGGTTAATGTTGACAGCTTCAGCCTCGaa                                                                  >  2:4690035/1‑140 (MQ=255)

                                                                      tGGAGAAAATGACGCAATACGCATGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGAGCGATGAC‑G‑TTGG‑GCGAAGCCATTAAAATCATGCAAGCCAGGCTGAACCCGCAGGTTAATGTTGACAGCTTCAGCCTCGAAc                                                                 <  2:2904360/140‑1 (MQ=255)

                                                                         cGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAgg                                                              <  1:2768554/140‑1 (MQ=255)

                                                                         cGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAgg                                                              <  2:1000346/140‑1 (MQ=255)

                                                                                       tATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGcc                                                <  2:5450417/140‑1 (MQ=255)

                                                                                         tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGCGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCTCAAGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCaa                                              <  2:3091410/140‑1 (MQ=255)

                                                                                               ctgGCACAACCCTGCGTTTTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATGGACATCATGAACGACAGGCTGAAGCCGCACGTTAATGTTGGCAGCTTCAGCCTCGACCAGGCCCTCTAACTCGCCAACCTaaa                                        >  2:2987025/1‑139 (MQ=255)

                                                                                               ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAg                                        >  2:941538/1‑140 (MQ=255)

                                                                                               ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAg                                        >  2:5102124/1‑140 (MQ=255)

                                                                                               ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAg                                        >  2:4616507/1‑140 (MQ=255)

                                                                                                 ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTg                                      <  2:1262459/140‑1 (MQ=255)

                                                                                                 ggCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTg                                      <  2:1621245/140‑1 (MQ=255)

                                                                                                   cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCC‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACCGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    >  1:513522/1‑140 (MQ=255)

                                                                                                   cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACATGCAGTCTAACGCGCGAACTTAAGTGCa                                    >  1:301240/1‑140 (MQ=255)

                                                                                                   cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    >  2:3674822/1‑140 (MQ=255)

                                                                                                   cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    <  2:5339343/140‑1 (MQ=255)

                                                                                                   cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    >  1:3835298/1‑140 (MQ=255)

                                                                                                   cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    >  1:3447189/1‑140 (MQ=255)

                                                                                                   cacaACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCa                                    >  1:3296039/1‑140 (MQ=255)

                                                                                                     caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  1:1638778/140‑1 (MQ=255)

                                                                                                     caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  1:1781045/140‑1 (MQ=255)

                                                                                                     caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  1:2374103/140‑1 (MQ=255)

                                                                                                     caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  1:4794909/140‑1 (MQ=255)

                                                                                                     caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  >  1:3511503/1‑140 (MQ=255)

                                                                                                     caACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGt                                  <  2:1694958/140‑1 (MQ=255)

                                                                                                        cgcTGGGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCCTTAAAATCCTGAACGACAGGCTGAAACCGCAGGTTCATGCTGACAGCTTCTGCTTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                               >  2:890412/3‑140 (MQ=255)

                                                                                                        cccTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                               <  1:1045678/140‑1 (MQ=255)

                                                                                                        cccTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCtt                               <  1:2179219/140‑1 (MQ=255)

                                                                                                           tGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGAGCAGTCTTCgg                            >  1:3324604/1‑140 (MQ=255)

                                                                                                             cGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                          <  2:4557697/140‑1 (MQ=255)

                                                                                                             cGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                          <  2:4320606/140‑1 (MQ=255)

                                                                                                             cGTTGTTATTGCAGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTa                          <  1:4786606/140‑1 (MQ=255)

                                                                                                              gttgttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTac                         <  1:3760828/140‑1 (MQ=255)

                                                                                                               ttgttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTaca                        <  2:1167751/140‑1 (MQ=255)

                                                                                                                tgttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTacac                       >  1:5185701/1‑140 (MQ=255)

                                                                                                                  ttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  1:5399369/1‑140 (MQ=255)

                                                                                                                  ttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  1:2008987/1‑140 (MQ=255)

                                                                                                                  ttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  2:4790327/1‑140 (MQ=255)

                                                                                                                  ttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  2:2178895/1‑140 (MQ=255)

                                                                                                                  ttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  2:2806726/1‑140 (MQ=255)

                                                                                                                  ttATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAg                     >  1:3381271/1‑140 (MQ=255)

                                                                                                                   tATTGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGc                    <  2:790902/140‑1 (MQ=255)

                                                                                                                     ttGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                  <  2:1877572/140‑1 (MQ=255)

                                                                                                                     ttGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                  <  2:4168860/140‑1 (MQ=255)

                                                                                                                     ttGCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGGTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTa                  <  2:1974327/140‑1 (MQ=255)

                                                                                                                       gCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACa                >  1:3189436/1‑140 (MQ=255)

                                                                                                                       gCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACa                >  1:5520068/1‑140 (MQ=255)

                                                                                                                       gCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACa                >  1:2339792/1‑140 (MQ=255)

                                                                                                                       gCGGGTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCGCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACa                >  1:5005102/1‑140 (MQ=255)

                                                                                                                       gCGGGTCGCGCGAAGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACa                <  2:4865041/140‑1 (MQ=255)

                                                                                                                          ggTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGTAGGGTAATGTTGACAGCTTCAGGCGCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCGACAGCa             >  2:2073082/1‑140 (MQ=255)

                                                                                                                          ggTCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             >  1:2551409/1‑140 (MQ=255)

                                                                                                                          ggTCGCGCGATGCA‑G‑TTGG‑GCGAAGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCa             <  1:483441/140‑1 (MQ=255)

                                                                                                                            tCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa           <  2:3581974/140‑1 (MQ=255)

                                                                                                                            tCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa           >  1:2916360/1‑140 (MQ=255)

                                                                                                                            tCGCGCGATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCaaa           >  1:4358598/1‑140 (MQ=255)

                                                                                                                               cgcgATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGaa        >  1:4483317/1‑140 (MQ=255)

                                                                                                                                 cgATGCA‑G‑TTGG‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATg      <  1:250889/140‑1 (MQ=255)

TGCCGGGCGATGCGGCCTGCGTTTGGTTGAGCGATACTCACGAGCAGGTCAATTTGCTGGTCGTTGAATCTGGCGAAAATGCCGCACTATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCA‑G‑TT‑G‑GCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTTTAACGCGCCAACTTAAGTGCAGTCTTCGGTACACAGCTACAGCAAAGAATGGTGC  >  NZ_CP025268/1013380‑1013651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`