BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,146,507	+T	pseudogene (1028/1643 nt)	flgK →	flagellar hook‑associated protein FlgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,146,507	1	.	T	98.3%	1081.3 / 6.8	305	pseudogene (1028/1643 nt)	flgK	flagellar hook‑associated protein FlgK
Reads supporting (aligned to +/- strand): ref base . (1/4); new base T (128/169); total (129/176)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

ACGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAT  >  NZ_CP025268/1146368‑1146639

 cGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGAGGCTAACGGCGAGGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gt                                                                                                                                      >  2:5476633/1‑139 (MQ=255)

  gCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtg                                                                                                                                     <  1:4369253/140‑3 (MQ=255)

  gCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtg                                                                                                                                     >  2:3222447/1‑138 (MQ=255)

    ttGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgac                                                                                                                                   >  1:641519/1‑136 (MQ=255)

     tGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgacg                                                                                                                                  >  2:2979369/1‑135 (MQ=255)

     tGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgacg                                                                                                                                  >  1:5115136/1‑135 (MQ=255)

     tGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑g‑gtgacg                                                                                                                                  >  2:288619/1‑135 (MQ=255)

       gACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACAGCCAACACAAAGCCGGATGAGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATGGGTAAGCCCGCGGTTCTGCAAAACACGAAAATCAAA‑G‑GTGACGtt                                                                                                                                >  2:4843585/1‑140 (MQ=255)

       gACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGtt                                                                                                                                >  2:2851989/1‑140 (MQ=255)

        aCAACCGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGACTTGACGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTg                                                                                                                               <  2:659628/140‑1 (MQ=255)

         cAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGc                                                                                                                              >  1:1930236/1‑140 (MQ=255)

          aaCTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCg                                                                                                                             <  1:1551518/140‑1 (MQ=255)

            cgggCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCGTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:3071499/138‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGGGTCTTTCAACACCCAACACCAAGCCGGATTTGATGCTCACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCGGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCAAt                                                                                                                           >  2:339441/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCATCACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:5188659/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAACGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:3556587/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:2673250/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:852834/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  1:4681039/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  2:1005666/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  1:4025046/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           >  2:3271191/1‑140 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  1:1636299/140‑1 (MQ=255)

            cTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  1:2307018/140‑1 (MQ=255)

                  cTGGCATTTGCCGAGGCTTTCAACCCCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATCTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAGAAACAAA‑G‑GTGACGTTGCGATCGGTGc                                                                                                                     <  2:2389116/140‑1 (MQ=255)

                    ggCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCa                                                                                                                   <  1:3835946/140‑1 (MQ=255)

                      cATTCGACGCGGCTTTCCACACACAACACAACGCCGGATTTGCTGCTAACGGCGATGCCGGTGAAGATTTCTTTGCCATCGGTAAGCCCGCGGTTCTGCAAACCACGAACAACAAA‑G‑GTGACGTTGCGATCGGTGCCACg                                                                                                                 <  2:3051323/140‑1 (MQ=255)

                       aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  1:4609048/140‑1 (MQ=255)

                       aTTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACgg                                                                                                                <  1:4072522/140‑1 (MQ=255)

                       aTTTGCCCAGGCTTTCAACACCCAACGCAAAGCCGGACTTGATGCTAACGGCGATTCCGGTGAACATTTCTTTGCTATCGGTGAGCCCGCGGTTCTGCAAAACACGACACTCAAA‑G‑GCGACGTTGCGATCGGTGCCACgg                                                                                                                <  2:1760813/140‑1 (MQ=255)

                           gCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAAc                                                                                                            >  1:2658488/1‑140 (MQ=255)

                           gCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAAc                                                                                                            >  1:1864262/1‑140 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        <  2:1541260/140‑1 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        >  2:4753817/1‑140 (MQ=255)

                               aGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAt                                                                                                        >  1:3341717/1‑140 (MQ=255)

                                 gCTTTCCACCCCCAACACACCGCCGGCTTCGACGCTAACGGCGATGCCGCTGAAGATTACTTTGCTCTCGATAATCCCCCGGTTCTTCAAAACCCGAAAAACACA‑G‑GTGACGTCGCGATCGGTGCCACGGTAACTGATGc                                                                                                      <  2:3122338/140‑1 (MQ=255)

                                   tttCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTGAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCt                                                                                                    <  2:1323097/140‑1 (MQ=255)

                                   tttCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCt                                                                                                    <  1:5247116/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  1:2925249/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 >  1:3042203/1‑140 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  2:5325758/140‑1 (MQ=255)

                                      cAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcg                                                                                                 <  1:4945000/140‑1 (MQ=255)

                                        acacCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCcgcg                                                                                               <  2:1393021/140‑1 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGGAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            >  2:1888625/1‑140 (MQ=255)

                                           cccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTa                                                                                            <  2:970743/140‑1 (MQ=255)

                                            ccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTAc                                                                                           >  1:820101/1‑140 (MQ=255)

                                            ccAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTAc                                                                                           >  1:448133/1‑140 (MQ=255)

                                             cAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACt                                                                                          >  1:1990234/1‑140 (MQ=255)

                                               acacAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGAt                                                                                                                           <  1:2978402/105‑1 (MQ=255)

                                               acacAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTgg                                                                                        >  2:4466148/1‑140 (MQ=255)

                                                cacaAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGc                                                                                       >  1:2494932/1‑140 (MQ=255)

                                                  caAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGa                                                                                     <  1:3698334/140‑1 (MQ=255)

                                                    aaGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACa                                                                                   >  1:125641/1‑140 (MQ=255)

                                                          gATTTGATGCTAACGGCGATGCTGGTGAAGATTTCGTAGCTAGCGGTAAGCCAGCGGTGCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAGCTGATGTCTCCGCAGTACTAGCGACAGGTTaa                                                                             >  2:710412/1‑139 (MQ=255)

                                                          gATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTAc                                                                             >  1:1518126/1‑140 (MQ=255)

                                                          gATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACGGGAGACAGATTAc                                                                             >  2:4093523/1‑140 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:4204380/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:1432633/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:4263329/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  2:3665152/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  <  1:1275786/140‑1 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGtt                                                                  >  1:2358686/1‑140 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGGACTGGCGACAGATGACAAAATCTCGtt                                                                  >  1:1172070/1‑140 (MQ=255)

                                                                     aaCGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCGCCGCGGTACTGGCGACAGATTACAAAATCTCggt                                                                  <  2:2206226/140‑3 (MQ=255)

                                                                             tGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGataata                                                          >  1:2126819/1‑140 (MQ=255)

                                                                              gCCGGTGAAGATTGCTTTGCTATCGGTAAGCCCGCGGTTATGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCAGCGGTATTGGCGACAGATTACAAAATCTCGTTCGAtaagaa                                                         >  2:4424665/1‑140 (MQ=255)

                                                                                     aaGATTTAGTTGAGATCGGTAAGCGCGCGGTTCTGCAAAGCACGAAAATCAGA‑G‑GTGACTTTGCGATCGGTGCGACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTgg                                                  <  2:2813782/140‑1 (MQ=255)

                                                                                      aGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAATATCTCGTTCGATAATAATCAGTGGc                                                 >  2:3702832/1‑140 (MQ=255)

                                                                                      aGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGc                                                 <  2:2467455/140‑1 (MQ=255)

                                                                                      aGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGc                                                 <  1:2772839/140‑1 (MQ=255)

                                                                                       gATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCa                                                <  1:343925/140‑1 (MQ=255)

                                                                                       gATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCa                                                <  2:508983/140‑1 (MQ=255)

                                                                                            ctctGCTATCGGCAAGACCGCGGTTCTGCAAAACCCGAAAACCAAA‑G‑GTGACGTTGGGATCGGTGACACGGTAACTGATGCCTCCGCGGTACTGGCGACAGCTTACAAAATCTCGTTCGATAATAATCAGTGGCACGtaa                                           <  2:600531/137‑3 (MQ=255)

                                                                                               tGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACTAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCAccc                                        <  2:5507655/140‑1 (MQ=255)

                                                                                                  tATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGcc                                     >  1:1014588/1‑140 (MQ=255)

                                                                                                   aTCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCt                                    >  1:2870839/1‑140 (MQ=255)

                                                                                                    tCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCtt                                   <  1:987102/140‑1 (MQ=255)

                                                                                                       gTAAGCCCGCGGTTCAGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCAGGGTAACTGATGCCTCCGCGGTACTGGTGACAGAGTACAAAATCTCGTTCGATAATAATGAGTGGCAGGTCACCCGCGTTGcg                                >  2:3617435/1‑139 (MQ=255)

                                                                                                         aaGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAg                              <  1:3534067/140‑1 (MQ=255)

                                                                                                          aGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGc                             <  2:5581294/140‑1 (MQ=255)

                                                                                                            cccGCGGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCaa                           >  2:1085691/1‑140 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACGCGAGAAACAAA‑G‑GTGACGCTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:3876893/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACCAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGAGGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  2:4314741/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        >  1:3525780/1‑140 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:3123569/140‑1 (MQ=255)

                                                                                                               gcgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAc                        <  1:2526272/140‑1 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  1:1305633/1‑140 (MQ=255)

                                                                                                                cgGTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATAcc                       >  1:4422151/1‑140 (MQ=255)

                                                                                                                 ggTTCTGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCa                      <  1:3222447/140‑1 (MQ=255)

                                                                                                                      tGCAAAACACGAAAAACAAA‑G‑GTGACGTTGCAATCGGTGCCACTGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGATCACGCGCCTTGCCAACAATACCACtttt                 >  2:4263793/1‑140 (MQ=255)

                                                                                                                       gCAAAACACGAAAAACAAA‑GTT‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTa                <  2:501527/140‑1 (MQ=255)

                                                                                                                          aaaCACGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACgg             <  2:1584787/140‑1 (MQ=255)

                                                                                                                             cacGAAAAACAAAGG‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGCCAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          <  2:2021666/140‑1 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCATTTTTACGGTGa          >  1:3141597/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  1:2618655/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:2899017/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:226468/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGa          >  2:1973784/1‑140 (MQ=255)

                                                                                                                             cacGAAAAACAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTCTACGGTGa          >  1:4191434/1‑140 (MQ=255)

                                                                                                                                  aaaaCAAA‑G‑GTGACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCg     <  1:541980/140‑1 (MQ=255)

ACGCTTGGACAACTGGCGCTGGCATTTGCCGAGGCTTTCAACACCCAACACAAAGCCGGATTTGATGCTAACGGCGATGCCGGTGAAGATTTCTTTGCTATCGGTAAGCCCGCGGTTCTGCAAAACACGAAAAACAAA‑G‑G‑GACGTTGCGATCGGTGCCACGGTAACTGATGCCTCCGCGGTACTGGCGACAGATTACAAAATCTCGTTCGATAATAATCAGTGGCAGGTCACCCGCCTTGCCAGCAATACCACTTTTACGGTGACGCCGGAT  >  NZ_CP025268/1146368‑1146639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`