BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,175,450	+G	pseudogene (483/632 nt)	comR ←	TetR family copper‑responsive transcriptional repressor ComR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,175,448	1	.	G	97.3%	855.9 / 13.4	260	pseudogene (485/632 nt)	comR	TetR family copper‑responsive transcriptional repressor ComR
Reads supporting (aligned to +/- strand): ref base . (2/5); new base G (106/146); total (108/152)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

TTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑G‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAG  >  NZ_CP025268/1175313‑1175582

ttCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATCATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑g‑gga                                                                                                                                      >  2:4485985/1‑138 (MQ=255)

ttCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑g‑gga                                                                                                                                      >  2:767893/1‑138 (MQ=255)

ttCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑g‑gga                                                                                                                                      >  2:5214859/1‑138 (MQ=255)

ttCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑g‑gga                                                                                                                                      >  1:3541975/1‑138 (MQ=255)

          aaaCGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCcgcg                                                                                                                           <  1:4655322/140‑1 (MQ=255)

          aaaCGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCcgcg                                                                                                                           >  1:3748075/1‑140 (MQ=255)

          aaaCGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGAGCGACATCCCCTGAATAATACAATTACGGACTTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCcgcg                                                                                                                           >  2:912290/1‑140 (MQ=255)

                     cGTTCCGGCAATCTGCATTAGTTTTCCCAGCGATACACCATCGCGTTCCCTGAACGACAGCACCTGAATAACACAATTAAGGAAATCTGCCACATGTGTCACGTCACACTGGGGC‑G‑GTAT‑TTCCCCGCGCGCTTGTCGtt                                                                                                                <  2:1853173/140‑1 (MQ=255)

                     cGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGtt                                                                                                                <  1:976989/140‑1 (MQ=255)

                     cGTTCCGGCAATCTGCATCAGTTTTTCCAGCGAGGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGtt                                                                                                                <  2:1251339/140‑1 (MQ=255)

                       ttCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGa                                                                                                              >  1:460478/1‑140 (MQ=255)

                        tCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGAc                                                                                                             >  1:2682208/1‑140 (MQ=255)

                        tCCGGAAATCTGCATAAGTCTTTCCAGCGATGCACCTTCGCGTCCGCTGATCGACATCCCCTGAATAATAGAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGTGCTTGTCGTTGAc                                                                                                             <  2:4054206/140‑1 (MQ=255)

                          cGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCTACATCCCCTTAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACAt                                                                                                           >  1:5035266/1‑140 (MQ=255)

                             cAATCTGCTTCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCTCGCTTGTCGTTGACATaaa                                                                                                        <  2:4626465/140‑1 (MQ=255)

                             cAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATaaa                                                                                                        <  2:2142209/140‑1 (MQ=255)

                             cAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATaaa                                                                                                        <  1:1761054/140‑1 (MQ=255)

                               aTCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATaaaaa                                                                                                      <  1:2919877/140‑1 (MQ=255)

                               aTCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGATGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATaaaaa                                                                                                      <  2:2294389/140‑1 (MQ=255)

                                            tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                         <  1:4176445/140‑1 (MQ=255)

                                            tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                         <  1:4136569/140‑1 (MQ=255)

                                            tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                         <  2:2601329/140‑1 (MQ=255)

                                            tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                         <  2:1659552/140‑1 (MQ=255)

                                            tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                         <  1:2625353/140‑1 (MQ=255)

                                            tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                         <  1:2390453/140‑1 (MQ=255)

                                            tttCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTg                                                                                         <  1:2127251/140‑1 (MQ=255)

                                              tCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACCATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTgcg                                                                                       >  2:330941/1‑140 (MQ=255)

                                              tCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTgcg                                                                                       >  2:1708039/1‑140 (MQ=255)

                                              tCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTgcg                                                                                       <  2:1965338/140‑1 (MQ=255)

                                                 aGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGctc                                                                                    <  2:3482533/140‑1 (MQ=255)

                                                 aGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGctc                                                                                    <  1:1015449/140‑1 (MQ=255)

                                                           cTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCa                                                                          <  2:3278097/140‑1 (MQ=255)

                                                                  tGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTg                                                                   >  1:1247571/1‑140 (MQ=255)

                                                                  tGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTg                                                                   >  1:3406499/1‑140 (MQ=255)

                                                                    cgcTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGAt                                                                 >  2:4530397/1‑140 (MQ=255)

                                                                      cTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑G‑GCAT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGAttt                                                               <  2:5156994/140‑1 (MQ=255)

                                                                         aTCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCaa                                                            >  2:5257515/1‑140 (MQ=255)

                                                                             aCATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTa                                                        <  1:1996730/140‑1 (MQ=255)

                                                                             aCATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTa                                                        <  1:3278472/140‑1 (MQ=255)

                                                                                tCCCCTGAATAATACAATTAAGGAATGCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTg                                                     <  2:2987826/140‑1 (MQ=255)

                                                                                 ccccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTgg                                                    >  2:4008458/1‑140 (MQ=255)

                                                                                   ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑CT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCg                                                  >  1:2835609/1‑140 (MQ=255)

                                                                                   ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCg                                                  >  2:1562990/1‑140 (MQ=255)

                                                                                   ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCg                                                  >  1:3217025/1‑140 (MQ=255)

                                                                                     tGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAACCTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGat                                                <  2:1452344/140‑1 (MQ=255)

                                                                                     tGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCTCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGat                                                <  1:4821934/140‑1 (MQ=255)

                                                                                     tGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGat                                                <  1:1880589/140‑1 (MQ=255)

                                                                                     tGAACAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AC‑TTCCCCCCGCGCTTGTCCTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTCGCGat                                                <  2:3154254/140‑1 (MQ=255)

                                                                                       aataatACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTTCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGatat                                              >  1:1638078/1‑140 (MQ=255)

                                                                                       aataatACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGatat                                              <  1:4966989/140‑1 (MQ=255)

                                                                                       aataatACAAGTAAGGACTGCTGGCACAGGTGTCACGTCACAGTGGGGC‑G‑G‑GTGTCCCCCGCGCGCTTGTCGTCGACATAAAAACTGCTGCAAAGTGCGCTCTTCCATCGCATGGCGTGATTTCAACGTATTGGCGatat                                              <  2:1341948/140‑1 (MQ=255)

                                                                                         taataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTa                                                        >  2:3606244/1‑128 (MQ=255)

                                                                                         taataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTa                                                        <  1:3606691/128‑1 (MQ=255)

                                                                                         taataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTa                                                        >  2:2161857/1‑128 (MQ=255)

                                                                                           ataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATCTCTcc                                          >  2:4919745/1‑140 (MQ=255)

                                                                                           ataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTcc                                          <  2:1204409/140‑1 (MQ=255)

                                                                                           ataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTcc                                          <  2:2342426/140‑1 (MQ=255)

                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTGACGTCATAGTGGGGCGG‑G‑AT‑TTCCCCGCGTGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATCTCGCCt                                         >  2:2022927/1‑140 (MQ=255)

                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGTCGATATCTCCt                                         >  1:1199043/1‑140 (MQ=255)

                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                         >  2:5396052/1‑140 (MQ=255)

                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                         >  2:5500550/1‑140 (MQ=255)

                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                         >  1:2290692/1‑140 (MQ=255)

                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                         >  1:659152/1‑140 (MQ=255)

                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                         >  1:2462600/1‑140 (MQ=255)

                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCt                                         >  1:2985080/1‑140 (MQ=255)

                                                                                             aCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCg                                                                     <  1:4762157/111‑1 (MQ=255)

                                                                                             aCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTg                                        <  1:448426/140‑1 (MQ=255)

                                                                                              cAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGa                                       <  2:1386691/140‑1 (MQ=255)

                                                                                                aTTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAAt                                     <  2:2912861/140‑1 (MQ=255)

                                                                                                 ttAAGTAATTCTGCCAGCTGTGTCACGTCACACTGGGGCGG‑G‑AT‑TTCCCCGCGCCCTTGTCGTTGACATAAAAACTGCGGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCTACGTATTGGCGATATCTCCTGAATc                                    <  2:5012127/140‑1 (MQ=255)

                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                    >  1:5203371/1‑140 (MQ=255)

                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                    >  2:4235019/1‑140 (MQ=255)

                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                    <  2:467728/140‑1 (MQ=255)

                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                    <  2:1870855/140‑1 (MQ=255)

                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                    >  1:1392284/1‑140 (MQ=255)

                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑GCG‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                                    <  1:708458/140‑1 (MQ=255)

                                                                                                  tAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCt                                   <  2:1101527/140‑1 (MQ=255)

                                                                                                  tAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCt                                   <  1:3778340/140‑1 (MQ=255)

                                                                                                  tAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCt                                   <  1:5528587/140‑1 (MQ=255)

                                                                                                      gAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTgggg                               >  1:1290631/1‑140 (MQ=255)

                                                                                                      gAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTgggg                               >  1:114971/1‑140 (MQ=255)

                                                                                                       aaTTCTGCCAGATGTGTCACGTCTCAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGga                              <  1:5131349/140‑1 (MQ=255)

                                                                                                       aaTTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGga                              <  2:3705088/140‑1 (MQ=255)

                                                                                                       aaTTCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGga                              <  1:3146125/140‑1 (MQ=255)

                                                                                                         ttCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGgaga                            >  2:1008640/1‑140 (MQ=255)

                                                                                                         ttCTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGgaga                            <  2:1333498/140‑1 (MQ=255)

                                                                                                           cTGCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAgg                          >  2:548573/1‑140 (MQ=255)

                                                                                                             gCCAGATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGggt                        <  1:4259112/140‑1 (MQ=255)

                                                                                                                 gATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg                    <  1:912605/140‑1 (MQ=255)

                                                                                                                 gATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg                    <  2:1713623/140‑1 (MQ=255)

                                                                                                                 gATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCCCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg                    <  1:4437676/140‑1 (MQ=255)

                                                                                                                 gATGTGTCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTCGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg                    >  2:4411658/1‑140 (MQ=255)

                                                                                                                    gtgtCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAg                 >  2:2739501/1‑140 (MQ=255)

                                                                                                                    gtgtCACGTCACAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAg                 <  2:2462600/140‑1 (MQ=255)

                                                                                                                      gtcacgtcacAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACCGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgtt               >  2:3612138/1‑140 (MQ=255)

                                                                                                                        cacgtcacAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgttgt             >  1:783479/1‑140 (MQ=255)

                                                                                                                         acgtcacAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAgttgtt            <  1:5247843/140‑1 (MQ=255)

                                                                                                                         acgtcacAGTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTCGGGAGAGGGTGGTGCAgttgtt            >  2:3099979/1‑140 (MQ=255)

                                                                                                                                 gTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATACAACCTGCTGCAACGTGCGCTCTTGCATTGCATGGCGTGATCTCAACGTATTGGCGATAGCGCCTGAATCTTGGGAGAGGGTGGTGCAGTTGTTGACCATGa    <  2:1781161/140‑1 (MQ=255)

                                                                                                                                 gTGGGGCGG‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACAGAAAAACTGCTGCAAAGTCAGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGTTATCTCCTGAATCTGGGGAGAGGGTGGTGCACTTGTTGATCATGa    >  2:4837862/1‑140 (MQ=255)

TTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGC‑G‑G‑AT‑TTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATCATGAAG  >  NZ_CP025268/1175313‑1175582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`