BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,224,095	+GA	coding (248/249 nt)	CXP41_RS06440 →	protein YmgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,224,094	1	.	A	99.3%	1157.4 / ‑1.5	304	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (1/1); new base A (183/119); total (184/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*	NZ_CP025268	1,224,094	2	.	G	99.3%	1020.1 / ‑1.4	304	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (183/118); total (184/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 323 total aligned reads displayed.

CAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑A‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCC  >  NZ_CP025268/1223956‑1224224

||

 aGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑ag                                                                                                                                    >  1:5037028/1‑139 (MQ=255)

   gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaa                                                                                                                                  >  2:779415/1‑137 (MQ=255)

   gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaa                                                                                                                                  >  1:3272269/1‑137 (MQ=255)

    cTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaac                                                                                                                                 <  1:313297/140‑5 (MQ=255)

    cTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaac                                                                                                                                 <  1:4139296/140‑5 (MQ=255)

    cTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaac                                                                                                                                 <  2:582764/140‑5 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTa                                                                                                                          <  2:3940082/140‑1 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTa                                                                                                                          <  1:2146129/140‑1 (MQ=255)

           cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTa                                                                                                                          <  1:3143611/140‑1 (MQ=255)

             tGCGTTTACAAGATGAAACCAGTGTGTTTCTCTCTGATCTCTATCTGCAAATATTTCAGGACATTTAAATGCATAGGACAACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCACTTAGA‑‑ACTTGAGTAAg                                                                                                                        >  2:3351704/1‑140 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAg                                                                                                                        >  2:388136/1‑140 (MQ=255)

             tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAg                                                                                                                        >  1:1238218/1‑140 (MQ=255)

                      aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGaa                                                                                                               <  2:1551590/140‑1 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                       <  2:2877477/140‑1 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                       <  2:2545101/140‑1 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                       <  2:1634589/140‑1 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                       <  1:2911517/140‑1 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                       <  2:3256267/140‑1 (MQ=255)

                              aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                       <  2:4912494/140‑1 (MQ=255)

                                cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                     <  1:2415615/140‑1 (MQ=255)

                                cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                     <  1:1918187/140‑1 (MQ=255)

                                   tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                  >  2:2658972/1‑140 (MQ=255)

                                   tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                  >  2:1442698/1‑140 (MQ=255)

                                   tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                  >  2:552483/1‑140 (MQ=255)

                                        ttctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                  >  2:2018575/1‑135 (MQ=255)

                                        ttctttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCa                                                                                             >  1:675444/1‑140 (MQ=255)

                                        ttctttatGATCTCTATCTGCAACTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGACTAAGGTTTTAGAATAAACATCAGCTGTATCa                                                                                             <  2:3173325/140‑1 (MQ=255)

                                             tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCAta                                                                                        >  1:4952726/1‑139 (MQ=255)

                                             tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                        >  1:5558879/1‑140 (MQ=255)

                                             tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATAACATTATTAATGACAAGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                        >  2:1116786/1‑140 (MQ=255)

                                                      tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                               >  1:2572839/1‑140 (MQ=255)

                                                      tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                               <  2:816830/140‑1 (MQ=255)

                                                      tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATCCCAGGGAACCCGTGCTCTCTTAGA‑‑ACTTGCGTACGGTTTTAGAATAACCATCAGCTGTATCACCATGCTGATGCaa                                                                               <  2:4244233/140‑1 (MQ=255)

                                                           gCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGa                                                                          >  1:3430634/1‑140 (MQ=255)

                                                            cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                         <  1:4235105/140‑1 (MQ=255)

                                                            cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                         <  1:4377045/140‑1 (MQ=255)

                                                                  ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTc                                                                   <  1:779415/140‑1 (MQ=255)

                                                                         aCATTGAAATGCATAAGACTACAATAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGtt                                                            <  1:4278616/140‑1 (MQ=255)

                                                                            ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                         <  1:1464281/140‑1 (MQ=255)

                                                                            ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                         <  1:178116/140‑1 (MQ=255)

                                                                            ttGAAATGCATAAGACTACACTAACGGATATCATGATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                         <  1:1325921/140‑1 (MQ=255)

                                                                             tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                        >  1:1013301/1‑140 (MQ=255)

                                                                             tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                        >  1:2094966/1‑140 (MQ=255)

                                                                              gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                       <  2:2320971/140‑1 (MQ=255)

                                                                              gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                       <  2:4266794/140‑1 (MQ=255)

                                                                              gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                       <  2:122935/140‑1 (MQ=255)

                                                                                  tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                   >  1:4969095/1‑140 (MQ=255)

                                                                                  tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                   >  2:2412116/1‑140 (MQ=255)

                                                                                  tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                   >  1:1563542/1‑140 (MQ=255)

                                                                                  tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                   >  2:5330810/1‑140 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                 >  1:4041076/1‑140 (MQ=255)

                                                                                    cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                 >  2:1894028/1‑140 (MQ=255)

                                                                                      tAAGACTACACTAACGGATATCATTAGTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACGTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTGCttatt                                               >  1:572826/1‑140 (MQ=255)

                                                                                        aGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTtattat                                             <  1:1360840/140‑1 (MQ=255)

                                                                                        aGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTtattat                                             <  1:3916514/140‑1 (MQ=255)

                                                                                            tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                         <  2:4747626/140‑1 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                       >  2:1809855/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                       >  1:825060/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                       >  1:1310088/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                       >  1:2530325/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                       >  1:2683710/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                       >  1:2010795/1‑140 (MQ=255)

                                                                                              cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                       >  1:3723560/1‑140 (MQ=255)

                                                                                              cacGAACGGGTATCATTAGTAGGGACGGGGAATCCGTGTGCTCGT‑‑AGTATTAGAGTAAGGTTTTAGAATAACTATCAGCTGTATGACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATATTTTCGTATTATGTCGGc                                       >  2:4393220/1‑140 (MQ=255)

                                                                                               acTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTGTCTTATTATGTCCGCa                                      >  1:1656756/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                         >  2:4085729/1‑117 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                  >  2:1319255/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                  >  2:1365718/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                  >  2:349000/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                  >  1:4425825/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                  >  1:1342002/1‑140 (MQ=255)

                                                                                                   aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTAGTATGTCCGCAatat                                  >  2:2165287/1‑140 (MQ=255)

                                                                                                   aCGGATAACATTATTAATGACAGGGAATCCGTGATCTCTTAGA‑‑ACTTGAGTAAGGTTGTAGAATAAACATCAGCTGTATCATCATGCTGAGGCAAAGTGAGCATTTCAGGCTTTATGCTTTCTTATTATGTTCGCAatat                                  >  2:302756/1‑140 (MQ=255)

                                                                                                    cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                 >  1:2050975/1‑140 (MQ=255)

                                                                                                    cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                 >  2:1964996/1‑140 (MQ=255)

                                                                                                    cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                 >  1:3074373/1‑140 (MQ=255)

                                                                                                     ggATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCa                                >  1:4638172/1‑140 (MQ=255)

                                                                                                     ggATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCa                                >  2:2504826/1‑140 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                       >  1:3563188/1‑114 (MQ=255)

                                                                                                        tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                       <  2:4958701/114‑1 (MQ=255)

                                                                                                          tCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtgt                           <  1:3614699/140‑1 (MQ=255)

                                                                                                            attattAATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCa                         <  1:4459778/140‑1 (MQ=255)

                                                                                                             ttattaATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGCAAGAGTTTAGAATAAACATCAGCTGTATCATCATGCTGATGCAAAGTGAGCATTTTAGGCGTTATGCTTTCGTATTATGGCCGCAATATCAGTTGTCaa                        >  2:2730760/1‑140 (MQ=255)

                                                                                                               attaATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGa                      >  2:4160861/1‑140 (MQ=255)

                                                                                                                ttaATGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGaa                     >  1:1633120/1‑140 (MQ=255)

                                                                                                                   aTGACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATgg                  >  1:991396/1‑140 (MQ=255)

                                                                                                                     gACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag                >  1:1694147/1‑140 (MQ=255)

                                                                                                                     gACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag                <  2:3682694/140‑1 (MQ=255)

                                                                                                                     gACAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag                >  1:2762778/1‑140 (MQ=255)

                                                                                                                      aCAGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgaga               >  2:4634354/1‑140 (MQ=255)

                                                                                                                        aGGGAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGt             >  2:2485598/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                  <  2:5562552/53‑1 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc           >  1:4435584/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc           >  1:5100152/1‑140 (MQ=255)

                                                                                                                          ggAATCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc           >  2:935412/1‑140 (MQ=255)

                                                                                                                            aaTCCGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtctc         <  2:1217241/140‑1 (MQ=255)

                                                                                                                               ccGTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGct      <  2:5467430/140‑1 (MQ=255)

                                                                                                                               cagTGCTCTCTT‑‑ACCACCTGAGTAAGCTTCTCGAATCAACAATCCCTGTATCCCACTGATGATGCAAAGTGAGCACCTCACGCGTCATGCTTTCTTATTATGTCCGCAATAACCAGTGTCAAGAATCGCGAGTTCTCGct      <  2:4528504/138‑1 (MQ=255)

                                                                                                                                 gTGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCGTAGTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGctct    <  1:88731/140‑1 (MQ=255)

                                                                                                                                  tGCTCTCTTAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc   >  1:4829392/1‑140 (MQ=255)

||

CAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑A‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCC  >  NZ_CP025268/1223956‑1224224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`