BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,826,683	(A)_7→8	intergenic (‑247/+51)	chbB ← / ← osmE	PTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,826,676	1	.	A	96.2%	1615.6 / 44.3	442	intergenic (‑240/+58)	chbB/osmE	PTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE
Reads supporting (aligned to +/- strand): ref base . (13/4); new base A (301/124); total (314/128)
Fisher's exact test for biased strand distribution p-value = 7.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 445 total aligned reads displayed.

AAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCC‑‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826545‑1826810

aaaGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTTGCGAAGCTAATTAAGAACTCAG‑GCC‑‑aaaaa‑aaa                                                                                                                                 >  2:773263/1‑139 (MQ=255)

              tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGcc                                                                                                                    >  1:76052/1‑140 (MQ=255)

              tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGcc                                                                                                                    >  1:5064860/1‑140 (MQ=255)

              tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGcc                                                                                                                    >  1:5028137/1‑140 (MQ=255)

              tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGcc                                                                                                                    >  2:4232985/1‑140 (MQ=255)

                taGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCgg                                                                                                                  >  2:3198034/1‑140 (MQ=255)

                 aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGt                                                                                                                 >  2:4197309/1‑140 (MQ=255)

                   aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGttt                                                                                                               >  1:1051415/1‑140 (MQ=255)

                   aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGttt                                                                                                               >  2:977610/1‑140 (MQ=255)

                   aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGttt                                                                                                               >  2:3885321/1‑140 (MQ=255)

                   aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGttt                                                                                                               >  1:3638608/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAGGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:3993699/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGTCCGGTTTCCgtt                                                                                                          >  2:158801/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:4090270/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:2456528/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:682576/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:3302170/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:143539/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:4038143/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:912297/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:1615643/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:489798/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:5477162/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:1678345/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:2068960/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:444889/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:4081965/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:3873220/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:4511519/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:5280737/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:2602150/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:262965/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:2761726/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:5082154/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:499690/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:4924347/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:4698198/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:3488495/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:3560279/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:211097/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTGCCgtt                                                                                                          >  2:1553908/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCC‑‑AAAAACAACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:2624765/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACGCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:2556820/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATTGGGTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  1:1404540/1‑140 (MQ=255)

                        aGCAATAGTATTTCTTTGACCTTATAAATGGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCgtt                                                                                                          >  2:4342485/1‑140 (MQ=255)

                             tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                     >  1:4784950/1‑140 (MQ=255)

                             tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                     >  1:4992337/1‑140 (MQ=255)

                               gTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTc                                                                                                   <  1:3198034/140‑1 (MQ=255)

                                  tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                                >  1:52202/1‑140 (MQ=255)

                                       ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTc                                                                                           >  1:1347390/1‑140 (MQ=255)

                                        tGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCa                                                                                          >  2:2351429/1‑140 (MQ=255)

                                           ccTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                       <  1:2488543/140‑1 (MQ=255)

                                              tataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTCCTCAAGCCTTCAGACGaa                                                                                    >  1:5597760/1‑140 (MQ=255)

                                               ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                                   >  2:5354905/1‑140 (MQ=255)

                                               ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                                   >  2:295866/1‑140 (MQ=255)

                                                taAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAtt                                                                                  <  2:2177407/140‑1 (MQ=255)

                                                            ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATGAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                      <  2:348915/140‑1 (MQ=255)

                                                              ttGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGc                                                                    <  1:1140819/140‑1 (MQ=255)

                                                              ttGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGc                                                                    <  1:1530321/140‑1 (MQ=255)

                                                                 aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                 <  1:459017/140‑1 (MQ=255)

                                                                   cTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtgtg                                                               <  2:1820380/140‑1 (MQ=255)

                                                                     gtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGa                                                             >  1:5545158/1‑140 (MQ=255)

                                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATATTTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGTCGAATTGATTACTTCGCAGCCTGTGGAt                                                            >  2:2234983/1‑140 (MQ=255)

                                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                            >  2:3558096/1‑140 (MQ=255)

                                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                            <  1:2351429/140‑1 (MQ=255)

                                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                            <  1:2453167/140‑1 (MQ=255)

                                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                            <  1:2864102/140‑1 (MQ=255)

                                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                            <  1:3822671/140‑1 (MQ=255)

                                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                            >  2:843046/1‑140 (MQ=255)

                                                                      tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                            <  2:2068960/140‑1 (MQ=255)

                                                                           aTGACCCTCTTGACATTTCGGGCAAATAATTCGCGAAGATAATTAGGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAttg                                                       >  2:3165207/1‑140 (MQ=255)

                                                                            tGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCAAAAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                       <  1:870153/140‑1 (MQ=255)

                                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                     <  2:1702323/140‑1 (MQ=255)

                                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                     <  1:977610/140‑1 (MQ=255)

                                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                     <  1:451292/140‑1 (MQ=255)

                                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                     <  1:4665583/140‑1 (MQ=255)

                                                                             gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                     <  2:366794/140‑1 (MQ=255)

                                                                              aCCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGGGTCg                                                    >  1:2315523/1‑140 (MQ=255)

                                                                                ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                  >  1:922246/1‑140 (MQ=255)

                                                                                   ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                               >  2:2872501/1‑140 (MQ=255)

                                                                                       acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                           <  2:117771/140‑1 (MQ=255)

                                                                                       acaTTTCGGGCAAATAAGTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGACTTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                           <  2:1333210/140‑1 (MQ=255)

                                                                                          tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAg                                        >  1:1772174/1‑140 (MQ=255)

                                                                                           ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAgg                                       >  1:5475950/1‑140 (MQ=255)

                                                                                              gggCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                    >  1:5379968/1‑140 (MQ=255)

                                                                                              gggCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                    >  2:607870/1‑140 (MQ=255)

                                                                                                    ataatTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAAc                             <  1:3287698/140‑1 (MQ=255)

                                                                                                      aatTCGCGAAGATAATTAAGACCTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCg                           <  1:5339349/140‑1 (MQ=255)

                                                                                                      aatTCGCGAAGATAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCg                           <  2:1086361/140‑1 (MQ=255)

                                                                                                             gAAGATAGTGATGAACTCAGTGCG‑‑AAACA‑AACTGTCGCAGTGGCCGGTTTCGGTTGTTACTCAAGCTTTCAGACGTATTGTGTACTTCGCAGCCTGTGGATCAGTGGCGTCTTTAGCACA‑GTCAGAATAACCGGGGTtgg                    >  2:3033419/1‑139 (MQ=255)

                                                                                                                 aTAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCCATGGCCGGTTTCCGTTGTTGCTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGAc                <  2:4847898/140‑1 (MQ=255)

                                                                                                                 aTAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGAc                <  1:3024296/140‑1 (MQ=255)

                                                                                                                 aTAATTAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTCCCGTTGCTACTCAAGCTTTCAGACGAATTGAGTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGAc                <  1:4248582/140‑1 (MQ=255)

                                                                                                                      tAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGACATGTc           <  1:1194900/140‑1 (MQ=255)

                                                                                                                      tAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGACATGTc           <  2:5442022/140‑1 (MQ=255)

                                                                                                                      tAAGAACTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGACATGTc           <  2:277873/140‑1 (MQ=255)

                                                                                                                          aaCTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGACATGTCCGGt       <  2:1412198/140‑1 (MQ=255)

                                                                                                                           aCTCAG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGCCGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGATTTGATGACATGTCCGGTa      >  2:3978901/1‑140 (MQ=255)

                                                                                                                               aG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:3356906/1‑140 (MQ=255)

                                                                                                                               aG‑GCCA‑AAAAA‑AACCGGCGCAATGGCCGGTATCCGTTGTTACTCAAGCTTTTAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCTTAATCAGCACAGGTCTG‑ATAACCGGAGTTGATGACATGTCCGGTatcat  >  1:3351899/1‑140 (MQ=255)

AAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAG‑GCC‑‑AAAAA‑AACCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTG‑ATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826545‑1826810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`