BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,855,625	+C	intergenic (+116/‑50)	sppA → / → ansA	signal peptide peptidase SppA/asparaginase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,855,621	1	.	C	95.7%	1181.4 / 41.5	371	intergenic (+112/‑54)	sppA/ansA	signal peptide peptidase SppA/asparaginase
Reads supporting (aligned to +/- strand): ref base . (4/12); new base C (125/230); total (129/242)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 390 total aligned reads displayed.

TTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑C‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTGTC  >  NZ_CP025268/1855482‑1855757

   ccTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑c‑cc                                                                                                                                        <  2:5240337/140‑1 (MQ=255)

    cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑c‑ccc                                                                                                                                       <  1:127889/140‑1 (MQ=255)

        ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑c‑cccctgc                                                                                                                                   <  2:393143/140‑5 (MQ=255)

        ccTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGAAAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑c‑cccctgc                                                                                                                                   <  1:91834/140‑5 (MQ=255)

          tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCTGCCATATTGTTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑c‑ccccggcct                                                                                                                                 <  1:3990400/140‑7 (MQ=255)

          tGCGCCAACATGCGTTAAATCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCt                                                                                                                                 <  2:3978126/140‑1 (MQ=255)

          tGCGACAAGATGCGTGATGTCTTGTATTGAGTGGCCGCCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTAGCCCCATATTTCCGAATCCGCAGAGTATCAGGTGCG‑C‑T‑c‑ccccggccg                                                                                                                                 <  2:2496098/140‑7 (MQ=255)

           gcgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTc                                                                                                                                <  2:4771624/140‑1 (MQ=255)

             gcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATCTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCAc                                                                                                                              <  2:4080750/140‑1 (MQ=255)

                  cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGtata                                                                                                                         <  1:2785486/140‑1 (MQ=255)

                           aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCt                                                                                                                                 <  2:1964834/123‑1 (MQ=255)

                           aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCt                                                                                                                                 >  1:1964619/1‑123 (MQ=255)

                           aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGc                                                                                                                >  2:1324193/1‑140 (MQ=255)

                           aGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGCGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGc                                                                                                                >  2:3804213/1‑140 (MQ=255)

                            gTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGct                                                                                                               <  1:5114762/140‑1 (MQ=255)

                               ttGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCtt                                                                                                            >  1:1455529/1‑140 (MQ=255)

                                tGTACTGAGTGGCCGACAGATCGTCGGCCCCATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTAGCCGCATAGTATCAGGTGCG‑C‑T‑CTCCC‑TGCCTCACGTATATACTTTTGCTCttt                                                                                                           >  2:1827756/1‑140 (MQ=255)

                                tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTGATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCttt                                                                                                           <  2:256498/140‑1 (MQ=255)

                                 gTACTGAGTGGCCGACAGATCGTGGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATGTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTc                                                                                                          >  2:1548623/1‑140 (MQ=255)

                                     tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGCATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                      <  2:4827727/140‑1 (MQ=255)

                                     tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCt                                                                                                                                 >  2:2185548/1‑113 (MQ=255)

                                     tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                      <  2:4319102/140‑1 (MQ=255)

                                     tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGata                                                                                                      <  1:725444/140‑1 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCGCTCTGCTTCGCCCCATATTTCCTTATCCGCATAGGATCAGGGGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:3355371/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCGATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:4984775/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:207597/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:2145019/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:444159/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:1077910/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:525713/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:3847195/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:1009180/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:5552240/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:536731/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  2:882975/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:1049688/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:3627746/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:383310/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:2145666/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:2974633/1‑140 (MQ=255)

                                       aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                    >  1:2737490/1‑140 (MQ=255)

                                        gTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtca                                                                                                   <  1:1101599/140‑1 (MQ=255)

                                         tGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcat                                                                                                  >  1:4981548/1‑140 (MQ=255)

                                          ggCCGACCGATCGTCGCCCACATTATTTCTTACGTCGGCGGATCCTCTTCCCGCTCTTTCGCCGCATATTTCCATATCCTCACAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTAACGTATATAATTTTGTTCTTTCGATAtcatc                                                                                                 <  2:4643573/140‑2 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTGTTTTTTACGTCGTCGAGTCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATGCGCATTGTGTCAGGTGTG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAgcatt                                                                                                 <  2:2796724/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:5198375/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:2928453/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:2584273/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  2:509504/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:1780996/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  1:3399178/140‑1 (MQ=255)

                                          ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                                 <  2:61841/140‑1 (MQ=255)

                                           gCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTGTATACTTTTGCTCTTTCGATAtcattc                                                                                                <  2:144050/140‑1 (MQ=255)

                                            ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                               >  2:2950789/1‑140 (MQ=255)

                                               aCAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat                                                                                            <  1:232004/140‑1 (MQ=255)

                                                    tcgtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatatcaata                                                                                       <  1:1575293/140‑1 (MQ=255)

                                                     cgtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatatcaatat                                                                                      >  2:3442827/1‑140 (MQ=255)

                                                          gCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGc                                                                                 >  1:3118711/1‑140 (MQ=255)

                                                               attattTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAg                                                                            >  2:2356195/1‑140 (MQ=255)

                                                                           gTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTAc                                                                >  1:4895153/1‑140 (MQ=255)

                                                                            tCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCCGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                                               <  1:4313559/140‑1 (MQ=255)

                                                                                               cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGAc                                            <  1:1365725/140‑1 (MQ=255)

                                                                                               cTGTTTCGCCCCATATTTCCTTACCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGAc                                            <  1:2188843/140‑1 (MQ=255)

                                                                                                    tCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCg                                       <  2:2244280/140‑1 (MQ=255)

                                                                                                           atatTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCa                                <  1:3953439/140‑1 (MQ=255)

                                                                                                            tatTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAg                               >  2:3600837/1‑140 (MQ=255)

                                                                                                            tatTTCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAg                               >  2:3703785/1‑140 (MQ=255)

                                                                                                                tCCTTATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGtt                           <  2:3230412/140‑1 (MQ=255)

                                                                                                                    tATCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGa                       >  1:323139/1‑140 (MQ=255)

                                                                                                                     ctCCGGAGAGTATGAGGGGCG‑G‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATAGCATGCAAAATACAGCAATTTACGTTGCCTACACGGGCGGGACCAGCGGGATGCAGCGTTCCGAg                      <  2:5040738/139‑1 (MQ=255)

                                                                                                                     aTCCGCGTAGTATCAGGTGCG‑CGC‑C‑CCC‑TGCCTCACGTATATACTGTTTCTCTTTCGATATCATTCATATCAATATCATTCAAAAGAAATCAATTTACGTTGCCTACACTGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  2:4905283/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGTATCCGGTGCG‑CGC‑C‑CCC‑TGCCTCACGTATATACTTTTGCTGTTTCGATATCATTCATATCAATATCATGCAAACGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  1:4553403/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGTATCAGGTGCGCC‑C‑C‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTAAACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  2:1147810/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTCTATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTAAACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  1:3894029/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  1:5310438/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      <  2:5445194/140‑1 (MQ=255)

                                                                                                                     aTCCGCATAGCGTCAGGTGCG‑C‑TCC‑CCC‑GGCCTAACGTATATACGTTTGCTCTTTCGATATCAGTCATATCAATATCATGGGACGGCAATCAACTGCCGTTGCCTACACGGGCGGGACCATCGGGATGACGCGTTCCGAg                      <  2:3094385/140‑1 (MQ=255)

                                                                                                                      tCCGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc                     >  1:588646/1‑140 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGGTATCGTTCATATCAGTCTCCTGGAAAAGCCATCAATTTACGTTGCCTACACGGGCGAGACCCACGGCATGCAGCGTCCCGCGCa                    <  1:4390146/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAACTCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:3118711/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  1:1657326/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  1:3021549/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:305097/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  1:4799809/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  1:829587/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  2:1015627/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  1:2439578/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  1:3308923/140‑1 (MQ=255)

                                                                                                                       ccGCATAGTATCAGGTGCG‑C‑T‑C‑CCCACGCCTCACGTATATCCTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACTTTGCCTACACGGGCGGGACCACCGGGATGCAGCGTTCCGAGCa                    <  2:12225/140‑1 (MQ=255)

                                                                                                                       cagCATAGTATCGGCGGTG‑C‑T‑C‑CCCTTGCCGCACGTGTATATTTTTGCGGGTTGTATCTCATTCGTAGCAATCTCAGCGAATAGAAATCGATTCTCGTTGACTCGACGGGCGGGACCGTCGGGATGCAGCGTTCCGAGCa                    <  2:2399771/138‑1 (MQ=255)

                                                                                                                        cGCATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAg                   >  1:4440428/1‑140 (MQ=255)

                                                                                                                          cATAGTATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAggg                 <  1:4574862/140‑1 (MQ=255)

                                                                                                                               tATCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcggg                                              >  2:1619383/1‑106 (MQ=255)

                                                                                                                                aTCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg                      >  2:4554696/1‑129 (MQ=255)

                                                                                                                                 tCAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtatata          >  1:5246518/1‑140 (MQ=255)

                                                                                                                                  cAGGTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAGTGACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc         <  2:5603463/140‑1 (MQ=255)

                                                                                                                                    ggTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtatata          >  1:3070474/1‑137 (MQ=255)

                                                                                                                                    ggTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCg       >  1:3146931/1‑140 (MQ=255)

                                                                                                                                    ggTGCG‑C‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCg       >  1:5332524/1‑140 (MQ=255)

                                                                                                                                       gcg‑c‑TCC‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa                    <  1:1844519/124‑1 (MQ=255)

TTGCCTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCG‑C‑T‑C‑CCC‑TGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTGTC  >  NZ_CP025268/1855482‑1855757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`