BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,879,869	(C)_5→6	pseudogene (521/821 nt)	nimR ←	DNA‑binding transcriptional regulator NimR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,879,864	1	.	C	96.8%	810.2 / 17.3	253	pseudogene (526/821 nt)	nimR	DNA‑binding transcriptional regulator NimR
Reads supporting (aligned to +/- strand): ref base . (5/3); new base C (120/122); total (128/125)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 291 total aligned reads displayed.

CCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑C‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGG  >  NZ_CP025268/1879726‑1879998

ccTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c                                                                                                                                         >  1:625105/1‑140 (MQ=255)

ccTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c                                                                                                                                         >  2:497749/1‑140 (MQ=255)

 cTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c‑c                                                                                                                                       <  2:1089097/140‑1 (MQ=255)

   ttCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c‑ccc                                                                                                                                     <  2:3573138/140‑1 (MQ=255)

   ttCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c‑ccc                                                                                                                                     >  2:4153503/1‑140 (MQ=255)

     cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCATTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c‑ccccc                                                                                                                                   >  2:2155244/1‑139 (MQ=255)

     cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c‑ccccc                                                                                                                                   >  2:1640719/1‑139 (MQ=255)

     cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c‑ccccc                                                                                                                                   >  1:3419299/1‑139 (MQ=255)

     cACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑c‑c                                                                                                                                       >  1:1837989/1‑136 (MQ=255)

           tCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGa                                                                                                                             >  2:4956107/1‑140 (MQ=255)

               tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAgg                                                                                                                         >  1:5279965/1‑140 (MQ=255)

               tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAgg                                                                                                                         >  1:1128222/1‑140 (MQ=255)

               tGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAgg                                                                                                                         >  1:5545129/1‑140 (MQ=255)

                gAAGCGCCATAATCAGCTGTAACTGCTGACGCGACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑CGCCCC‑ACTCGACAGGc                                                                                                                        >  2:1539532/1‑140 (MQ=255)

                gAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGc                                                                                                                        <  2:3480121/140‑1 (MQ=255)

                gAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGc                                                                                                                        >  1:4221379/1‑140 (MQ=255)

                  aGCGCCATAATAAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCATAAAAGCCAGCCCATTGCCCAAAT‑G‑C‑CCCCGACTCGACAGGccc                                                                                                                      >  2:3220468/1‑140 (MQ=255)

                    cgcCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCtt                                                                                                                    >  1:2608132/1‑140 (MQ=255)

                      ccATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTc                                                                                                                  >  2:515384/1‑140 (MQ=255)

                       cATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAGAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCg                                                                                                                 <  1:5381715/140‑1 (MQ=255)

                         tAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGcc                                                                                                               <  1:4485340/140‑1 (MQ=255)

                         tAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGcc                                                                                                               <  1:734597/140‑1 (MQ=255)

                            tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCcatc                                                                                                            <  2:5061796/140‑1 (MQ=255)

                            tCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCcatc                                                                                                            <  2:3101591/140‑1 (MQ=255)

                            tCAGCTGTAACTGCTGACGCCACTCGCGAAATCTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑CCTCGACAGGCCCTTTCGCcatc                                                                                                            <  1:1236641/140‑1 (MQ=255)

                            tCAGCTGTAACGGCTGACGCCACTGGCGAAAACTCAACCCCGGCTCTGTTACTATCAGTCGGGCAAGATGGCGTTCACTCATCGCAAAAAAGCCAGCCCATGGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCcatc                                                                                                            <  2:2565843/140‑1 (MQ=255)

                            tCAGATGTAACTGCTGGCGCCACAGGCAAACGCCCAACCCCGTCTATATTACCATCAGTCGGGCAAGATTGCGTTAACTCCTCGAAAAACCGCCAGCCCATCGCCCCACT‑GCC‑CCCC‑CCTCGCCAGGCCCTTTCGCcatc                                                                                                            <  2:1592275/140‑1 (MQ=255)

                              aGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCcatcat                                                                                                          <  1:715087/140‑1 (MQ=255)

                                   tAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTcca                                                                                                     <  1:3022010/140‑1 (MQ=255)

                                   tAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTcca                                                                                                     >  2:4180583/1‑140 (MQ=255)

                                         ctgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCg                                                                                               >  2:3744154/1‑140 (MQ=255)

                                          tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGt                                                                                              >  2:86211/1‑140 (MQ=255)

                                          tgACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGt                                                                                              >  1:2314545/1‑140 (MQ=255)

                                           gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑CCTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                             <  1:1015522/140‑1 (MQ=255)

                                           gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                             <  1:1349500/140‑1 (MQ=255)

                                           gACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTg                                                                                             <  1:3217379/140‑1 (MQ=255)

                                            aCGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGc                                                                                            >  2:186316/1‑140 (MQ=255)

                                             cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCg                                                                                           >  2:5384639/1‑140 (MQ=255)

                                             cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCg                                                                                           <  1:4153503/140‑1 (MQ=255)

                                             cGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCg                                                                                           >  1:1864551/1‑140 (MQ=255)

                                                 aCTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAtt                                                                                       >  1:5332646/1‑140 (MQ=255)

                                                 aCTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAtt                                                                                       >  2:3845276/1‑140 (MQ=255)

                                                   tGGCGAAAACTCAACCCCGTCGCGTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATTGCAAAAAAGCCAGCCCATTGCCCTAATGG‑C‑CCCC‑ACCCGAAAGGCCCTTTCGCCATCATGTCCACCATCGTGCGGAtctt                                                                                     >  2:1188099/1‑140 (MQ=255)

                                                     gCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAg                                                                                   >  2:1825199/1‑140 (MQ=255)

                                                           accccaCCCCGTCCCTTTTCCTCTCAGTCGGGCAAGATTGCGTTCGCTCATCCCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATgag                                                                             <  2:1303135/135‑1 (MQ=255)

                                                            cTCAACCCCGTCTCTTTTACTATCAGTCGCGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGAGTTTAGGATgaga                                                                            <  1:560382/140‑1 (MQ=255)

                                                               aaCCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGagaaga                                                                         >  1:5081874/1‑140 (MQ=255)

                                                                 ccccGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGaaa                                                                       <  2:2431592/140‑1 (MQ=255)

                                                                 ccccGTCTCTTTAACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGaaa                                                                       <  2:3937863/140‑1 (MQ=255)

                                                                    cGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACgg                                                                    <  1:4715320/140‑1 (MQ=255)

                                                                     gTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACggg                                                                   <  2:1878980/140‑1 (MQ=255)

                                                                     gTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACggg                                                                   <  1:4028825/140‑1 (MQ=255)

                                                                     gTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACggg                                                                   <  2:2854980/140‑1 (MQ=255)

                                                                      tctcTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGc                                                                  <  2:1397411/140‑1 (MQ=255)

                                                                           tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGta                                                             >  1:4943769/1‑139 (MQ=255)

                                                                           tttACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTg                                                             >  1:4536744/1‑140 (MQ=255)

                                                                           tttACTATCAGTCAGGCAAGATGGCGTTCACTCATCGCAAAAAAGGCAGCCGATTGCCGAAAT‑GAC‑CTCC‑ACTCGACAGACCCGTTCGCCATCATCTCCACGATAGTGCGGATCTTAGGATGAGAAGAAAAGGTCAGGtt                                                             >  2:313510/1‑139 (MQ=255)

                                                                                  tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgt                                                      >  2:1122439/1‑140 (MQ=255)

                                                                                  tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgt                                                      >  1:5478513/1‑140 (MQ=255)

                                                                                  tCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgt                                                      >  1:51085/1‑140 (MQ=255)

                                                                                    aGTCGGGCATGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                    >  1:4601672/1‑140 (MQ=255)

                                                                                    aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                    >  1:3640008/1‑140 (MQ=255)

                                                                                    aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                    >  1:4305929/1‑140 (MQ=255)

                                                                                    aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                    >  1:869344/1‑140 (MQ=255)

                                                                                    aGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                    >  1:1966608/1‑140 (MQ=255)

                                                                                    aGTCGGGCAAGATTGCGTCCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttg                                                    >  2:4891134/1‑140 (MQ=255)

                                                                                     gTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgt                                                   <  2:4877521/140‑1 (MQ=255)

                                                                                      tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                                  >  2:1032806/1‑140 (MQ=255)

                                                                                      tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                                  >  1:2173380/1‑140 (MQ=255)

                                                                                      tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCtgttgtt                                                  >  2:2119546/1‑140 (MQ=255)

                                                                                      tCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCt                                                        >  2:4694744/1‑134 (MQ=255)

                                                                                         ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGCGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                               <  2:4349559/140‑1 (MQ=255)

                                                                                         ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                               <  1:4843614/140‑1 (MQ=255)

                                                                                         ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                               >  2:2647514/1‑140 (MQ=255)

                                                                                         ggCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTtgtg                                               <  2:3735780/140‑1 (MQ=255)

                                                                                          gCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCa                                                                 >  2:5584586/1‑121 (MQ=255)

                                                                                           cAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgc                                             <  2:1775390/140‑1 (MQ=255)

                                                                                           cAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgc                                             <  1:3602912/140‑1 (MQ=255)

                                                                                           cAAGATGGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGgc                                             <  2:3621810/140‑1 (MQ=255)

                                                                                                ttGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGctgctg                                        >  1:107212/1‑140 (MQ=255)

                                                                                                    gTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAggg                                    >  2:3819583/1‑140 (MQ=255)

                                                                                                    gTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATGTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAggg                                    >  1:2931415/1‑140 (MQ=255)

                                                                                                     ttCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                                   <  2:443357/140‑1 (MQ=255)

                                                                                                     ttCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                                   <  2:4561538/140‑1 (MQ=255)

                                                                                                     ttCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGa                                   <  2:5503847/140‑1 (MQ=255)

                                                                                                      tCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTGTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAg                                  <  1:5150292/140‑1 (MQ=255)

                                                                                                        aCTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGGGtt                                >  1:2377050/1‑140 (MQ=255)

                                                                                                          tCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtca                              <  2:4075642/140‑1 (MQ=255)

                                                                                                          ccaccgaaaaaacGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCAATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtca                              <  2:4525199/133‑1 (MQ=255)

                                                                                                             tCGCAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTtcatca                           >  2:7669/1‑140 (MQ=255)

                                                                                                                cAAAAAAGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAAc                        <  2:3373109/140‑1 (MQ=255)

                                                                                                                  aaaaaGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGCTCATCAAACAg                      >  2:3275689/1‑140 (MQ=255)

                                                                                                                     aaGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc                   <  1:252754/140‑1 (MQ=255)

                                                                                                                     aaGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc                   <  2:5469876/140‑1 (MQ=255)

                                                                                                                     aaGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGAc                   <  1:2259682/140‑1 (MQ=255)

                                                                                                                     aaGCCAGCCCATTGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCGCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCCAACAGGAc                   <  1:1709075/140‑1 (MQ=255)

                                                                                                                                 tGCCCCAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCt       >  1:159209/1‑140 (MQ=255)

                                                                                                                                   ccccAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGc     <  1:952877/140‑1 (MQ=255)

                                                                                                                                     ccAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCgg   <  1:517488/140‑1 (MQ=255)

                                                                                                                                      cAAT‑GCC‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCggg  >  1:4451626/1‑140 (MQ=255)

CCTTTCACTAATCCCTGAAGCGCCATAATCAGCTGTAACTGCTGACGCCACTGGCGAAAACTCAACCCCGTCTCTTTTACTATCAGTCGGGCAAGATTGCGTTCACTCATCGCAAAAAAGCCAGCCCATTGCCCCAAT‑G‑C‑CCCC‑ACTCGACAGGCCCTTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGG  >  NZ_CP025268/1879726‑1879998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`