BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,913,492	+T	coding (88/144 nt)	mgrB ←	PhoP/PhoQ regulator MgrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,913,492	1	.	T	99.7%	1213.9 / ‑3.8	321	coding (88/144 nt)	mgrB	PhoP/PhoQ regulator MgrB
Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (196/124); total (197/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 336 total aligned reads displayed.

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

ttCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTg                                                                                                                                        >  1:4889686/1‑140 (MQ=255)

  cGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTgta                                                                                                                                      >  1:2040698/1‑138 (MQ=255)

         ttCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGcaca                                                                                                                               <  2:2578876/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  2:2986130/140‑1 (MQ=255)

           caccacCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatc                                                                                                                             <  2:3044806/140‑1 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            <  1:285571/140‑1 (MQ=255)

            accaccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCAcatca                                                                                                                            >  1:2641714/1‑140 (MQ=255)

              caccTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATg                                                                                                                          <  2:887367/140‑1 (MQ=255)

                  tctGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGa                                                                                                                      >  1:3941620/1‑140 (MQ=255)

                      aGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATAc                                                                                                                  >  2:2094125/1‑140 (MQ=255)

                        tAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCt                                                                                                                <  1:2352648/140‑1 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:1973985/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:241480/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:3061182/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:26612/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:1555131/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  1:3261033/1‑140 (MQ=255)

                           agGCGCTATTCTACCACTGCGGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTgcg                                                                                                             >  2:2805701/1‑140 (MQ=255)

                               gcTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCa                                                                                                         <  2:4884041/140‑1 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:5322268/1‑140 (MQ=255)

                                cTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCaa                                                                                                        >  1:4963083/1‑140 (MQ=255)

                                cTATTCTACCACGGCTGGAGAGGAAGAAAGTCTAGTGCTG‑AAAAAATTATATCACGACGGGATAAACGGGTTAATGGCACAAATTCCGCTGAAATATTGTACATCCTGTATCGCACATCATGGGGAATACCTGCGCCCaa                                                                                                        >  2:4437607/1‑140 (MQ=255)

                                  aTTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTCCGCCCAAag                                                                                                      >  1:123147/1‑140 (MQ=255)

                                    tCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAagca                                                                                                    <  1:5471382/140‑1 (MQ=255)

                                      tACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAagcagc                                                                                                  <  2:241480/140‑1 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  1:5093568/140‑1 (MQ=255)

                                         cACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  1:3164780/140‑1 (MQ=255)

                                         aaCTGCTGGAGAGGCAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAgcagcagg                                                                                               <  2:1972348/139‑3 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  2:1657572/1‑140 (MQ=255)

                                           ctgctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaagca                                                                                             >  1:4343585/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTGAAAAAAATGATATCACCACGGGATAAACTGGGTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCACCCAAAGCAacaagcaa                                                                                            >  2:689154/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTCATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:4633244/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:3683802/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:1531592/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:1293904/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:3837817/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  2:4694471/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:380765/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:927722/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaagcaag                                                                                           >  1:3621754/1‑140 (MQ=255)

                                             gctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGcaaacaag                                                                                           >  2:4235939/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAACTGATATCACCACGGGCTAAACGGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTAGCGCACATCATCTTGAATACCCGCGCCCAAAGCAGCaggcaagc                                                                                          >  2:3345377/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  1:4064145/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  2:2074315/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  1:963538/1‑140 (MQ=255)

                                              ctgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCaagcaagc                                                                                          >  1:3478354/1‑140 (MQ=255)

                                               tgGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGcc                                                                                         <  2:4625798/140‑1 (MQ=255)

                                                 gagaGCAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCaa                                                                                       <  2:4922940/140‑1 (MQ=255)

                                                      gaagcaAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATAGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCAAGTTGAATACCTGCGCCCAAAGCAGTAAGCAAGCCAAaacca                                                                                  >  2:1307739/1‑140 (MQ=255)

                                                        agaaAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               >  2:2437375/1‑125 (MQ=255)

                                                        agaaAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACg                                                                                >  1:1917547/1‑140 (MQ=255)

                                                                 aGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGa                                                                       >  2:4168474/1‑140 (MQ=255)

                                                                   tGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAAc                                                                     >  2:1013913/1‑140 (MQ=255)

                                                                    gCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACg                                                                    >  1:3750942/1‑140 (MQ=255)

                                                                      tG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGAc                                                                  <  1:1003233/140‑1 (MQ=255)

                                                                         aaaaaaTGATATCACCACGAGATAGACTGGTTAATGGCAAAAGTTCCGCTGAAAAATTGTACATCCTGTGTCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACACCCAGAACGAccc                                                                >  2:269598/1‑140 (MQ=255)

                                                                                          cGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACt                                               <  2:4061136/140‑1 (MQ=255)

                                                                                          cGGGATAAACTGGTTAATGGCACAAATTCCGCGGAAAAATTGTACATCCTGTATCGCACAGCATGTTAAATACCTGCGCCCAAAGCAGCAAGCAAGCTAACACCACGACAAACAGAACGACCCATGGAAAGTTTTTCACt                                               >  2:2649475/1‑140 (MQ=255)

                                                                                            ggATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTCTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTcc                                             >  1:658055/1‑140 (MQ=255)

                                                                                                aaaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtc                                         <  1:2049313/140‑1 (MQ=255)

                                                                                                 aaCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtct                                        <  2:3235243/140‑1 (MQ=255)

                                                                                                  aCTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAAtctc                                       >  1:61872/1‑140 (MQ=255)

                                                                                                     ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  2:5240141/140‑1 (MQ=255)

                                                                                                     ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  1:1163141/140‑1 (MQ=255)

                                                                                                     ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  1:170662/140‑1 (MQ=255)

                                                                                                     ggTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGt                                    <  1:4178708/140‑1 (MQ=255)

                                                                                                      gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   >  1:2494878/1‑140 (MQ=255)

                                                                                                      gTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGtt                                   >  1:5503930/1‑140 (MQ=255)

                                                                                                       ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCagcaag                                                                                               <  1:4750340/79‑1 (MQ=255)

                                                                                                       ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  2:1933071/1‑140 (MQ=255)

                                                                                                       ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:1432557/1‑140 (MQ=255)

                                                                                                       ttAATGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGttt                                  >  1:3892152/1‑140 (MQ=255)

                                                                                                          aTGGCAGAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:5418829/140‑1 (MQ=255)

                                                                                                          aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  1:3046346/140‑1 (MQ=255)

                                                                                                          aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:4852902/140‑1 (MQ=255)

                                                                                                          aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:4044501/140‑1 (MQ=255)

                                                                                                          aTGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCac                               <  2:1132812/140‑1 (MQ=255)

                                                                                                           tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCACCACCACGACAACCAGACCGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  1:4984617/1‑140 (MQ=255)

                                                                                                           tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              <  1:4976068/140‑1 (MQ=255)

                                                                                                           tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              >  2:5299268/1‑140 (MQ=255)

                                                                                                           tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              <  1:4165271/140‑1 (MQ=255)

                                                                                                           tGGCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacc                              <  2:4755026/140‑1 (MQ=255)

                                                                                                            ggCACAAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTGTCacct                             >  2:586711/1‑140 (MQ=255)

                                                                                                                caAATTCCGCTGAAATTTTGTACATCCTGTATCGCCCATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACTACCAGAACGACCCATCGAAACTTATTCACTCCAATCTCCGTTTCtcctactt                         >  2:143626/1‑138 (MQ=255)

                                                                                                                caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCg                                     <  2:2234259/128‑1 (MQ=255)

                                                                                                                caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCg                                     >  1:2234259/1‑128 (MQ=255)

                                                                                                                caAATTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCacctacct                         >  2:3642586/1‑140 (MQ=255)

                                                                                                                   aTTCCGCTGAACAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  2:5133380/140‑1 (MQ=255)

                                                                                                                   aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  2:1259284/140‑1 (MQ=255)

                                                                                                                   aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  1:2550959/140‑1 (MQ=255)

                                                                                                                   aTTCCGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTAt                      <  1:1747258/140‑1 (MQ=255)

                                                                                                                      ccGCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTc                   >  2:1853729/1‑140 (MQ=255)

                                                                                                                        gCTGAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCAt                 >  1:4902036/1‑140 (MQ=255)

                                                                                                                           gAAAAATTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTaa              <  2:26612/140‑1 (MQ=255)

                                                                                                                            aaaaaTTGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTaaa             <  2:31738/140‑1 (MQ=255)

                                                                                                                                 ttGTACATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGc        <  2:946269/140‑1 (MQ=255)

                                                                                                                                     aCATCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGc    <  1:4124392/140‑1 (MQ=255)

                                                                                                                                       aTCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCACGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  1:3005593/140‑1 (MQ=255)

                                                                                                                                       aTCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  1:2932857/140‑1 (MQ=255)

                                                                                                                                       aTCCTGTATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCgt  <  1:3723476/140‑1 (MQ=255)

TTCGTTCATTTCACCACCTCTGAGTAGAGGCGCTATTCTACCACTGCTGGAGAGGAAGAAAATCTAGTGCTG‑AAAAAATGATATCACCACGGGATAAACTGGTTAATGGCACAAATTCCGCTGAAAAATTGTACATCCTG‑ATCGCACATCATGTTGAATACCTGCGCCCAAAGCAGCAAGCAAGCCAACACCACGACAACCAGAACGACCCATCGAAACTTTTTCACTCCAATCTCCGTTTCACCTACCTTATGTCATTAAACTAGCATGCGT  >  NZ_CP025268/1913353‑1913625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`