BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,292,596	(C)_6→7	pseudogene (361/2531 nt)	yejO ←	autotransporter YejO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,292,590	1	.	C	96.3%	863.9 / 23.1	270	pseudogene (367/2531 nt)	yejO	autotransporter YejO
Reads supporting (aligned to +/- strand): ref base . (5/5); new base C (107/153); total (112/158)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑C‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATGCCCCCTCTTTAACCTCCAGAAGACTTCCCTTTTCGATAGCGACAAAGACgg                                                                                                                                          >  1:5113573/1‑140 (MQ=255)

aCCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGCCgg                                                                                                                                          >  1:4482433/1‑140 (MQ=255)

 ccGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑c                                                                                                                                        <  2:3362002/140‑1 (MQ=255)

     gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑c‑ccc‑c                                                                                                                                  >  1:4288298/1‑140 (MQ=255)

     gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCATTTCGATAGCGACAAAGACGG‑c‑ccc‑c                                                                                                                                  >  2:4398521/1‑140 (MQ=255)

          tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGGTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGATGG‑c‑ccc‑cccatc                                                                                                                             >  2:3950497/1‑136 (MQ=255)

            tGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCtt                                                                                                                            >  1:685796/1‑140 (MQ=255)

               tCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTAATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCtttgt                                                                                                                         >  2:3341837/1‑138 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAAc                                                                                                                     >  2:2424760/1‑140 (MQ=255)

                   aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAAc                                                                                                                     >  1:2913751/1‑140 (MQ=255)

                       ccTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGAtt                                                                                                                 <  2:185643/140‑1 (MQ=255)

                        cTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTa                                                                                                                >  2:4084936/1‑140 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGcgccc                                                                                                         <  2:843692/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGcgccc                                                                                                         <  1:3191821/140‑1 (MQ=255)

                               gCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGcgccc                                                                                                         <  1:2743335/140‑1 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  1:1622573/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  1:1949223/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  2:2973123/1‑140 (MQ=255)

                                  cgcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGcgcccgcc                                                                                                      >  1:1425801/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCtt                                                                                                    >  2:320683/1‑140 (MQ=255)

                                    cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATAAAGCGCCCGCCtt                                                                                                    >  2:4859734/1‑140 (MQ=255)

                                     gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATTCAAATCCCCCCTCTTTAACCTCCAGGAGTTCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCTCCCGCCttt                                                                                                   >  2:2062481/1‑140 (MQ=255)

                                     gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCttt                                                                                                   <  1:1571861/140‑1 (MQ=255)

                                         ttgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATc                                                                                               >  1:4820705/1‑140 (MQ=255)

                                          tgttTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg                                                                                              <  2:4713861/140‑1 (MQ=255)

                                            ttttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTg                                                                                            <  1:3279690/140‑1 (MQ=255)

                                              ttAATCGCACCGCCCGCTCCTTGATATACCCCAAATGCAAATCCCCCCTCCCTAACCCCCAGTAGTCCTCCCTTTTCGATAGCGACCAAGACGG‑C‑CCCTCCATCTTTTTCAACGATTAAGCGCCCGCCTCTTTTCGTGGt                                                                                          >  2:5025223/1‑140 (MQ=255)

                                              ttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGt                                                                                          >  1:1483325/1‑140 (MQ=255)

                                              ttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGt                                                                                          <  2:1430950/140‑1 (MQ=255)

                                                 aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATc                                                                                       <  2:114738/140‑1 (MQ=255)

                                                 aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATc                                                                                       <  1:1187585/140‑1 (MQ=255)

                                                 aTAGCACCGCCTGCGTTCTGATCTACCGCAAATGCAAAGCCCCCCTCTTTAACCTCCCTTACTCCTCCCTTTTCGACAGCGACGAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTACGCGCCCGCCTTTAAGCGTGGTATc                                                                                       <  2:2857259/140‑1 (MQ=255)

                                                   aGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGTTATCAt                                                                                     >  2:1606203/1‑140 (MQ=255)

                                                      atcGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCATCGATTAAGCGCCCGCCTTTGATCGTGGTATCATTCg                                                                                  >  1:3127888/3‑140 (MQ=255)

                                                         gCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg                                                                               <  2:685796/140‑1 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCATTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGACTTTAATCGTGGTATCATTCGCTgc                                                                              >  2:5561691/1‑140 (MQ=255)

                                                          ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTgc                                                                              >  1:745211/1‑140 (MQ=255)

                                                             gCTTTCTGATCTACCGCAAATGCAAATCCCCTCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGcc                                                                           >  2:4262201/1‑140 (MQ=255)

                                                              cTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa                                                                          <  1:1001669/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  2:1824/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa                                                                       <  2:5363902/140‑1 (MQ=255)

                                                                 tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCACCATaa                                                                       <  2:3704889/140‑1 (MQ=255)

                                                                   tGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc                                                                     <  1:2475014/140‑1 (MQ=255)

                                                                        tACCGCACATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTGTTCGATAGCGACAACGACGG‑C‑CCCACCATCTTTTTCAACGATTAAGCGACCGCCTTTAATCGTGGTATCATTAGCTTCGCCATAAACCTGTa                                                                >  2:4180604/1‑140 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  2:457177/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:5345277/140‑1 (MQ=255)

                                                                         aCCGCAAATGCAAACCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTAGTACCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:4724149/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:2947955/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:3474018/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:1111765/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:2551783/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:5445721/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  1:2332899/140‑1 (MQ=255)

                                                                           cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             <  2:754400/140‑1 (MQ=255)

                                                                             caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCGTTCGCTCCGCCATAAACCTGTAGcaca                                                           >  1:5531191/1‑140 (MQ=255)

                                                                               aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAACGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACAcc                                                         >  1:2423850/1‑140 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  1:4110815/140‑1 (MQ=255)

                                                                                atgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa                                                        <  1:509266/140‑1 (MQ=255)

                                                                                   caaatACCCCCTCTTTAACCTCCCGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑CACCC‑ACATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCatta                                                     <  1:2062481/140‑1 (MQ=255)

                                                                                      atCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTg                                                  >  1:3454128/1‑140 (MQ=255)

                                                                                            ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCAAACCATTATTGAGCGTa                                            >  1:4559120/1‑140 (MQ=255)

                                                                                                        ccAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc                                >  2:3718337/1‑140 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:2638003/1‑140 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:1712397/1‑140 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:585587/1‑140 (MQ=255)

                                                                                                         cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg                               >  1:1233668/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGCTTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  1:1021585/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:5074259/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:4840477/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:1253409/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  1:977344/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  1:932105/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:384660/1‑140 (MQ=255)

                                                                                                          agtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCACACGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTCGCACACCATTATTGAGCGTAGTCTGGTTTGCcgc                              >  2:2658493/1‑140 (MQ=255)

                                                                                                           gtagtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgcg                             <  1:2258058/140‑1 (MQ=255)

                                                                                                              gtCCTCCCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa                          <  1:4910415/140‑1 (MQ=255)

                                                                                                               tcctccCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAc                         >  2:4625643/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  2:5496976/1‑140 (MQ=255)

                                                                                                                 ctccCTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAccc                       >  1:65074/1‑140 (MQ=255)

                                                                                                                  tccCTTGTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTAAACGATTAAGCGACCGACTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCa                      <  1:2209671/140‑1 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca                                                             >  2:2297355/1‑100 (MQ=255)

                                                                                                                   cccTTTTCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt                     <  2:506930/140‑1 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg                                                               <  1:4378056/95‑1 (MQ=255)

                                                                                                                      ttttCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg                  <  2:1337911/140‑1 (MQ=255)

                                                                                                                      tttaCGATAGCGACAAATGCGG‑C‑CCCGCCCTCTTTTTCAAGGAGTAAGCTACCGCCTTTAATCGTGGTATCACTCGCTGCGCCCTAACCCTCCAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAtgtg                  <  2:4488516/140‑1 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGCAc               <  2:2277516/140‑1 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc               <  2:3151190/140‑1 (MQ=255)

                                                                                                                         tCGATAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc               <  1:3532313/140‑1 (MQ=255)

                                                                                                                            aTAGCGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAAt            >  1:2450153/1‑140 (MQ=255)

                                                                                                                                cGACAAAGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGa        >  1:395867/1‑140 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACTCATTTGAACAATTTGATAACCg  <  1:2833892/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:1358790/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:3008626/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  2:4987723/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:3042660/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:3668963/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:3691150/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:4196008/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:4625643/140‑1 (MQ=255)

                                                                                                                                      aGACGGCC‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg  <  1:5011236/140‑1 (MQ=255)

ACCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGG‑C‑CCC‑CCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG  >  NZ_CP025268/2292451‑2292723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`