BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,466,923	(C)_8→9	intergenic (+242/‑123)	fadL → / → CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,466,915	1	.	C	93.9%	450.5 / 21.5	149	intergenic (+234/‑131)	fadL/CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (1/8); new base C (11/128); total (12/137)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCA  >  NZ_CP025268/2466778‑2467054

ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑c                                                                                                                                            <  2:4045090/140‑1 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cc                                                                                                                                           >  2:2995738/1‑140 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cc                                                                                                                                           <  2:3241493/140‑1 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cc                                                                                                                                           >  2:2995737/1‑140 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cc                                                                                                                                           <  2:346421/140‑1 (MQ=255)

 tGCAAACAGCCGGTTGGCAGCTTGCGCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cc                                                                                                                                           <  2:550269/140‑1 (MQ=255)

     aaCAGCCTGTTTGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTCCCAGGATAC‑T‑‑c‑cccccc                                                                                                                                       <  1:3420121/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCCATAAGCTGTATCCACAGACAAACCTGAAGGGACAGGCCTATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑c‑‑c‑cccccc                                                                                                                                       <  2:1711027/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccc                                                                                                                                       <  1:4758237/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccc                                                                                                                                       <  1:1173592/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccc                                                                                                                                       <  2:277856/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccc                                                                                                                                       <  1:1317496/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccc                                                                                                                                       <  1:5246364/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccc                                                                                                                                       <  1:5246807/140‑1 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccccc                                                                                                                                     <  2:2573437/140‑2 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccccc                                                                                                                                     <  2:2088861/140‑2 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccccc                                                                                                                                     <  1:351521/140‑2 (MQ=255)

          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑c‑cccccccctgg                                                                                                                                  <  2:2406911/140‑5 (MQ=255)

               tGGCAGCTTGCCCTTTCAAATCAAGAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTAtt                                                                                                                             <  2:843953/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCCCAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATtgtg                                                                                                                          <  2:4005774/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATtgtg                                                                                                                          <  2:4516134/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATtgtg                                                                                                                          <  1:4166879/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATtgtg                                                                                                                          <  1:5554170/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATtgtg                                                                                                                          <  1:2230422/140‑1 (MQ=255)

                  cAGCTCGCCCTTTCAAATCAATAAGCGGTATCCACACACCAACCTGAACGCAAAGGCATATTTTCAGGCGTTCTCCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATtgtg                                                                                                                          <  2:3501061/140‑1 (MQ=255)

                    gcgtGCCCTTTCAGATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCGGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTgcg                                                                                                                        <  2:5352451/137‑1 (MQ=255)

                    gCTTGCCTTTTCAAATCAATAACCGTTACCAACAGACAAACCTGAAGGGAAAGCCATTTTTTCAGGGGTTCTCCTCGTCCTCCTCAAAGAGTTTCCTTTTCTGCATTTCCAGGTTAC‑TC‑C‑CCCCCCC‑TGGCTATTGTgcg                                                                                                                        <  1:5222130/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTgcg                                                                                                                        <  1:405628/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTgcg                                                                                                                        <  2:1275046/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTgcg                                                                                                                        <  1:4228462/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTgcg                                                                                                                        <  2:597625/140‑1 (MQ=255)

                    gCTTCCCCTTTCAAAACAATAAGAGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCCCCTCCTTCTCAAAGCGTTTACTTTGCTGCAGTGCCATGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTgcg                                                                                                                        <  2:2224935/140‑1 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TCCC‑CCCCCCC‑TGGCTATTGTGCGCTc                                                                                                                     >  1:695335/1‑140 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCa                                                                                                                    <  1:1235202/140‑1 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCa                                                                                                                    <  1:4349283/140‑1 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCa                                                                                                                    >  1:2404914/1‑140 (MQ=255)

                          ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATa                                                                                                                  <  1:162656/140‑1 (MQ=255)

                          ccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATa                                                                                                                  <  1:3223887/140‑1 (MQ=255)

                          ccTTTCAAATCAATAAGCGGTATCCACACACAACCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATa                                                                                                                  <  2:1728020/140‑1 (MQ=255)

                          catttAAACTCAATCAGCGGTATCCACACACAAACATGACGGGAAAGGCATCTTTTCAAGCGTTCTGCTCGTCCTTCTCAACGCGTTTACTTTTCTGCATTTCCCGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATa                                                                                                                  <  1:4670018/138‑1 (MQ=255)

                              tCAAATCAATAAGCGGTATCCCCAGACAACCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTCCTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACt                                                                                                              <  2:3689740/140‑1 (MQ=255)

                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACt                                                                                                              <  1:806485/140‑1 (MQ=255)

                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACt                                                                                                              <  2:4799657/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                             <  2:1830606/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                             <  2:1155672/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                             <  2:5590793/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                             <  1:5564729/140‑1 (MQ=255)

                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTc                                                                                                             <  2:4590246/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  2:2358035/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  1:3724723/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  2:1933821/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  1:4693966/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                  <  1:4852872/140‑1 (MQ=255)

                                             ctacccACACCCAACCCTCAAGGCAAAGGCATATTTCCAGACGTTCTGATGGTCATACTCAACGAGTTTACTTTTCTGCATTCCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                               <  2:787681/136‑1 (MQ=255)

                                               aTCCACAGTCAAACCTGAAGGGAAACGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTTCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             <  2:975143/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             <  1:3584813/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             <  1:3015712/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             <  1:5060029/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             <  1:4321646/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                             <  1:2378380/140‑1 (MQ=255)

                                                    caGACAAACCTGAAGGTCCAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCCCACCCAGTTTACTTTTCTGCATTTCCCGGATCCGC‑‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaca                                                                                        <  2:4907039/140‑3 (MQ=255)

                                                          aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATGTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                                  <  2:4874623/140‑1 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                                <  2:4334509/140‑1 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                                <  2:2360446/140‑1 (MQ=255)

                                                               gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TCCC‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                                                              <  2:695335/140‑1 (MQ=255)

                                                                     aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                       <  1:2868029/140‑1 (MQ=255)

                                                                     aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCCCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                       >  2:5288116/1‑140 (MQ=255)

                                                                      aaGGCATATTTTCCGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGCTAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      <  2:517977/140‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      >  2:5024385/1‑140 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      <  1:4211297/140‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      <  1:952245/140‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                      <  1:1806965/140‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTGTTTTGa                                                                      <  1:1851281/140‑1 (MQ=255)

                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                                     >  2:834342/1‑140 (MQ=255)

                                                                          cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                                  <  1:845996/140‑1 (MQ=255)

                                                                          cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                                  <  2:1535169/140‑1 (MQ=255)

                                                                            tatTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCt                                                                <  2:4959959/140‑1 (MQ=255)

                                                                             tttttCAGGCTTTCTCCTCGTCCTTCTAAAAAATTTTCCTTTTCTGCATTTCCAGAATCC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                               <  1:3132205/139‑1 (MQ=255)

                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                               <  1:3292414/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTTCTTTTCTGAATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                          <  2:1791440/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                          <  2:2919412/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TCCC‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                          <  1:4781614/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                                <  2:4463063/117‑1 (MQ=255)

                                                                                    gtcGTTCTGCTCGTCCTTCTCAAAGAGTTTCCTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                        <  2:94104/138‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCATGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                        <  2:3931900/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                        <  1:1416334/140‑1 (MQ=255)

                                                                                       gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAGAC‑T‑‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTTAATAACTGAGCGTTTTTTGATAGTCTATTTCATTAGGt                                                    <  2:762041/140‑1 (MQ=255)

                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                                   <  2:522600/140‑1 (MQ=255)

                                                                                                 tCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatatat                                           <  1:4363496/140‑1 (MQ=255)

                                                                                                  ccTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATAAAtt                                          <  2:3907078/140‑1 (MQ=255)

                                                                                                       tcAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTaa                                     <  2:1634821/140‑1 (MQ=255)

                                                                                                           agagTTTACTTTTCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACaaa                                 <  1:3018556/140‑1 (MQ=255)

                                                                                                           agagTTTACTTTTCTGCATTTCCAGGATAC‑G‑‑C‑CCCCCCCATGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACaaa                                 <  1:815844/140‑1 (MQ=255)

                                                                                                                     tttCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                       <  1:4170599/140‑1 (MQ=255)

                                                                                                                     tttCTGCATTTCCAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa                       <  1:4993572/140‑1 (MQ=255)

                                                                                                                          gCATTTCAAGGATAC‑T‑‑CACCCCCCC‑TGACTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGaa                  <  2:120095/140‑1 (MQ=255)

                                                                                                                             tttCCAAGATACTT‑‑C‑CCCCACC‑TGGCTATTGTGCGCTCATACACTCCAATTAAAGATAGGTTCTAAATAAACGAGCGTTTTTTGTGAGTCTATTTCATTAGCTCATATATATTTGTAACAAATCAATCAAAATGGAATaa               <  2:4844309/140‑1 (MQ=255)

                                                                                                                                ccAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                                  <  2:2121324/86‑1 (MQ=255)

                                                                                                                                 cAGGATAC‑TC‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATc           <  1:393311/140‑1 (MQ=255)

                                                                                                                                  aGGATAC‑TC‑C‑CCCCCCC‑TGGCTAGTGTGCGCTCATACACTGAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCa          <  1:5130003/140‑1 (MQ=255)

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑T‑‑C‑CCCCCCC‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGCTACCA  >  NZ_CP025268/2466778‑2467054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`