BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	320,615	(G)_6→7	intergenic (+482/‑44)	rclR → / → ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	320,609	1	.	G	96.2%	861.8 / 23.7	266	intergenic (+476/‑50)	rclR/ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster
Reads supporting (aligned to +/- strand): ref base . (6/4); new base G (136/120); total (142/124)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGT‑GG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGt                                                                                                                                             >  1:4604788/1‑140 (MQ=255)

aGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGt                                                                                                                                             >  2:1500084/1‑140 (MQ=255)

  cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTCGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑CCCCCC‑GGT‑gg                                                                                                                                          <  2:2424543/140‑1 (MQ=255)

    aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGt                                                                                                                                             <  1:5355628/136‑1 (MQ=255)

     tGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGT‑gg‑gg‑g                                                                                                                                     >  2:106016/1‑140 (MQ=255)

             gaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACAt                                                                                                                            <  1:1098131/140‑1 (MQ=255)

             gaaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACAt                                                                                                                            <  2:4262446/140‑1 (MQ=255)

              aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATc                                                                                                                           <  1:1495793/140‑1 (MQ=255)

              aaaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATGAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATc                                                                                                                           <  1:4559537/140‑1 (MQ=255)

                 tacaaTTAGTTATTGCATTTGACGTTTGGATGAACGATTATCATTTGCCATACAAATGAGGGGTGGTATGTTGCTAGCCAGTTAACAAAGAACGCCATATTTATTGTTGATTGA‑TCCCCT‑GGTGGG‑GG‑G‑G‑AAACATCggg                                                                                                                        <  2:2698697/137‑1 (MQ=255)

                        aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAAt                                                                                                                 >  1:5327284/1‑140 (MQ=255)

                        aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAAt                                                                                                                 >  1:5394852/1‑140 (MQ=255)

                        aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAAt                                                                                                                 <  1:4628719/140‑1 (MQ=255)

                        aGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAAt                                                                                                                 <  2:4343713/140‑1 (MQ=255)

                         gTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATg                                                                                                                >  1:1841641/1‑140 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  2:2565896/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  1:2113314/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  2:2955611/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  1:728432/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  1:4118530/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            <  1:5334111/140‑1 (MQ=255)

                              tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCAt                                                                                                           >  2:4185729/1‑140 (MQ=255)

                               gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  1:4048612/140‑1 (MQ=255)

                               gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  2:1676236/140‑1 (MQ=255)

                                  tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAg                                                                                                       >  2:5451477/1‑140 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:1567388/140‑1 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:1314482/140‑1 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAAt                                                                                                   <  1:1060447/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  2:1636006/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAAAATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  1:1033508/140‑1 (MQ=255)

                                           tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                                                                              >  1:3582021/1‑140 (MQ=255)

                                           tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                                                                              >  1:1819591/1‑140 (MQ=255)

                                           tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                                                                              >  1:2921730/1‑140 (MQ=255)

                                            ggATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa                                                                                             <  2:5198490/140‑1 (MQ=255)

                                             gATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAt                                                                                            >  1:1156760/1‑140 (MQ=255)

                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                                                                          >  2:926905/1‑140 (MQ=255)

                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                                                                          <  2:1082822/140‑1 (MQ=255)

                                                                   tACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCtt                                                                      >  1:3082696/1‑140 (MQ=255)

                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  1:994386/140‑1 (MQ=255)

                                                                     cAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTg                                                                    >  1:243028/1‑140 (MQ=255)

                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  1:4923621/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:5087101/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:1694772/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:4102594/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTCATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGCGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:4465057/140‑1 (MQ=255)

                                                                         gggcgggTGGTCAGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATCATTGA‑TCCCCC‑GGT‑GGTGT‑T‑G‑AAACATCAGGCATAAATGTGCAGGTCGTAATTGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:5358803/136‑1 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt                                                            >  1:4807684/1‑140 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt                                                            >  2:484329/1‑140 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt                                                            >  2:378666/1‑140 (MQ=255)

                                                                               gtggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAt                                                          >  2:523331/1‑140 (MQ=255)

                                                                                tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAtt                                                         >  2:5206423/1‑140 (MQ=255)

                                                                                tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAtt                                                         >  1:3668521/1‑140 (MQ=255)

                                                                                aggtATGTTTCTAGCCAATTAACAAAGAACGCTATATTTATTGATAATTGATTCCCCT‑GCG‑GG‑GG‑G‑G‑AAACATCGTGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGACACCTGTAtt                                                         >  2:4804046/2‑140 (MQ=255)

                                                                                  gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  2:1713679/1‑140 (MQ=255)

                                                                                  gtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTgg                                                       >  1:44255/1‑140 (MQ=255)

                                                                                    aTGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTg                                                     >  1:4669308/1‑140 (MQ=255)

                                                                                    aTGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTg                                                     >  1:2893562/1‑140 (MQ=255)

                                                                                      gTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGAc                                                   <  2:1853914/140‑1 (MQ=255)

                                                                                       ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGT‑GG‑GG‑G‑GTAAACATCGGGCATAAATGGGCATGAAGTAATGGAGGATTAGTTGTGAATGTCAATTTCTTTGTAACCTGTATTGGTGACg                                                  >  1:867155/1‑140 (MQ=255)

                                                                                        tGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCa                                                                 >  2:1116810/1‑124 (MQ=255)

                                                                                         gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                >  2:1420981/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAGAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:5537176/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:3951558/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:4024300/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:2363658/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:2455391/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  2:1185424/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:2671962/1‑140 (MQ=255)

                                                                                            aGCCAATTAAAGAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGGACTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             >  1:4640220/1‑140 (MQ=255)

                                                                                            aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             >  2:3910572/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTTTCACCTGTATTGGTGACGCCCtta                                            >  2:2882943/1‑138 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:5089435/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:5548629/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  1:5303084/1‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGT‑GG‑GGAG‑G‑AAACATCGGGGATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTGCTTTGTCACCTGGATTGGTGACGCCCTGa                                            >  1:588736/1‑140 (MQ=255)

                                                                                                 aTTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATc                                        >  1:1978270/1‑140 (MQ=255)

                                                                                                  ttAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCa                                       >  2:1954533/1‑140 (MQ=255)

                                                                                                      aaaaGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  2:262932/140‑1 (MQ=255)

                                                                                                      aaaaCAACGCCATATTTCTTCATGACTGA‑TCCCCC‑GGT‑GG‑GG‑GCG‑AACCATCGGGCATACATGGGCATGAAGTCCCAGACTATTACTTGTGCATGTCACTTTCTTTGTCACCTGTATTGTTGCCGCCCTGAAATCCAGaa                                   <  2:3052984/140‑1 (MQ=255)

                                                                                                           aaCGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCa                              <  2:4732967/140‑1 (MQ=255)

                                                                                                           aaCGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATGAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCa                              <  1:4185729/140‑1 (MQ=255)

                                                                                                            aCGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTAAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCAc                             >  2:3437865/1‑140 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCa                                                                                                            >  2:3208426/1‑60 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  2:1819591/140‑1 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTAGTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            >  1:3416361/1‑140 (MQ=255)

                                                                                                                cATATTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgaga                         <  2:5394852/140‑1 (MQ=255)

                                                                                                                cATATTTATTGATGATTGA‑CCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgaga                         <  1:2562093/140‑1 (MQ=255)

                                                                                                                 atatTTATTGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGAc                        <  1:792458/140‑1 (MQ=255)

                                                                                                                  tatttattGATGATTGC‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAACTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt                       <  1:3100113/140‑1 (MQ=255)

                                                                                                                     ttattGATGATTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCg                    <  1:5556568/140‑1 (MQ=255)

                                                                                                                          gatgatTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               >  1:3510977/1‑140 (MQ=255)

                                                                                                                          gatgatTGA‑TCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               >  1:1432517/1‑140 (MQ=255)

                                                                                                                            tgattga‑tCCCCCGGGG‑GG‑GG‑G‑G‑AAACATCGGGCGTAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCTTTGAAATCAAGAATGGCACGAGACTCCGTgctgct             >  2:1566170/1‑140 (MQ=255)

                                                                                                                            tgattga‑tCCCCCGGGG‑GG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAGCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCAGTgctgct             >  1:2712144/1‑140 (MQ=255)

                                                                                                                              attgg‑tCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGACAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  2:5146900/1‑140 (MQ=255)

                                                                                                                               ttga‑tCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACt          >  1:516259/1‑140 (MQ=255)

                                                                                                                                tga‑tCCCCC‑GTTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTGATGGAGTATTAGTTGTGAATGTTAATTTCTTTGGCACGTGTACTGGTGATGCCCGGAAATAAAGAATAGCACGAGACTCCGTGCTGCTAcgg         >  2:4551126/1‑138 (MQ=255)

                                                                                                                                  a‑tCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGa       >  1:2845461/1‑140 (MQ=255)

                                                                                                                                  a‑tCCCCC‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGa       >  2:673946/1‑140 (MQ=255)

                                                                                                                                       ccc‑GGTGGG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGACATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGaaaaa   <  2:2109855/140‑1 (MQ=255)

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGA‑TCCCCC‑GGT‑GG‑GG‑G‑G‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`