BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,426,468	+C	pseudogene (249/275 nt)	yjfY ←	DUF1471 family protein YjfY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,426,468	1	.	C	98.5%	1096.1 / 6.7	329	pseudogene (249/275 nt)	yjfY	DUF1471 family protein YjfY
Reads supporting (aligned to +/- strand): ref base . (4/1); new base C (135/189); total (139/190)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

ATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCG‑T‑G‑A‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NZ_CP025268/4426329‑4426601

  ttccttccTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑gca                                                                                                                                       <  1:3850438/140‑3 (MQ=255)

   tccttcctCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑gcat                                                                                                                                      <  1:485304/140‑4 (MQ=255)

             ggAGACAACTGGTCTAATCCCGCCGCCGTTTTTCAAAAAAAAGCCCCATGCGAAGGGGGCAAAGCAAACTATAGCAATGTATCGTTGGTTATACCTGGTGCTAGCGATAAATTCCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTa                                                                                                                           <  2:4860102/140‑1 (MQ=255)

               agaTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCTAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACt                                                                                                                         <  2:5474766/140‑1 (MQ=255)

                 aTAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGACAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTcc                                                                                                                       >  2:4634174/1‑140 (MQ=255)

                   aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTcccc                                                                                                                     >  2:1030111/1‑140 (MQ=255)

                        gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTc                                                                                                                >  1:4069594/1‑140 (MQ=255)

                        gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTc                                                                                                                >  1:2964597/1‑140 (MQ=255)

                        gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑GATTGCCGTTACTCCCCGGTTc                                                                                                                >  1:198683/1‑140 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATCCCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:4777095/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTcc                                                                                                               <  2:593102/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:1414741/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:3604541/140‑1 (MQ=255)

                         tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:923494/140‑1 (MQ=255)

                             aTTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCa                                                                                                           <  1:4969550/140‑1 (MQ=255)

                              ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGTTTCCCGCAt                                                                                                          >  1:4282215/1‑140 (MQ=255)

                              ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                                          <  1:1524950/140‑1 (MQ=255)

                              ttCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCAATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCAt                                                                                                          <  1:3369569/140‑1 (MQ=255)

                                  gCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAAGATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGa                                                                                                      >  2:2327055/1‑140 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     >  1:2323933/1‑140 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     <  1:3831108/140‑1 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     >  1:2715609/1‑140 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     >  2:3002467/1‑140 (MQ=255)

                                   cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGaa                                                                                                     >  1:1070619/1‑140 (MQ=255)

                                     gCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAAtt                                                                                                   <  2:2749499/140‑1 (MQ=255)

                                      ccttgtatCAAAAAAAAGCACACCGCGAAGGGGGCAAAGCCTACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATACATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:1909531/133‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  1:2861187/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  1:2406750/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAAGATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:2844018/140‑1 (MQ=255)

                                      ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTCGTGCTAGCGATAAATATCCGCACTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:4934532/140‑1 (MQ=255)

                                      ccGTCTTCCGAAAAAAAGCCCCGTGCGAAGGGGGCAAAGCCAGCTATGGCAAGGTTTCGTTGGTTCTACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCG‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  2:2535425/140‑1 (MQ=255)

                                        gtgtttCAAAAAACAGCCCCCTGCGAAGGGGGCAAAGCAAGCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAGTATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:3649697/137‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAATCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:5139052/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:1901714/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:3210185/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:1325200/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:2752988/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:3303898/140‑1 (MQ=255)

                                        gTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  2:3850811/140‑1 (MQ=255)

                                          ttttCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTg                                                                                              <  1:3334652/140‑1 (MQ=255)

                                                    aaaGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGAGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGtt                                                                                    <  1:3070783/140‑1 (MQ=255)

                                                         ccccTGCGAAGGGGGCAAAGCAAACTATGGCGATGTTTCGTTGGTTACACCTGGTGCTAGCTATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTCCTCCCCGGTTCCCGCCTCAGAATTACGTGGTAGTACGTTGCGcc                                                                               <  2:4017224/140‑1 (MQ=255)

                                                            cTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTg                                                                            <  1:3128076/140‑1 (MQ=255)

                                                             tGCGAAGGGGTCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCCAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCTGCATCAGAATTACGTGGTAGTACGTTGCGCCTTgc                                                                           <  2:4771816/140‑1 (MQ=255)

                                                                       gcaaagcaaaCTATGGCAATGTGTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCGCCAGAATTACGCGGTAGGACGTTGGGCCTTGCGCAGCTGttt                                                                 >  1:2465392/1‑140 (MQ=255)

                                                                        caaagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttc                                                                >  1:2082739/1‑140 (MQ=255)

                                                                           atcaaaCTATGGCAACGTTTCGTTGGGTATACCGGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑G‑ACTATTGCCGTTATTCCCTGGTTCCCGCCTCAGAATTACGTTGTAGTACGTTGCGCCTTGCGCATGTGGgtcttc                                                             >  2:690104/3‑140 (MQ=255)

                                                                              aaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAtt                                                          >  1:5509404/1‑140 (MQ=255)

                                                                              aaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAtt                                                          <  1:1729281/140‑1 (MQ=255)

                                                                               aaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                                         <  1:3263602/140‑1 (MQ=255)

                                                                               aaCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                                         >  1:537322/1‑140 (MQ=255)

                                                                                aCTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTa                                                        <  1:5484444/140‑1 (MQ=255)

                                                                                         aTGTTGCGTTGGTGATACCTGGTGCTATCGATTAATATCCGCGCGGGCGCG‑G‑C‑TATTGCCGTTACTCGCCGGTTCCCGCATCAGAATTCAGAGGTAGTACGTTGGTCCTTGCGCATCTGTTGGCTCATTTATTGCCTGAc                                               >  2:5003270/1‑140 (MQ=255)

                                                                                          tGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACg                                              >  1:3429505/1‑140 (MQ=255)

                                                                                            tttCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGTCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTg                                            >  2:2661520/1‑140 (MQ=255)

                                                                                              tCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          <  1:118787/140‑1 (MQ=255)

                                                                                              tCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCt                                          >  1:2300139/1‑140 (MQ=255)

                                                                                                     ttATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCttt                                   >  1:3228898/1‑140 (MQ=255)

                                                                                                     ttATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCttt                                   <  1:4707859/140‑1 (MQ=255)

                                                                                                     ttATACCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCttt                                   <  1:3389546/140‑1 (MQ=255)

                                                                                                      tataCCTGGTGCTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc                                  >  2:5233103/1‑140 (MQ=255)

                                                                                                             ggTGCTAGCGATAAATATACGCGCTGGCG‑T‑GCA‑TATTGCCGTTCCTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCg                           <  2:1058221/140‑1 (MQ=255)

                                                                                                             ggTGCTAGCGATAAATATACGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCg                           <  2:3974363/140‑1 (MQ=255)

                                                                                                              gtgctAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:3393997/5‑140 (MQ=255)

                                                                                                              gtgctAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:846942/5‑140 (MQ=255)

                                                                                                              gtgctAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:1809376/5‑140 (MQ=255)

                                                                                                              gtgctAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  1:1574715/5‑140 (MQ=255)

                                                                                                              gtgcTAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATATGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGa                          >  2:3835158/1‑140 (MQ=255)

                                                                                                               tgctAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGaa                         >  2:1371194/4‑140 (MQ=255)

                                                                                                               tgctAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGaa                         >  1:2919466/4‑140 (MQ=255)

                                                                                                               tgctAGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGAGCCTTGCGCATCGGTGTCTTCATTTAATGCCTGACGTGCGTCGCTTTCAGTGGCGaa                         >  2:2150890/4‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:2190547/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:3502643/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:3806144/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:4539224/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:5266062/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:1149923/1‑140 (MQ=255)

                                                                                                                   aGCGATAAATATCCGCGCTGGCG‑T‑GCA‑TAGTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTGCATGTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:2617257/1‑140 (MQ=255)

                                                                                                                    gCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCAttt                                                         >  1:1014290/1‑103 (MQ=255)

                                                                                                                    gCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAt                    >  2:1741168/1‑140 (MQ=255)

                                                                                                                    gCGATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAt                    <  1:3643094/140‑1 (MQ=255)

                                                                                                                      gATAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtga                  <  1:535165/140‑1 (MQ=255)

                                                                                                                      gATAAATATACCCGCTTGCG‑TAC‑A‑TATTGCCGTTCCTCCCCGGTTCCCGCATCAGAATTACGTGGTCGTACGTTTCGCCTTGCCCATCTGTTTCTTCATTTAATGTCCGACGTGCTTCGCTTTTAGTGGCGAAATTAtga                  <  2:1201279/140‑1 (MQ=255)

                                                                                                                       cgaaaTATCCGCGCCGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCCTCAGAATTCCGTGGTAGTACGTTGCGCCTTGCGCCTCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGACATTAtgat                 <  2:4672353/138‑1 (MQ=255)

                                                                                                                       aTAAATATGCTCGCTGGCG‑T‑GCA‑TATTGCCGTTATTCCCCGGTTCCCGCATCAGACCTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTCCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  2:4089569/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:4022891/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 >  1:2555647/1‑140 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  2:5394162/140‑1 (MQ=255)

                                                                                                                       aTAAATATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  2:1942270/140‑1 (MQ=255)

                                                                                                                         aaaTATCCGCGCTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgattg               >  1:3277476/1‑140 (MQ=255)

                                                                                                                                 gcgcTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTTCGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       <  1:4660868/140‑1 (MQ=255)

                                                                                                                                 gcgcTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       <  1:4329808/140‑1 (MQ=255)

                                                                                                                                 gcgcTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       <  1:698002/140‑1 (MQ=255)

                                                                                                                                 gcgcTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       <  1:1877079/140‑1 (MQ=255)

                                                                                                                                 gcgcTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCACAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       <  1:5093633/140‑1 (MQ=255)

                                                                                                                                 ccgcTGGCG‑T‑GCA‑TCGAGCCGTTACTCCCCGGCGCCCGCATCAGAATTACGTGGTAGGACGTTGCGCCTTGCGCATCTGGTTCTTCCTTGATTGCATGACGTGCTTCGCTTTCCGTGGCGAAATTATGATTGATATGAAt       <  2:3132115/139‑1 (MQ=255)

                                                                                                                                  cgcTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc      >  2:1856094/1‑140 (MQ=255)

                                                                                                                                    cTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAc    >  2:4295053/1‑140 (MQ=255)

                                                                                                                                    cTGGCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCAc    >  2:1328733/1‑140 (MQ=255)

                                                                                                                                      ggCG‑T‑GCA‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc  >  2:3912000/1‑140 (MQ=255)

ATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCG‑T‑G‑A‑TATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NZ_CP025268/4426329‑4426601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`