BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	832,209	A→G	V336A (GTG→GCG)	ybhR ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	832,209	0	A	G	99.3%	906.8 / NA	277	V336A (GTG→GCG)	ybhR	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (158/117); total (159/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

CCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAA  >  NZ_CP025268/832072‑832345

cccggcGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTcgca                                                                                                                                        >  2:5133794/1‑140 (MQ=255)

    gcggTCGTGTCTATGCTGGCGAAAGATACGAAGTTACATCACCTTCCGTATACACATAGCATACGCAGCTGCACCTGTCGTGGCCGTTATCACCAGTAGCGGACACAAACCATACCACACAATATACAGACTCGCATCCt                                                                                                                                    <  2:4907110/140‑1 (MQ=255)

    gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAATATTGCGTACGCCGCTGACTCTGTCGTGGTCGTTATCACAAGTAGCGGTCACAAACTATTCCACACAATATCCAGAATCGCATCCt                                                                                                                                    >  2:1047762/1‑140 (MQ=255)

    gcggTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCt                                                                                                                                    >  1:4291225/1‑140 (MQ=255)

    gcggTACTGTCTCTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCAACAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCt                                                                                                                                    <  1:4671020/140‑1 (MQ=255)

         aGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaa                                                                                                                               <  1:2418166/140‑1 (MQ=255)

            gtCTTTGCTGGCGAAAGATACGAAGTTACATCACCTCACGTCTAAACATCGCGTACGCCGCTAACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataa                                                                                                                            <  2:4073824/140‑1 (MQ=255)

              cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  1:4224333/140‑1 (MQ=255)

              cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  2:3895006/140‑1 (MQ=255)

              cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  1:627573/140‑1 (MQ=255)

              cTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCaaataaat                                                                                                                          <  1:3368022/140‑1 (MQ=255)

                  tcTGGCGAAACATCAGAGCTTACATCACCTTACGTTTAAACTTCCCTTACTCCGCTGACCCTGTCGTTGCCGTTATCATCAGTAGCGGCCACAAACTATTCCACACAATATCCAGTCTCGCATCCTTCAAATAAATCGGc                                                                                                                      <  2:164593/139‑1 (MQ=255)

                  gCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                                                                      <  1:2653403/140‑1 (MQ=255)

                  gCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGc                                                                                                                      <  1:2155947/140‑1 (MQ=255)

                    tGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCATTCAAATAAATCTGCtt                                                                                                                    >  2:1124941/1‑140 (MQ=255)

                     ggCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTg                                                                                                                   >  2:2128531/1‑140 (MQ=255)

                         aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                                                                               <  1:5253997/140‑1 (MQ=255)

                         aaaGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTaa                                                                                                               <  2:1406423/140‑1 (MQ=255)

                                 gaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                       <  2:2661439/140‑1 (MQ=255)

                                 gaagTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTa                                                                                                       <  2:1793189/140‑1 (MQ=255)

                                    gTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAg                                                                                                    >  1:1908952/1‑140 (MQ=255)

                                      tACATCACCTTACGTCTAAACATCGCGTCCGCCGCTGACCCTGTGGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACGCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                                  <  2:5214335/140‑1 (MQ=255)

                                      tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                                  <  2:2946392/140‑1 (MQ=255)

                                      tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                                  <  1:901119/140‑1 (MQ=255)

                                      tACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTg                                                                                                  <  2:1740582/140‑1 (MQ=255)

                                       aCATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTgg                                                                                                 >  1:4016793/1‑140 (MQ=255)

                                           cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  1:2891598/140‑1 (MQ=255)

                                           cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  2:3304881/140‑1 (MQ=255)

                                           cACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAAGAAATCTGCGTGGTAATGTCCGTAAAGTGGCGaa                                                                                             <  2:270857/140‑1 (MQ=255)

                                            aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGCAt                                                                                            >  2:416725/1‑140 (MQ=255)

                                            aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            <  1:2124913/140‑1 (MQ=255)

                                            aCCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAAt                                                                                            >  1:412801/1‑140 (MQ=255)

                                                tACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAggg                                                                                        >  2:1945447/1‑140 (MQ=255)

                                                 aCGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGt                                                                                       >  1:1173853/1‑140 (MQ=255)

                                                 aCGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGt                                                                                       <  2:5560636/140‑1 (MQ=255)

                                                  cGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGtt                                                                                      >  1:2991260/1‑140 (MQ=255)

                                                     cTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAGCTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTACTGTCCGTAAAGTGGCGAATAGGGTTAAt                                                                                   >  2:754181/1‑140 (MQ=255)

                                                     cTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGCCTCGCATCCTTAAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                                                                   <  2:3103781/140‑1 (MQ=255)

                                                     cTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAt                                                                                   <  1:1731385/140‑1 (MQ=255)

                                                       aaaCATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATcc                                                                                 >  1:4422870/1‑140 (MQ=255)

                                                        aaCAGCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATGACCAGTAGCGGCCACAAACTATTGCACACAATATCCAGACTCGCAGCCGTCAAATAAATCTGCTTGGTAATATGCGGAAAGTTGCGAATAGGGGTAATCCa                                                                                >  2:443209/1‑140 (MQ=255)

                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCGCAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGACGAATAGGGTTAATCCACGTCa                                                                           >  2:4381286/1‑140 (MQ=255)

                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGCATAGGGTTAATCCACGTCa                                                                           <  1:4948062/140‑1 (MQ=255)

                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                                                                           >  2:567743/1‑140 (MQ=255)

                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCa                                                                           >  2:340704/1‑140 (MQ=255)

                                                             cgcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCAACGTCa                                                                           >  2:1252372/1‑140 (MQ=255)

                                                              gcgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAg                                                                          >  1:5057273/1‑140 (MQ=255)

                                                               cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAgg                                                                         >  1:3182185/1‑140 (MQ=255)

                                                               cgTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTGGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGACAgg                                                                         >  1:5100125/1‑140 (MQ=255)

                                                                  aCGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGttt                                                                      <  1:4745900/140‑1 (MQ=255)

                                                                                gTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCgg                                                        >  2:4974451/1‑140 (MQ=255)

                                                                                     ggCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGt                                                   >  1:960864/1‑140 (MQ=255)

                                                                                     ggCCGTTATCACCAGAAGCGGCCACAAACTGTTCCACACAATATGCAGACTCGCATCCTTCAAAAAAATCTACTTGGTAATGTCCGTAAAGTGGCGGATAGGGTTAGTCCAGGTCAGGTATTGCAGCAATACCGGTAttt                                                   >  2:2202917/1‑138 (MQ=255)

                                                                                       ccGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGttt                                                 >  1:2826713/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  1:163672/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  2:2507536/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  2:5437506/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  1:452705/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  1:705827/1‑140 (MQ=255)

                                                                                           tATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCa                                             >  1:363578/1‑140 (MQ=255)

                                                                                           tATCACCAGTACCGCCCACACACTATTCCACACAAAAACCACACTCGCATCCCTCAAATAAATCTGCTTGGTAATGTCCGTAAAGGGGCGAATAGGGTAAATCCACGTAAGGATATGCAGCAATACCGGCATGTTTTCCa                                             >  2:2836210/1‑140 (MQ=255)

                                                                                               aCCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCgg                                         >  2:2599877/1‑140 (MQ=255)

                                                                                               aCCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAAGCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCgg                                         >  2:4772161/1‑140 (MQ=255)

                                                                                                ccAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTCATCCACGTCAGGTTTTGCCGCCATACCGGCATGTTTTCCACCGga                                        <  2:4353982/140‑1 (MQ=255)

                                                                                                    tAGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGaaa                                    >  2:1552580/1‑140 (MQ=255)

                                                                                                     aGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAAc                                   <  1:3087939/140‑1 (MQ=255)

                                                                                                     aGCGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTAGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAAc                                   >  2:3991186/1‑140 (MQ=255)

                                                                                                       cGGCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGt                                 >  1:4856248/1‑140 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGCATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAACCGTaa                               >  2:4262875/1‑140 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               >  1:560810/1‑140 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               >  2:2344703/1‑140 (MQ=255)

                                                                                                         gCCACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTaa                               <  1:155599/140‑1 (MQ=255)

                                                                                                          ccACAAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAAc                              <  1:2721554/140‑1 (MQ=255)

                                                                                                           cacaAACTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGCAAcc                             >  2:3546765/1‑140 (MQ=255)

                                                                                                              aaaCTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                          <  1:5339409/140‑1 (MQ=255)

                                                                                                              aaaCTATTCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGa                          <  2:1871365/140‑1 (MQ=255)

                                                                                                                    ttCCACACAATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGgag                    >  2:303519/1‑140 (MQ=255)

                                                                                                                        acacaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATg                >  1:1192923/1‑140 (MQ=255)

                                                                                                                         cacaATATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATCGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATgg               >  1:5501077/1‑140 (MQ=255)

                                                                                                                            aagagccAGACTCGCATCCTTTAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCgg            <  2:4523110/135‑1 (MQ=255)

                                                                                                                            aaTATCCAGACTCGCATCCTTCAAATAAATGTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACGGGAAAGGAGAATGGCgg            >  1:3825490/1‑140 (MQ=255)

                                                                                                                            aaTATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCgg            >  2:5432826/1‑140 (MQ=255)

                                                                                                                            aaTATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCgg            <  1:3777635/140‑1 (MQ=255)

                                                                                                                            aaTATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCgg            >  1:2456118/1‑140 (MQ=255)

                                                                                                                            aaTATCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTCGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCgg            <  2:3423245/140‑1 (MQ=255)

                                                                                                                             atatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCggg           <  1:3526803/140‑1 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  1:1379277/1‑140 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  1:5189547/1‑140 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  1:1080127/1‑140 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  1:665397/140‑1 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  1:1838252/1‑140 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  2:3689601/1‑140 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  1:3739715/140‑1 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  1:1869726/1‑140 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  1:1936209/140‑1 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  1:2249954/140‑1 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          >  2:2249954/1‑140 (MQ=255)

                                                                                                                              tatCCAGACTCGCATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGc          <  1:2561888/140‑1 (MQ=255)

                                                                                                                                  cAGACTCGCATCCTTCAAAGAAACCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCCGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGcatca      <  2:5327053/140‑1 (MQ=255)

                                                                                                                                   acaCTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATCTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGcatcat     <  2:3460692/138‑1 (MQ=255)

CCCGGCGGTAGTGTCTTTGCTGGCGAAAGATAAGAAGTTACATCACCTTACGTCTAAACATCGCGTACGCCGCTGACCCTGTCGTGGCCGTTATCACCAGTAGCGGCCACAAACTATTCCACACAATATCCAGACTCACATCCTTCAAATAAATCTGCTTGGTAATGTCCGTAAAGTGGCGAATAGGGTTAATCCACGTCAGGTTTTGCAGCCATACCGGCATGTTTTCCACCGGAGAAACGTAACCGGAAAGGAGAATGGCGGGCATCATAAA  >  NZ_CP025268/832072‑832345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`