BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	833,226	T→C	378 (TAA→TAG)	ybhS ←	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	833,226	0	T	C	99.4%	1008.1 / ‑6.3	312	378 (TAA→TAG)	ybhS	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (135/173); minor base G (1/1); total (137/175)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

CGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGAC  >  NZ_CP025268/833090‑833360

cGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTaa                                                                                                                                     >  1:5367575/1‑140 (MQ=255)

    gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGTATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAGACATGCTCTTCTCCCTAATCCa                                                                                                                                 >  2:4381720/1‑140 (MQ=255)

    gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                 >  1:5160307/1‑140 (MQ=255)

    gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                 >  1:324093/1‑140 (MQ=255)

    gcggcgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCa                                                                                                                                 >  1:3550649/1‑140 (MQ=255)

        cgAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCg                                                                                                                             <  1:1793860/140‑1 (MQ=255)

            cGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGt                                                                                                                         <  2:1536510/140‑1 (MQ=255)

                   aGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGttt                                                                                                                  <  1:2085445/140‑1 (MQ=255)

                   aGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAAAGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGttt                                                                                                                  <  1:1287990/140‑1 (MQ=255)

                       tCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACTTTTGGTTTTCAg                                                                                                              <  2:5513333/140‑1 (MQ=255)

                        cACCTGAGTTAGCACGAGTAAAATCAGAATCGCGCGGGTTCGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTGACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCAAGCCGACGTTTGGTTTTCAGc                                                                                                             <  2:4636745/140‑1 (MQ=255)

                        cACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGc                                                                                                             <  1:5281451/140‑1 (MQ=255)

                         aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGTGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGcc                                                                                                            >  1:1201249/1‑140 (MQ=255)

                         aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGcc                                                                                                            >  2:4304518/1‑140 (MQ=255)

                         aCCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGcc                                                                                                            >  2:4719461/1‑140 (MQ=255)

                          ccTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCGATAAGCGATGAAACATGCTCTTCTCCCTCATCCAGCCGACGTTTGGTTTTCAGCCa                                                                                                           >  2:4821202/1‑140 (MQ=255)

                          ccTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCa                                                                                                           >  1:1833657/1‑140 (MQ=255)

                          ccTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCa                                                                                                           >  2:1904528/1‑140 (MQ=255)

                             gatgtAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTGGCGGATTAACGTCCAGAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                        <  1:1890451/136‑1 (MQ=255)

                             gAATTAGCACGTGTAAAATCAGAATCGCGCGGGTTTGCGGTTCCTGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCAATAAGATATGAAACATGCTCTCCTCCCTAACCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                        <  2:2924055/140‑1 (MQ=255)

                             gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGt                                                                                                        >  2:536613/1‑140 (MQ=255)

                             gAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGGTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAACCAcag                                                                                                        >  2:4773755/1‑138 (MQ=255)

                               aTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGTTTCGCGCAGCAACGACTGCAACTTTTTGCGGATTAACGTCCACAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTCCAGCCACGTCa                                                                                                      <  2:3349146/140‑1 (MQ=255)

                               aTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                      <  1:2263559/140‑1 (MQ=255)

                               aTTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCa                                                                                                      >  2:3670892/1‑140 (MQ=255)

                                 tAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAgg                                                                                                    <  1:3809607/140‑1 (MQ=255)

                                  aGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAAAGACTGCAACTCTTTGCAGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGc                                                                                                   <  2:2323334/140‑1 (MQ=255)

                                    cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACTACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCTACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                 >  2:1010018/1‑140 (MQ=255)

                                    cACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCg                                                                                                 >  1:650584/1‑140 (MQ=255)

                                     aCGGGTAAAATCAGAATCGCGCGGGTTTGTGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGACGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGa                                                                                                >  1:4625497/1‑140 (MQ=255)

                                     aCGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGa                                                                                                >  1:5186833/1‑140 (MQ=255)

                                        ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                             >  2:4570432/1‑140 (MQ=255)

                                        ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                             >  1:1974365/1‑140 (MQ=255)

                                        ggTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                             >  1:4223768/1‑140 (MQ=255)

                                        ggTAAAATCAGAATCGCGCGGGTTTGCGGATCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATaa                                                                                             >  1:82449/1‑140 (MQ=255)

                                            aaaTGAGGATCGGGCGGGTTTGCGGTTCGCGCAGCCACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCGGCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                         <  2:1811554/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                         <  1:5484913/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                         <  1:346871/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                         <  1:257640/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                         <  2:2176049/140‑1 (MQ=255)

                                            aaaTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACAt                                                                                         <  1:1356674/140‑1 (MQ=255)

                                              aTCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCa                                                                                       >  1:5387830/1‑140 (MQ=255)

                                                   aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCa                                                                                       >  2:3234460/1‑135 (MQ=255)

                                                   aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCAccgcc                                                                                  >  2:5471354/1‑140 (MQ=255)

                                                   aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCAccgcc                                                                                  >  1:884165/1‑140 (MQ=255)

                                                   aaTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCAccgcc                                                                                  >  1:102756/1‑140 (MQ=255)

                                                   aaTCGCGCGGGTGTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTCACGTCGATAAGCGATGAGACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGGTTTCAGCCACGTCAGGCCGATAAACATCAccgcc                                                                                  >  2:541710/1‑140 (MQ=255)

                                                    aTCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCAccgccg                                                                                 >  1:2154415/1‑140 (MQ=255)

                                                       gcgcgGTTGTGCGGTTCGCGCAGAAACGACTGCAACTCGTTGCGGATTAACGTGCATAAGCGGTGAGACGTGCTCTTCTCCCTATTGCAGCCGACGTGTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                              <  2:1241959/140‑1 (MQ=255)

                                                       gcgcgGGTTTGCGGTTCGGGCACCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCGTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                              <  2:3979151/140‑1 (MQ=255)

                                                       gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATATGCGATGAAACATGCTCTTCTCCCTAATCCAGCGGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                              <  2:1705278/140‑1 (MQ=255)

                                                       gcgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTGCTCCCTACTCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                              <  2:4487802/140‑1 (MQ=255)

                                                        cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAg                                                                              <  2:658729/139‑1 (MQ=255)

                                                        cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGc                                                                             >  2:3509643/1‑140 (MQ=255)

                                                        cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGc                                                                             >  1:2301984/1‑140 (MQ=255)

                                                        cgcgGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGc                                                                             >  2:2543602/1‑140 (MQ=255)

                                                                tGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCaaaa                                                                     >  1:2499326/1‑140 (MQ=255)

                                                                      tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                               <  2:4678142/140‑1 (MQ=255)

                                                                      tCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                               <  1:3720095/140‑1 (MQ=255)

                                                                      ccggccagcaACGACTGCAACTCTTTGCGGATTACCGTCCATAAGCGATGAAACATGCTCTTCTCCCTGATCCCGCCGACGTTTGGTTTTCAGCCACGTCAGGCCCATAAACAGCACCGCCGAAGCGATCAAAAACGGCa                                                               <  2:3595352/135‑1 (MQ=255)

                                                                      acgcgCACCAACGACTGCCACTCTTTGCGGATTAACGTGCCTAAGTGATGAAACAGGCTCTCCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCa                                                               <  2:1979280/139‑1 (MQ=255)

                                                                       cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                              >  1:2439454/1‑140 (MQ=255)

                                                                       cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                              >  1:1387687/1‑140 (MQ=255)

                                                                       cgcgCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCAc                                                              >  1:636490/1‑140 (MQ=255)

                                                                         cgcAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGt                                                            <  1:3509917/140‑1 (MQ=255)

                                                                           cagcaACGCCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGttt                                                          <  2:3579269/140‑1 (MQ=255)

                                                                           cagcaACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGttt                                                          <  2:324093/140‑1 (MQ=255)

                                                                              caACGACTGCAACTCCTTGCGAATTAACTTCCATACGCGATGAAACCTGCTCTTCTCCCTAACCCAGCCGCCGTTTGGTTTTCAGCCACGTCAGGCCGCTAAACCTCACCGCCGAAGCGATCAAACCCAGCACGTTTacc                                                       <  2:1323173/140‑1 (MQ=255)

                                                                                 cGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTaccacc                                                    >  2:4131817/1‑140 (MQ=255)

                                                                                   aCTGCAACTCTTTGCGGATTAATGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGATGACGTTTCGTTCTCACTCACCTCAGGCCCATAAACCTCACCCCCCAAGCGATCAAAAACAGCACGTTTACCACCAg                                                  <  2:3109135/140‑1 (MQ=255)

                                                                                   aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCCGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                  <  1:4880298/140‑1 (MQ=255)

                                                                                   aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                  <  1:2720206/140‑1 (MQ=255)

                                                                                   aCTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAg                                                  <  1:428817/140‑1 (MQ=255)

                                                                                     tgaaaCTCTGTGCGGATTAACGTCCATAAGCGATGACACATGCTCTTCTCCCTACTCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCCGca                                                <  2:4214059/137‑1 (MQ=255)

                                                                                        aaCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                             >  1:3082195/1‑140 (MQ=255)

                                                                                        aaCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcacca                                             <  1:1535301/140‑1 (MQ=255)

                                                                                         aCTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGcaccac                                            >  1:1110596/1‑140 (MQ=255)

                                                                                               tGCGGATTGACGTCCATAAGCGAGGAAACATGCGCTTCTCCCTAATCCAGCCGACGTTTGGGTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAACAACAGCACGTTTACCACCAGCACCACTGGCat                                      >  2:3949865/1‑140 (MQ=255)

                                                                                               tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAat                                      <  2:731788/140‑1 (MQ=255)

                                                                                               tGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAat                                      >  1:554710/1‑140 (MQ=255)

                                                                                                        aCGTCCATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGc                             <  2:1086616/140‑1 (MQ=255)

                                                                                                             cATAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGa                        <  1:1658244/140‑1 (MQ=255)

                                                                                                              aTAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGaa                       >  2:3927808/1‑140 (MQ=255)

                                                                                                               tAAGCGTTGAAACATGCTCGTCTCTCTTATCGACCCGACGTTTGGTTGTCAGCCACGTCAGGCCTATAAACTTCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACGAGCACCACTGGAATATTCCCGGCGAGGAAc                      <  2:3838432/140‑1 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAGCATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                      <  2:4083291/140‑1 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                      <  1:4916471/140‑1 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                      <  2:634889/140‑1 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                      <  1:3147091/140‑1 (MQ=255)

                                                                                                               tAAGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAAc                      <  1:3217160/140‑1 (MQ=255)

                                                                                                                aaGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACa                     <  1:1341599/140‑1 (MQ=255)

                                                                                                                 aGCGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCCCTGGAATATTCCCGGCGAGGAACAg                    <  2:2092584/140‑1 (MQ=255)

                                                                                                                  gCGATGAAACAGGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACTACTGGAATATTCCCGGCGAGGAACAgg                   <  2:2457451/140‑1 (MQ=255)

                                                                                                                   cGATGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGc                  <  1:3670892/140‑1 (MQ=255)

                                                                                                                     aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt                >  1:3856894/1‑140 (MQ=255)

                                                                                                                     aTGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCtt                >  1:4490241/1‑140 (MQ=255)

                                                                                                                      tGAAACATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCttt               >  1:3776917/1‑140 (MQ=255)

                                                                                                                         aaCATGCTCTTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa            <  1:5235533/140‑1 (MQ=255)

                                                                                                                         aaCATGCTCTTCTCCCTAACCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCa            <  2:5565585/140‑1 (MQ=255)

                                                                                                                               ctctTCTCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTGATGCCGATAAACACCACCGCCGCAGCTATCAAGTAGAGGGCGTTGTCCGCCGGCTCCACTGGAATGGTCCCGGAGAGGAGCTGGCTTTGCAGGGTGc      <  2:4999064/140‑1 (MQ=255)

                                                                                                                                 cttctCCCTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTg    <  1:4043934/140‑1 (MQ=255)

CGTGGCGGCGAACGGGAACAGGATCACCTGAATTAGCACGGGTAAAATCAGAATCGCGCGGGTTTGCGGTTCGCGCAGCAACGACTGCAACTCTTTGCGGATTAACGTCCATAAGCGATGAAACATGCTCTTCTCCTTAATCCAGCCGACGTTTGGTTTTCAGCCACGTCAGGCCGATAAACATCACCGCCGAAGCGATCAAAAACAGCACGTTTACCACCAGCACCACTGGAATATTCCCGGCGAGGAACAGGCTTTGCAGGGTGCTGAC  >  NZ_CP025268/833090‑833360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`