BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	920,456	A→G	L156L (TTG→CTG)	hcp ←	hydroxylamine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	920,456	0	A	G	99.2%	869.2 / NA	263	L156L (TTG→CTG)	hcp	hydroxylamine reductase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (189/71); minor base T (1/1); total (190/73)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

AAGAAGCGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACG  >  NZ_CP025268/920319‑920592

aagcagCGCTTTCATAGCGGCAGGCCAGGTCCCCAGCCACGTCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGGGCCATATAGGCCGCCGCACCTTTCAGGCCGTACAGGc                                                                                                                                        <  2:1111324/140‑1 (MQ=255)

     gcgcGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCAcagc                                                                                                                                   >  2:1928069/1‑140 (MQ=255)

        cgTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGa                                                                                                                                >  1:2223689/1‑140 (MQ=255)

          ttCTTATCGGCAGGCCAGGTCCCCCGCCTCGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACg                                                                                                                              <  2:5354141/140‑1 (MQ=255)

                   gCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCgag                                                                                                                     >  1:3879682/1‑140 (MQ=255)

                    cAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCgaga                                                                                                                    <  1:2376591/140‑1 (MQ=255)

                       gCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATg                                                                                                                 >  2:2044140/1‑140 (MQ=255)

                         cAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGtt                                                                                                               >  2:1962008/1‑140 (MQ=255)

                            gTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAACATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCTCCGCACCTTTCAGTCCAGACAGGCACAGCAGACGCAGGCCGAGAATGTTTTc                                                                                                            >  2:2608762/1‑140 (MQ=255)

                                 cAGTCACTCCATGATTTTATGTTACTGGGCATAAATTTAGTTGTCGTATTGACCGAGAACATGCGCTTTTTCTATATATGCGGCCGCACCTTTCATGCCATTCATTCACATCAGACGCTGGCCGAGCATGTTGTCGCCaa                                                                                                       >  2:2218670/1‑140 (MQ=255)

                                 cAGCCACGCCATGATTTTATGGTACTGTGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTCTTCGCCaa                                                                                                       >  1:691798/1‑140 (MQ=255)

                                 cAGCCACGCCATGATTTTATGGTACTGGGCATAGATATCGTTGTCGTATTGAGCGAGAACATGCGCGTGTTCCAGAGAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCGaa                                                                                                       >  2:1474246/1‑140 (MQ=255)

                                  aGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAAt                                                                                                      >  1:932231/1‑140 (MQ=255)

                                  aGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAAt                                                                                                      >  1:5476449/1‑140 (MQ=255)

                                  aGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAAt                                                                                                      >  1:5258267/1‑140 (MQ=255)

                                   gCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATc                                                                                                     <  1:1152462/140‑1 (MQ=255)

                                     cACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgc                                                                                                   <  2:2543096/140‑1 (MQ=255)

                                       ccccATGGTTTTCAGGTACTGGGCATAAATGCCGTTGGCGCAATACTCGAGACCATGCGCGTGTACCATATAGACGGCCGCGCCTATCAGCCTATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                                 >  2:4607084/3‑140 (MQ=255)

                                       cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                                 >  1:49228/1‑140 (MQ=255)

                                       cGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccg                                                                                                 >  1:4341768/1‑140 (MQ=255)

                                        gCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgccgc                                                                                                >  2:4225185/1‑140 (MQ=255)

                                             gATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTAt                                                                                           >  2:5050074/1‑140 (MQ=255)

                                              aTTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATc                                                                                          >  1:2086091/1‑140 (MQ=255)

                                                   aTGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGt                                                                                     >  1:1018727/1‑140 (MQ=255)

                                                    tGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGtt                                                                                    >  2:1786448/1‑140 (MQ=255)

                                                    tGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGtt                                                                                    >  1:1016087/1‑140 (MQ=255)

                                                     ggTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTa                                                                                   <  1:2599474/140‑1 (MQ=255)

                                                         cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAgggg                                                                               >  2:5322750/1‑140 (MQ=255)

                                                         cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAgggg                                                                               >  2:751696/1‑140 (MQ=255)

                                                         cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAgggg                                                                               >  2:5493205/1‑140 (MQ=255)

                                                         cTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAgggg                                                                               >  1:4471030/1‑140 (MQ=255)

                                                         cTGGGCATAAATATCGTTATCGTATTGACCGAGAACATGCGCGGGGTCCATATAGGGCGCCGCACCTTTCAGGCAATACAGGCACATCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAgggg                                                                               >  2:1660003/1‑140 (MQ=255)

                                                            ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  1:3394304/1‑140 (MQ=255)

                                                            ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  2:3456102/1‑140 (MQ=255)

                                                            ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  2:5572024/1‑140 (MQ=255)

                                                            ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTaa                                                                            >  1:4206681/1‑140 (MQ=255)

                                                            ggCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTGCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCTCAGCAGACGCAGGCCGTGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTGGGGGtca                                                                            >  2:5111992/1‑138 (MQ=255)

                                                            gacATAACTATCGTTGTCGTATTGACCGAGAACATGCGCGTGGTCCATATATGGCGCCGCACCTGTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATTTTTTCGCCAGTCGCCGCTATGTCTCTGTTAGGCGTaa                                                                            >  2:4039266/3‑140 (MQ=255)

                                                               aTAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAtt                                                                         <  1:4838973/140‑1 (MQ=255)

                                                                 aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                                       >  1:5602658/1‑140 (MQ=255)

                                                                 aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                                       >  1:2841834/1‑140 (MQ=255)

                                                                 aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                                       >  2:654504/1‑140 (MQ=255)

                                                                 aaaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTct                                                                       >  1:4756972/1‑140 (MQ=255)

                                                                  aaTATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTctg                                                                      <  2:4900448/140‑1 (MQ=255)

                                                                      tCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCtgctgc                                                                  >  1:393246/1‑140 (MQ=255)

                                                                      tCGTTGTCGTATTGACCGAGAACATGCGCGAGTTCCATATAGGCCGCCGCAACTTTCAGGCCATACAGGCACGGCAGACGCAGGCCGAGATTGTTTTGGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCtgctgc                                                                  >  1:3348099/1‑140 (MQ=255)

                                                                       cGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGcc                                                                 >  1:1446604/1‑140 (MQ=255)

                                                                       cGTTGTCGTATTGACCGAGAACATGCGCGCCGTCCATATAGGCCGCCACGCCGTTGAGTCAATACAGGCGCAGCAGACGCAGGCCGGGGATGTTTTCGCGAATCGCGGCAGTATCTGAGTTAGGGCTAAAGTCtgatgcg                                                                 >  2:3052663/1‑139 (MQ=255)

                                                                        gTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCACGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCt                                                                <  1:2658446/140‑1 (MQ=255)

                                                                         ttGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTg                                                               >  2:5340237/1‑140 (MQ=255)

                                                                           gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCgaga                                                                                                                    <  1:1899347/85‑1 (MQ=255)

                                                                           gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGAc                                                             >  1:2681130/1‑140 (MQ=255)

                                                                           gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGAc                                                             >  2:3806793/1‑140 (MQ=255)

                                                                           gTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGAc                                                             >  2:1870457/1‑140 (MQ=255)

                                                                            tCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGCGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACg                                                            >  1:4662079/1‑140 (MQ=255)

                                                                            tCGTATTGACCGAGAACATGCGCGGCTACCATATAGGCCGCCGCACCTGGCAGGCCGGACAGGCCCAGCCGCCGCTGGCCGAGTATGGTTTCGCCCATCGCGCCTCTCTCTTTGTTCGGGGTGGACTCTGCTGCCttaag                                                            >  2:4706188/1‑136 (MQ=255)

                                                                                         gaACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCACGCCg                                               >  2:866252/1‑140 (MQ=255)

                                                                                             aTGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAt                                           <  2:1374761/140‑1 (MQ=255)

                                                                                               gcgcGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcg                                         >  1:1705157/1‑140 (MQ=255)

                                                                                                  cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcgtcg                                      <  1:1414950/140‑1 (MQ=255)

                                                                                                  cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcgtcg                                      <  2:1262418/140‑1 (MQ=255)

                                                                                                  cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcgtcg                                      >  1:384727/1‑140 (MQ=255)

                                                                                                  cgTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGAtcgtcg                                      <  1:1961360/140‑1 (MQ=255)

                                                                                                      ttCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCAGAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCa                                  <  2:3266913/140‑1 (MQ=255)

                                                                                                      ttCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACTGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCa                                  <  1:5137470/140‑1 (MQ=255)

                                                                                                      ttCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCa                                  >  2:4440383/1‑140 (MQ=255)

                                                                                                      ttCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCa                                  >  2:2693895/1‑140 (MQ=255)

                                                                                                       tCCATATAGGCCGCCGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCAc                                 <  2:4071922/140‑1 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGc                                     >  1:514581/1‑127 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGc                                     <  2:514581/127‑1 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  1:2002133/1‑140 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  2:3113106/1‑140 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  2:5290035/1‑140 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  1:5527924/1‑140 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  1:746653/1‑140 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  1:4021815/1‑140 (MQ=255)

                                                                                                                gccgccGCACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCGCACCAGTTGCAg                        >  2:4547246/1‑140 (MQ=255)

                                                                                                                gccgccGCACCTTCCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAg                        >  1:4113689/1‑140 (MQ=255)

                                                                                                                   gccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTc                     >  2:3635045/1‑140 (MQ=255)

                                                                                                                   gccgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAAGTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTc                     >  2:3688109/1‑140 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATcgc                                                                                                   >  1:1041746/1‑60 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGt                      <  2:3894110/137‑1 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  2:157965/1‑140 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:18084/1‑140 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:2920777/1‑140 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:519128/1‑140 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  2:4060799/1‑140 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  2:4765109/1‑140 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  1:4542418/1‑140 (MQ=255)

                                                                                                                     cgcACCTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAg                   >  2:4325188/1‑140 (MQ=255)

                                                                                                                         ccTTTCAGGCCATACATGCACAGCAGACGCATGCCGAGAATGTTTTCGCCAATCGCCGCCTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGccgt               <  1:3119748/140‑3 (MQ=255)

                                                                                                                         ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  1:5384740/140‑1 (MQ=255)

                                                                                                                         ccTTTCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  2:221001/140‑1 (MQ=255)

                                                                                                                         ccTTTCAGGCCAGACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCAt               <  2:2761828/140‑1 (MQ=255)

                                                                                                                             tCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCggg           <  1:1834542/140‑1 (MQ=255)

                                                                                                                             tCAGGCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCggg           <  2:4341768/140‑1 (MQ=255)

                                                                                                                                 gCCATACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTAt       >  2:4221168/1‑140 (MQ=255)

                                                                                                                                    aTACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGa    >  1:3671619/1‑140 (MQ=255)

                                                                                                                                     tACAGGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCCGTTGCAGGTCAGCCATCGGGTTATCGAc   >  2:4113187/1‑140 (MQ=255)

                                                                                                                                      aCAGGCACAGCAGACGCAGGCCGAGAATGGGTTCGCCAATCGCCGCTTGATCTGTGGTGGGGGTAAATTTTGCTGCCTGACGGTGGAGCTCGCCGAGAGTGTCGCTCACTAGTTGCAGGTGCGCCGTCGGGTTagagagg  >  2:237202/1‑134 (MQ=255)

AAGAAGCGCGTTCATATCGGCAGGCCAGGTCCCCAGCCACGCCATGATTTTATGGTACTGGGCATAAATATCGTTGTCGTATTGACCGAGAACATGCGCGTGTTCCATATAGGCCGCCGCACCTTTCAGGCCATACAAGCACAGCAGACGCAGGCCGAGAATGTTTTCGCCAATCGCCGCTTTATCTTTGTTAGGGGTAAATTCTGCTGCCTGACGTTGCAGCTCGCCGAGATCGTCGCTCACCAGTTGCAGGTCAGCCATCGGGTTATCGACG  >  NZ_CP025268/920319‑920592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`