BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	973,690	A→G	755 (TAA→TAG)	ycaI →	ComEC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	973,690	0	A	G	99.7%	1124.7 / NA	342	755 (TAA→TAG)	ycaI	ComEC family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (189/152); minor base T (0/1); total (189/153)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 342 total aligned reads displayed.

AATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGT  >  NZ_CP025268/973554‑973826

     aTTGATACTCCACATCAAGTTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGGCTCAGGGAGCAAATTTTACCGCGGTGGTATCATCAGTGGTTTGGCTTGCTAGTGGATAACGGGTAGTATATGCg                                                                                                                                  >  2:14089/1‑140 (MQ=255)

     aTTGATACTCCACATCAAGGTCATACTACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAACTTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCg                                                                                                                                  <  1:2172758/140‑1 (MQ=255)

     aTTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGTCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCg                                                                                                                                  >  1:4428246/1‑140 (MQ=255)

     aTTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCg                                                                                                                                  >  2:2177949/1‑140 (MQ=255)

     aTTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCg                                                                                                                                  >  1:2548695/1‑140 (MQ=255)

     aTTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCg                                                                                                                                  >  1:2146804/1‑140 (MQ=255)

      ttGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCgg                                                                                                                                 <  1:4607601/140‑1 (MQ=255)

      ttGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCgg                                                                                                                                 <  1:3489236/140‑1 (MQ=255)

       tGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTGGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGc                                                                                                                                <  1:3362665/140‑1 (MQ=255)

         aTACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCCAGGCTGGAGGCTTAGCAGCCTCAGGGAGCAAATTTTACCCCGTTGGTGTCATCAGCGGTTTGGCGTGCCAGTGGATAACGGGTAGAATCTGCGGCtc                                                                                                                              >  2:1380911/1‑139 (MQ=255)

         aTACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTa                                                                                                                              >  2:399728/1‑140 (MQ=255)

          ttatCCATATCAAGGTCAAACCACGGTCAATTTTTCAGCGCATGGCTGGCGGTTTAGCAGCCGCATGGAGCAAATTTTACCTCGTTGGTATCATCAGTGTTTTGGCGTGCCAGTGGATAACGGTTAGAATATGCGGCTAt                                                                                                                             <  2:2694333/137‑1 (MQ=255)

          tACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTAt                                                                                                                             <  1:4054480/140‑1 (MQ=255)

            cTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTAttt                                                                                                                           >  1:3368461/1‑140 (MQ=255)

              ccACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTca                                                                                                                         >  1:3939835/1‑140 (MQ=255)

               cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTcaa                                                                                                                        >  2:647873/1‑140 (MQ=255)

               cacaTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTcaa                                                                                                                        >  2:2458264/1‑140 (MQ=255)

                 cctCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGCATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCCGCTATTTcaaca                                                                                                                      >  1:4235869/3‑140 (MQ=255)

                 caTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTcaaca                                                                                                                      >  2:2613853/1‑140 (MQ=255)

                  aTCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTcaacaa                                                                                                                     >  1:1014455/1‑140 (MQ=255)

                   tCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACaaa                                                                                                                    >  1:1631120/1‑140 (MQ=255)

                    cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAAt                                                                                                                   >  2:1361151/1‑140 (MQ=255)

                    cAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAAt                                                                                                                   >  1:3181360/1‑140 (MQ=255)

                     aaGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATg                                                                                                                  <  1:4832173/140‑1 (MQ=255)

                     aaGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATg                                                                                                                  <  2:3752781/140‑1 (MQ=255)

                       ggTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCt                                                                                                                >  1:287267/1‑140 (MQ=255)

                       ggTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCt                                                                                                                >  1:2399950/1‑140 (MQ=255)

                       ggTCAAACAACGGTCAATTTTCCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAGTTTTAACTCGTTGGTATCATCAGTGGTTTGGCGTGCCATTGGATAAAGGGCAGAATATGCGGATATTTCAACAAATGCt                                                                                                                >  2:4633389/1‑140 (MQ=255)

                           aaaCAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGtt                                                                                                            >  1:1835351/1‑140 (MQ=255)

                            aacaacGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGttt                                                                                                           >  1:323837/1‑140 (MQ=255)

                            aacaacGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGttt                                                                                                           >  1:662372/1‑140 (MQ=255)

                                 cGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGa                                                                                                      >  2:360392/1‑140 (MQ=255)

                                  ggTCAATTTTTCAGCGCAAGGCTGGCGGATTATCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGaa                                                                                                     >  1:5307086/1‑140 (MQ=255)

                                   gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAAt                                                                                                    >  2:2345390/1‑140 (MQ=255)

                                   gTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAAt                                                                                                    >  2:125662/1‑140 (MQ=255)

                                       aTTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCAt                                                                                                <  2:2982235/140‑1 (MQ=255)

                                           ttCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAACTGCTGGTTTTTTGAATGCATAACg                                                                                            >  1:1063876/1‑140 (MQ=255)

                                           ttCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACg                                                                                            >  1:2903185/1‑140 (MQ=255)

                                              aGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACa                                                                                         <  1:3866001/140‑1 (MQ=255)

                                                cgcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaaa                                                                                       <  2:1522244/140‑1 (MQ=255)

                                                cgcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaaa                                                                                       <  1:1716944/140‑1 (MQ=255)

                                                cgcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaaa                                                                                       <  2:3905047/140‑1 (MQ=255)

                                                cgcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACaaa                                                                                       <  1:5073395/140‑1 (MQ=255)

                                                 gcAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAg                                                                                      <  1:275992/140‑1 (MQ=255)

                                                   aaGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGAt                                                                                    <  1:3726650/140‑1 (MQ=255)

                                                          gCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTa                                                                             >  1:2059059/1‑140 (MQ=255)

                                                          gCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTa                                                                             >  2:5444979/1‑140 (MQ=255)

                                                          gCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTa                                                                             <  1:3266504/140‑1 (MQ=255)

                                                             gATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGt                                                                          <  2:4419024/140‑1 (MQ=255)

                                                                tAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTAGGCGTGCCAGTGGATAAAGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGc                                                                       <  1:4670754/140‑1 (MQ=255)

                                                                    agcCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGAc                                                                   >  1:5236077/1‑140 (MQ=255)

                                                                      ccTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACAt                                                                 <  1:4537384/140‑1 (MQ=255)

                                                                        tCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCGGTGGATAACGGGTAGAATATGCTGCTATTTGAACAAGTGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACaggc                                                               >  2:3459666/1‑137 (MQ=255)

                                                                        tCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTc                                                               >  1:793903/1‑140 (MQ=255)

                                                                        tCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTc                                                               >  1:4291820/1‑140 (MQ=255)

                                                                          aGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTccg                                                             >  1:1372794/1‑140 (MQ=255)

                                                                          aGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTccg                                                             >  2:5382529/1‑140 (MQ=255)

                                                                            ggAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTccgcc                                                           >  2:5285355/1‑140 (MQ=255)

                                                                               gCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGAc                                                        <  1:5555628/140‑1 (MQ=255)

                                                                                cAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACt                                                       <  2:4035002/140‑1 (MQ=255)

                                                                                cAAATTTTACCTCGTTGGTATCATCAGTGGTTGGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTAGTTCAACAAATGCGGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACt                                                       >  2:3080863/1‑140 (MQ=255)

                                                                                   aTTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACtgtg                                                    <  2:3645474/140‑1 (MQ=255)

                                                                                     tttACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTGCGCCGACTGTGGc                                                  >  2:4868167/1‑140 (MQ=255)

                                                                                     tttACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGc                                                  <  1:3435862/140‑1 (MQ=255)

                                                                                      ttACCTCTTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGcc                                                 >  2:4436034/1‑140 (MQ=255)

                                                                                             gttgttATCATCAGTGGTTTGGCGTGCCAGTGGATAACCGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCAtt                                          <  2:432287/140‑1 (MQ=255)

                                                                                             gTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCAtt                                          >  1:1115396/1‑140 (MQ=255)

                                                                                             gTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCAtt                                          >  2:5358486/1‑140 (MQ=255)

                                                                                             gTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCAtt                                          >  1:1955731/1‑140 (MQ=255)

                                                                                                ggTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTgcg                                       <  2:2903162/140‑1 (MQ=255)

                                                                                                ggTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGATGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGAATGTGGCCAACCATTgcg                                       <  1:3001148/140‑1 (MQ=255)

                                                                                                      atcaGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGCTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGAAGACATTCCGCCGACTGTGGCCAACCAGTGCGACTTTc                                 >  2:1608633/1‑140 (MQ=255)

                                                                                                         aGTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTAAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCaaa                              >  1:4352964/1‑140 (MQ=255)

                                                                                                          gcggTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAACGCATAACGACAAAGATCTCCCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  2:5482108/138‑1 (MQ=255)

                                                                                                          gTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  2:4757404/140‑1 (MQ=255)

                                                                                                          gTGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  2:1152505/140‑1 (MQ=255)

                                                                                                          gTGGTTTGGCCTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCCGGTTTTTTGAATGCATAACGACAAAGATCTCTCTCCGTGGCAGACATTCCGCCGATTGTGGCCAACCATTGCGCCTTTCAAAg                             <  2:4105722/140‑1 (MQ=255)

                                                                                                           tGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc                            >  1:1182523/1‑140 (MQ=255)

                                                                                                           tGGTTTGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGc                            >  2:1052820/1‑140 (MQ=255)

                                                                                                           cggTTTGGCGTGCCAGTGGAAAACGGGTAGAATATGCGGCTAATTCAACAAATGCTGGATTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACTAATGCGGCTTACAAAGc                            >  2:2553173/2‑140 (MQ=255)

                                                                                                              tttGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGCCATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCggg                         >  2:1771090/1‑140 (MQ=255)

                                                                                                              tttGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCggg                         >  1:1914635/1‑140 (MQ=255)

                                                                                                                tGGCGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTc                       >  1:2658669/1‑140 (MQ=255)

                                                                                                                   cGTGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGa                    >  2:809183/1‑140 (MQ=255)

                                                                                                                     tGCCAGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATc                  >  1:3650033/1‑140 (MQ=255)

                                                                                                                         aGTGGATAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTgg              <  1:2700261/140‑1 (MQ=255)

                                                                                                                               tAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg        <  1:919069/140‑1 (MQ=255)

                                                                                                                               tAACGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTggcgggcg        <  1:4476710/140‑1 (MQ=255)

                                                                                                                                aaCGGGTAGAATATGCGGCTATTTCAACACATGCGGGTTTTTTGAATGCATAAGGACAAAAATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       <  1:412407/140‑1 (MQ=255)

                                                                                                                                aaCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       <  2:3572111/140‑1 (MQ=255)

                                                                                                                                aaCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       <  1:5400968/140‑1 (MQ=255)

                                                                                                                                aaCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       >  1:3994859/1‑140 (MQ=255)

                                                                                                                                aaCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       <  1:3610019/140‑1 (MQ=255)

                                                                                                                                aaCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       >  1:2488194/1‑140 (MQ=255)

                                                                                                                                aaCGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGCGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGt       <  2:4982693/140‑1 (MQ=255)

                                                                                                                                  cGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             >  2:1438779/1‑116 (MQ=255)

                                                                                                                                  cGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAg                             <  1:1438779/116‑1 (MQ=255)

                                                                                                                                  cGGGTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTCGAATGCATAACGACAACGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCTTAg     >  2:1205485/1‑140 (MQ=255)

                                                                                                                                    ggTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCg   <  1:1045294/140‑1 (MQ=255)

                                                                                                                                     gTAGAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGt  <  2:2128418/140‑1 (MQ=255)

AATGGATTGATACTCCACATCAAGGTCAAACAACGGTCAATTTTTCAGCGCAAGGCTGGCGGATTAGCAGCCTCAGGGAGCAAATTTTACCTCGTTGGTATCATCAGTGGTTTGGCGTGCCAGTGGATAACGGGTAAAATATGCGGCTATTTCAACAAATGCTGGTTTTTTGAATGCATAACGACAAAGATCTCTCTACGTGGCAGACATTCCGCCGACTGTGGCCAACCATTGCGCCTTTCAAAGCGGGTCTGATCGTGGCGGGCGTAGCGT  >  NZ_CP025268/973554‑973826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`