BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,075,358	A→G	58 (TAA→TAG)	ymdF →	general stress protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,075,358	0	A	G	99.6%	923.1 / NA	280	58 (TAA→TAG)	ymdF	general stress protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (126/152); minor base T (0/1); total (127/153)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

AAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAA‑T‑CAT‑TTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NZ_CP025268/1075222‑1075491

aagaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCg                                                                                                                                        >  1:1680148/1‑140 (MQ=255)

  gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      <  1:1195104/140‑1 (MQ=255)

  gaCCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCggg                                                                                                                                      >  1:4886063/1‑140 (MQ=255)

     cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTa                                                                                                                                   >  2:1553819/1‑140 (MQ=255)

     cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGAGACAACTAGCCGGGCTa                                                                                                                                   >  2:4425153/1‑140 (MQ=255)

     cgcgAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGCAATTTCAAGAATGACCCGCAGCGCGCATCTGACCCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTACCCGGGCTa                                                                                                                                   >  2:1375930/1‑140 (MQ=255)

         aaaGAGCATCAGAAGAAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGGCACGGCAAAAGCGACAACTAGCCGGGCTAATCa                                                                                                                               <  2:2817227/140‑1 (MQ=255)

          aaGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCaa                                                                                                                              >  1:1486954/1‑140 (MQ=255)

                       gCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑tgcatt                                                                                                                >  2:1338011/1‑135 (MQ=255)

                        cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAGTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAt‑tt                                                                                                              <  2:5079739/140‑1 (MQ=255)

                          ggTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAt‑tttt                                                                                                            <  1:179140/140‑1 (MQ=255)

                                     tGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTc                                                                                                 >  2:1085998/1‑140 (MQ=255)

                                     gggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCTCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAATT‑CAT‑TTTTGTCTGTAGCTc                                                                                                 >  1:1987972/2‑140 (MQ=255)

                                      ggACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCg                                                                                                >  1:1322027/1‑140 (MQ=255)

                                      cgACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGGAGCTCg                                                                                                >  2:5420855/2‑140 (MQ=255)

                                            cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCaaaa                                                                                          >  1:3642445/1‑140 (MQ=255)

                                            cacagcGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCaaaa                                                                                          >  1:340795/1‑140 (MQ=255)

                                               accGGGCGTAATTAAAAAAAAGACCCGCAGCGCGCATCTGAAGCAGCCAAAAAAGGTGGTAAGAGTAGTCACGGCTAAAGCGACTACTAGCCGGGCTAATCAATGAAGAA‑TGCATGTTTAT‑ATGGAGCTGGTCAAAAAGc                                                                                       >  2:5073175/3‑140 (MQ=255)

                                                 cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCa                                                                                     >  2:1036524/1‑140 (MQ=255)

                                                 cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCa                                                                                     >  1:5469221/1‑140 (MQ=255)

                                                 cGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCa                                                                                     >  1:1549814/1‑140 (MQ=255)

                                                 cGGGGGGAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGAAAAAAAGGTGGTAAGAGCAGTCACGGCAATAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTGAAAAAGCCa                                                                                     >  2:548181/1‑140 (MQ=255)

                                                  gggggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCAt                                                                                    >  2:604454/1‑140 (MQ=255)

                                                    gggTAATTTCAAGAAGTACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCAGTAGCTCGTCAAAAAGCCATCa                                                                                  >  2:3917387/1‑140 (MQ=255)

                                                     ggaaaTTTCAAGAAAGACCCGCCGGGCGGATCAGAAACAGGTAAAAAAGGGGGAAAGAGCAGTCAAGGCAAAAGCGACAACTAGCCGGGCTAACCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCcaccac                                                                                 <  2:948394/137‑1 (MQ=255)

                                                     ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCAc                                                                                 <  1:2533769/140‑1 (MQ=255)

                                                     ggTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCAc                                                                                 <  1:1892665/140‑1 (MQ=255)

                                                      gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  1:3604581/1‑140 (MQ=255)

                                                      gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  2:456898/1‑140 (MQ=255)

                                                      gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  1:4504701/1‑140 (MQ=255)

                                                      gTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGGGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCAcc                                                                                >  1:909771/1‑140 (MQ=255)

                                                         aTTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCAccgcc                                                                             >  1:5291414/1‑140 (MQ=255)

                                                           ttCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCgg                                                                           <  1:816897/140‑1 (MQ=255)

                                                                gAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTAcc                                                                      <  2:2233317/140‑1 (MQ=255)

                                                                  aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAATAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:5215188/140‑1 (MQ=255)

                                                                  aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:3669996/140‑1 (MQ=255)

                                                                  aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:3426712/140‑1 (MQ=255)

                                                                  aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:908672/140‑1 (MQ=255)

                                                                  aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:2520967/140‑1 (MQ=255)

                                                                  aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  1:3338276/140‑1 (MQ=255)

                                                                  aTGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCg                                                                    <  2:5552958/140‑1 (MQ=255)

                                                                   tGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGAGAACTAGCCGGGCTAATCAATGAGGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAACAGCCATCACCGCCGGTTACCCgg                                                                   >  2:1189270/1‑140 (MQ=255)

                                                                     aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                 >  2:3592869/1‑140 (MQ=255)

                                                                     aCCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCggtg                                                                 >  1:3507804/1‑140 (MQ=255)

                                                                          cAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGa                                                            <  1:3741608/140‑1 (MQ=255)

                                                                            gcgcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATa                                                          <  2:3020933/140‑1 (MQ=255)

                                                                            gcgcgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATa                                                          <  1:4319366/140‑1 (MQ=255)

                                                                               cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAC‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtg                                                       >  1:1853893/1‑140 (MQ=255)

                                                                               cgcATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTGACCCGGTGGTGTATAcgg                                                       >  1:2312732/1‑138 (MQ=255)

                                                                                   tCTGAAGCAGGTACAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACtgatga                                                   <  2:1323548/140‑1 (MQ=255)

                                                                                    cTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGAc                                                  <  1:4820753/140‑1 (MQ=255)

                                                                                        aGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAAt                                              <  2:3153949/140‑1 (MQ=255)

                                                                                            ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTCGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  1:3129471/1‑140 (MQ=255)

                                                                                            ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  1:2198107/1‑140 (MQ=255)

                                                                                            ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  2:2309821/1‑140 (MQ=255)

                                                                                            ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  2:2733275/1‑140 (MQ=255)

                                                                                            ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACAGCCGGTTACACGGTGGTTGATACTGATGACAAATGTaa                                          >  2:2692941/1‑140 (MQ=255)

                                                                                            ggTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTCGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTaa                                          >  2:2450786/1‑140 (MQ=255)

                                                                                             gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         <  2:5139429/140‑1 (MQ=255)

                                                                                             gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         <  1:1640942/140‑1 (MQ=255)

                                                                                             gTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAg                                         <  2:451630/140‑1 (MQ=255)

                                                                                                   aaGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAGCCTGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGcc                                   <  1:4563554/140‑1 (MQ=255)

                                                                                                       tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                               >  1:4359736/1‑140 (MQ=255)

                                                                                                       tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                               >  1:1648445/1‑140 (MQ=255)

                                                                                                       tggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                               >  1:2968115/1‑140 (MQ=255)

                                                                                                       gggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGAt                               >  2:3551051/2‑140 (MQ=255)

                                                                                                        ggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                              >  2:145976/1‑140 (MQ=255)

                                                                                                        ggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                              >  1:5338623/1‑140 (MQ=255)

                                                                                                        ggtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATg                              >  2:3503647/1‑140 (MQ=255)

                                                                                                         gtAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgc                             >  1:2043996/1‑140 (MQ=255)

                                                                                                          tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:4769157/140‑1 (MQ=255)

                                                                                                          tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:3830040/140‑1 (MQ=255)

                                                                                                          tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:3210599/140‑1 (MQ=255)

                                                                                                          tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:3793758/140‑1 (MQ=255)

                                                                                                          tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:4329976/140‑1 (MQ=255)

                                                                                                          tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  1:4676992/140‑1 (MQ=255)

                                                                                                          tAAGAGCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATgcg                            <  2:1981497/140‑1 (MQ=255)

                                                                                                            agagCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAG‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGGAAGCTTGCCTGATgcgcc                          >  2:3999819/1‑139 (MQ=255)

                                                                                                            acagCAGTCACGGCAAAAGCGACAACTAGCCGGGCGAATCAATGACACA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATCcgcg                          <  2:5173061/138‑1 (MQ=255)

                                                                                                              agCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGAt                        <  1:4795523/140‑1 (MQ=255)

                                                                                                              agCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGAt                        <  2:4172306/140‑1 (MQ=255)

                                                                                                               gCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                       <  1:4242013/140‑1 (MQ=255)

                                                                                                               gCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                       <  1:5520781/140‑1 (MQ=255)

                                                                                                               gCAGTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATg                       <  1:848152/140‑1 (MQ=255)

                                                                                                                  gTCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCtt                    >  1:744330/1‑140 (MQ=255)

                                                                                                                   tCACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTa                   >  1:2879640/1‑140 (MQ=255)

                                                                                                                    cACGGCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTAt                  <  2:2019473/140‑1 (MQ=255)

                                                                                                                     aCGGCAAACGCGACAACTAGCCCGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGCCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGCTACTGATGCCAAATGTAAGCTTGCCTGATGCGCGATGCTTATc                 <  2:1930890/140‑1 (MQ=255)

                                                                                                                        gCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCggg              <  1:2780184/140‑1 (MQ=255)

                                                                                                                        gCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  1:2710103/140‑1 (MQ=255)

                                                                                                                        gCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  1:5055895/140‑1 (MQ=255)

                                                                                                                        gCAAAAGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAgg              <  1:3913549/140‑1 (MQ=255)

                                                                                                                          aaaaGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGcc            <  2:2638774/140‑1 (MQ=255)

                                                                                                                             aGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc         <  2:4634269/140‑1 (MQ=255)

                                                                                                                             aGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc         >  1:2466627/1‑140 (MQ=255)

                                                                                                                             aGCGACAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTAc         >  1:5218173/1‑140 (MQ=255)

                                                                                                                                  cAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa    <  2:1730461/140‑1 (MQ=255)

                                                                                                                                  cAACTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaa    <  1:4077089/140‑1 (MQ=255)

                                                                                                                                    aCTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  1:3050461/140‑1 (MQ=255)

                                                                                                                                    aCTAGCCGGGCTAATCAATGACGAA‑TGCAT‑TTTTGTCTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCagaaga  <  1:1097549/140‑1 (MQ=255)

AAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTGAAGCAGGTAAAAAAGGTGGTAAGAGCAGTCACGGCAAAAGCGACAACTAACCGGGCTAATCAATGACGAA‑T‑CAT‑TTTTG‑CTGTAGCTCGTCAAAAAGCCATCACCGCCGGTTACCCGGTGGTTGATACTGATGACAAATGTAAGCTTGCCTGATGCGCGATGCTTATCAGGCCTACCAGAAGA  >  NZ_CP025268/1075222‑1075491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`