BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,463,871	A→G	263 (TAA→TAG)	paaG →	2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase PaaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,463,871	0	A	G	100.0%	1169.9 / NA	351	263 (TAA→TAG)	paaG	2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase PaaG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (161/190); total (161/190)

Only 100 of 351 total aligned reads displayed.

GCGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAATC  >  NZ_CP025268/1463735‑1464007

gcgtTGAACTCAATACGCTCGATTCTCAACTGGATCTGGACCGTGACTATCAGCGGCTTCCCGGACTGAGCGCAGCTGCTCGTGAAGGTGCCAGTCCGTTCCTGGCTAAACGCTCACCGCAGTTCATCAGGAAATAGCat                                                                                                                                       <  2:4717982/140‑1 (MQ=255)

gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCat                                                                                                                                       <  2:1142680/140‑1 (MQ=255)

gcgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCat                                                                                                                                       <  1:380175/140‑1 (MQ=255)

 cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCGCCGCAGTTCACGGGGAAATAGCatg                                                                                                                                      >  1:5091046/1‑140 (MQ=255)

 cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCatg                                                                                                                                      >  2:1382153/1‑140 (MQ=255)

 cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCatg                                                                                                                                      >  2:3381318/1‑140 (MQ=255)

 cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCatg                                                                                                                                      >  1:2349796/1‑140 (MQ=255)

 cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCatg                                                                                                                                      >  1:1176125/1‑140 (MQ=255)

 cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCatg                                                                                                                                      >  2:5387689/1‑140 (MQ=255)

   cTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCatgat                                                                                                                                    >  1:1835562/1‑140 (MQ=255)

       aaCCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATa                                                                                                                                <  1:2484846/140‑1 (MQ=255)

        aCCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGGCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATaa                                                                                                                               <  1:4521080/140‑1 (MQ=255)

         ccAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATaaa                                                                                                                              <  2:605016/140‑1 (MQ=255)

         ccAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATaaa                                                                                                                              <  1:435880/140‑1 (MQ=255)

         ccAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATaaa                                                                                                                              <  2:257709/140‑1 (MQ=255)

             tACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAAtgtg                                                                                                                          <  2:2330254/140‑1 (MQ=255)

             tACGCGCGATACGCAACTGGATATGGAATGTGACTAGCAGCGACGGGCGGGACGGAGCGCAGAGTATCGTGAAGGGGTCAGTGCGTTCCTGGCTAAACGCGCACCGCAGTGCATGGGGAAGTTGCATGATGATAAAtgtg                                                                                                                          >  2:3245679/1‑140 (MQ=255)

               cGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCa                                                                                                                        <  2:3743362/140‑1 (MQ=255)

               cGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCa                                                                                                                        <  2:669276/140‑1 (MQ=255)

               cGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCa                                                                                                                        <  1:2266111/140‑1 (MQ=255)

               cGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCa                                                                                                                        <  1:3450317/140‑1 (MQ=255)

                  tCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAAc                                                                                                                     >  2:2122579/1‑140 (MQ=255)

                    gATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGAGAAATGTGCAAACtg                                                                                                                   >  2:1008119/1‑140 (MQ=255)

                       aagCAACTGGATCTGGGACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGAAAACTGTgg                                                                                                                <  2:3333434/138‑1 (MQ=255)

                         gCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCa                                                                                                              <  1:1353268/140‑1 (MQ=255)

                         gCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATCATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCa                                                                                                              <  1:4002192/140‑1 (MQ=255)

                               ggATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGAtt                                                                                                        <  1:238102/140‑1 (MQ=255)

                               ggATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGAtt                                                                                                        <  1:5579075/140‑1 (MQ=255)

                                gATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTg                                                                                                       >  1:532157/1‑140 (MQ=255)

                                    tGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                                   <  1:3332698/140‑1 (MQ=255)

                                    tGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                                   <  1:4568358/140‑1 (MQ=255)

                                    tGGAACGTGACTATCAGCGACGTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                                   <  2:4897011/140‑1 (MQ=255)

                                     ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGc                                                                                                  >  2:5224532/1‑140 (MQ=255)

                                     ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGGGATTGGGAGc                                                                                                  >  2:1792929/1‑140 (MQ=255)

                                       aaCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                                <  2:2742071/140‑1 (MQ=255)

                                          gTGACTATCAGCGACTTGCCGGACGGAGCGCAGCTTATCGTGAAGGTGTCAGTGCGTTTCGGGCTAAACGCTCACCGCAGTTCACGGGGAACTAGCATGATGATAAATGTGCAAACTGTGGCAGTGCTTGGGAGCGGCAc                                                                                             >  2:2007199/1‑140 (MQ=255)

                                           tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCGGTGATTGGGAGCGGCAcc                                                                                            >  2:1664099/1‑140 (MQ=255)

                                           tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAg                                                                                                             >  1:2187257/1‑123 (MQ=255)

                                            gACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCa                                                                                           >  2:1696331/1‑140 (MQ=255)

                                             aCTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt                                                                                          >  1:4136783/1‑140 (MQ=255)

                                             aCTATCAGCGAATTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCAt                                                                                          <  1:758719/140‑1 (MQ=255)

                                                   aGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggc                                                                                    >  1:3880620/1‑140 (MQ=255)

                                                     cGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGGGTCAGTGCGTTCCTGGCTAAACGCCAACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGTCAGTGATTGGGAGCGGCACCATGGGggcag                                                                                  >  2:3698335/1‑140 (MQ=255)

                                                      gACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCCTGATGCTAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                                 <  2:321027/140‑1 (MQ=255)

                                                      gACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                                 <  1:4199584/140‑1 (MQ=255)

                                                      gACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                                 <  2:2997611/140‑1 (MQ=255)

                                                           gCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTg                                                                            >  1:2972569/1‑140 (MQ=255)

                                                                     gcgcAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGGAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgc                                                                  <  2:2187743/140‑1 (MQ=255)

                                                                        cAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGTTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGCGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                               <  2:4370758/140‑1 (MQ=255)

                                                                        cAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                               <  1:4745809/140‑1 (MQ=255)

                                                                        cAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                               <  2:3918884/140‑1 (MQ=255)

                                                                        cAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgctgc                                                               <  2:4343821/140‑1 (MQ=255)

                                                                              aTCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCa                                                         >  1:5474855/1‑140 (MQ=255)

                                                                                cGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATTGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATg                                                       <  1:957619/140‑1 (MQ=255)

                                                                                cGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATg                                                       <  2:1479996/140‑1 (MQ=255)

                                                                                cGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATg                                                       >  1:2100456/1‑140 (MQ=255)

                                                                                cGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATg                                                       <  1:842286/140‑1 (MQ=255)

                                                                                cGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATg                                                       >  1:4952839/1‑140 (MQ=255)

                                                                                  tGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGa                                                     >  2:4197144/1‑140 (MQ=255)

                                                                                  tGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGa                                                     <  1:3634998/140‑1 (MQ=255)

                                                                                    agggagtCTGTGCGTTCATGGCTAAACGCTCACCGCCGTTTGCGGGGAAATAGCGCGATGCTACCTGTACAAACTGTGGGTGTGATTGGGAGCGGCACGGTGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                   <  2:1847081/135‑1 (MQ=255)

                                                                                    aaGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                   <  1:1614614/140‑1 (MQ=255)

                                                                                    aaGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                   <  1:1982180/140‑1 (MQ=255)

                                                                                    aaGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                   <  1:4423635/140‑1 (MQ=255)

                                                                                    aaGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                   <  1:3983964/140‑1 (MQ=255)

                                                                                      ggTGTCAGTGCGTTCCTGTCTAAACGCTCACCGCAGTTCACGGGGAAATTGCATGATGATAAATGTGCAAACTGTGGCAGTTATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACAcc                                                 <  2:3993459/140‑1 (MQ=255)

                                                                                      ggTGTCAGTGCGGTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATACATGTGCAAACTGTCGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACAcc                                                 <  2:3047738/140‑1 (MQ=255)

                                                                                       gtgtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCa                                                >  1:1435087/1‑140 (MQ=255)

                                                                                         gtCAGTGCGTTCCTGTCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGGGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAgg                                              >  2:2663207/1‑140 (MQ=255)

                                                                                         gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAgg                                              >  2:406295/1‑140 (MQ=255)

                                                                                          tCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGt                                             >  2:1324149/1‑140 (MQ=255)

                                                                                            aGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGttt                                           <  1:1530137/140‑1 (MQ=255)

                                                                                              tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTa                                         <  2:1447685/140‑1 (MQ=255)

                                                                                              tGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTa                                         >  2:82238/1‑140 (MQ=255)

                                                                                                 gTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTg                                      >  2:491487/1‑140 (MQ=255)

                                                                                                  ttCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCAGCCAGTCATGGACACCAGGATTTACTGt                                     >  1:5116262/1‑140 (MQ=255)

                                                                                                    ccTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTAt                                   <  1:2898303/140‑1 (MQ=255)

                                                                                                      tGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGGATGATGATAAAGGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGa                                 <  2:5245397/140‑1 (MQ=255)

                                                                                                        gCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACa                               >  2:1950119/1‑140 (MQ=255)

                                                                                                        gCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACa                               >  1:1305093/1‑140 (MQ=255)

                                                                                                        gCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACa                               >  1:1109772/1‑140 (MQ=255)

                                                                                                            aaCGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTc                           >  2:2901576/1‑140 (MQ=255)

                                                                                                             aCGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGAGTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTGACTGTATGACATTTct                          >  2:3559043/1‑140 (MQ=255)

                                                                                                                 tCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                      <  1:653103/140‑1 (MQ=255)

                                                                                                                 tCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                      <  1:5271743/140‑1 (MQ=255)

                                                                                                                 tCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                      <  1:188293/140‑1 (MQ=255)

                                                                                                                 tCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                      <  2:4615692/140‑1 (MQ=255)

                                                                                                                 tCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                      <  1:1777554/140‑1 (MQ=255)

                                                                                                                   aCCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGaa                    >  1:2077974/1‑140 (MQ=255)

                                                                                                                   aCCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGaa                    >  2:2397129/1‑140 (MQ=255)

                                                                                                                   aCCGCAGTTCACGGGGAAATAGCATGATGATAAATGAGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAGGTTGCTGCCCGGCATGGACACCAGGTTTGACTGTATGACATTTCTGCTGaa                    >  2:4447719/1‑140 (MQ=255)

                                                                                                                    ccGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAg                   >  1:492464/1‑140 (MQ=255)

                                                                                                                      gCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAgcg                 <  1:5410773/140‑1 (MQ=255)

                                                                                                                          ttCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCGGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATCTCTGCTGAAGCGCTGa             >  1:3099214/1‑140 (MQ=255)

                                                                                                                          ttCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCACGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGa             >  1:2660681/1‑140 (MQ=255)

                                                                                                                           tCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGAc            <  1:3215254/140‑1 (MQ=255)

                                                                                                                             aCGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGAccc          >  2:534083/1‑140 (MQ=255)

                                                                                                                                 ggAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCgcgc      >  1:1468039/1‑140 (MQ=255)

                                                                                                                                  gAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGGTACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCa     <  2:4395969/140‑1 (MQ=255)

                                                                                                                                    aaTAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAAt   >  2:3473831/1‑140 (MQ=255)

GCGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCAATC  >  NZ_CP025268/1463735‑1464007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`