BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,572,104	C→T	intergenic (‑66/+219)	CXP41_RS24370 ← / ← digH	protein YddY/glycoside hydrolase family 10 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,572,104	0	C	T	99.4%	598.3 / NA	160	intergenic (‑66/+219)	CXP41_RS24370/digH	protein YddY/glycoside hydrolase family 10 protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (84/75); minor base G (1/0); total (85/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

ACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCCACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAA  >  NZ_CP025268/1571967‑1572240

acCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTAc                                                                                                                                        >  2:640893/1‑140 (MQ=255)

  ccTTATAAGGCTGTGACATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTg                                                                                                                                      <  1:4593171/140‑1 (MQ=255)

  ccTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTg                                                                                                                                      <  2:1969585/140‑1 (MQ=255)

     tataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGttc                                                                                                                                   >  2:4910692/1‑140 (MQ=255)

     tataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGttc                                                                                                                                   >  2:1436800/1‑140 (MQ=255)

     tataAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGttc                                                                                                                                   >  2:1678845/1‑140 (MQ=255)

          ggCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCag                                                                                                                              <  1:815386/140‑1 (MQ=255)

           gCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCaga                                                                                                                             <  2:883399/140‑1 (MQ=255)

           gCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCaga                                                                                                                             <  2:5179185/140‑1 (MQ=255)

            cTGGTAAATCAGACGTAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAATTAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  2:1450808/140‑1 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTTCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  1:4519031/1‑140 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  2:3769504/140‑1 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  2:3943838/1‑140 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  2:140873/1‑140 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  1:913773/1‑140 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  1:5212978/140‑1 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  1:703457/140‑1 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  1:4394171/140‑1 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  1:4049520/1‑140 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  1:2580957/140‑1 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  1:2237234/1‑140 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            <  1:1953823/140‑1 (MQ=255)

            cTGGGAAATCAGACGGAATCAAAAGGAAACGCAACGGGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATGTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  2:539220/1‑140 (MQ=255)

            cTGGGAAATCAGACGGAAGCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaa                                                                                                                            >  1:3392138/1‑140 (MQ=255)

              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:1471873/1‑140 (MQ=255)

              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  2:3519801/1‑140 (MQ=255)

              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:2910776/1‑140 (MQ=255)

              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  2:3519028/1‑140 (MQ=255)

              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:4689424/1‑140 (MQ=255)

              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:4689241/1‑140 (MQ=255)

              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:1669376/1‑140 (MQ=255)

              gggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTGAGGACAGTTTCAATCTACGCTACTGTTCTTCagaaga                                                                                                                          >  1:1768997/1‑140 (MQ=255)

               ggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAagag                                                                                                                         >  2:2289578/1‑140 (MQ=255)

               ggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAagag                                                                                                                         >  1:2043114/1‑140 (MQ=255)

               ggAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAagag                                                                                                                         >  2:4951840/1‑140 (MQ=255)

                     cAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGc                                                                                                                   >  2:4966668/1‑140 (MQ=255)

                      aGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGcc                                                                                                                  <  1:4905436/140‑1 (MQ=255)

                        aCGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCa                                                                                                                <  2:766250/140‑1 (MQ=255)

                             aTCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTa                                                                                                           >  1:5335740/1‑140 (MQ=255)

                              tCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGACCATCGTaa                                                                                                          >  2:2678365/1‑140 (MQ=255)

                                      aaaCGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTAttttt                                                                                                  <  2:3632002/140‑1 (MQ=255)

                                            aaCGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGa                                                                                            >  2:1503836/1‑140 (MQ=255)

                                             aCGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGAc                                                                                           <  1:2945372/140‑1 (MQ=255)

                                              cGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCAGCGTAATTATTTTTCGGTGACa                                                                                          >  1:1461230/1‑140 (MQ=255)

                                                tGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGc                                                                                        >  2:4752809/1‑140 (MQ=255)

                                                tGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGc                                                                                        <  2:220825/140‑1 (MQ=255)

                                                  cGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      <  1:1704249/140‑1 (MQ=255)

                                                  cGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      <  1:4306837/140‑1 (MQ=255)

                                                  cGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      <  1:15905/140‑1 (MQ=255)

                                                  cGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      <  1:15790/140‑1 (MQ=255)

                                                   gagaTCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGaa                                                                                     <  1:2623329/140‑1 (MQ=255)

                                                     gaTCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAata                                                                                   <  2:4467190/140‑1 (MQ=255)

                                                     gaTCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAata                                                                                   <  1:3620098/140‑1 (MQ=255)

                                                          aCTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGACATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTa                                                                              >  2:4163735/1‑140 (MQ=255)

                                                          aCTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTa                                                                              >  1:47469/1‑140 (MQ=255)

                                                          aCTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTa                                                                              >  1:3866074/1‑140 (MQ=255)

                                                          aCTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTa                                                                              >  1:3325101/1‑140 (MQ=255)

                                                          aCTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTa                                                                              >  2:234634/1‑140 (MQ=255)

                                                                  cACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGttttt                                                                      <  1:3519801/140‑1 (MQ=255)

                                                                   aCCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttc                                                                     >  2:668086/1‑140 (MQ=255)

                                                                     cATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcat                                                                   >  1:3222215/1‑140 (MQ=255)

                                                                       tatTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatat                                                                 >  1:12517/1‑140 (MQ=255)

                                                                          tctcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattca                                                              >  2:564173/1‑140 (MQ=255)

                                                                          tctcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattca                                                              >  1:1015062/1‑140 (MQ=255)

                                                                          tctcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattca                                                              >  1:1014755/1‑140 (MQ=255)

                                                                            tcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGttttt                                                                      <  2:4334802/130‑1 (MQ=255)

                                                                            tcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattcata                                                            >  2:5292318/1‑140 (MQ=255)

                                                                            tcCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTttcatattcata                                                            >  2:5291929/1‑140 (MQ=255)

                                                                              cTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATGGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGATTTTCATATTCATACa                                                          >  1:3101706/1‑140 (MQ=255)

                                                                               tGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACAt                                                         >  1:2856865/1‑140 (MQ=255)

                                                                                gAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACAtt                                                        <  1:2289578/140‑1 (MQ=255)

                                                                                 aaaTATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      >  1:2018030/1‑109 (MQ=255)

                                                                                 aaaTATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGa                                                                                      <  2:2018030/109‑1 (MQ=255)

                                                                                 aaaTATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACAttt                                                       >  2:4474141/1‑140 (MQ=255)

                                                                                      tGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTttatt                                                  >  2:3770186/1‑140 (MQ=255)

                                                                                        aaGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAg                                                <  2:3325101/140‑1 (MQ=255)

                                                                                        aaGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTACTAg                                                <  2:4568502/140‑1 (MQ=255)

                                                                                                  gAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATgg                                      >  2:591236/1‑140 (MQ=255)

                                                                                                  aaaaaGCACTACGCGATTTAGGCCAGCTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCTCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATgg                                      <  1:3707598/139‑1 (MQ=255)

                                                                                                   aaaaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGc                                     >  1:492684/1‑140 (MQ=255)

                                                                                                     aaGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTg                                   >  1:4283855/1‑140 (MQ=255)

                                                                                                      aGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGt                                  <  1:1436396/140‑1 (MQ=255)

                                                                                                      aGAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTAAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGt                                  <  1:3584477/140‑1 (MQ=255)

                                                                                                       gAAATAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGtt                                 <  1:723482/140‑1 (MQ=255)

                                                                                                          aTAAGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCgg                                                                                               >  2:2509481/1‑75 (MQ=255)

                                                                                                            aaGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACt                            <  2:3584177/140‑1 (MQ=255)

                                                                                                            aaGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACt                            <  2:3387057/140‑1 (MQ=255)

                                                                                                             aGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTa                           >  1:2341356/1‑140 (MQ=255)

                                                                                                             aGCGATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTa                           <  1:1512895/140‑1 (MQ=255)

                                                                                                                gATTTAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTCACTAAgt                        <  2:3392138/140‑1 (MQ=255)

                                                                                                                  tttAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCTCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAgtgt                      >  2:3115460/1‑140 (MQ=255)

                                                                                                                   ttAGGACAGTTTCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGtgtg                     >  2:3259776/1‑140 (MQ=255)

                                                                                                                            tttCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTg            >  2:2645085/1‑140 (MQ=255)

                                                                                                                            tttCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTGTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTTGTTAATTTg            >  1:2303782/1‑140 (MQ=255)

                                                                                                                            tttCAATCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGGTTTTCATATTCATACATTTTTATTAGGTAGTTATGGCTGTTTAACTAAGTGTGATGAAGTTg            >  1:2303420/1‑140 (MQ=255)

                                                                                                                            tttCAATCTACGCGACTGTTCTTCAGCCGAGTATAGCCCATCGTCATTATTTTTCGGTGACAGCGAATATCGTATGGTTGTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGCGGTTAATTTg            >  2:5345279/1‑140 (MQ=255)

                                                                                                                                aaTCTACGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACtt        <  1:3829602/140‑1 (MQ=255)

                                                                                                                                      cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTTGTTAATTTGACTtaagtaa  >  1:275191/1‑140 (MQ=255)

                                                                                                                                      cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtatgtaa  >  2:36618/1‑140 (MQ=255)

                                                                                                                                      cGCTACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTtaagtaa  >  1:5279457/1‑140 (MQ=255)

ACCCTTATAAGGCTGGGAAATCAGACGGAATCAAAATGAAACGCAACGTGCGAGATCGACTAACTGCACCATATTCTCCTGAAATATGAAGATATACTGAAAAGAAATAAGCGATTTAGGACAGTTTCAATCTACGCCACTGTTCTTCAGAAGAGTATAGCCCATCGTAATTATTTTTCGGTGACAGCGAATATCGTATGGTTTTTCATATTCATACATTTTTATTAGGGATTTATGGCTGTTTAACTAAGTGTGGTTAATTTGACTTAAGTAA  >  NZ_CP025268/1571967‑1572240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`