BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,603,437	T→C	531 (TAA→TAG)	lsrK ←	autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,603,437	0	T	C	99.5%	709.6 / NA	216	531 (TAA→TAG)	lsrK	autoinducer‑2 kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (158/57); minor base G (1/0); total (159/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 216 total aligned reads displayed.

TTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGT  >  NZ_CP025268/1603301‑1603571

ttGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACtat                                                                                                                                     >  1:562986/1‑140 (MQ=255)

   aaTATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAAc                                                                                                                                  >  2:4137867/1‑140 (MQ=255)

        cGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAgg                                                                                                                             >  2:4908291/1‑140 (MQ=255)

        cGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAgg                                                                                                                             >  1:704542/1‑140 (MQ=255)

          tACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGgcg                                                                                                                           >  1:11393/1‑140 (MQ=255)

            cTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCt                                                                                                                         <  2:1471261/140‑1 (MQ=255)

               gTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTc                                                                                                                      >  1:4757675/1‑140 (MQ=255)

               gTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTc                                                                                                                      >  1:2513389/1‑140 (MQ=255)

               gTGATGGAACGATGAATCTGCAAGCTGGCTTCTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCtttg                                                                                                                      >  2:4350879/1‑139 (MQ=255)

                 gATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCa                                                                                                                    >  2:456800/1‑140 (MQ=255)

                  aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                                                                                                   >  1:2992900/1‑140 (MQ=255)

                  aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                                                                                                   >  2:3632650/1‑140 (MQ=255)

                  aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                                                                                                   >  1:5165829/1‑140 (MQ=255)

                  aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                                                                                                   >  2:1288046/1‑140 (MQ=255)

                  aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTGTCCAt                                                                                                                   >  2:910294/1‑140 (MQ=255)

                    ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATaa                                                                                                                 >  2:1239702/1‑140 (MQ=255)

                    ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATaa                                                                                                                 >  2:1722713/1‑140 (MQ=255)

                    ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATaa                                                                                                                 >  2:5145041/1‑140 (MQ=255)

                    ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATaa                                                                                                                 >  1:1046021/1‑140 (MQ=255)

                       aCGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacga                                                                                                              >  2:4714275/1‑140 (MQ=255)

                        ggATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgac                                                                                                             <  1:4926639/139‑1 (MQ=255)

                        agacaaaTATGCAAGCTGGCTTTTAACCAGCGAGCTCTGAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCTTGATGTGGCTGTCAATGAGACTATACCCCAGGCGCTTTCCATAacgac                                                                                                             <  2:231232/135‑1 (MQ=255)

                         gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgacg                                                                                                            >  2:2907291/1‑140 (MQ=255)

                         gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgacg                                                                                                            >  1:1007388/1‑140 (MQ=255)

                         gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgacg                                                                                                            >  2:4173972/1‑140 (MQ=255)

                         gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgacg                                                                                                            >  2:3085145/1‑140 (MQ=255)

                         gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTAGAACCCAGGCGCTTTCCATAacgacg                                                                                                            >  1:5108657/1‑140 (MQ=255)

                           tGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGAcgtc                                                                                                          >  1:2599322/1‑140 (MQ=255)

                             aaTCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                        <  2:5398727/140‑1 (MQ=255)

                             aaTCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGCAAACAAGAATAACTATACTCAAAACCTAACAGCCACGGTCATCATCATGTGGCTGTCAATGAAACTATAAGCCCGGCGCTTTCCATAACGAcgtcgt                                                                                                        <  1:3348758/140‑1 (MQ=255)

                                        tGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGAt                                                                                             <  1:2351914/140‑1 (MQ=255)

                                             tttAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAAcc                                                                                        <  2:4143777/140‑1 (MQ=255)

                                                     gCCAGCGCTAAAAGAAGGGAAATAAGAATAACTGTACGCAAAAACTAACAGCCACGGTCATGATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTGTTCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAg                                                                                >  2:2336433/1‑140 (MQ=255)

                                                          ctctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGAt                                                                           >  1:4731654/1‑140 (MQ=255)

                                                            ctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATACCCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATcc                                                                         <  2:1849510/140‑1 (MQ=255)

                                                               aaaGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGa                                                                      >  2:3063904/1‑140 (MQ=255)

                                                               aaaGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTGCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGa                                                                      >  2:1772294/1‑140 (MQ=255)

                                                                aagaagGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGAt                                                                     >  2:1987754/1‑140 (MQ=255)

                                                                   aagGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGAACAACCAGCCCCAGCTGATCCTGATaaa                                                                  <  2:4105374/140‑1 (MQ=255)

                                                                    agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGGCATCCAAAACGCCATCGCTAGCCCAAGCGCAACCATCCCGAGCTCAACCTGATAAAc                                                                 >  2:1055682/1‑140 (MQ=255)

                                                                    agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAc                                                                 >  1:3686737/1‑140 (MQ=255)

                                                                    agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAc                                                                 >  1:1848395/1‑140 (MQ=255)

                                                                    agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAc                                                                 >  2:2981730/1‑140 (MQ=255)

                                                                     gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAct                                                                >  1:80429/1‑140 (MQ=255)

                                                                     gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGAGCAACCAGCCCCAGCTGATCCTGATAAAct                                                                >  2:3923618/1‑140 (MQ=255)

                                                                      ggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActg                                                               >  1:363244/1‑140 (MQ=255)

                                                                       gAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                              <  1:3085145/140‑1 (MQ=255)

                                                                       gAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                              >  1:2231788/1‑140 (MQ=255)

                                                                         aaTAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgcct                                                            <  1:2944698/140‑1 (MQ=255)

                                                                         aaTAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgcct                                                            <  1:4276823/140‑1 (MQ=255)

                                                                                aTAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACtt                                                     >  1:4120680/1‑140 (MQ=255)

                                                                                 tAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTa                                                    >  1:3950696/1‑140 (MQ=255)

                                                                                       taCTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGt                                              >  1:2633983/1‑140 (MQ=255)

                                                                                       taCTCAAAAACTAACAGCCACGGTCATCATGATGCGGCTGTCATTGAAACAATAACCCAGGCGCTCTCCATAACGAGTTCGGCAGTCCATGATCAACCAGCCCAAGCTGATCCGGATAAACAGCATACAACTTATCCCGt                                              >  2:2808214/1‑140 (MQ=255)

                                                                                         cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                            >  2:3170941/1‑140 (MQ=255)

                                                                                         cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                            >  1:2156564/1‑140 (MQ=255)

                                                                                         cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                            >  1:1403562/1‑140 (MQ=255)

                                                                                           cAAAAACTAACAGCCATGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAAt                                          <  1:3096181/140‑1 (MQ=255)

                                                                                            aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCGAGCTGATCCTGATAAAGTGGCTGCCACGTATCGCGTGCATc                                         >  2:5091200/1‑140 (MQ=255)

                                                                                            aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                         >  2:3382896/1‑140 (MQ=255)

                                                                                            aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                         >  1:2204737/1‑140 (MQ=255)

                                                                                            aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                         >  2:2416117/1‑140 (MQ=255)

                                                                                            aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                         <  1:2852304/140‑1 (MQ=255)

                                                                                            aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                         >  2:4998483/1‑140 (MQ=255)

                                                                                                 cTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGAt                                    >  1:610762/1‑140 (MQ=255)

                                                                                                 cTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGAt                                    >  1:716195/1‑140 (MQ=255)

                                                                                                 cTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGAt                                    >  2:1561772/1‑140 (MQ=255)

                                                                                                            ggTCATCATGATGTGGCTGTCCATGAAACTATAACCCACGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCACCCCCAGCTGATCCTGATAAACTGCCTGTCACTTATCTCCTGAATCCTCATAGAGTTCATgg                         >  2:1385079/1‑139 (MQ=255)

                                                                                                            ggTCATCATGATGTGGCTGTCAATGAAACTATATCCCAGGCGCTTTCCATAGCGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCCGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAATTTCATGc                         <  2:4433156/140‑1 (MQ=255)

                                                                                                                 tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                    <  2:1134653/140‑1 (MQ=255)

                                                                                                                 tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                    <  2:3801841/140‑1 (MQ=255)

                                                                                                                 tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                    <  2:2672793/140‑1 (MQ=255)

                                                                                                                 tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                    <  1:4138342/140‑1 (MQ=255)

                                                                                                                 tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                    <  1:1618198/140‑1 (MQ=255)

                                                                                                                  catGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCAATAACGGCGCCGGCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATACACTGCCTGCCACTTATCTCGTGAATCCTGATAAATTTCATGCTTTTcc                   >  2:2091432/1‑140 (MQ=255)

                                                                                                                     gatgTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCggg                <  2:4849717/140‑1 (MQ=255)

                                                                                                                      atgTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGt               <  2:2860974/140‑1 (MQ=255)

                                                                                                                       tgtgGCTGTCAATGAAACTATAACCCAGGCGCTTTCGATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGGTAAACTGCCTGCCACGTATCGCGTGAATCCTTATAAAGTTCATGCGTTTCGGGGTc              >  2:3505855/1‑140 (MQ=255)

                                                                                                                       tgtgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgac                                                                                                             <  1:5465672/45‑1 (MQ=255)

                                                                                                                       tgtgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgac                                                                                                             >  2:5465672/1‑45 (MQ=255)

                                                                                                                        gtgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCt             >  1:153608/1‑140 (MQ=255)

                                                                                                                         tgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTg            <  2:3268828/140‑1 (MQ=255)

                                                                                                                          ggCTGTCAATGAAACTATAACCCAGGTGCTTTCCATAACGACGTCGTCAGTCCATGATGAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGTTAAAGGTCATGCTGTGCCGGGTCTgg           >  1:678900/1‑140 (MQ=255)

                                                                                                                          ggCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTACCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTgg           >  2:257442/1‑140 (MQ=255)

                                                                                                                           gCTGTCAATGAAACTATAACCCAGGCGCTTTGCATAAGGACGTCGACAGTCCATGAGCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCGCTTATCGCGTGAATCCTGATAACGTTCATGCTTTTCCGGGTCTgat          >  2:98227/1‑138 (MQ=255)

                                                                                                                           gCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATCAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgt          >  2:4524852/1‑140 (MQ=255)

                                                                                                                           gCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgt          >  1:2799005/1‑140 (MQ=255)

                                                                                                                            cTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtg         >  1:2033203/1‑140 (MQ=255)

                                                                                                                            cTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtg         >  2:1115668/1‑140 (MQ=255)

                                                                                                                            cTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtg         >  1:3589885/1‑140 (MQ=255)

                                                                                                                              gTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtgtg       >  2:4559354/1‑140 (MQ=255)

                                                                                                                              gTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtgtg       >  1:1434972/1‑140 (MQ=255)

                                                                                                                               tCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGttgtgt      >  2:4259549/1‑140 (MQ=255)

                                                                                                                               tCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtgtgt      >  1:918002/1‑140 (MQ=255)

                                                                                                                               tCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtgtgt      >  2:685570/1‑140 (MQ=255)

                                                                                                                                cAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGtgtgtg     >  2:1133593/1‑140 (MQ=255)

                                                                                                                                cAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGtgtgtg     <  1:3113692/140‑1 (MQ=255)

                                                                                                                                   tGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGCTCCTGATAAACTGCCTGCCACTTATCGCCTGAATCCTGATAAAGTTCATGCGTTTCCGGAGCTTgtgtgtcgtt  >  2:2468732/1‑136 (MQ=255)

                                                                                                                                   tGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGt  >  2:619750/1‑140 (MQ=255)

                                                                                                                                   tGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGt  >  1:305786/1‑140 (MQ=255)

TTGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGCGT  >  NZ_CP025268/1603301‑1603571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`