BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	160,613	T→C	235 (TAA→TAG)	sfsA ←	DNA/RNA nuclease SfsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	160,613	0	T	C	100.0%	786.2 / NA	234	235 (TAA→TAG)	sfsA	DNA/RNA nuclease SfsA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (132/102); total (132/102)

Only 100 of 238 total aligned reads displayed.

GGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCGCG  >  NZ_CP025268/160477‑160748

 ggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACa                                                                                                                                     <  1:5410157/140‑1 (MQ=255)

 ggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACa                                                                                                                                     <  2:2458655/140‑1 (MQ=255)

        tGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAAc                                                                                                                              >  2:1035160/1‑140 (MQ=255)

         gAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAAcc                                                                                                                             <  1:455091/140‑1 (MQ=255)

                cTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTg                                                                                                                      >  1:2835989/1‑140 (MQ=255)

                 tataAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGa                                                                                                                     <  1:2415051/140‑1 (MQ=255)

                        aGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTa                                                                                                              <  2:30322/140‑1 (MQ=255)

                        aGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTa                                                                                                              <  2:3779333/140‑1 (MQ=255)

                          cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                            >  2:5585885/1‑140 (MQ=255)

                           aGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAaga                                                                                                           >  2:625738/1‑140 (MQ=255)

                             aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                         >  1:5218842/1‑140 (MQ=255)

                             aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                         >  1:4051725/1‑140 (MQ=255)

                             aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                         >  1:2518911/1‑140 (MQ=255)

                               gCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCa                                                                                                       >  1:4802541/1‑140 (MQ=255)

                                cTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCAt                                                                                                      <  2:3662865/140‑1 (MQ=255)

                                            gCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGa                                                                                          <  1:4714586/140‑1 (MQ=255)

                                             cAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGaa                                                                                         >  2:3240662/1‑140 (MQ=255)

                                                  aTCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTc                                                                                    >  2:4520526/1‑140 (MQ=255)

                                                    cTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCg                                                                                  <  2:3741901/140‑1 (MQ=255)

                                                     tAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGGTTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGc                                                                                 >  1:1649314/1‑140 (MQ=255)

                                                       aaCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCtt                                                                               >  1:4575153/1‑140 (MQ=255)

                                                        aCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCtgt                                                                              >  1:2069192/1‑138 (MQ=255)

                                                             acacTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGATCGATGCATTCAGCAGAAATTTCCGCTTTGGAAg                                                                         >  2:2999210/1‑140 (MQ=255)

                                                             acacTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAg                                                                         >  2:1872629/1‑140 (MQ=255)

                                                             acacTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGCTACTTACTTGACTACAATGTAACCGGCAGTGAGTTTTTAAGAGTCATGCCTCCAGCAGAAATTGACGCTTTGTAAg                                                                         >  2:1459748/1‑140 (MQ=255)

                                                               acTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGcc                                                                       <  1:5066357/140‑1 (MQ=255)

                                                               acTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGcc                                                                       <  2:5113294/140‑1 (MQ=255)

                                                                 ttGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAg                                                                     >  1:226928/1‑140 (MQ=255)

                                                                  tGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGa                                                                    <  2:387933/140‑1 (MQ=255)

                                                                  tGACAACTGTGTGAGCAAAAGCGTATTCGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGa                                                                    <  2:3285114/140‑1 (MQ=255)

                                                                   gACAACTGTGTGAGGAAAAGCGTAGTTGCGCACGCGACCAGAATGTAAATTAAGCAGTTACTTACTTTACTACAATGTAACCGGCAGCGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCATTGTAAGCCAGaa                                                                   >  2:2740023/1‑140 (MQ=255)

                                                                        cTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCt                                                              >  2:2877136/1‑140 (MQ=255)

                                                                        cTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCt                                                              >  1:4547342/1‑140 (MQ=255)

                                                                        cTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCAGCTTTGTAAGCCAGAATTTCt                                                              >  2:5340846/1‑140 (MQ=255)

                                                                         tgtgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                             <  1:3596968/140‑1 (MQ=255)

                                                                         tgtgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTATACTACACTGTGACCTGCAGTGATTTTTTAAGAGCCAGGCCTTCAGCAGCAATTTCCGCTTTGTAAGCCAGAATTTCTa                                                             >  2:1223280/1‑140 (MQ=255)

                                                                         tctgtgAGTAACAGCGTATTTGCGCACGCGACCAGAATGTAAATTACCCAGTTACTTACTTTACTACACTGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGCATTTCTa                                                             <  2:692770/138‑1 (MQ=255)

                                                                           tgtgAGGAAAAGCGTCTTTGCGCACGCGACCAGACTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCGAGAATTTCTAcc                                                           >  2:667348/1‑140 (MQ=255)

                                                                           tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                           >  2:839788/1‑140 (MQ=255)

                                                                           tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                           >  1:343627/1‑140 (MQ=255)

                                                                           tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGATACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAcc                                                           >  2:2204366/1‑140 (MQ=255)

                                                                             tgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATGTCTAcccc                                                         >  1:4394972/1‑140 (MQ=255)

                                                                              gAGGAAAAGCGTATTTGCGCCCGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGCGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                                        <  1:1253196/140‑1 (MQ=255)

                                                                              gAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                                        <  1:4628849/140‑1 (MQ=255)

                                                                              gAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                                        <  1:1730556/140‑1 (MQ=255)

                                                                              gAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                                        <  1:2311775/140‑1 (MQ=255)

                                                                              gAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAccccc                                                        <  1:3274567/140‑1 (MQ=255)

                                                                                ggAAAAGCGGATTTGCGCAAGCGATCAGAATGTAAATTAACCAGTTACTTACTTGACTACAATGTAACCGGCAGTGATTTGGTAAGAGCCATGCCTTCAGCAGAAATTTCCGCGTTGTAAGCCAGAATTTCTACCCCCct                                                      <  2:3185382/140‑1 (MQ=255)

                                                                                     cgCGTATTTACGCACGCGACCAGAATGTAAACTAACCACTTCCTTACTTTACTACAATGTAACCGGCAGTGATAACTGAAGAGCTATGCCTTCATCAGAAATTTCCGCTTTGTAAGCCGGAACTTCTACCCCCCTCTGtt                                                 >  2:2470999/2‑140 (MQ=255)

                                                                                     aGCGTCTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  1:1434993/1‑140 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCGGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 <  1:2582893/140‑1 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACGGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  1:4086270/1‑140 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  1:763981/1‑140 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  1:109295/1‑140 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  2:5413288/1‑140 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  1:1679082/1‑140 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  2:4326340/1‑140 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  1:3863472/1‑140 (MQ=255)

                                                                                     aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGtt                                                 >  1:3704699/1‑140 (MQ=255)

                                                                                        gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAg                                              <  2:4181257/140‑1 (MQ=255)

                                                                                        gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAg                                              <  1:3952914/140‑1 (MQ=255)

                                                                                        gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTAACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTTTACCCCCCTCTGTTGAg                                              <  1:587002/140‑1 (MQ=255)

                                                                                          aTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCt                                            <  1:380071/140‑1 (MQ=255)

                                                                                          aTTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGGAAGCCAGAATTTCTACCCCCCTCTGTTGAGCt                                            >  2:3895096/1‑140 (MQ=255)

                                                                                           tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCtt                                           <  2:4303184/140‑1 (MQ=255)

                                                                                           tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCtt                                           <  1:4404436/140‑1 (MQ=255)

                                                                                           tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTAACCCCCTCTGTTGAGCtt                                           <  1:2250279/140‑1 (MQ=255)

                                                                                             tGCGCCCGCGACCAGAATGTACATTAACCAGTTCCTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCt                                         <  2:4956834/140‑1 (MQ=255)

                                                                                                    gcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATa                                  <  1:2948293/140‑1 (MQ=255)

                                                                                                     cgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAg                                 <  1:275624/140‑1 (MQ=255)

                                                                                                       aCCAGAATGTAAATTAAGCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGtt                               <  2:3834126/140‑1 (MQ=255)

                                                                                                       aCCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGtt                               <  1:3514702/140‑1 (MQ=255)

                                                                                                                tAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAtt                      >  2:2531839/1‑140 (MQ=255)

                                                                                                                tAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAtt                      >  2:1798585/1‑140 (MQ=255)

                                                                                                                 aaaTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGGCTTCAGCAAAAATTTCCGCTTTGTAAGCCAGAATTTCTAACCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAttt                     <  1:1989289/140‑1 (MQ=255)

                                                                                                                      gaCCAGTTACTTACTTTACTAAAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAGATTTCCGCTATGTAAGCCAGAATTTCTACCCCTCTCTGTTGAGCATCTGACAATAGCTGCGCGTAATTCTCAt                >  2:4634901/2‑140 (MQ=255)

                                                                                                                      aacgatttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCGGCTTTGTAAGCTAGAATTTCTACCCCCCTCTGTTGAGCTTATGAAAATAGTTTGGCGTATTTCTCac                >  2:116146/7‑139 (MQ=255)

                                                                                                                      aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                >  1:1227790/1‑140 (MQ=255)

                                                                                                                      aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                >  2:1368316/1‑140 (MQ=255)

                                                                                                                      aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                >  1:1956191/1‑140 (MQ=255)

                                                                                                                      aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                >  2:1404152/1‑140 (MQ=255)

                                                                                                                      aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                >  1:371490/1‑140 (MQ=255)

                                                                                                                      aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                >  2:5461911/1‑140 (MQ=255)

                                                                                                                      aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                >  1:2672664/1‑140 (MQ=255)

                                                                                                                          aGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACTCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCgtt            >  2:3422787/1‑138 (MQ=255)

                                                                                                                            ttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtgt          >  1:5553249/1‑140 (MQ=255)

                                                                                                                            ttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtgt          >  2:414496/1‑140 (MQ=255)

                                                                                                                              acttacttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCGACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCTATGTgg        >  2:2104585/1‑140 (MQ=255)

                                                                                                                                ttactttactACACTGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTGAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcg      <  2:5239761/140‑1 (MQ=255)

                                                                                                                                ttactttactACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcg      <  1:2236020/140‑1 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGGGCCATGCCTTCAGCAGAAGTTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTGGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    >  2:4833689/1‑140 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGCGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    <  2:4547342/140‑1 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    <  2:5087081/140‑1 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    <  2:40989/140‑1 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    <  2:16354/140‑1 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    <  1:1458455/140‑1 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    <  1:3373505/140‑1 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    <  1:4141012/140‑1 (MQ=255)

                                                                                                                                  acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg    <  1:5488412/140‑1 (MQ=255)

                                                                                                                                    tttACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGcgcgcg  <  2:2314215/140‑1 (MQ=255)

GGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCGCG  >  NZ_CP025268/160477‑160748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`