BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,166	T→C	K109R (AAG→AGG)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,166	0	T	C	98.7%	761.6 / NA	238	K109R (AAG→AGG)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (100/134); total (102/136)
Fisher's exact test for biased strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 238 total aligned reads displayed.

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCC  >  NZ_CP025268/1660029‑1660299

 aaaGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTAc                                                                                                                                         >  1:321279/1‑140 (MQ=255)

    gATCGAAAAAAGCCGGCGTGATTACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCGTACGTg                                                                                                                                      <  2:4956544/140‑1 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  1:553841/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  1:1995235/1‑140 (MQ=255)

     aTCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgc                                                                                                                                     >  1:2682338/1‑140 (MQ=255)

       cGAAAAAAGCCTGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                  >  1:255347/1‑140 (MQ=255)

       cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                  >  1:3985932/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCAGAAAAAAAGGCGGATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAAGGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                >  1:1358203/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAGAAAAGGGGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACTTTTTGGCAGCTGACACTTCCTCTTATCTTATTGATAATGTGTGTTTATGTTCTTAGGTTCG‑c‑c                                                                                                                                >  2:3172165/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                >  2:331772/1‑140 (MQ=255)

        gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                >  1:445760/1‑140 (MQ=255)

        gAAAAAAGCCGGAGAAATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACAGTTCATCTTATCGTATTGATAAAATGGATTTAGGTGCCGACGTgcgac                                                                                                                                  >  2:1311047/1‑138 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACATTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑ccagcc                                                                                                                       <  1:4358805/140‑6 (MQ=255)

               gCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑ccagcc                                                                                                                       <  2:4274595/140‑6 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCg                                                                                                                     <  1:1523658/140‑1 (MQ=255)

                 cGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCg                                                                                                                     <  2:2662298/140‑1 (MQ=255)

                    aGTAATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGcaa                                                                                                                  <  1:1161985/140‑1 (MQ=255)

                      tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGcaaca                                                                                                                <  1:2508670/140‑1 (MQ=255)

                      tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGcaaca                                                                                                                <  2:2707465/140‑1 (MQ=255)

                                  aaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATg                                                                                                    >  1:592802/1‑140 (MQ=255)

                                    ggCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGGCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGcc                                                                                                  >  1:1388406/1‑140 (MQ=255)

                                      cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGc                                                                                                <  2:3056498/140‑1 (MQ=255)

                                       gcatatTTGCGCTGTGAATGGTTGACAAAAGATGACATAGAATACCTTTTGTCAGCTGACACCTCCTCTTATCTTATTGATACACTGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCa                                                                                               >  2:3084297/3‑140 (MQ=255)

                                               gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                       >  2:719970/1‑140 (MQ=255)

                                               gcgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCaa                                                                                       >  1:4936563/1‑140 (MQ=255)

                                                cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑GGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                      >  2:1927479/1‑140 (MQ=255)

                                                    gtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAAc                                                                                  >  1:1641764/1‑140 (MQ=255)

                                                     tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGGGCAATCAACg                                                                                 <  1:2947502/140‑1 (MQ=255)

                                                       aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                               <  1:4948452/140‑1 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                              <  1:2102548/140‑1 (MQ=255)

                                                        aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑ACCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                              <  2:5113124/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGCATACCTTTTGTCAGCTGCCACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  1:2289720/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  2:3588189/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  2:1580612/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  1:2207416/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  2:1831165/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  1:2900109/140‑1 (MQ=255)

                                                             tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGCGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                         <  2:3987067/140‑1 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGcaaca                                                                                                                >  1:1092015/1‑99 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  1:2760666/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  2:153817/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  2:367365/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  1:187285/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  1:156752/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  1:5399699/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  2:726018/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  2:766384/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  1:3795916/1‑140 (MQ=255)

                                                               aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                       >  1:1833348/1‑140 (MQ=255)

                                                                   aaGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCa                                                                   >  2:3649647/1‑140 (MQ=255)

                                                                         aaaTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAaga                                                             <  1:1697006/140‑1 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                           <  2:4508759/140‑1 (MQ=255)

                                                                           aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                           <  1:3917377/140‑1 (MQ=255)

                                                                                  aCCTTTGGTCAGCTGACACTTACTCTAATCTTATTGTTAAACTCGATTTATGTCCCTACGTGCGCC‑C‑C‑C‑CGCCCGCTACCATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                    <  2:241589/140‑1 (MQ=255)

                                                                                    cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCAc                                                  >  2:3134966/1‑140 (MQ=255)

                                                                                     ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                 <  1:4195781/140‑1 (MQ=255)

                                                                                      tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                <  2:2339534/140‑1 (MQ=255)

                                                                                       ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                               <  1:558277/140‑1 (MQ=255)

                                                                                         gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                             >  1:3595527/1‑140 (MQ=255)

                                                                                             gCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcac                                         <  1:5175806/140‑1 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                        >  1:3017015/1‑140 (MQ=255)

                                                                                              cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGGCAGTTTCACGCCATCcaca                                        >  1:3684389/1‑140 (MQ=255)

                                                                                              cTGACACTTCCTCTTAGCGTATTGATAAAATGGATTTAGGTGCCTACGTGCGCC‑C‑C‑C‑GGCACGCAACAATGATCAACATGCCGCAAACCGCTATCACCGCACACGTCCAGTTACAAAGAGTCAGTTTCACGCCATCccca                                        <  2:4376783/140‑1 (MQ=255)

                                                                                               tGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c‑c‑cca                                                                                                                          >  2:71429/1‑55 (MQ=255)

                                                                                                       cctcctATCTTAGTAACATAATGGATTTATGATCTTACTAACG‑CAC‑C‑C‑AGTCAGAAACAATTAACAACATACCAAAAAGCGCAATCAACGCACCCGTCCAGTCCTAAAGAGTCAGTTCCACGCCATCCACCACGCGCAGc                               <  2:1820274/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTCTTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATTATCAACATGCCGCAAAGCGCAATCATCGCACCCGTCGAGTCATTAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  2:1933417/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATTGATTTATGTTCCTATGTGCG‑C‑C‑CAC‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGAAGc                               <  2:5576456/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  2:3443657/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  2:4608221/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  2:950359/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  2:3270173/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  1:213324/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  1:3134966/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  1:4740016/140‑1 (MQ=255)

                                                                                                       ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               >  2:1091774/1‑140 (MQ=255)

                                                                                                       ccTCTGATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGGCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                               <  2:2634479/140‑1 (MQ=255)

                                                                                                             atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                         >  1:814321/1‑140 (MQ=255)

                                                                                                             atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                         >  2:5338286/1‑140 (MQ=255)

                                                                                                             atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                         >  2:1397248/1‑140 (MQ=255)

                                                                                                                 tatTTAGAAAATGGTTTTATGTTCCTACGTGCG‑C‑C‑C‑CTAGCCCTCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTGCACGCCATCCACAACGTTCATCCACATCAACg                     <  2:377248/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAGCGCGCAGCCACATCAACg                     <  2:561579/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                     <  1:2345358/140‑1 (MQ=255)

                                                                                                                 tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                     <  2:4936563/140‑1 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                    >  2:2052824/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                    >  1:2392191/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                    >  2:4210373/1‑140 (MQ=255)

                                                                                                                  atTGATAAAATGGATTTATGTTCCTACGTGCG‑C‑CAC‑C‑AGCCCGCAACAATGATTAACATGCCGCAAAGCGCAATCAACCCACCCGTCCAGTCATAAAGAGTCAGTTACACGCCATCCACAACGCGCAGCCACATCAACGc                    >  1:938191/1‑140 (MQ=255)

                                                                                                                   ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                   >  2:1609981/1‑140 (MQ=255)

                                                                                                                      aTAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                <  2:4152006/140‑1 (MQ=255)

                                                                                                                       tAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGc               <  1:508685/140‑1 (MQ=255)

                                                                                                                       tAAAATGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGc               <  1:541631/140‑1 (MQ=255)

                                                                                                                        aaaaTGGATTTATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa              <  2:553841/140‑1 (MQ=255)

                                                                                                                                tttATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa      <  1:4220979/140‑1 (MQ=255)

                                                                                                                                tttATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa      <  2:4034472/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  1:5138923/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  2:5439195/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  1:331772/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg    <  2:756939/140‑1 (MQ=255)

                                                                                                                                  tATGTTCCTACGTGCGAC‑C‑C‑C‑AGCACGCAACTATGATCAACATGCTGCAAAGCGCAATCAACGCACCCGTCCAGTCATACAGAGTCAGTTTCACGCCATCCACAAAGCGCAGGCACATCAACGCCGTGCAGACATAAACg    <  2:916649/140‑1 (MQ=255)

                                                                                                                                    tGTTCCTACGTGCGCC‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGcc  >  1:2824167/1‑140 (MQ=255)

TAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑C‑C‑C‑AGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCC  >  NZ_CP025268/1660029‑1660299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`