BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	14,302	T→C	V45A (GTG→GCG)	dnaJ →	molecular chaperone DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	14,302	0	T	C	99.6%	917.6 / NA	279	V45A (GTG→GCG)	dnaJ	molecular chaperone DnaJ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (146/132); minor base G (1/0); total (147/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

ATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTT  >  NZ_CP025268/14169‑14437

aTGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAtt                                                                                                                                   >  1:4815457/1‑140 (MQ=255)

    cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                               <  1:504328/140‑1 (MQ=255)

    cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                               <  2:503219/140‑1 (MQ=255)

       aGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAagag                                                                                                                            <  2:1529866/140‑1 (MQ=255)

             attattACGAGATTTTAGGCGTTTCCAAAACAGCGGGAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                      >  2:1402667/1‑140 (MQ=255)

             attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                      >  2:2519462/1‑140 (MQ=255)

             attattACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAg                                                                                                                      >  1:1499787/1‑140 (MQ=255)

               tattaCGATATTTTATGCGTTGCCAAAACGCCGGACGAGACCGAAACCAGAATGGCCTACACACCCGTGGCCATTGAATACCACCGGGACCGTAACCAGGGTGACAAAGAGGCCCAGGCGGAATTTATGGATATCAAGGa                                                                                                                    <  2:1098256/140‑1 (MQ=255)

                   aCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAGGAGATCAAGGAAGCt                                                                                                                >  2:5293097/1‑140 (MQ=255)

                      agaTTTTAGGCGTTCCAACAACAGCGGCAGAGCGTGAAATCAGAACGGCCTCCAAAAGCCTGGCCATGCAATACCACCCGGCCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                                             <  2:1617824/140‑1 (MQ=255)

                       gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                            <  2:5194751/140‑1 (MQ=255)

                       gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGGGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                            >  1:696888/1‑140 (MQ=255)

                                  tttCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGAcc                                                                                                 >  2:2613822/1‑140 (MQ=255)

                                   ttCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCg                                                                                                >  1:1378829/1‑140 (MQ=255)

                                    tCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGa                                                                                               >  2:3758080/1‑140 (MQ=255)

                                              cGGAAGGGCGTGAAATCAGAAAGGCCTACAAACGCCTTGCGATGAAATACTACCTGTACCGTAAGCAGCGGGACAAAGAGGCCGAGGGGAAATTTAAAGAGATCAAGGAAGCTTGTGAAGTTCTGACCGAGTCGGaaaaa                                                                                     >  2:1853223/1‑140 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGa                                                                                                                    >  1:3730483/1‑109 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGa                                                                                                                    <  2:3730483/109‑1 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                     >  1:795548/1‑140 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                     >  1:3825121/1‑140 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAAGACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGGAGTTCTGACCGACTCGCaaaaa                                                                                     >  2:3108362/1‑140 (MQ=255)

                                                  agagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                 <  1:428349/140‑1 (MQ=255)

                                                  agagCGTGAAATCAGAAAGGCCTACAAACGCCTCGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAACGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                 <  2:3497343/140‑1 (MQ=255)

                                                   gcgCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACTACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                <  2:691183/138‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                <  2:4150472/140‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                <  1:1759700/140‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                                <  2:3871756/140‑1 (MQ=255)

                                                   gagCATGAAATCAGAAAGTCCGAAAAACGCCTAGCAATGAAATACAACCCGGACCGTACCCAGGGTGACAAAGAGGGCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTACTGACCGAATCGCAAAAACGTGc                                                                                <  2:2249495/140‑1 (MQ=255)

                                                     gCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                            >  1:13321/1‑110 (MQ=255)

                                                     gCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                            <  2:13321/110‑1 (MQ=255)

                                                       gTGAAATCCGAGAGGTCTAAAAACGCCTGGCCAAAAACTACCACCCCGACCGTAACCAGGGTGAAATAGACGCCAACGCGAAATTTAAACAGACCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                                            <  2:1363058/140‑1 (MQ=255)

                                                       gTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                                            <  1:611755/140‑1 (MQ=255)

                                                       gTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                                            <  1:369378/140‑1 (MQ=255)

                                                        tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                           <  1:3583885/140‑1 (MQ=255)

                                                        tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                           <  2:2703504/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATCCCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGCAATTTACCGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  1:2377374/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  1:4491431/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  1:298392/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  2:3531640/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  1:4974667/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  1:5114542/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATAAAATACCGCCCGGACCGTAACCAGGGTGACAAAGAGTCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  1:3405617/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCATGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                        <  1:10121/140‑1 (MQ=255)

                                                             tCATAAAGGCCCACAAACGCCTGGCCATGAAATACCACCCGGAGCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATCTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                      <  2:3213887/140‑1 (MQ=255)

                                                             tCAAAAAGGCCTACAAACGCCTGGCCATCAACTACCACCCGGCCCGTAACCAGGGTGACAAACAGGCCGAGGCGAAATTTAAACAGATCAAGGAAGCTTATGAAGTTCTGACCGACCCGCAAAAACGTGCGGCATACGAt                                                                      <  2:4785823/140‑1 (MQ=255)

                                                             ccAGAGAGGCCTACAAACGCCTGGTCATGAGCTGCCACCCAGGCGGTTACCAGGGTGACAAAGGGCCCGAAGCGAAATTTAAAGTTATCAACGAAGCTTATGAAGTTCTGACCGTCTCGCAAAAAGGTGCGGCATACGAt                                                                      <  2:2869850/139‑1 (MQ=255)

                                                                gAAAGGCCTACAAACGCCTGGCCATGAAATACCCCCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAg                                                                   <  1:5217012/140‑1 (MQ=255)

                                                                  aaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                 <  1:2901273/140‑1 (MQ=255)

                                                                    ggCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATg                                                               <  1:5576574/140‑1 (MQ=255)

                                                                      ccTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGt                                                             <  1:5051754/140‑1 (MQ=255)

                                                                         aCAAACGCCTGGCCATGAAATACCCCCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGATATCAAGGAAGCTTATGAAGTTCTGACCGCCTCGCAAAAACGTGCGGCATACGATCAGTATGGGCag                                                          >  2:577372/1‑139 (MQ=255)

                                                                         aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAt                                                          >  1:2384663/1‑140 (MQ=255)

                                                                         aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAt                                                          >  1:2621394/1‑140 (MQ=255)

                                                                          cAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtg                                                         >  1:3156838/1‑140 (MQ=255)

                                                                           aaaCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgc                                                        <  1:3781603/140‑1 (MQ=255)

                                                                               gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                    >  1:80593/1‑140 (MQ=255)

                                                                               gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                    >  1:139055/1‑140 (MQ=255)

                                                                               gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                    >  2:875330/1‑140 (MQ=255)

                                                                               gCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCg                                                    >  2:5241141/1‑140 (MQ=255)

                                                                                ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                   <  1:1588772/140‑1 (MQ=255)

                                                                                ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                   >  1:2079686/1‑140 (MQ=255)

                                                                                ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                   <  2:1499787/140‑1 (MQ=255)

                                                                                ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                   <  1:1885082/140‑1 (MQ=255)

                                                                                ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAAGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                   >  1:4787342/1‑140 (MQ=255)

                                                                                  tGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGttt                                                 >  1:2727072/1‑140 (MQ=255)

                                                                                    gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                                               >  1:1669720/1‑140 (MQ=255)

                                                                                    gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                                               >  2:2256315/1‑140 (MQ=255)

                                                                                    gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGAGCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTGTGa                                               >  1:2171092/1‑140 (MQ=255)

                                                                                    gCCATGAAATACCACCCGGACCGTAACCAGGGTGAAAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCGTATGGAGTTCTGACCGAGTGGCAAAAACGGGGGGCATACGATCAGTATGGTGATGCTGCGGTTgg                                               >  2:941008/1‑139 (MQ=255)

                                                                                     ccATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGTATACGATCAGTATGGTCATGCTGCGTTTGAg                                              <  1:5280631/140‑1 (MQ=255)

                                                                                     ccATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAg                                              <  1:1990231/140‑1 (MQ=255)

                                                                                         gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                          >  1:4618669/1‑140 (MQ=255)

                                                                                              accaccCGGACCGTAACCAGGGTGACAAAGAGGGCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATAGGAGCCGTATGGTCATGCTGCGTGTGAGGAAGGTGGc                                     >  2:3000511/1‑140 (MQ=255)

                                                                                              accaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGc                                     >  1:1811628/1‑140 (MQ=255)

                                                                                                  cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                 >  1:1067964/1‑140 (MQ=255)

                                                                                                  cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                 >  1:5248563/1‑140 (MQ=255)

                                                                                                   ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATggg                                >  2:597120/1‑140 (MQ=255)

                                                                                                    cGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATggtg                               >  2:4712842/1‑138 (MQ=255)

                                                                                                       aCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggc                            >  1:875165/1‑140 (MQ=255)

                                                                                                       aCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTGACAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTACGGTCGTGCTGCGTTTGAGCAAGGTGGCATGggcggg                            >  1:4402877/1‑139 (MQ=255)

                                                                                                          gTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                         >  1:2199834/1‑140 (MQ=255)

                                                                                                          gTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                         >  2:4815964/1‑140 (MQ=255)

                                                                                                          gTAACAAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTTCGGCATACGATCAGTATGGTCATGCCGCGTGTGAGCAAGGTGGCATGggcggcggc                         >  2:5091585/1‑140 (MQ=255)

                                                                                                             aCCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGt                      >  2:4368101/1‑140 (MQ=255)

                                                                                                                 gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTCTGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGcggcggcggggtgg                  >  2:5545519/1‑135 (MQ=255)

                                                                                                                     gACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg              <  2:4286816/140‑1 (MQ=255)

                                                                                                                     gACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg              >  1:3283914/1‑140 (MQ=255)

                                                                                                                     gACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg              <  1:1237165/140‑1 (MQ=255)

                                                                                                                     gACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcgg              <  1:1416890/140‑1 (MQ=255)

                                                                                                                      aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc             >  1:117596/1‑140 (MQ=255)

                                                                                                                      aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc             >  2:4880550/1‑140 (MQ=255)

                                                                                                                      aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc             >  2:3401707/1‑140 (MQ=255)

                                                                                                                      aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc             >  1:1976798/1‑140 (MQ=255)

                                                                                                                      aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc             >  1:3816007/1‑140 (MQ=255)

                                                                                                                            atgCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACTGACTCGCAAACACGTGCGGCATACGATCAGTATGTGCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCTGTTGTGGGAGCGgcgcg       >  2:2079051/3‑139 (MQ=255)

                                                                                                                            agGCCGAGGCCAAATTTAAAGAGATCAAGGAAGCTTATGACGTTCTGACCGTCTCGCAAAAACGTGCGGCATACGATCAGTATGTTCCTGCTTCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa       <  2:1294387/140‑1 (MQ=255)

                                                                                                                             ggCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAg      >  2:5306336/1‑140 (MQ=255)

                                                                                                                             ggCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAg      >  1:5325568/1‑140 (MQ=255)

                                                                                                                                cGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACt   >  2:1228298/1‑140 (MQ=255)

                                                                                                                                cGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACt   >  1:3185268/1‑140 (MQ=255)

ATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTT  >  NZ_CP025268/14169‑14437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`