BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,738,522	A→G	1539 (TAA→TAG)	lhr →	ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,738,522	0	A	G	99.6%	900.4 / NA	273	1539 (TAA→TAG)	lhr	ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (140/132); minor base T (0/1); total (140/133)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 285 total aligned reads displayed.

CCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTCG  >  NZ_CP025268/1738386‑1738657

ccGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATagaga                                                                                                                                      <  1:2677606/140‑1 (MQ=255)

 cGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATagagaa                                                                                                                                     <  1:1616775/140‑1 (MQ=255)

  gCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGCTCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATagagaaa                                                                                                                                    >  1:3058753/1‑140 (MQ=255)

  gCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATagagaaa                                                                                                                                    >  1:2459022/1‑140 (MQ=255)

      tGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGAGAGAGAAAGGAc                                                                                                                                >  2:3288373/1‑140 (MQ=255)

      ggCGTCGCGAAACTCGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGAc                                                                                                                                <  2:4847731/139‑1 (MQ=255)

        cGTCGCGCACCACGGCTGCGCTTTACGCCCACAGAAGTGCATGATCTACCCGTCCGGCACACGCCGATGTTTACGCTGCTGCGTGAGGCGGGCTTTTCAAGTTCGCCACAAGTGTTGGAGGGGGGGTAGAGGAAGGACTg                                                                                                                              >  2:519768/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  2:645099/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  1:4760789/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  2:3867370/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  1:3217626/1‑140 (MQ=255)

        cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGGTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTg                                                                                                                              >  2:1512680/1‑140 (MQ=255)

        cGTCCCGCACTACGGATGGGCTTTACGCTAACCGAAGTGAATGATCTACCGGTCCGGCAAACGGCGATGTTTACGCTGCTGCGTGAGGCGGGCTTTTCAAGTTCGCCACAAGGTCTGGATTGGGGATAGAGCGAGGACTg                                                                                                                              >  2:1767778/1‑140 (MQ=255)

           cgcgAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACg                                                                                                                           <  1:3700379/140‑1 (MQ=255)

             cgAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGa                                                                                                                         >  2:3812269/1‑140 (MQ=255)

                aCCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGc                                                                                                                      <  1:362348/140‑1 (MQ=255)

                  cACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACGGACGGATGccc                                                                                                                    >  2:3074894/1‑140 (MQ=255)

                  cACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGAGGGGGGGGAGAGAAAGGACTGACGGATGccc                                                                                                                    >  1:2014278/1‑140 (MQ=255)

                     ggCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGtt                                                                                                                 >  2:2266696/1‑140 (MQ=255)

                         gcgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                             <  2:4232010/140‑1 (MQ=255)

                          cgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGAGGTTTACGCTGCTGCGTGAGTCGGGATGTTCAAGTGCGCCACAATGGGTGGATTGGGGATAGAGATAGGACTGACGGATGTCCGTTCGCAt                                                                                                            >  2:696655/1‑140 (MQ=255)

                              ttACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGt                                                                                                        <  2:3615781/140‑1 (MQ=255)

                              ttACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGt                                                                                                        <  1:3369298/140‑1 (MQ=255)

                              tgaCGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGt                                                                                                        <  2:1669558/138‑1 (MQ=255)

                               tACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTc                                                                                                       <  2:4170268/140‑1 (MQ=255)

                                 cGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                                     >  2:3363471/1‑140 (MQ=255)

                                 cGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAg                                                                                                     <  1:2856038/140‑1 (MQ=255)

                                      aCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTATGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                                                <  2:3215455/140‑1 (MQ=255)

                                      aCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                                                <  1:433110/140‑1 (MQ=255)

                                      aCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                                                >  1:402727/1‑140 (MQ=255)

                                      aCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                                                >  1:2132870/1‑140 (MQ=255)

                                      aCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGAGTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                                                >  2:4425135/1‑140 (MQ=255)

                                       cAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGc                                                                                               >  1:1063418/1‑140 (MQ=255)

                                        aGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCa                                                                                              <  2:4340845/140‑1 (MQ=255)

                                         gAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAg                                                                                             >  1:5076047/1‑140 (MQ=255)

                                          aaGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  1:233768/140‑1 (MQ=255)

                                          aaGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  2:2591821/140‑1 (MQ=255)

                                          aaGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTCCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGTGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAgg                                                                                            <  1:3344352/140‑1 (MQ=255)

                                            gTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGAc                                                                                          >  2:3091389/1‑140 (MQ=255)

                                              gAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACgg                                                                                        >  2:2093806/1‑140 (MQ=255)

                                                aTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCa                                                                                                             >  1:4547638/1‑117 (MQ=255)

                                                aTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGat                                                                                      >  1:1277355/1‑140 (MQ=255)

                                                aTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGat                                                                                      >  1:4260120/1‑140 (MQ=255)

                                                aTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGat                                                                                      >  1:4849049/1‑140 (MQ=255)

                                                    tCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGattatt                                                                                  >  1:1737111/1‑140 (MQ=255)

                                                    tCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGattatt                                                                                  >  1:4574180/1‑140 (MQ=255)

                                                    tCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGattatt                                                                                  >  1:4215302/1‑140 (MQ=255)

                                                       aCCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcg                                                                               >  2:5385329/1‑140 (MQ=255)

                                                           gttcGGCAAACGCCTAGGTTTCCGCCGCGGCGTGAGGCGGGATTTTCAAGTTCGCCACAAAGGCTGGATTGGGGATAGAGAAGGGACTGAGGGGTGCCGGTTCGCATCCGTTATGATTACAGGAGGGATCATCCGGCGTc                                                                           >  2:1917371/4‑140 (MQ=255)

                                                           gTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTc                                                                           <  2:3728780/140‑1 (MQ=255)

                                                           gTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTc                                                                           <  2:3882948/140‑1 (MQ=255)

                                                           gTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTc                                                                           >  1:3451448/1‑140 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTGGCCACAAGGGTTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTTCgg                                                                        <  1:254887/140‑1 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        <  2:47546/140‑1 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        >  2:547447/1‑140 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        <  2:1182668/140‑1 (MQ=255)

                                                              cGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCgg                                                                        >  1:873070/1‑140 (MQ=255)

                                                                gCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCCAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGct                                                                      >  1:3261305/1‑140 (MQ=255)

                                                                gCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGct                                                                      >  1:620499/1‑140 (MQ=255)

                                                                   aaCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCtt                                                                   <  2:1471051/140‑1 (MQ=255)

                                                                   aaCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCtt                                                                   <  1:1896793/140‑1 (MQ=255)

                                                                     cGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGTATTGGGGATAGAGAAAGGACTGACGGATGCCCTTTCGCATCTGTCAGTATTGCAGGGCGGATTATTCCGCGTCCGGCTCTTCa                                                                 <  2:2384735/140‑1 (MQ=255)

                                                                     cGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                                 <  1:4970714/140‑1 (MQ=255)

                                                                     cGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                                 <  1:4126570/140‑1 (MQ=255)

                                                                     cGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTAGGGATAGAGAAAGGACTGCCGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                                 <  1:1199391/140‑1 (MQ=255)

                                                                      gCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCAGGACTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAg                                                                >  2:595924/1‑140 (MQ=255)

                                                                          aTGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACtt                                                            >  2:2913338/1‑140 (MQ=255)

                                                                          aTGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACtt                                                            >  1:4917201/1‑140 (MQ=255)

                                                                                     gctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCgg                                                 >  2:2542991/1‑140 (MQ=255)

                                                                                     gctgcGGGAGGTGGGAGTTTCAAGTTCGCCACAAGGCGTAGATGGGGTATAGAGAAAAGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGCCCGCCCCTTCAGAATGGGATTCAGCgg                                                 >  2:890671/1‑140 (MQ=255)

                                                                                      ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                                >  2:5512275/1‑140 (MQ=255)

                                                                                      ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                                >  2:2471460/1‑140 (MQ=255)

                                                                                      ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                                >  2:2336802/1‑140 (MQ=255)

                                                                                      ctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                                                >  2:4438799/1‑140 (MQ=255)

                                                                                             aGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCt                                         <  2:4619969/140‑1 (MQ=255)

                                                                                              ggCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCtt                                        >  1:729060/1‑140 (MQ=255)

                                                                                              ggCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGGTTCtt                                        >  2:4911898/1‑140 (MQ=255)

                                                                                                cGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTg                                      >  1:2968226/1‑140 (MQ=255)

                                                                                                   gagtttCCAGTTCGCCACACGGGCTGGATTGGGGATAGAGACAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATc                                   >  2:3927650/4‑140 (MQ=255)

                                                                                                   gATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATc                                   >  1:2807751/1‑140 (MQ=255)

                                                                                                      tttCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAgc                                <  2:2971865/140‑1 (MQ=255)

                                                                                                      tttCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAgc                                <  2:2694617/140‑1 (MQ=255)

                                                                                                           aGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGctgctg                           <  2:5243782/140‑1 (MQ=255)

                                                                                                           aGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGctgctg                           <  1:400890/140‑1 (MQ=255)

                                                                                                             ttCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCa                         <  1:1367481/140‑1 (MQ=255)

                                                                                                             ttCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCAGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCa                         <  1:4657988/140‑1 (MQ=255)

                                                                                                               cGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGt                       >  1:1948373/1‑140 (MQ=255)

                                                                                                                  cacaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCg                    <  2:5091759/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  1:5256190/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  1:385368/140‑1 (MQ=255)

                                                                                                                   acaAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGc                   <  1:3535479/140‑1 (MQ=255)

                                                                                                                     aaGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATGCGTCAGTATTGCAGGGCGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGGGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCa                 <  2:4065067/140‑1 (MQ=255)

                                                                                                                       gggCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACg               <  1:1528589/140‑1 (MQ=255)

                                                                                                                          cTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTg            >  2:3670469/1‑140 (MQ=255)

                                                                                                                           tGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGa           <  2:3508899/140‑1 (MQ=255)

                                                                                                                            ggATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                                 >  1:3753398/1‑85 (MQ=255)

                                                                                                                            ggATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCa                                                                 <  2:3753398/85‑1 (MQ=255)

                                                                                                                            ggATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGAt          >  1:5482150/1‑140 (MQ=255)

                                                                                                                                   ggATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTc   >  1:181884/1‑140 (MQ=255)

                                                                                                                                    gATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTCg  <  2:1397841/140‑1 (MQ=255)

CCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAAAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTCGCCACGCTGATACATTTCG  >  NZ_CP025268/1738386‑1738657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`