Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,766,333 A→G C92R (TGT→CGT)  sufD ← Fe‑S cluster assembly protein SufD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,766,3330AG99.8% 1331.2 / NA 401C92R (TGT→CGT) sufDFe‑S cluster assembly protein SufD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (187/212);  minor base T (0/1);  total (187/214)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

CACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTC  >  NZ_CP025268/1766196‑1766468
                                                                                                                                         |                                                                                                                                       
cACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGCCGGTCGTCGTTAATGCTCCCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGcc                                                                                                                                       >  1:4003905/1‑140 (MQ=255)
  cGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCa                                                                                                                                     >  1:5391262/1‑140 (MQ=255)
       tgtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGAc                                                                                                                                >  1:3409729/1‑140 (MQ=255)
       tgtgCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGAc                                                                                                                                >  2:4535173/1‑140 (MQ=255)
       tgtaCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTGGGAAAAACCCTGACGGTCGACGTTAATGCTCAATTCATATCCGCTGCCTTCAGTATCATTGCTCAGTGTGGGCACGTAACGCCCATCgcc                                                                                                                                >  2:4199155/1‑138 (MQ=255)
              ggCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGATCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACAcc                                                                                                                         >  2:5163225/1‑140 (MQ=255)
               gCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCa                                                                                                                        >  2:109636/1‑140 (MQ=255)
                cTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAg                                                                                                                       <  2:5576745/140‑1 (MQ=255)
                 tttCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGc                                                                                                                      <  2:3623602/140‑1 (MQ=255)
                   tCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCg                                                                                                                    >  2:1961147/1‑140 (MQ=255)
                     cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCCCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                  <  1:585014/140‑1 (MQ=255)
                     cGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                  <  1:5233370/140‑1 (MQ=255)
                      gTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCAc                                                                                                                 <  1:2020965/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCCCGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:5283752/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:5318416/140‑1 (MQ=255)
                       tCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  2:55056/140‑1 (MQ=255)
                       tCAAATGCAAAAACACTTCCGCCTGCATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACg                                                                                                                <  1:3318441/140‑1 (MQ=255)
                        cAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACgg                                                                                                               <  1:1004221/140‑1 (MQ=255)
                             gCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCt                                                                                                          <  1:4174409/140‑1 (MQ=255)
                                 aaaCACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACg                                                                                                      >  2:622562/1‑140 (MQ=255)
                                   acccTTCCGCCTGAATAGCGTCGGGTAAGCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                    <  2:2267726/137‑1 (MQ=255)
                                   acacTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGtt                                                                                                    <  2:5059528/140‑1 (MQ=255)
                                     acTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTaa                                                                                                  <  1:4609006/140‑1 (MQ=255)
                                       ttCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACg                                                                                                >  2:703972/1‑140 (MQ=255)
                                         ccGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCt                                                                                              >  1:2463101/1‑140 (MQ=255)
                                            ccTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAg                                                                                           >  1:435986/1‑140 (MQ=255)
                                             cTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAgg                                                                                          <  2:4887815/140‑1 (MQ=255)
                                               gAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCa                                                                                        >  2:1194803/1‑140 (MQ=255)
                                                aaTAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCAt                                                                                       <  2:4468217/140‑1 (MQ=255)
                                                 aTAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCCCGGAGTCTAACGTTAACGCTAAGGCATc                                                                                      >  2:3863868/1‑140 (MQ=255)
                                                 aTAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATc                                                                                      >  2:2589139/1‑140 (MQ=255)
                                                  tAGCGTCGGGTGAACCCTGACGGTCGTAGTCAATGCTCACTTCACATCCGCTGCATTCAGTTGCATCGCTCAGTGCGGGCACGTAACCCCCATTGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCa                                                                                     <  2:1663732/140‑1 (MQ=255)
                                                  tAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCa                                                                                     <  1:2964492/140‑1 (MQ=255)
                                                    gCGTCGGGTCAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  1:1574610/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGCCAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:1310974/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:5392664/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:5344824/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  2:880653/140‑1 (MQ=255)
                                                    gCGTCGGGTAAACCCTGACGGTCGTCGTCAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCTCACGGAGTCTAACGTTAACGCTAAGGCATCACg                                                                                   <  1:5539251/140‑1 (MQ=255)
                                                     cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGccc                                                                                                                                      <  1:3237411/88‑1 (MQ=255)
                                                     cGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgc                                                                                  >  2:1413515/1‑140 (MQ=255)
                                                      gTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgct                                                                                 <  1:2645304/140‑1 (MQ=255)
                                                       tCGGGTAAGCCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                                                <  1:1176285/140‑1 (MQ=255)
                                                       tCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGTGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                                                >  1:4913064/1‑140 (MQ=255)
                                                       tCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                                                <  2:4835204/140‑1 (MQ=255)
                                                       tCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACgctg                                                                                >  2:2355783/1‑140 (MQ=255)
                                                          ggTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGctgctg                                                                             <  2:2724592/140‑1 (MQ=255)
                                                                 ccTGCCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGTACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGat                                                                      <  2:3158912/140‑1 (MQ=255)
                                                                 ccTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGat                                                                      <  2:3409729/140‑1 (MQ=255)
                                                                    gACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtc                                                                   >  2:4137694/1‑140 (MQ=255)
                                                                     aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGTCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGGCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGCTAtct                                                                  >  2:4798006/1‑140 (MQ=255)
                                                                     aCGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATAtct                                                                  >  1:812382/1‑140 (MQ=255)
                                                                          cgtcgtTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTccc                                                             >  2:853845/1‑140 (MQ=255)
                                                                            tgggtAATGCTCACTTCATGTCCGGTGGCTTCAGTTGGATCGGTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGGGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCCCTCCCGc                                                           <  2:3496517/136‑1 (MQ=255)
                                                                                  aTGCTCACTTCATATCCGATGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGTAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCGCCCGCAATGCt                                                     <  2:3985075/140‑1 (MQ=255)
                                                                                     cTCACTTCATATCCGCTGCCTTCAGTTGCGTCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGAc                                                  <  2:3012594/140‑1 (MQ=255)
                                                                                     cTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGAc                                                  >  1:3798880/1‑140 (MQ=255)
                                                                                       cACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaa                                                >  1:1065121/1‑140 (MQ=255)
                                                                                        aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                               >  1:4886557/1‑140 (MQ=255)
                                                                                        aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                               <  1:632323/140‑1 (MQ=255)
                                                                                        aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACaaa                                               <  1:5148301/140‑1 (MQ=255)
                                                                                        aCTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGAACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTGTCCCGCAATGCTGACaaa                                               <  2:2847626/140‑1 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             <  1:4053031/140‑1 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             <  1:4504017/140‑1 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             >  2:498402/1‑140 (MQ=255)
                                                                                          ttCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACt                                             <  1:2126016/140‑1 (MQ=255)
                                                                                               atCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTa                                        <  2:822932/140‑1 (MQ=255)
                                                                                               atCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGCAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTa                                        <  1:5372838/140‑1 (MQ=255)
                                                                                                  cGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGCCAAACTGGCTATTg                                     >  2:2544212/1‑140 (MQ=255)
                                                                                                  cGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGATAACGCTCAGACATCACGCTGCTGTGCGGATATCTCTCCCGCAATTCTGACAAACTGGCTAtgg                                     >  2:5312111/1‑138 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATGGACAAACACCAGCGGCACGGGGTCTAACGTTAACGCTAAGGCATTGCGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACGGGCTATTGag                                   >  2:2076495/1‑139 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCa                                                                                                                  <  1:3623162/61‑1 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCGCGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:1317470/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAACCTGGCTATTGAt                                   >  1:4961665/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:942835/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:1837759/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  2:3698042/1‑140 (MQ=255)
                                                                                                    cTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGAt                                   >  1:3739127/1‑140 (MQ=255)
                                                                                                      gCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCa                                 >  2:4715564/1‑140 (MQ=255)
                                                                                                      gCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCa                                 <  2:2876784/140‑1 (MQ=255)
                                                                                                       ccTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAg                                >  1:4755406/1‑140 (MQ=255)
                                                                                                        cTTCAGTTGCATCGCTCAGTGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGc                               <  1:2833114/140‑1 (MQ=255)
                                                                                                               tGCATCGCTCAGTGCGGGAACGTAACGCCCATCGACAACCACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCa                        <  1:3009503/140‑1 (MQ=255)
                                                                                                                           tGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATAtt            >  1:3485213/1‑140 (MQ=255)
                                                                                                                           tGCGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATAtt            >  1:150979/1‑140 (MQ=255)
                                                                                                                             cGGGCACGTCACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTc          <  2:1792276/140‑1 (MQ=255)
                                                                                                                             cGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTc          <  2:3729847/140‑1 (MQ=255)
                                                                                                                             cGGGCACGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTc          <  1:5108386/140‑1 (MQ=255)
                                                                                                                                   cGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGttt    >  1:3885910/1‑140 (MQ=255)
                                                                                                                                   cGTAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGCATATTTCCAGttt    >  2:2820456/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCGTCGACAAACACCAGCCGCACGGAGTCTACCGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCTTATATTTCCAGTTTTc  >  2:1548196/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAgcgg                    <  2:1051312/122‑1 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:2273222/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:2395100/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  <  1:2589139/140‑1 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  1:698519/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTc  >  2:2437189/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGACGTATATTTCCAGTTTTc  >  2:1353042/1‑140 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCAGAGGGGATATCTCTCCCGCAATGCTGACAAACGGGCTATTCATCAGCCCTTCCAGCGGCGGACATTTAAAGttttg  >  2:4359174/1‑139 (MQ=255)
                                                                                                                                     tAACGCCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACCCTGCTGTGGGGATATCCCTCCCGCAATGCCGACAAACTGGCTATGAATCAGCCCTTCCAGCGGCACATCCTGCCAGTTTTc  >  2:3216731/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
CACGCTTTGTGCCAGGCTTTCCGTCAAATGCAGAAACACTTCCGCCTGAATAGCGTCGGGTAAACCCTGACGGTCGTCGTTAATGCTCACTTCATATCCGCTGCCTTCAGTTGCATCGCTCAGTGCGGGCACGTAACACCCATCGACAAACACCAGCCGCACGGAGTCTAACGTTAACGCTAAGGCATCACGCTGCTGTGGGGATATCTCTCCCGCAATGCTGACAAACTGGCTATTGATCAGCCCTTCCAGCGGCGTATATTTCCAGTTTTC  >  NZ_CP025268/1766196‑1766468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑