BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,808,384	A→G	pseudogene (474/1900 nt)	espL1 →	type III secretion system effector EspL1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,808,384	0	A	G	99.6%	914.8 / NA	275	pseudogene (474/1900 nt)	espL1	type III secretion system effector EspL1
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (186/88); total (187/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

TAACCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAATGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATAC  >  NZ_CP025268/1808249‑1808518

 aaCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCAt                                                                                                                                   >  2:2719793/1‑140 (MQ=255)

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  1:5295166/1‑140 (MQ=255)

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  1:3647718/1‑140 (MQ=255)

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  1:4236859/1‑140 (MQ=255)

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  2:4278657/1‑140 (MQ=255)

  aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                  >  1:129742/1‑140 (MQ=255)

            aaGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCaa                                                                                                                        >  2:1011655/1‑140 (MQ=255)

            aaGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCaa                                                                                                                        >  1:2071680/1‑140 (MQ=255)

                    tGGATGATATCGAACAGGAACAATTAACTGCTGACGAGCGTAACCATTGTACGAACCTCGCTACAAAAAGCCATAGAGACAAAACCTATACCCCCAACAGTAAACCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTc                                                                                                                <  2:3389040/140‑1 (MQ=255)

                      gATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCctgctg                                                                                                              <  1:2736051/140‑1 (MQ=255)

                       aTTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGa                                                                                                             >  2:3854785/1‑140 (MQ=255)

                       aTTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGa                                                                                                             >  1:1794220/1‑140 (MQ=255)

                         tataTTGAACAGGAACGGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACg                                                                                                           >  1:3418997/1‑140 (MQ=255)

                         tataTTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACg                                                                                                           >  2:4864850/1‑140 (MQ=255)

                         tataTTGAACAGCAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACg                                                                                                           >  2:5531043/1‑140 (MQ=255)

                           tatTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCt                                                                                                         >  2:102729/1‑140 (MQ=255)

                           tatTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAATGCATGTTTTTTGCAATCTCCTTCTGACGCt                                                                                                         >  2:4452899/1‑140 (MQ=255)

                              tGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtt                                                                                                      >  1:4558133/1‑140 (MQ=255)

                              tGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtt                                                                                                      >  1:747306/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGCTCGTAACCATTGTATGAAACTGTCTAAAAAACTCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  2:1351362/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGCAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGTAATCTTCTTCTGACGCTAtttt                                                                                                    >  1:3107033/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  1:2297660/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  1:4194540/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  1:5126089/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  1:3778727/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                    >  2:3553315/1‑140 (MQ=255)

                                aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGGGCATGTTTTTTACAATCTCCTTCTGACGCTAgttt                                                                                                    >  2:848403/1‑140 (MQ=255)

                                aacaggaacagTTAACAGCTGAAGATCGTAACCATTGTATGAAACTGTGTAAAAAAATCAATAGAGAAAAAAACTATACACCTAACAGTAAGTCTCAATGGTAGTGCATGTTTTTTGCAATATGATTCTGACGCTAtgtt                                                                                                    >  2:3573693/1‑140 (MQ=255)

                                     gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                               >  2:628070/1‑140 (MQ=255)

                                        cagTTAACTGCTTAAGATCGTAACCATTATATGATACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACTGTACATCTCAATGCTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGcc                                                                                            <  2:4384517/140‑1 (MQ=255)

                                              aCTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGt                                                                                      <  2:2196698/140‑1 (MQ=255)

                                               ctgctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTc                                                                                     >  2:470278/1‑140 (MQ=255)

                                                tgctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCa                                                                                    >  2:2733850/1‑140 (MQ=255)

                                                         tCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATAc                                                                           <  1:2501586/140‑1 (MQ=255)

                                                         tCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATAc                                                                           <  2:802464/140‑1 (MQ=255)

                                                          cGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAACAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACg                                                                          >  1:3812621/1‑140 (MQ=255)

                                                          cGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACg                                                                          >  1:2580007/1‑140 (MQ=255)

                                                            tAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGcc                                                                        >  1:3027514/1‑140 (MQ=255)

                                                            tAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGcc                                                                        >  1:270507/1‑140 (MQ=255)

                                                            tAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGcc                                                                        >  1:2395900/1‑140 (MQ=255)

                                                            tAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGcc                                                                        >  1:875688/1‑140 (MQ=255)

                                                             aaCCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATATGccc                                                                       <  2:5137503/140‑1 (MQ=255)

                                                             aaCCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGccc                                                                       <  1:1012181/140‑1 (MQ=255)

                                                                  ttGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAt                                                                  >  1:4825539/1‑140 (MQ=255)

                                                                  ttGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGATATTTTTTGTCGCCATCTGTCATTGAAATACGCCCTTGAt                                                                  >  2:2514989/1‑140 (MQ=255)

                                                                   tGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATATGCCCTTGAtt                                                                 >  1:1159509/1‑140 (MQ=255)

                                                                          aaCTGTCTAAAAAAATCGATACGGAAATAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATCTCTCTTGCAATCTCCTCCTGACGCTCTTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTgag                                                          >  1:4391700/1‑140 (MQ=255)

                                                                          aaCTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTgag                                                          >  2:4148299/1‑140 (MQ=255)

                                                                          aaCTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTgag                                                          >  1:1868148/1‑140 (MQ=255)

                                                                             tGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGaaa                                                       >  1:805167/1‑140 (MQ=255)

                                                                             tGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGaaa                                                       >  1:3737761/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCACTGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:2490662/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTTGCCATCTGTCATTGCAATACTCCCTTGATTCATTGAGAAATg                                                     >  2:4510694/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:2246324/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  2:435736/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  2:3870321/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:3197810/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  2:3062527/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  2:4576430/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:5490645/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:1387144/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:5217530/1‑140 (MQ=255)

                                                                               tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                     >  1:698166/1‑140 (MQ=255)

                                                                                  aaaaaaaTCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGaa                                                  <  2:2815243/140‑1 (MQ=255)

                                                                                      aaaTCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAgg                                              <  2:4769562/140‑1 (MQ=255)

                                                                                         tCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCaa                                           >  2:5448782/1‑140 (MQ=255)

                                                                                           cATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAg                                         >  2:662642/1‑140 (MQ=255)

                                                                                             tAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTATTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTc                                       <  1:2222671/140‑1 (MQ=255)

                                                                                                       aaCTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTa                             <  2:2657210/140‑1 (MQ=255)

                                                                                                       aaCTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTa                             <  1:2796018/140‑1 (MQ=255)

                                                                                                       aaCTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTCGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTa                             <  1:818329/140‑1 (MQ=255)

                                                                                                         cTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTaaa                           <  2:1508583/140‑1 (MQ=255)

                                                                                                            taCACCCCACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACAt                        <  2:3688259/140‑1 (MQ=255)

                                                                                                            taCACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACAt                        <  2:1841798/140‑1 (MQ=255)

                                                                                                                 ccAACAGTAAATCGCAAGGGGAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTATACATTActt                   >  2:417421/1‑139 (MQ=255)

                                                                                                                   aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                 <  2:766110/140‑1 (MQ=255)

                                                                                                                   aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                 >  2:3237613/1‑140 (MQ=255)

                                                                                                                   aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                 <  2:1259179/140‑1 (MQ=255)

                                                                                                                       gTAAATCTCAATGGTCGTGCATGTTTTTCGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGt             <  1:4526404/140‑1 (MQ=255)

                                                                                                                       gTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGt             <  2:88147/140‑1 (MQ=255)

                                                                                                                       gTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGt             <  1:2169534/140‑1 (MQ=255)

                                                                                                                       gTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGt             <  2:3418997/140‑1 (MQ=255)

                                                                                                                       gTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGt             <  1:559953/140‑1 (MQ=255)

                                                                                                                       gTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGt             <  1:470278/140‑1 (MQ=255)

                                                                                                                        tAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGtt            >  1:413486/1‑140 (MQ=255)

                                                                                                                        tAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGtt            >  2:5395820/1‑140 (MQ=255)

                                                                                                                        tAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGtt            >  2:1343561/1‑140 (MQ=255)

                                                                                                                        tAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGtt            >  1:3297855/1‑140 (MQ=255)

                                                                                                                          aaTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGa          >  1:1905064/1‑140 (MQ=255)

                                                                                                                          aaTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGa          <  2:4850063/140‑1 (MQ=255)

                                                                                                                          aaTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGa          <  1:1634606/140‑1 (MQ=255)

                                                                                                                          aaTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGa          <  2:1227043/140‑1 (MQ=255)

                                                                                                                          aaTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCACTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGa          <  1:1094825/140‑1 (MQ=255)

                                                                                                                            tctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAAt        <  2:2320173/140‑1 (MQ=255)

                                                                                                                               cAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCa     <  1:1010151/140‑1 (MQ=255)

                                                                                                                                aaTGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCAt    <  1:4072499/140‑1 (MQ=255)

                                                                                                                                 aTGGTAGTGCATGTTTTTAGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGTTTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATa   <  2:4001147/140‑1 (MQ=255)

                                                                                                                                  tGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAatacatac  >  1:5020526/1‑140 (MQ=255)

                                                                                                                                  tGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATAc  >  1:994887/1‑140 (MQ=255)

                                                                                                                                  tGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTGTGGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAGGGCAAAGTCAACCTGAGTAAACATTACTCTTCCGTTGAATTCATAc  >  2:3194653/1‑140 (MQ=255)

TAACCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAATGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATAC  >  NZ_CP025268/1808249‑1808518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`