BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,957,477	A→G	132 (TAA→TAG)	yecN →	MAPEG family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,957,477	0	A	G	99.3%	911.4 / NA	277	132 (TAA→TAG)	yecN	MAPEG family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (137/138); minor base T (0/2); total (137/140)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

ATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGAT  >  NZ_CP025268/1957340‑1957612

aTGCATTATTACGGTTTTCATCCCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTCCTGGCGACTCTTTGGTATATCCCCTGGGAGTTGTTTTTCTCCCTTCGTTAGCg                                                                                                                                       <  2:4762031/140‑1 (MQ=255)

  gCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGca                                                                                                                                     <  2:4500400/140‑1 (MQ=255)

  gCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGca                                                                                                                                     >  1:4497675/1‑140 (MQ=255)

  gCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGca                                                                                                                                     <  1:2003289/140‑1 (MQ=255)

  gCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCGGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGca                                                                                                                                     <  2:3527821/140‑1 (MQ=255)

       attaCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATa                                                                                                                                <  1:3180991/140‑1 (MQ=255)

         taCGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACg                                                                                                                              <  1:1035501/140‑1 (MQ=255)

          aCGGTTTTCATCACCGTCTGTTCCGCTGGCGGCGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGc                                                                                                                             >  2:4254916/1‑140 (MQ=255)

             gTTTTCATCACCGTCTGTTACGCTGGCGACGATACGGCATGAGCTACACCTGGTGTGCGCTGTTACTGAAAGTACTTGCGAATGTTAGGTATATGCTCTCTGAGTTGGTTATCTCTCTGCGTTAGCGCACAATACGCCAc                                                                                                                          >  2:1546078/1‑140 (MQ=255)

              ttttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAct                                                                                                                         >  2:881741/1‑140 (MQ=255)

              ttttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCAct                                                                                                                         >  1:804084/1‑140 (MQ=255)

                        cGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGGCTCCTGGTGTGCGCTGTTGCTGATGGTGCTGTCGATTCTTTGGTATATGCGCTGGGAGTTGGTGTTCTCCCTGCGTGCGCGCGCCATAAGCCAGTTTCtttttgc                                                                                                               >  2:4380658/1‑138 (MQ=255)

                        cGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTTGGAGTTGGTTTTCTCTCTGCGTTAGCGCACAATACGCCACTTTCTTTTTcc                                                                                                               >  2:5504766/1‑140 (MQ=255)

                        cGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTcc                                                                                                               <  2:1266215/140‑1 (MQ=255)

                        cGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTcc                                                                                                               <  1:3573236/140‑1 (MQ=255)

                        cGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTcc                                                                                                               >  1:3881550/1‑140 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                             >  2:5236700/1‑140 (MQ=255)

                          tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCg                                                                                                             >  2:4161974/1‑140 (MQ=255)

                                ccGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGAtttt                                                                                                       <  2:4651376/140‑1 (MQ=255)

                                  gCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTa                                                                                                     >  2:3643239/1‑140 (MQ=255)

                                  gCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTa                                                                                                     <  1:4219277/140‑1 (MQ=255)

                                   cTGGCGACGTTCCGGCATGAGCGCCACCTGGTGGGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTAc                                                                                                    >  2:496664/1‑140 (MQ=255)

                                                ggCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTGTCTTTTTCCCGGATTTTTACGTTATGTCTGAcc                                                                                       >  2:3903884/1‑140 (MQ=255)

                                                 gCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACcg                                                                                      <  2:1409777/140‑1 (MQ=255)

                                                 gCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACcg                                                                                      <  1:3438633/140‑1 (MQ=255)

                                                     gAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTATCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                                  <  2:2742570/140‑1 (MQ=255)

                                                     gAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                                  <  1:5475958/140‑1 (MQ=255)

                                                     gAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCCGAGAGTTGGTTTTCTCCCTGCGTTAGCGCACAAGACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                                  <  2:5051011/140‑1 (MQ=255)

                                                     gAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTCCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTCCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGCCTCACCGCGac                                                                                  <  2:4944792/140‑1 (MQ=255)

                                                      aGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCTAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCTTTAGCGCACAGTACGCGACTTTCTTTTTGCCGGATTTTTACGTTATGTCTCACCGCGaca                                                                                 >  2:4475561/1‑140 (MQ=255)

                                                      aGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGTGTTGTTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGTATTTTTACGTTATTTCTCACCGCGaca                                                                                 >  2:4246097/1‑140 (MQ=255)

                                                      aGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGaca                                                                                 <  1:3220716/140‑1 (MQ=255)

                                                      aGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGaca                                                                                 >  1:5438836/1‑140 (MQ=255)

                                                        cgcCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACg                                                                               <  2:2338792/140‑1 (MQ=255)

                                                          ccACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                             <  1:4047017/140‑1 (MQ=255)

                                                          ccACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                             <  1:232184/140‑1 (MQ=255)

                                                          ccACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                             <  1:1399386/140‑1 (MQ=255)

                                                          ccACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCt                                                                             <  1:373027/140‑1 (MQ=255)

                                                                  tgtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                     <  2:2540674/140‑1 (MQ=255)

                                                                  tgtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                     <  1:655211/140‑1 (MQ=255)

                                                                  tgtgCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                     <  1:4559777/140‑1 (MQ=255)

                                                                    tgCGCTGTTGCTGATGTTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTTCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcc                                                                   <  1:3111239/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  2:1217791/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  1:3519027/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  2:4076050/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  2:3160076/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  1:4616097/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  2:2419578/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  1:4616431/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTATTTCCCGGATTTTTACGTTATGTCTCAACGCGACACGCTATTTTCTGcccc                                                                 <  1:3360686/140‑1 (MQ=255)

                                                                      cgcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTATTTTTCCCGGATCTTTACCTTCTGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  2:3716871/140‑1 (MQ=255)

                                                                      cgcTGTTGACGATGGTGCTAGCGAATCTTTGGTATCTGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGAACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGccac                                                                 <  1:43371/140‑3 (MQ=255)

                                                                      cgcTATTGCGGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGCTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                                 <  2:2259140/140‑1 (MQ=255)

                                                                       gcTGTTGCTGATGGTGCTGGCGAATCTTTGGCATATGCCCTGGGAGTTGGTTTTCTCCCTGCGGTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACCCTATTTTCTCCCCCt                                                                >  1:5200696/1‑140 (MQ=255)

                                                                        cTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTa                                                               >  2:3405770/1‑140 (MQ=255)

                                                                           ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTGTCACCGCGACACGCTATGTTCTGCCCCTATct                                                            >  2:4699909/1‑139 (MQ=255)

                                                                           ttGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCg                                                            >  1:2790420/1‑140 (MQ=255)

                                                                               tGATGGGCCTGGCGAGTCTTTAGTATGCGCCCTGGGAGTTCGTTCTCTCCCCGACTTAGCGGACATTACGACCCTTTCTCTGTCACGTAGTGGTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTCTCGCCAg                                                        <  2:1686390/140‑1 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                    >  1:1844407/1‑140 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                    <  1:3420003/140‑1 (MQ=255)

                                                                                   ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTg                                                    <  1:5053660/140‑1 (MQ=255)

                                                                                    gTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTgg                                                   <  1:1807917/140‑1 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                  >  1:2143133/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                  >  2:557016/1‑140 (MQ=255)

                                                                                     tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATGTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTggg                                                  >  1:5219335/1‑140 (MQ=255)

                                                                                       cTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCg                                                >  2:3788356/1‑140 (MQ=255)

                                                                                       cTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTGTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTAATGTCTCACCGCGACACGCTATTTTTTGCCCCTATCGCCAGACTGGGCg                                                >  2:4471007/1‑140 (MQ=255)

                                                                                        tGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGa                                               >  1:599740/1‑140 (MQ=255)

                                                                                         ggCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGAc                                              <  1:5236700/140‑1 (MQ=255)

                                                                                          gCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACt                                             >  1:197293/1‑140 (MQ=255)

                                                                                              aTCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAc                                         <  1:2486603/140‑1 (MQ=255)

                                                                                              aTCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGAc                                         <  1:2366740/140‑1 (MQ=255)

                                                                                               tCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCTACTGGAcc                                        >  2:522557/1‑140 (MQ=255)

                                                                                                    ggTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTg                                                                     <  1:2052305/106‑1 (MQ=255)

                                                                                                     gTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgat                                  <  2:2844443/140‑1 (MQ=255)

                                                                                                       atatGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgatga                                <  2:3272420/140‑1 (MQ=255)

                                                                                                        tatGCCCTGGAGATTGGTTTTCTCCCTGCGTTATCGCACAATACGCCACTTTGTTGTTCTCGGATTTTTACGTTATGTCTCACCGCGACACGCCATTTTCTGCCCCTAGCGGCAGACTGGGCGACTGGACCTTttatgac                               <  2:5175686/140‑2 (MQ=255)

                                                                                                         atGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAAc                              >  2:2684903/1‑140 (MQ=255)

                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACgg                            >  2:5352314/1‑140 (MQ=255)

                                                                                                           gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACgg                            >  1:1494891/1‑140 (MQ=255)

                                                                                                           gCCCTGGGAGTTGG‑TTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                           >  1:4254451/1‑140 (MQ=255)

                                                                                                            cccTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                           <  2:2115089/140‑1 (MQ=255)

                                                                                                            cccTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACggg                           <  2:3562514/140‑1 (MQ=255)

                                                                                                               tGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAg                        >  1:3956381/1‑140 (MQ=255)

                                                                                                               tGGGAGTTGGTTTTCTCCCTCCATTAGCGCACAATACGCCACTTTATTTTTCCCGGATTTTTACGTTATGTCAGATCGCGACACGCTTTTTTCCGCGCCTATCGCCAGCCTGGGCGACTGGACCTTCGATGAACGGGTAg                        >  2:1094527/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                       >  1:1666356/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                       >  2:1961012/1‑140 (MQ=255)

                                                                                                                gggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGc                       >  1:1414773/1‑140 (MQ=255)

                                                                                                                 ggAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCt                      >  2:1756704/1‑140 (MQ=255)

                                                                                                                   aGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGa                    <  2:1854126/140‑1 (MQ=255)

                                                                                                                    gTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGaa                   <  1:4722132/140‑1 (MQ=255)

                                                                                                                     ttGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTGACGATATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTCGCTGagg                  >  2:3345589/1‑138 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGCCCTTTGATGAACGGGTAGCTGAAGTc                >  2:1425476/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                >  2:2142075/1‑140 (MQ=255)

                                                                                                                       ggTTTTCTCCCTACGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTc                >  2:5516468/1‑140 (MQ=255)

                                                                                                                           ttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTcc            >  2:3477445/1‑140 (MQ=255)

                                                                                                                           ttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTcc            <  2:727569/140‑1 (MQ=255)

                                                                                                                           ttCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTcc            <  1:1136172/140‑1 (MQ=255)

                                                                                                                                  tGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCTGatat     >  2:3941913/1‑140 (MQ=255)

                                                                                                                                   gCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATg    <  1:1326776/140‑1 (MQ=255)

ATGCATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGATATGAT  >  NZ_CP025268/1957340‑1957612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`