BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	184,087	T→C	271 (TAA→TAG)	yaeI ←	phosphodiesterase YaeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	184,087	0	T	C	99.6%	803.9 / NA	241	271 (TAA→TAG)	yaeI	phosphodiesterase YaeI
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (95/144); total (96/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

ATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACA  >  NZ_CP025268/183952‑184222

aTTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTaca                                                                                                                                     <  1:1486782/140‑1 (MQ=255)

 ttGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAAACAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTacac                                                                                                                                    >  1:5404874/1‑140 (MQ=255)

   gtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCa                                                                                                                                  >  1:471298/1‑140 (MQ=255)

    tgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAg                                                                                                                                 >  1:4563081/1‑140 (MQ=255)

      cTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGtt                                                                                                                               >  2:2866828/1‑140 (MQ=255)

      cTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGtt                                                                                                                               >  1:3842374/1‑140 (MQ=255)

       ttATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTc                                                                                                                              >  2:1136516/1‑140 (MQ=255)

         aTTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCa                                                                                                                            >  2:3397511/1‑140 (MQ=255)

           tttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGc                                                                                                                          <  1:1252325/140‑1 (MQ=255)

           tttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAACCCTTTTTCTTCCAGACTTCTGTCATGGCGTAAAAATCAAAACATTACCTGCTTTATTCTGGTCATAAAAATCACGATCTACACCAGTTCCAGc                                                                                                                          <  1:3342920/140‑1 (MQ=255)

              aGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATc                                                                                                                       >  2:4141743/1‑140 (MQ=255)

                   ttGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGTTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTAc                                                                                                                  >  1:2935831/1‑140 (MQ=255)

                     ggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAAGCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACtt                                                                                                                >  1:713889/1‑140 (MQ=255)

                           gcccgcccGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggc                                                                                                          >  1:1163973/1‑140 (MQ=255)

                            cccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcg                                                                                                         >  1:4266393/1‑140 (MQ=255)

                             ccgcccgGCTCTACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        <  1:4133097/140‑1 (MQ=255)

                             ccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTTTTTAATGTTACGGCGACCTCCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        <  2:2586446/140‑1 (MQ=255)

                             ccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        <  1:4920351/140‑1 (MQ=255)

                             ccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        <  1:1077815/140‑1 (MQ=255)

                                cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                     >  2:2501700/1‑140 (MQ=255)

                                cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                     >  2:4483864/1‑140 (MQ=255)

                                cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGAGCTACACCAGTTCCAGCATCGTTACTTCCGGGCCGCAg                                                                                                     >  2:5264655/1‑140 (MQ=255)

                                   ggCTCGTCTGTGATCGCAGAAAGCATTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTTTTTATTCAGACGGTGAAATCCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTc                                                                                                  <  2:542285/140‑1 (MQ=255)

                                       cGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                              <  1:3061998/140‑1 (MQ=255)

                                       cGACTGTGATCGCAGAAAGACTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCACAAAACTAACTTTTTTATTGAGACGGCGACCTCCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                              <  2:3028797/140‑1 (MQ=255)

                                        gACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATCCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACg                                                                                             <  1:4428682/140‑1 (MQ=255)

                                          cTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCa                                                                                           <  2:879493/140‑1 (MQ=255)

                                           tgtgATCGCAGAAATCCTTTTTATTCCAGTCTTATACCCTGGCGTAAAAATCAAAAGAGTACCTGCTTTATTCTGGTGATAAGATTCACGATCTACACCAGTTCCAGCATCGTTCCTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  1:4523350/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCATACTTCTATCATCGCGTAAAACTCTAACAATTACCTTTTTTATTGTGATGACAACAACCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  2:2925625/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  1:2752350/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  1:2233412/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACAACAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  1:1326955/140‑1 (MQ=255)

                                               aTCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAATTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATGTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCa                                                                                      >  2:5007456/1‑140 (MQ=255)

                                               aTCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCa                                                                                      >  2:4325378/1‑140 (MQ=255)

                                                tCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCAt                                                                                     <  1:239486/140‑1 (MQ=255)

                                                   cAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACa                                                                                  >  2:4560805/1‑140 (MQ=255)

                                                    aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaa                                                                                 <  2:3641915/140‑1 (MQ=255)

                                                    aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaa                                                                                 >  2:3857112/1‑140 (MQ=255)

                                                           cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc                                                                          <  1:509400/140‑1 (MQ=255)

                                                           cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCAGGCGGCAACCACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc                                                                          <  2:1426082/140‑1 (MQ=255)

                                                             ttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccac                                                                        <  1:5111349/140‑1 (MQ=255)

                                                              tttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacg                                                                       >  1:2094418/1‑140 (MQ=255)

                                                                tcttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                     <  1:877307/140‑1 (MQ=255)

                                                                  ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAGAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  1:4672085/140‑1 (MQ=255)

                                                                  ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  1:2798769/140‑1 (MQ=255)

                                                                  ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  1:3613334/140‑1 (MQ=255)

                                                                  ttcCAGACTTCTATCAAGGCGTAAAAATCAAAAAATTACCTGTTTTAATCTTGCGGCGACATCCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  2:2800633/140‑1 (MQ=255)

                                                                   tcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccacg                                                                  <  1:5006655/140‑1 (MQ=255)

                                                                     cataCTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:2567564/137‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTGCGTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAACCTGCCCACGCCACggg                                                                <  1:1131627/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTTTTTAATGATACGGCGACCACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:2273802/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAACTTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:2041861/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:5594514/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:2202415/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:2671277/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:1202103/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:1670687/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:3381326/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:4616885/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:5300550/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:39202/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:4615218/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGCGGTGACAATCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:18729/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGATGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:4361414/140‑1 (MQ=255)

                                                                       gACTTCTATCATGGCGTAAAAATCAACAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGtt                                                              <  2:4591876/140‑1 (MQ=255)

                                                                                   ggCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATgtgt                                                  >  2:2018979/1‑140 (MQ=255)

                                                                                    gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtgtg                                                           <  1:583303/130‑1 (MQ=255)

                                                                                    gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtgtg                                                           >  2:583303/1‑130 (MQ=255)

                                                                                    gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTc                                                 >  2:1932262/1‑140 (MQ=255)

                                                                                    gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTc                                                 >  1:1551801/1‑140 (MQ=255)

                                                                                     cGTAAAAATCAAAAAATTACCTGTTTTAGTCTGGTGATAAACTTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCt                                                <  2:5084381/140‑1 (MQ=255)

                                                                                       tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCttt                                              >  2:3596484/1‑140 (MQ=255)

                                                                                            aTCAAAAAATTACCTGCTTTATTGTGGCGACAAACACCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCa                                         <  2:1790437/140‑1 (MQ=255)

                                                                                             tCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaa                                        <  1:1834718/140‑1 (MQ=255)

                                                                                                aaaaaTTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAgg                                     >  2:707358/1‑140 (MQ=255)

                                                                                                    aTTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGGTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCAtt                                 <  1:4079401/140‑1 (MQ=255)

                                                                                                     ttACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCAttt                                >  1:898359/1‑140 (MQ=255)

                                                                                                            cTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCgg                         <  1:142888/140‑1 (MQ=255)

                                                                                                            cTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCgg                         <  1:3857112/140‑1 (MQ=255)

                                                                                                                 ttCTGCTGATAAAGTTCACGATCGACGCGAGTGCCGGGATCGTTACTTCGGGGCGGCAGTTCAGGGGCATACCAGACGCACTGGCCACGCTACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg                    <  2:2679795/140‑1 (MQ=255)

                                                                                                                  tCTGGTGATACAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGt                   <  1:5476905/140‑1 (MQ=255)

                                                                                                                      gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACg               >  2:3301193/1‑140 (MQ=255)

                                                                                                                      gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACg               >  2:73722/1‑140 (MQ=255)

                                                                                                                      gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACg               >  1:4811982/1‑140 (MQ=255)

                                                                                                                      gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGGTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGGCGTAACg               >  2:3555843/1‑140 (MQ=255)

                                                                                                                       tggaaacaTTCGCGATCTACACCAGTTCCAGAATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACCAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt              <  2:91148/133‑1 (MQ=255)

                                                                                                                       tGATAACATCCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt              <  2:1282101/140‑1 (MQ=255)

                                                                                                                       cgATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt              <  2:4871693/139‑1 (MQ=255)

                                                                                                                         aTAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGttt            >  2:4547739/1‑140 (MQ=255)

                                                                                                                         aTAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGttt            >  1:450845/1‑140 (MQ=255)

                                                                                                                         aTAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGttt            <  1:1925709/140‑1 (MQ=255)

                                                                                                                           aaaaTTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTAt          >  1:2155958/1‑140 (MQ=255)

                                                                                                                            aaaTTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATc         <  1:5167738/140‑1 (MQ=255)

                                                                                                                                tCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTCTCTTCg     >  2:2644935/1‑140 (MQ=255)

                                                                                                                                 cACGATCTACCCCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTACTCCGGCGACGTAACGTTTATCTTCGa    <  1:67569/140‑1 (MQ=255)

                                                                                                                                  aCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAc   <  2:4114385/140‑1 (MQ=255)

                                                                                                                                   cGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACa  <  1:4848795/140‑1 (MQ=255)

                                                                                                                                   cGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACa  <  1:3200680/140‑1 (MQ=255)

                                                                                                                                   cGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACa  <  1:2018979/140‑1 (MQ=255)

                                                                                                                                   cGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACa  <  1:1005363/140‑1 (MQ=255)

ATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACA  >  NZ_CP025268/183952‑184222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`