BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,154,002	T→G	G341G (GGA→GGC)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,154,002	0	T	G	99.6%	915.7 / NA	277	G341G (GGA→GGC)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (102/174); minor base A (1/0); total (103/174)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

CCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTA  >  NZ_CP025268/2153865‑2154138

ccTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGcc                                                                                                                                        <  2:266202/140‑1 (MQ=255)

   aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                     <  2:1773686/140‑1 (MQ=255)

   aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                     >  2:2966967/1‑140 (MQ=255)

    aaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgaga                                                                                                                                    >  2:607689/1‑140 (MQ=255)

     aGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                   <  2:5466297/140‑1 (MQ=255)

     aGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                   <  1:2515827/140‑1 (MQ=255)

     aGGAACCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                   <  2:4283833/140‑1 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                               >  1:1982856/1‑140 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                               >  2:4613345/1‑140 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGACGAGATCAAt                                                                                                                               >  2:5392516/1‑140 (MQ=255)

          gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                              >  2:1762467/1‑140 (MQ=255)

          gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                              <  1:5194546/140‑1 (MQ=255)

          gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                              >  1:3087657/1‑140 (MQ=255)

              ccTGCCGGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATACTGGCGGTGCTACTGGCGCCATCACCATCGCCCGGACATTCGGCAGCACAAAATTCCCGCCGAGGGTAATGACCTCTTCGCCGAGTACAATAAAAt                                                                                                                          >  2:1617273/1‑140 (MQ=255)

              ccTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa                                                                                                                            >  2:4003863/1‑138 (MQ=255)

              ccTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa                                                                                                                            <  1:4003863/138‑1 (MQ=255)

              ccTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAAt                                                                                                                          <  2:1691629/140‑1 (MQ=255)

                  ccAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGAt                                                                                                                      <  2:4987755/140‑1 (MQ=255)

                   cAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATa                                                                                                                     <  1:1867983/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  2:3238099/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:3877367/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  2:3490539/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:1702190/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:4331217/140‑1 (MQ=255)

                       cGGGTGGAAATGATCGGGTAAGGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  2:4495493/140‑1 (MQ=255)

                                aTGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                        <  1:3267965/140‑1 (MQ=255)

                                 tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                       <  2:1013168/140‑1 (MQ=255)

                                 tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                       <  2:2701780/140‑1 (MQ=255)

                                 tGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                       <  1:3013904/140‑1 (MQ=255)

                                   aTCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCa                                                                                                     >  1:1449957/1‑140 (MQ=255)

                                   aTCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATGATCCa                                                                                                     >  2:4918067/1‑140 (MQ=255)

                                    tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACGTTCGCCAGCACAAAATACCCGCCGGTGGTAATGACCTCTTCGTTGAGATTAATAAAATTGATAACACCTTGATTAtggat                                                                                                    >  2:4673533/1‑136 (MQ=255)

                                       ggTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcgc                                                                                                 <  1:1014093/140‑1 (MQ=255)

                                         tAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcac                                                                                               <  2:1679151/140‑1 (MQ=255)

                                         tAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcac                                                                                               <  1:3941375/140‑1 (MQ=255)

                                         tAAGGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAACTTCCCGCCGGTGGTAATGACCTGTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcac                                                                                               <  2:2424421/140‑1 (MQ=255)

                                         caaTGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcac                                                                                               <  1:4926857/139‑1 (MQ=255)

                                                  ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                      >  1:2688647/1‑140 (MQ=255)

                                                    gaaaTGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGCGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                    <  2:3081774/138‑1 (MQ=255)

                                                    gCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                    <  2:1711712/140‑1 (MQ=255)

                                                    gCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                    <  2:1231315/140‑1 (MQ=255)

                                                         gCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagtcagt                                                                               <  2:4378744/140‑1 (MQ=255)

                                                            ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaa                                                                            >  2:2799962/1‑140 (MQ=255)

                                                            ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaa                                                                            >  1:1552260/1‑140 (MQ=255)

                                                            ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaa                                                                            >  1:5570382/1‑140 (MQ=255)

                                                            ggCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCaa                                                                            >  2:3949526/1‑140 (MQ=255)

                                                             gCGGTGCTACTGACGCCATCACCATCGCCCAGACTTGCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGGTTATCCCGCGCACACCACGGGCAGTCAGTCaaa                                                                           >  1:718737/1‑140 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGGCCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:3235941/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGCTCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCCCGGGCAGTCAGTCAAATGAg                                                                       <  2:1175642/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  2:506345/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  2:5574089/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:1836037/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:5192923/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:3475660/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAACGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  2:4932822/140‑1 (MQ=255)

                                                                  gCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAACATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGc                                                                      >  1:2772356/1‑140 (MQ=255)

                                                                     aCTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCggg                                                                   <  2:2137396/140‑1 (MQ=255)

                                                                      cTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCgggg                                                                  <  1:576919/140‑1 (MQ=255)

                                                                           gCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAAc                                                             >  1:4183505/1‑140 (MQ=255)

                                                                             cATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcagtca                                                                                 >  1:435444/1‑118 (MQ=255)

                                                                              aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                          <  1:4420065/140‑1 (MQ=255)

                                                                              aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                          <  1:2140581/140‑1 (MQ=255)

                                                                              aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                          <  2:4800413/140‑1 (MQ=255)

                                                                              aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                          >  1:5047145/1‑140 (MQ=255)

                                                                              aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                          >  2:806941/1‑140 (MQ=255)

                                                                                cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                        >  2:1892857/1‑140 (MQ=255)

                                                                                cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                        <  2:712445/140‑1 (MQ=255)

                                                                                aaCCATCGCCCAGATTTACGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTAGCCGAGATCAATAAAATAGATCACACCTTGATTAACCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                        <  2:3570784/139‑1 (MQ=255)

                                                                                       gCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGa                                                 <  1:4617927/140‑1 (MQ=255)

                                                                                       gCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGa                                                 >  1:230627/1‑140 (MQ=255)

                                                                                         ccAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGaaa                                               <  2:4069354/140‑1 (MQ=255)

                                                                                           aGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACg                                             >  1:1280133/1‑140 (MQ=255)

                                                                                             aCTTTCGCCAGCACCAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACAGGCATTGCTGAAACGAt                                           >  1:3117018/1‑140 (MQ=255)

                                                                                             aCTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGAt                                           >  2:3899470/1‑140 (MQ=255)

                                                                                               tttCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc                                         <  2:127344/140‑1 (MQ=255)

                                                                                               tttCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc                                         <  2:3411680/140‑1 (MQ=255)

                                                                                               tttCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc                                         >  1:5436020/1‑140 (MQ=255)

                                                                                               tttCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc                                         <  1:3808540/140‑1 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                          <  2:3109050/121‑1 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                                       <  1:2626891/140‑1 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                                       >  1:783917/1‑140 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                                       >  2:519561/1‑140 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                                       <  1:1605742/140‑1 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                                       <  1:3685917/140‑1 (MQ=255)

                                                                                                  cGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGAGTCAACATGCATTGCTGAAACGATACAtt                                      >  2:4702774/1‑140 (MQ=255)

                                                                                                       gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCGTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGGAAAGATGCAGGGCTGAAAGGATACATGTCttt                                 >  1:1613960/1‑140 (MQ=255)

                                                                                                        cacaAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTa                                <  2:1468488/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCTCGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:4105455/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:1100212/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:3485243/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:4319661/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:2886117/140‑1 (MQ=255)

                                                                                                              aTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGtt                          <  1:974472/140‑1 (MQ=255)

                                                                                                                 cccGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGc                       <  2:922635/140‑1 (MQ=255)

                                                                                                                      gggtcgtAATGAGCTCTTCGCCGAGATCAATAAAATAGATAGCGCCTTGATTATCCAGCGCACACCGCGGGCAGTAAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGGTACATTTCTTTATCTGTTGGCGTaga                  <  2:1599540/139‑1 (MQ=255)

                                                                                                                      cgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                  <  2:4452124/140‑1 (MQ=255)

                                                                                                                             aaTGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCa           <  2:201666/140‑1 (MQ=255)

                                                                                                                                   ctctTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCg     >  2:3208213/1‑140 (MQ=255)

                                                                                                                                     cttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCt   >  2:3134855/1‑140 (MQ=255)

                                                                                                                                     cttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCt   >  1:4736826/1‑140 (MQ=255)

CCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTA  >  NZ_CP025268/2153865‑2154138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`