BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,183,367	C→T	S170S (AGC→AGT)	yegT →	nucleoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,183,367	0	C	T	97.7%	773.0 / NA	220	S170S (AGC→AGT)	yegT	nucleoside permease
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (107/106); minor base G (5/0); total (113/107)
Fisher's exact test for biased strand distribution p-value = 6.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GCGTGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTCTT  >  NZ_CP025268/2183229‑2183504

 cGTGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttc                                                                                                                                         >  2:697202/1‑140 (MQ=255)

   tGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctg                                                                                                                                       >  2:85007/1‑140 (MQ=255)

    gATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctgc                                                                                                                                      <  1:1186589/140‑1 (MQ=255)

    gATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACACCCCGCTGCTGATTACCGCCGGAAgttctgc                                                                                                                                      <  1:1513198/140‑1 (MQ=255)

     ttttGCCGGGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAACTACTGGGGTATGCCGATATCTCCCCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctgct                                                                                                                                     <  2:1158576/139‑1 (MQ=255)

     aTTTCCCTCGCATTCGTGTGATGGGCACTATCGGCTGTATTGCCTCCTGTCTGGCATGTGGTGTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctgct                                                                                                                                     >  2:4383409/1‑140 (MQ=255)

     aTTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctgct                                                                                                                                     <  2:4215158/140‑1 (MQ=255)

     aTTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAgttctgct                                                                                                                                     >  1:1880277/1‑140 (MQ=255)

           cgcgCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCtgctctgctc                                                                                                                               >  1:3202864/1‑140 (MQ=255)

            gcgcATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCg                                                                                                                              <  1:3648245/140‑1 (MQ=255)

             cgcATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGTGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCgg                                                                                                                             >  1:1846882/1‑140 (MQ=255)

                  tCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGgtgtgt                                                                                                                        <  1:2552049/140‑1 (MQ=255)

                  tCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGgtgtgt                                                                                                                        <  1:4235726/140‑1 (MQ=255)

                         aTGGGCACTATCGACTAGATTGCCTCCGGACTGGCATGTGGTTTCTTGCCGCAAATAATGGGGTATGCCGATATCTCAACGACAAACATACCACTGCAGAGTACCGCCGGAAGGGCTGCGCGGCTCGGTGGGTTTgtgat                                                                                                                 >  2:639200/1‑136 (MQ=255)

                             gCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTc                                                                                                             <  1:3694045/140‑1 (MQ=255)

                              cACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTcc                                                                                                            >  2:3417813/1‑140 (MQ=255)

                              cACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTcc                                                                                                            >  1:1190585/1‑140 (MQ=255)

                               aCTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCt                                                                                                           >  2:5293272/1‑140 (MQ=255)

                                 tATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGc                                                                                                         >  1:2210956/1‑140 (MQ=255)

                                   tCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGccc                                                                                                       >  2:1194566/1‑140 (MQ=255)

                                    cGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAACTACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCGGATTACCGCCGGCAGTTCTGCGCTGCTCGGTGTGTTTGCGTTTTTCCTGccct                                                                                                      >  2:1676049/1‑139 (MQ=255)

                                      gCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCAGATATCTCCCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTTCTCTGCTCGGTGTGTTAGCGTTTTTCCTGCCCGac                                                                                                    >  2:4005650/1‑140 (MQ=255)

                                       cTGGATTGCCTCCGGTCTGGCATGTGGTTTCGTGCCGCAAATACTGGGGTATGCCGCTATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGaca                                                                                                   <  2:1020093/140‑1 (MQ=255)

                                        tGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGAGTTTTTCCTGCCCGacac                                                                                                  >  1:1434161/1‑140 (MQ=255)

                                          gATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                                <  2:3657314/140‑1 (MQ=255)

                                          gATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGc                                                                                                <  2:2396355/140‑1 (MQ=255)

                                           aTTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCGCACCGACTAACATCCCGCTGCTGATTACAGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGcc                                                                                               >  2:1466507/1‑140 (MQ=255)

                                               ccTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCg                                                                                                                              >  1:3873820/1‑105 (MQ=255)

                                               ccTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCg                                                                                                                              <  2:3873820/105‑1 (MQ=255)

                                                cTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCaa                                                                                          >  2:539742/1‑140 (MQ=255)

                                                 tCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCGGATAGCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCaaa                                                                                         <  1:5159164/140‑1 (MQ=255)

                                                       cTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCAc                                                                                   <  2:4422970/140‑1 (MQ=255)

                                                       cTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCAc                                                                                   <  2:3473634/140‑1 (MQ=255)

                                                       cTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCAc                                                                                   <  1:5515186/140‑1 (MQ=255)

                                                         ggCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCg                                                                                 >  2:2450980/1‑140 (MQ=255)

                                                         ggCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCg                                                                                 >  2:2706226/1‑140 (MQ=255)

                                                          gCATGTGGTGTCTTGCCGCAAATACTGGGGGATGCCGATATCTCACCGACCAACATCCCGCTGCTGATTACCGCCGGAGGTTCTGCTCCGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCgg                                                                                >  2:4625369/1‑140 (MQ=255)

                                                                ggTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                          <  1:2998480/140‑1 (MQ=255)

                                                                ggTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAAt                                                                          <  1:4752883/140‑1 (MQ=255)

                                                                 gTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATg                                                                         <  2:295896/140‑1 (MQ=255)

                                                                 gTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATg                                                                         <  1:84550/140‑1 (MQ=255)

                                                                 gTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATg                                                                         <  2:4073902/140‑1 (MQ=255)

                                                                 gTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATg                                                                         <  1:2781725/140‑1 (MQ=255)

                                                                    tCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGAAACGGCACCAAAAAGCACCGGCAACATGGat                                                                      >  2:2156970/1‑140 (MQ=255)

                                                                      ttGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGatat                                                                    <  2:2990826/140‑1 (MQ=255)

                                                                       tGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATAtt                                                                   <  1:1299230/140‑1 (MQ=255)

                                                                        gCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAGAATGGATATTa                                                                  <  2:3976213/140‑1 (MQ=255)

                                                                           gCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAg                                                               <  1:3536112/140‑1 (MQ=255)

                                                                                tACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATg                                                          <  2:1069418/140‑1 (MQ=255)

                                                                                  cTGGGGTGTGCCGATATCTCACCGTCTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  2:1383112/140‑1 (MQ=255)

                                                                                  cTGGGGTATGCCGATATCTCACCGACTCACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  1:4523546/140‑1 (MQ=255)

                                                                                  cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCTGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  2:3108294/140‑1 (MQ=255)

                                                                                  cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  1:816080/140‑1 (MQ=255)

                                                                                  cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  2:4870354/140‑1 (MQ=255)

                                                                                  cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  1:1384139/140‑1 (MQ=255)

                                                                                  cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  1:16435/140‑1 (MQ=255)

                                                                                  cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  1:2641288/140‑1 (MQ=255)

                                                                                  cTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCt                                                        <  1:4192992/140‑1 (MQ=255)

                                                                                    ggggTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCg                                                      >  1:4132926/1‑140 (MQ=255)

                                                                                       gTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                                   >  1:4163787/1‑140 (MQ=255)

                                                                                       gTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGcc                                                   >  2:3333060/1‑140 (MQ=255)

                                                                                         aTGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTg                                                 >  1:2413290/1‑140 (MQ=255)

                                                                                          tGCCGGTATATCACCGACTAACATCCCGCTGCTCATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTGTTTCCTGCCCGTCACGACGCCAAAAAGCACCGGCAAAATGGATATTCAAGTCATGCTGGGCCTgg                                                <  2:4439168/140‑1 (MQ=255)

                                                                                          tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                                >  1:671052/1‑140 (MQ=255)

                                                                                          tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                                >  1:4357834/1‑140 (MQ=255)

                                                                                          tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                                >  1:4732281/1‑140 (MQ=255)

                                                                                          tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                                >  1:5307897/1‑140 (MQ=255)

                                                                                          tGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCCTTTTTCCTGCCCTACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                                <  2:5373691/140‑1 (MQ=255)

                                                                                          tGCCAATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGGGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTgg                                                >  2:5148883/1‑140 (MQ=255)

                                                                                           tcctatatCTCACCGACTAACATCCCGCTGCTCATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCCCCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                               <  2:955883/136‑1 (MQ=255)

                                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                               >  1:4863595/1‑140 (MQ=255)

                                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                               >  1:1207432/1‑140 (MQ=255)

                                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                               >  1:2919251/1‑140 (MQ=255)

                                                                                           gCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGa                                               >  2:4140315/1‑140 (MQ=255)

                                                                                              gATATCTCACCGACTATCATCCCGCTGCTGACTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgc                                            <  1:3119420/140‑1 (MQ=255)

                                                                                               atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcg                                           >  1:4804609/1‑140 (MQ=255)

                                                                                               atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcg                                           >  1:5416089/1‑140 (MQ=255)

                                                                                               atatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCGCGGCCTGGATgcg                                           >  1:4924217/1‑140 (MQ=255)

                                                                                                tatCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATgcgc                                          >  2:4237093/1‑140 (MQ=255)

                                                                                                 atCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCt                                         >  2:3840283/1‑140 (MQ=255)

                                                                                                      aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTCAAGTCATGCTCGGCCTGGATGCGCTGATcc                                    >  2:1524080/1‑140 (MQ=255)

                                                                                                      aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc                                    >  1:3326298/1‑140 (MQ=255)

                                                                                                      aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc                                    >  2:2339929/1‑140 (MQ=255)

                                                                                                      aCCGACTAACATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATcc                                    >  2:4302398/1‑140 (MQ=255)

                                                                                                          aCTAACATCCCGCTGCTGACTACCGCCGGAAGTTCTGCTCTGCTCGGCGTGTTTGCGTTTTTCCTGCCCGACAGGCCACCCAGAAGCACCGGCAAAAGGGATGTTAAAGTCGTGCGCGGCCTGGATGCGCTGATCctgct                                >  2:4885543/1‑140 (MQ=255)

                                                                                                             aaCATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTgcg                             <  1:4563981/140‑1 (MQ=255)

                                                                                                              aCATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATTCTCGGCCTGGATGCGCTGATCCtgctgctg                            >  2:4280144/1‑138 (MQ=255)

                                                                                                               cATCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGcgcg                           <  2:3791934/140‑1 (MQ=255)

                                                                                                                aTCCCGCTGCTGATTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGa                          >  1:2460888/1‑140 (MQ=255)

                                                                                                                    cGCTGCTGATTACGGCCGGAGGGTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAATGCACCGGCAAAATGGATATTAAAGTCATGTTCGGCCTGGATGCGCTGATCCTGCTGCGCGATaaa                      >  2:1623732/1‑140 (MQ=255)

                                                                                                                            atcacggccgGAAGGTGTGATCTGCTCGGTGGGTTTGCGTCTTGCCGGCGCGACACGCCCCCAAAAAGCACCGGCCAAATGGATATTAAAGTCATGCTGGGCCTGGATGTGCTGATACTGCTGCGTGCTAAACACATCCt              >  2:2002867/7‑140 (MQ=255)

                                                                                                                            aTTACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACGCGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCt              <  1:4997869/140‑1 (MQ=255)

                                                                                                                              tcccgccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGGTATCAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg            <  2:3352685/138‑1 (MQ=255)

                                                                                                                              tACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg            <  1:2267836/140‑1 (MQ=255)

                                                                                                                              tACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg            <  1:2135620/140‑1 (MQ=255)

                                                                                                                              tACCGCCGGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCg            <  1:4455907/140‑1 (MQ=255)

                                                                                                                                ccgccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTc          <  1:701744/140‑1 (MQ=255)

                                                                                                                                 cgccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCt         >  1:1546561/1‑140 (MQ=255)

                                                                                                                                   ccgGAAGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCttt       >  1:5113560/1‑140 (MQ=255)

                                                                                                                                       aaGTTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTttct   <  1:3417813/140‑1 (MQ=255)

GCGTGATTTCCCGCGCATTCGTGTGATGGGCACTATCGGCTGGATTGCCTCCGGTCTGGCATGTGGTTTCTTGCCGCAAATACTGGGGTATGCCGATATCTCACCGACTAACATCCCGCTGCTGATTACCGCCGGAAGCTCTGCTCTGCTCGGTGTGTTTGCGTTTTTCCTGCCCGACACGCCACCAAAAAGCACCGGCAAAATGGATATTAAAGTCATGCTCGGCCTGGATGCGCTGATCCTGCTGCGCGATAAAAACTTCCTCGTCTTTTTCTT  >  NZ_CP025268/2183229‑2183504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`